{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T08:16:26Z","timestamp":1781165786441,"version":"3.54.1"},"reference-count":54,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T00:00:00Z","timestamp":1692316800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais (FAPEMIG)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Background: Porteirinha is endemic for visceral leishmaniasis (VL), with intense disease transmission of the disease. We evaluated the impact of canine euthanasia as a single control measure on the incidence of VL in humans and canines. Methods: A prospective observational cohort study was carried out over four years (1998\u20132002) in 8 of the 12 neighborhoods of the city. The dynamics of canine visceral leishmaniasis (CVL) transmission were evaluated for 2 years, before beginning the screening\u2013culling intervention. The comparative morbidity index (CMI) was used to stratify areas with the greatest risk of CVL, and the spatial distribution of human and canine VL cases was compared using univariate and bivariate K-functions. Results: Human cases conglomerated in three neighborhoods. Spatial clusters were detected for CVL in 1998, 2000, and 2001, but not in 1999, when greater spatial dispersion occurred. The screening and culling intervention reduced the number of human VL cases and decreased the incidence of CVL, mainly in neighborhoods with a high CMI. Conclusions: The systematic euthanasia of seropositive dogs was shown to be an effective control action of the Program for Control of Visceral Leishmaniasis (PCLV) in Brazil. The fundamental role of domestic dogs in the epidemiological chain of VL was reaffirmed.<\/jats:p>","DOI":"10.3390\/pathogens12081060","type":"journal-article","created":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T10:28:48Z","timestamp":1692354528000},"page":"1060","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["The Program for the Control of Visceral Leishmaniasis in Brazil: The Effect of the Systematic Euthanasia of Seropositive Dogs as a Single Control Action in Porteirinha, a Brazilian City with an Intense Transmission of Visceral Leishmaniasis"],"prefix":"10.3390","volume":"12","author":[{"given":"Jo\u00e3o Carlos","family":"Fran\u00e7a-Silva","sequence":"first","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4181-7546","authenticated-orcid":false,"given":"Rodolfo Cordeiro","family":"Giunchetti","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reysla Maria da Silveira","family":"Mariano","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte 31270-901, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9806-9721","authenticated-orcid":false,"given":"George Luiz Lins","family":"Machado-Coelho","sequence":"additional","affiliation":[{"name":"Medicine School, Federal University of Ouro Preto, Ouro Preto 35402-163, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana de Almeida Silva","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Medical Clinics, Federal University of Tri\u00e2ngulo Mineiro, Uberaba 38025-180, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ricardo Andrade","family":"Barata","sequence":"additional","affiliation":[{"name":"Federal University of Vales do Jequitinhonha e Mucuri, Diamantina 39803-371, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c9rika Monteiro","family":"Michalsky","sequence":"additional","affiliation":[{"name":"Ren\u00e9 Rachou Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00edlia Fonseca","family":"Rocha","sequence":"additional","affiliation":[{"name":"Zoonoses Control Center, State University of Montes Claros, Montes Claros 39401-089, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Consuelo Latorre","family":"Fortes-Dias","sequence":"additional","affiliation":[{"name":"Research and Development Center, Ezequiel Dias Foundation, Belo Horizonte 30510-010, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2370-5113","authenticated-orcid":false,"given":"Edelberto Santos","family":"Dias","sequence":"additional","affiliation":[{"name":"Ren\u00e9 Rachou Institute, Oswaldo Cruz Foundation, Belo Horizonte 30190-002, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,8,18]]},"reference":[{"key":"ref_1","first-page":"67","article-title":"Controle da leishmaniose visceral no Brasil","volume":"27","author":"Monteiro","year":"1994","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1590\/S0074-02762009000100009","article-title":"Phlebotominae distribution in Jana\u00faba, an area of transmission for visceral leishmaniasis in Brazil","volume":"104","author":"Michalsky","year":"2009","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_3","first-page":"44","article-title":"Vigil\u00e2ncia e controle da leishmaniose visceral no contexto urbano","volume":"65","author":"Morais","year":"2012","journal-title":"Cad. T\u00e9c. Vet. Zootec."},{"key":"ref_4","unstructured":"Peters, W., and Killick-Kendrick, R. (1987). The Leishmaniasis in Biology and Medicine, Academic Press."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"510","DOI":"10.4269\/ajtmh.2001.65.510","article-title":"Impact of canine control on the epidemiology of canine and human visceral leishmaniasis in Brazil","volume":"65","author":"Reis","year":"2001","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_6","unstructured":"Minist\u00e9rio da Sa\u00fade (MS) Brasil, and Secretaria de Vigil\u00e2ncia em Sa\u00fade (2006). Manual de Vigil\u00e2ncia e Controle da Leishmaniose Visceral, Minist\u00e9rio da Sa\u00fade."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"287","DOI":"10.4269\/ajtmh.1995.52.287","article-title":"Control of zoonotic visceral leishmaniasis: Is it time to change strategies?","volume":"52","author":"Tesh","year":"1995","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1240","DOI":"10.1086\/516096","article-title":"Effect of eliminating seropositive canines on the transmission of visceral leishmaniasis in Brazil","volume":"25","author":"Dietze","year":"1997","journal-title":"Clin. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"464","DOI":"10.1016\/S0035-9203(99)90340-1","article-title":"Is the household dog a risk factor for American visceral leishmaniasis in Brazil?","volume":"93","author":"Costa","year":"1999","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1086\/344312","article-title":"Infectiousness in a cohort of Brazilian dogs: Why culling fails to control visceral leishmaniasis in areas of high transmission","volume":"186","author":"Courtenay","year":"2002","journal-title":"J. Infect. Dis."},{"key":"ref_11","first-page":"197","article-title":"Kala-azar in the Rio Doce, Minas Gerais area. Results of prophylactic measures","volume":"22","author":"Magalhaes","year":"1980","journal-title":"Rev. Inst. Med. Trop. Sao Paulo"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"386","DOI":"10.1590\/S0037-86822011000300025","article-title":"Controle da leishmaniose visceral no munic\u00edpio de Porteirinha, estado de Minas Gerais, no per\u00edodo de 1998 a 2003","volume":"44","author":"Barata","year":"2011","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"106305","DOI":"10.1016\/j.actatropica.2022.106305","article-title":"Impact of vector control actions in the abundance of Lutzomyia longipalpis in Montes Claros, Brazil","volume":"228","author":"Rocha","year":"2022","journal-title":"Acta Trop."},{"key":"ref_14","unstructured":"(1992). Informe Epidemiol\u00f3gico do SUS\/Funda\u00e7\u00e3o Nacional da Sa\u00fade, Funda\u00e7\u00e3o Nacional de Sa\u00fade Cenepi."},{"key":"ref_15","first-page":"213","article-title":"Importance of Lutzomyia longipalpis in the dynamics of transmission of canine visceral leishmaniasis in the endemic area of Porteirinha municipality, Minas Gerais, Brazil","volume":"13","author":"Barata","year":"2005","journal-title":"Vet. Parasitol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1590\/S0074-02761985000100003","article-title":"A urgery for American cutaneous and visceral leishmaniasis among 1342 dogs from areas in Rio de Janeiro (Brazil) where the human diseases occur","volume":"80","author":"Coutinho","year":"1985","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1016\/S0304-4017(02)00351-5","article-title":"Epidemiology of canine visceral leishmaniosis in the endemic area of Montes Claros municipality, Minas Gerais State, Brazil","volume":"111","author":"Siqueira","year":"2003","journal-title":"Vet. Parasitol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"500","DOI":"10.4269\/ajtmh.1969.18.500","article-title":"Cross-reactivity in fluorescence tests for Trypanosoma and Leishmania antibodies: A simple inhibition procedure to ensure specific results","volume":"18","author":"Camargo","year":"1969","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1590\/S0037-86821991000100004","article-title":"Leishmaniose visceral canina: Avalia\u00e7\u00e3o da metodologia sorol\u00f3gica utilizada em inqu\u00e9ritos epidemiol\u00f3gicos","volume":"24","author":"Genaro","year":"1991","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1590\/S0074-02762005000200015","article-title":"Evaluation of enzyme-linked immunosorbent assay using crude Leishmania and recombinant antigens as a diagnostic marker for canine visceral leishmaniasis","volume":"100","author":"Genaro","year":"2005","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1590\/S0034-89101996000200007","article-title":"Morbidade referida e utiliza\u00e7\u00e3o de servi\u00e7os de sa\u00fade em localidades urbanas brasileiras: Metodologia","volume":"30","author":"Cesar","year":"1996","journal-title":"Rev. Sa\u00fade P\u00fablica"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"118","DOI":"10.4269\/ajtmh.1990.42.118","article-title":"Canine visceral leishmaniasis in northeast Brazil: Assessment of serodiagnostic methods","volume":"42","author":"Evans","year":"1990","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1590\/S0074-02761991000400006","article-title":"Serological survey for canine cutaneous and visceral leishmaniasis in areas at risk for transmission in Rio de Janeiro where prophylatic measures had been adopted","volume":"86","author":"Nunes","year":"1991","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_24","first-page":"125","article-title":"Canine visceral leishmaniasis in the Great Athens area, Greece","volume":"3","author":"Sideris","year":"1996","journal-title":"Parasite"},{"key":"ref_25","unstructured":"Ranque, J.M., and Quilici, D. (1977). Les Leishmanioses de la Regi\u00f3n Provencale: Considerations Epidemiologiques et Ecologiques, Centre National de la Recherche Scientifique. Colloques Internationaux du CNRS, no. 239, Ecologie des leishmanioses."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1017\/S0031182000073662","article-title":"Epidemiology of canine leishmaniasis: Prevalence, incidence and basic reproduction number calculated from a cross-sectional serological survey on the Island of Gozo, Malta","volume":"105","author":"Dye","year":"1992","journal-title":"Parasitology"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1017\/S0031182000073674","article-title":"Mathematical modelling and theory for estimating the basic reproduction number of canine leishmaniasis","volume":"105","author":"Hasibeder","year":"1992","journal-title":"Parasitology"},{"key":"ref_28","first-page":"383","article-title":"Leishmaniasis in Tuscany (Italy): VI. Canine Leishmaniasis in the Focus of Monte Argentario (Grosseto)","volume":"38","author":"Pozio","year":"1981","journal-title":"Acta Trop."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"557","DOI":"10.2307\/3283159","article-title":"Canine leishmaniasis: Pathological and ecological factors influencing transmission of infection","volume":"77","author":"Abranches","year":"1991","journal-title":"J. Parasitol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1051\/parasite\/1975501001","article-title":"Ecology of leishmaniasis in the south of France. 8. Complement to the epidemiological application of the immunofluorescence technic: Geometric and arithmetic mean titers in canine leishmaniasis","volume":"50","author":"Lanotte","year":"1975","journal-title":"Ann. Parasitol. Hum. Comp."},{"key":"ref_31","first-page":"85","article-title":"Visceral leishmaniasis or kala-azar: The epidemiological and control aspects","volume":"31","author":"Vieira","year":"1998","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"S359","DOI":"10.1590\/S0102-311X1994000800014","article-title":"Tegumentary and visceral leishmaniases in Brazil: Emerging anthropozoonosis and possibilities for their control","volume":"10","author":"Marzoch","year":"1994","journal-title":"Cad. Sa\u00fade P\u00fablica"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1590\/S0037-86821990000200011","article-title":"Ocorr\u00eancia de calazar em \u00e1rea urbana da grande Belo Horizonte, MG","volume":"23","author":"Genaro","year":"1990","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_34","unstructured":"Chang, K.P., and Bray, R.S. (1985). Leishmaniasis, Elsevier."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1093\/infdis\/154.4.639","article-title":"A prospective study of visceral leishmaniasis in an endemic area of Brazil","volume":"154","author":"Badaro","year":"1986","journal-title":"J. Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1098\/rspb.1993.0123","article-title":"Malnutrition, age and the risk of parasitic disease: Visceral leishmaniasis revisited","volume":"254","author":"Dye","year":"1993","journal-title":"Proc. R. Soc. Lond. B"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1590\/S0074-02761996000600003","article-title":"Ecological interactions of visceral leishmaniasis in the state of Bahia, Brazil","volume":"91","author":"Sherlock","year":"1996","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.1590\/S0102-311X2001000500023","article-title":"Spatial distribution of human and canine visceral leishmaniasis in Belo Horizonte, Minas Gerais State, Brasil, 1994\u20131997","volume":"17","author":"Oliveira","year":"2001","journal-title":"Cad. Sa\u00fade P\u00fablica"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1590\/S0074-02762004000500004","article-title":"Phlebotomine sand flies in Porteirinha, an area of American visceral leishmaniasis transmission in the state of Minas Gerais, Brazil","volume":"99","author":"Barata","year":"2004","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1590\/S0037-86822005000500012","article-title":"Aspectos da ecologia e do comportamento de flebotom\u00edneos em \u00e1rea end\u00eamica de leishmaniose visceral, Minas Gerais","volume":"38","author":"Barata","year":"2005","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"53","DOI":"10.4269\/ajtmh.1998.59.53","article-title":"Studies on control of visceral leishmaniasis: Impact of dog control on canine and human visceral leishmaniasis in Jacobina, Bahia, Brazil","volume":"59","author":"Ashford","year":"1998","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"125","DOI":"10.4269\/ajtmh.1996.55.125","article-title":"The logic of visceral leishmaniasis control","volume":"55","author":"Dye","year":"1996","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1590\/S0037-86821998000500001","article-title":"Controle do calazar canino: Compara\u00e7\u00e3o dos resultados de um programa de elimina\u00e7\u00e3o r\u00e1pida de c\u00e3es sororreagentes por ensaio imuno-enzim\u00e1tico com outro de elimina\u00e7\u00e3o tardia de c\u00e3es sororreagentes por teste de imunofluoresc\u00eancia indireta de eluato de papel filtro","volume":"31","author":"Braga","year":"1998","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_44","first-page":"175","article-title":"Profilaxia do calazar no Cear\u00e1, Brazil","volume":"3","author":"Alencar","year":"1961","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"ref_45","unstructured":"Minist\u00e9rio da Sa\u00fade (MS) Brasil, and Secretaria de Vigil\u00e2ncia em Sa\u00fade (2022, December 08). Manual de Vigi\u00e2lncia e Controle da Leishmaniose Visceral, Available online: https:\/\/bvsms.saude.gov.br\/bvs\/publicacoes\/guia_vigilancia_saude_5ed.pdf."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1590\/S0001-37652004000300012","article-title":"Improving methods for epidemiological control of canine visceral leishmaniasis based on a mathematical model. Impact on the incidence of the canine and human disease","volume":"76","author":"Palatnik","year":"2004","journal-title":"Acad. Bras. Cienc."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Costa, D.N., Codec\u0327o, C.T., Silva, M.A., and Werneck, G.L. (2013). Culling dogs in scenarios of imperfect control: Realistic impact on the prevalence of canine visceral leishmaniasis. PLoS Negl. Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002355"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1590\/S0037-86822011005000014","article-title":"How effective is dog culling in controlling zoonotic visceral leishmaniasis? A critical evaluation of the science, politics and ethics behind this public health policy","volume":"44","author":"Costa","year":"2011","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Rocha, M.F., Michalsky, \u00c9.M., de Oliveira Lara-Silva, F., Valad\u00e3o, J.L., Fran\u00e7a-Silva, J.C., Pinheiro, L.C., de Sousa, J.F., Dos Santos, R.C., Soares, M.D., and Fortes-Dias, C.L. (2020). Dogs with divergent serology for visceral leishmaniasis as sources of Leishmania infection for Lutzomyia longipalpis phlebotomine sand flies\u2014An observational study in an endemic area in Brazil. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008079"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1016\/j.vetpar.2013.03.017","article-title":"Asymptomatic dogs are highly competent to transmit Leishmania (Leishmania) infantum chagasi to the natural vector","volume":"196","author":"Laurenti","year":"2013","journal-title":"Vet. Parasitol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.vetpar.2010.11.014","article-title":"Eco-epidemiology of visceral leishmaniasis in the urban area of Paracatu, state of Minas Gerais, Brazil","volume":"176","author":"Dias","year":"2011","journal-title":"Vet. Parasitol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.actatropica.2015.04.002","article-title":"Epidemiological aspects of vector, parasite, and domestic reservoir in areas of recent transmission and no reported human cases of visceral leishmaniasis in Brazil","volume":"148","author":"Michalsky","year":"2015","journal-title":"Acta Trop."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"70","DOI":"10.1016\/j.ijvsm.2017.02.002","article-title":"Seroprevalence and molecular characterization of Leishmaniain dogs from an endemic area of zoonotic visceral leishmaniasis in Brazil","volume":"5","author":"Lopes","year":"2017","journal-title":"Int. J. Vet. Sci. Med."},{"key":"ref_54","unstructured":"Minist\u00e9rio da Sa\u00fade (MS) Brasil, and Secretaria de Vigil\u00e2ncia em Sa\u00fade (2022, December 08). Nota T\u00e9cnica No 5\/2021-CGZV\/DEIDT\/SVS\/MS, Available online: https:\/\/www.gov.br\/saude\/pt-br\/assuntos\/saude-de-a-a-z\/l\/leishmaniose-visceral\/arquivos\/sei_ms-nota-tecnica-n-5_leishpdf.pdf."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/8\/1060\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:37:03Z","timestamp":1760128623000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/8\/1060"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,8,18]]},"references-count":54,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2023,8]]}},"alternative-id":["pathogens12081060"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12081060","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,8,18]]}}}