{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T05:58:19Z","timestamp":1790315899927,"version":"4.1.0"},"reference-count":50,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T00:00:00Z","timestamp":1690502400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: Nontuberculous mycobacteria (NTM) are the cause of chronic lung disease called NTM lung disease (NTM-LD). There are about 180 known species of NTM. Nowadays the number of NTM-LD is increasing. Objective: To evaluate the clinical significance of NTM isolated from specimens and assess the frequency and clinical relevance of isolation of NTM in the Regional Center of Pulmonology in Bydgoszcz, hospital of Northern Poland. Design: Clinical, radiological, and microbiological data were collected from all patients from whom NTM was isolated between 2013 and 2022. Data were reviewed retrospectively. Diagnostic criteria for NTM-LD published by the American Thoracic Society (ATS) were used to determine clinical relevance. Material and methods: The study comprised 81,985 clinical specimens submitted for mycobacterial culture in the Department of Microbiology at the Regional Center of Pulmonology in Bydgoszcz between 2013 and 2022. Clinical specimens were processed according to the standard procedure in mycobacteria laboratories in Poland. NTM strains were identified using analysis of mycolic acids by chromatography as well as GenoType NTM-DR, GenoType Mycobacterium AS, and GenoType Mycobacterium CM. Results: There were 395 patients with NTM strains between 2013 and 2022. Out of them, 149 cases met the diagnostic criteria of NTM-LD and were classified as definite cases. M. kansasii (n = 77) was the most common species in the group (51.68%), followed by M. avium complex (n = 46). Patients with NTM-LD were 22\u201388 years old (median age was 60 years). There were 81 men and 68 women. The most common symptoms were cough, hemoptysis, and fever. Radiological X-ray images were dominated by infiltrative lesions in the upper and middle lobe of the right lung with cavities; the changes were in the upper lobe of the left lung and on both sides of the chest. They were smokers in 61%. The most common concomitant diseases were chronic obstructive pulmonary disease (COPD), diabetes mellitus, pulmonary carcinoma, and human immunodeficiency virus (HIV) infection, and other immunodeficiencies. The most common treatment was isoniazid, ethambutol, rifampicin, and ofloxacin for 18 months with a minimum of 12 months of culture negativity. Conclusions: NTM-LD infections are present with other pulmonary illnesses and extrapulmonary diseases and may be connected to primary immunologic deficiencies. These diseases concern patients of all ages and have various clinical manifestations. M. kansasii and MAC are the most prevalent NTM isolates among respiratory samples in Northern Poland. In addition, an increase in MAC and a decrease in M. kansasii both in cultivation and the cause of NTM-LD were reported.<\/jats:p>","DOI":"10.3390\/pathogens12080988","type":"journal-article","created":{"date-parts":[[2023,7,28]],"date-time":"2023-07-28T02:10:45Z","timestamp":1690510245000},"page":"988","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Trends from the Last Decade with Nontuberculous Mycobacteria Lung Disease (NTM-LD): Clinicians\u2019 Perspectives in Regional Center of Pulmonology in Bydgoszcz, Poland"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8324-163X","authenticated-orcid":false,"given":"Grzegorz","family":"Przybylski","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine and Lung Diseases, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus in Torun, 87-100 Toru\u0144, Poland"},{"name":"Regional Center of Pulmonology in Bydgoszcz, 85-326 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jakub","family":"Bukowski","sequence":"additional","affiliation":[{"name":"Regional Center of Pulmonology in Bydgoszcz, 85-326 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weronika","family":"Kowalska","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine and Lung Diseases, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus in Torun, 87-100 Toru\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Pilaczy\u0144ska-Cemel","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine and Lung Diseases, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus in Torun, 87-100 Toru\u0144, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Krawiecka","sequence":"additional","affiliation":[{"name":"Regional Center of Pulmonology in Bydgoszcz, 85-326 Bydgoszcz, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"102267","DOI":"10.1016\/j.tube.2022.102267","article-title":"Nontuberculous mycobacteria in the environment","volume":"137","author":"Falkinham","year":"2022","journal-title":"Tuberculosis"},{"key":"ref_2","unstructured":"(2021, April 06). List of Prokaryotic Names with Standing in Nomenclature-Genus Mycobacterium. Available online: https:\/\/lpsn.dsmz.de\/search?word=mycobacterium."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"777","DOI":"10.1148\/radiology.187.3.8497629","article-title":"Atypical mycobacterial infection in the lung: CT appearance","volume":"187","author":"Moore","year":"1993","journal-title":"Radiology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"968","DOI":"10.1016\/S1473-3099(15)00089-4","article-title":"Host susceptibility to non-tuberculous mycobacterial infections","volume":"15","author":"Wu","year":"2015","journal-title":"Lancet Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"185","DOI":"10.4103\/ijmr.IJMR_902_20","article-title":"Epidemiology, diagnosis & treatment of non-tuberculous mycobacterial diseases","volume":"152","author":"Sharma","year":"2020","journal-title":"Indian J. Med. Res."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1164\/rccm.201002-0310OC","article-title":"Nontuberculous mycobacterial lung disease prevalence at four integrated health care delivery systems","volume":"182","author":"Prevots","year":"2010","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1164\/rccm.201003-0503OC","article-title":"Pulmonary nontuberculous mycobacterial disease prevalence and clinical features: An emerging public health disease","volume":"182","author":"Winthrop","year":"2010","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1055\/s-0033-1333567","article-title":"Update on the epidemiology of pulmonary nontuberculous mycobacterial infections","volume":"34","author":"Kendall","year":"2013","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.3201\/eid2206.151642","article-title":"Prevalence of Nontuberculous Mycobacterial Pulmonary Disease, Germany, 2009\u20132014","volume":"22","author":"Ringshausen","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1164\/rccm.201111-2016OC","article-title":"Prevalence of nontuberculous mycobacterial lung disease in U","volume":"185","author":"Adjemian","year":"2012","journal-title":"S. Medicare beneficiaries. Am. J. Respir. Crit. Care Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1889","DOI":"10.3201\/eid1911.130737","article-title":"Pulmonary nontuberculous mycobacterial disease, Ontario, Canada, 1998\u20132010","volume":"19","author":"Marras","year":"2013","journal-title":"Emerg. Infect. Dis."},{"key":"ref_12","first-page":"210","article-title":"Nontuberculous mycobacterial pulmonary infections","volume":"6","author":"Johnson","year":"2014","journal-title":"J. Thorac. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1136\/thoraxjnl-2014-206470","article-title":"Increased risk of mycobacterial infections associated with anti-rheumatic medications","volume":"70","author":"Brode","year":"2015","journal-title":"Thorax"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"367","DOI":"10.1164\/rccm.200604-571ST","article-title":"An official ATS\/IDSA statement: Diagnosis, treatment, and prevention of nontuberculous mycobacterial diseases","volume":"175","author":"Griffith","year":"2007","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"94","DOI":"10.4046\/trd.2018.0026","article-title":"Infection Source and Epidemiology of Nontuberculous Mycobacterial Lung Disease","volume":"82","author":"Jeon","year":"2019","journal-title":"Tuberc. Respir. Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.3904\/kjim.2019.395","article-title":"Age- and sex-related characteristics of the increasing trend of nontuberculous mycobacteria pulmonary disease in a tertiary hospital in South Korea from 2006 to 2016","volume":"35","author":"Park","year":"2020","journal-title":"Korean J. Intern. Med."},{"key":"ref_17","unstructured":"Czarkowski, M.P., Niew\u0119g\u0142owska, A., Szmulik-Misiurek, K., and Zbrze\u017aniak, J. (2020). Choroby Zaka\u017ane i Zatrucia w Polsce w 2019, Narodowy Instytut Zdrowia Publicznego."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.jinf.2016.03.008","article-title":"Epidemiology of nontuberculous mycobacteria isolations among central North Carolina residents, 2006\u20132010","volume":"72","author":"Smith","year":"2016","journal-title":"J. Infect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.diagmicrobio.2011.09.009","article-title":"Pulmonary infection caused by nontuberculous mycobacteria in a medical center in Taiwan, 2005\u20132008","volume":"72","author":"Chen","year":"2012","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.3201\/eid1610.091201","article-title":"Changing epidemiology of pulmonary non- tuberculous mycobacteria infections","volume":"16","author":"Thomson","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1097\/QCO.0b013e328336ead6","article-title":"Non-tuberculous mycobacterial lung disease","volume":"23","author":"Griffith","year":"2010","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2000535","DOI":"10.1183\/13993003.00535-2020","article-title":"Treatment of nontuberculous mycobacterial pulmonary disease: An official ATS\/ERS\/ESCMID\/IDSA clinical practice guideline","volume":"56","author":"Daley","year":"2020","journal-title":"Eur. Respir. J."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"323","DOI":"10.5603\/ARM.34896","article-title":"Zalecenia PTChP dotycz\u0105ce rozpoznawania, leczenia i zapobiegania gru\u017alicy u doros\u0142ych i dzieci","volume":"81","author":"Demkow","year":"2013","journal-title":"Adv. Respir. Med."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1700374","DOI":"10.1183\/13993003.00374-2017","article-title":"Nontuberculous mycobacterial pulmonary disease: An increasing burden with substantial costs","volume":"49","author":"Prevots","year":"2017","journal-title":"Eur. Respir. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1513\/AnnalsATS.201709-727OC","article-title":"Epidemiology of pulmonary nontuberculous mycobacterial sputum positivity in patients with cystic fibrosis in the United States, 2010\u20132014","volume":"15","author":"Adjemian","year":"2018","journal-title":"Ann. Am. Thorac. Soc."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1513\/AnnalsATS.201605-353OC","article-title":"Increasing prevalence rate of nontuberculous mycobacteria infections in five states, 2008\u20132013","volume":"13","author":"Donohue","year":"2016","journal-title":"Ann. Am. Thorac. Soc."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Park, S.C., Kang, M.J., Han, C.H., Lee, S.M., Kim, C.J., Lee, J.M., and Kang, Y.A. (2019). Prevalence, incidence, and mortality of nontuberculous mycobacterial infection in Korea: A nationwide population-based study. BMC Pulm. Med., 19.","DOI":"10.1186\/s12890-019-0901-z"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1513\/AnnalsATS.201804-236OC","article-title":"Incidence and prevalence of nontuberculous mycobacterial lung disease in a.Large.U.S. Managed Care Health Plan, 2008\u20132015","volume":"17","author":"Winthrop","year":"2020","journal-title":"Ann. Am. Thorac. Soc."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"731","DOI":"10.1093\/cid\/ciy531","article-title":"Epidemiology of nontuberculous mycobacteria infection in children and young people with cystic fibrosis: Analysis of UK cystic fibrosis registry","volume":"68","author":"Gardner","year":"2019","journal-title":"Clin. Infect. Dis."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1016\/j.chest.2020.10.093","article-title":"Trends in the prevalence of non-TB mycobacterial infection in patients with non-cystic fibrosis bronchiectasis in South Korea, 2012\u20132016","volume":"159","author":"Lee","year":"2021","journal-title":"Chest"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"311","DOI":"10.3389\/fmed.2018.00311","article-title":"Increasing non-tuberculous mycobacteria infections in veterans with COPD and association with increased risk of mortality","volume":"5","author":"Pyarali","year":"2018","journal-title":"Front. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"771","DOI":"10.1378\/chest.129.3.771","article-title":"Pulmonary Mycobacterium kansasii infection in Israel, 1999\u20132004: Clinical features, drug susceptibility and outcome","volume":"129","author":"Shitrit","year":"2006","journal-title":"Chest"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"343","DOI":"10.3201\/eid170310060","article-title":"Non-tuberculous mycobacteria in respiratory tract infections, eastern Asia","volume":"17","author":"Simons","year":"2011","journal-title":"Emerg. Infect. Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1136\/thoraxjnl-2013-204260","article-title":"Non-tuberculous mycobacteria: A retrospective review of Scottish isolates from 2000 to 2010","volume":"69","author":"Russell","year":"2014","journal-title":"Thorax"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1183\/09031936.00149212","article-title":"The geographic diversity of nontuberculous mycobacteria isolated from pulmonary samples: An NTM-NET collaborative study","volume":"42","author":"Hoefsloot","year":"2013","journal-title":"Eur. Respir. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1055\/s-0038-1660864","article-title":"Geographic distribution of nontuberculous mycobacteria isolated from clinical specimens: A systematic review","volume":"39","author":"Zweijpfenning","year":"2018","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"514","DOI":"10.1164\/rccm.200905-0778OC","article-title":"Nontuberculous pulmonary mycobacteriosis in Denmark: Incidence and prognostic factors","volume":"181","author":"Thomsen","year":"2010","journal-title":"AM. J. Respir. Crit. Care Med."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"96","DOI":"10.5588\/ijtld.12.0237","article-title":"Epidemiology and clinical significance of non-tuberculous mycobacteria isolated from pulmonary specimens","volume":"17","author":"Braun","year":"2013","journal-title":"Int. J. Tuberc. Lung Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1055\/s-0041-111494","article-title":"Recommendations of the German Central Committee against Tuberculosis (DZK) and the German Respiratory Society (DGP) for the diagnosis and treatment of non-tuberculous mycobacterioses","volume":"70","author":"Schoenfeld","year":"2016","journal-title":"Pneumologie"},{"key":"ref_40","unstructured":"World Health Organization (2022). Global Tuberculosis Report, 2022, WHO."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"291","DOI":"10.5603\/ARM.a2018.0047","article-title":"Nontuberculous mycobacteria strains isolated from patients between 2013 and 2017 in Poland. Our data with respect to the global trends","volume":"86","author":"Kwiatkowska","year":"2018","journal-title":"Adv. Respir. Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.rmed.2019.05.001","article-title":"Interrelational changes in the epidemiology and clinical features of nontuberculous mycobacterial pulmonary disease and tuberculosis in a referral hospital in Japan","volume":"152","author":"Furuuchi","year":"2019","journal-title":"Respir. Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.3389\/fmicb.2018.02029","article-title":"Global environmental nontuberculous mycobacteria and their contemporaneous man-made and natural niches","volume":"9","author":"Honda","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_44","first-page":"PA2738","article-title":"Genotyping of human Mycobacterium kansasii isolates from Poland","volume":"50","author":"Proboszcz","year":"2017","journal-title":"Eur. Respir. J."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6696","DOI":"10.1038\/s41598-017-06931-4","article-title":"Nontuberculous mycobacteria in Denmark, incidence and clinical importance during the last quarter-century","volume":"7","author":"Hermansen","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1183\/09031936.99.13353599","article-title":"Pathological and radiological changes in resected lung specimens in Mycobacterium avium intracellulare complex disease","volume":"13","author":"Fujita","year":"1999","journal-title":"Eur. Respir. J."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"552","DOI":"10.3349\/ymj.2010.51.4.552","article-title":"Clinical characteristics and treatment outcomes of Mycobacterium kansasii lung disease in Korea","volume":"51","author":"Park","year":"2010","journal-title":"Yonsei Med. J."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"23","DOI":"10.3855\/jidc.1297","article-title":"Pulmonary disease caused by Mycobacterium simiae in Iran\u2019 s national referral center for tuberculosis","volume":"6","author":"Baghaei","year":"2012","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_49","unstructured":"Centers for Disease Control and Prevention (2008). Guidelines for Disinfection and Sterilization in Health Care Facilities, Centers for Disease Control and Prevention."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.ijmyco.2016.08.002","article-title":"Mycobacterial contamination of bronchoscopes: Challenges and possible solutions in low resource settings","volume":"5","author":"Saeed","year":"2016","journal-title":"Int. J. Mycobacteriol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/8\/988\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:21:09Z","timestamp":1760127669000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/8\/988"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,7,28]]},"references-count":50,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["pathogens12080988"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12080988","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,7,28]]}}}