{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:10:35Z","timestamp":1792789835055,"version":"4.5.0"},"reference-count":41,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T00:00:00Z","timestamp":1692230400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"Innovative Medicines 320 Initiative Joint Undertaking","doi-asserted-by":"publisher","award":["115721"],"award-info":[{"award-number":["115721"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"Innovative Medicines 320 Initiative Joint Undertaking","doi-asserted-by":"publisher","award":["603038"],"award-info":[{"award-number":["603038"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"Innovative Medicines 320 Initiative Joint Undertaking","doi-asserted-by":"publisher","award":["82DZL009B1"],"award-info":[{"award-number":["82DZL009B1"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Unions\u2019 Seventh Framework 322 Programme (FP&amp;\/2007-20130 and EFPIA companies\u2019 in-kind contribution and from the European Union\u2019s Seventh Framework Programme (FP7\/2007-2013)","doi-asserted-by":"publisher","award":["115721"],"award-info":[{"award-number":["115721"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Unions\u2019 Seventh Framework 322 Programme (FP&amp;\/2007-20130 and EFPIA companies\u2019 in-kind contribution and from the European Union\u2019s Seventh Framework Programme (FP7\/2007-2013)","doi-asserted-by":"publisher","award":["603038"],"award-info":[{"award-number":["603038"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Unions\u2019 Seventh Framework 322 Programme (FP&amp;\/2007-20130 and EFPIA companies\u2019 in-kind contribution and from the European Union\u2019s Seventh Framework Programme (FP7\/2007-2013)","doi-asserted-by":"publisher","award":["82DZL009B1"],"award-info":[{"award-number":["82DZL009B1"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"name":"German Research Foundation (SFB-TR84, sub-project B08 and SFB-1449, project 431232613, sub-project Z02) and the German Federal Ministry of Education and Research","award":["115721"],"award-info":[{"award-number":["115721"]}]},{"name":"German Research Foundation (SFB-TR84, sub-project B08 and SFB-1449, project 431232613, sub-project Z02) and the German Federal Ministry of Education and Research","award":["603038"],"award-info":[{"award-number":["603038"]}]},{"name":"German Research Foundation (SFB-TR84, sub-project B08 and SFB-1449, project 431232613, sub-project Z02) and the German Federal Ministry of Education and Research","award":["82DZL009B1"],"award-info":[{"award-number":["82DZL009B1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen and the leading cause of infection in patients with cystic fibrosis (CF). The ability of P. aeruginosa to evade host responses and develop into chronic infection causes significant morbidity and mortality. Several mouse models have been developed to study chronic respiratory infections induced by P. aeruginosa, with the bead agar model being the most widely used. However, this model has several limitations, including the requirement for surgical procedures and high mortality rates. Herein, we describe novel and adapted biologically relevant models of chronic lung infection caused by P. aeruginosa. Three methods are described: a clinical isolate infection model, utilising isolates obtained from patients with CF; an incomplete antibiotic clearance model, leading to bacterial bounce-back; and the establishment of chronic infection; and an adapted water bottle chronic infection model. These models circumvent the requirement for a surgical procedure and, importantly, can be induced with clinical isolates of P. aeruginosa and in wild-type mice. We also demonstrate successful induction of chronic infection in the transgenic \u03b2ENaC murine model of CF. We envisage that the models described will facilitate the investigations of host and microbial factors, and the efficacy of novel antimicrobials, during chronic P. aeruginosa respiratory infections.<\/jats:p>","DOI":"10.3390\/pathogens12081053","type":"journal-article","created":{"date-parts":[[2023,8,17]],"date-time":"2023-08-17T10:15:48Z","timestamp":1692267348000},"page":"1053","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Biologically Relevant Murine Models of Chronic Pseudomonas aeruginosa Respiratory Infection"],"prefix":"10.3390","volume":"12","author":[{"given":"Aoife M.","family":"Rodgers","sequence":"first","affiliation":[{"name":"Wellcome-Wolfson Institute of Experimental Medicine, Queen\u2019s University Belfast, Belfast BT7 1NN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Lindsay","sequence":"additional","affiliation":[{"name":"Wellcome-Wolfson Institute of Experimental Medicine, Queen\u2019s University Belfast, Belfast BT7 1NN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avril","family":"Monahan","sequence":"additional","affiliation":[{"name":"Wellcome-Wolfson Institute of Experimental Medicine, Queen\u2019s University Belfast, Belfast BT7 1NN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice V.","family":"Dubois","sequence":"additional","affiliation":[{"name":"Wellcome-Wolfson Institute of Experimental Medicine, Queen\u2019s University Belfast, Belfast BT7 1NN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aduragbemi A.","family":"Faniyi","sequence":"additional","affiliation":[{"name":"Wellcome-Wolfson Institute of Experimental Medicine, Queen\u2019s University Belfast, Belfast BT7 1NN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry J.","family":"Plant","sequence":"additional","affiliation":[{"name":"Cork Centre for Cystic Fibrosis (3CF), Cork University Hospital, University College Cork, T12 E8YV Cork, Ireland"},{"name":"The HRB Funded Clinical Research Facility, University College Cork, T12 E8YV Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4057-2199","authenticated-orcid":false,"given":"Marcus A.","family":"Mall","sequence":"additional","affiliation":[{"name":"Department of Pediatric Respiratory Medicine, Immunology and Critical Care Medicine, Charit\u00e9\u2014University of Medicine Berlin, 10117 Berlin, Germany"},{"name":"German Center for Lung Research (DZL), 10117 Berlin, Germany"},{"name":"Berlin Institute of Health at Charit\u00e9\u2014University of Medicine Berlin, 10117 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miquel B.","family":"Ekkelenkamp","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, 3584 CX Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart","family":"Elborn","sequence":"additional","affiliation":[{"name":"Wellcome-Wolfson Institute of Experimental Medicine, Queen\u2019s University Belfast, Belfast BT7 1NN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1832-2457","authenticated-orcid":false,"given":"Rebecca J.","family":"Ingram","sequence":"additional","affiliation":[{"name":"Wellcome-Wolfson Institute of Experimental Medicine, Queen\u2019s University Belfast, Belfast BT7 1NN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bhagirath, A.Y., Li, Y., Somayajula, D., Dadashi, M., Badr, S., and Duan, K. 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