{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:28:53Z","timestamp":1760149733380,"version":"build-2065373602"},"reference-count":65,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T00:00:00Z","timestamp":1693958400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"SECRETAR\u00cdA DE INVESTIGACI\u00d3N Y POSGRADO from INSTITUTO POLIT\u00c9CNICO NACIONAL","award":["SIP-20140955","SIP-20181010","60883"],"award-info":[{"award-number":["SIP-20140955","SIP-20181010","60883"]}]},{"name":"CONSEJO NACIONAL DE HUMANIDADES, CIENCIA Y TECNOLOG\u00cdAS (CONAHCyT)","award":["SIP-20140955","SIP-20181010","60883"],"award-info":[{"award-number":["SIP-20140955","SIP-20181010","60883"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Arboviruses are an important group of pathogens that cause diseases of medical and veterinary concern worldwide. The interactions of these viruses with their host cells are complex, and frequently, the coexistence of two different viruses in the same cell results in the inhibition of replication in one of the viruses, which is a phenomenon called viral interference. This phenomenon can be exploited to develop antiviral strategies. Insect cell lines persistently infected with arboviruses are useful models with which to study viral interference. In this work, a model of C6\/36-HT cells (from Aedes albopictus mosquitoes) persistently infected with Dengue virus, serotype 2, was used. Viral interference was evaluated via plaque and flow cytometry assays. The presence of heterotypic interference against the other serotypes of the same virus and homologous interference against yellow fever virus was determined; however, this cell line did not display heterologous viral interference against Sindbis virus. The mechanisms responsible for viral interference have not been fully elucidated, but small RNAs could be involved. However, the silencing of Ago3, a key protein in the genome-derived P-element-induced wimpy testis pathway, did not alter the viral interference process, suggesting that viral interference occurs independent of this pathway.<\/jats:p>","DOI":"10.3390\/pathogens12091135","type":"journal-article","created":{"date-parts":[[2023,9,6]],"date-time":"2023-09-06T10:07:37Z","timestamp":1693994857000},"page":"1135","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of Viral Interference in Aedes albopictus C6\/36 Cells Persistently Infected with Dengue Virus 2"],"prefix":"10.3390","volume":"12","author":[{"given":"Aurora Montsserrat","family":"Gonz\u00e1lez-Flores","sequence":"first","affiliation":[{"name":"Centro de Estudios Cient\u00edficos y Tecnol\u00f3gicos del Estado de Tlaxcala, Tlaxcala 90491, Tlax., Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mariana","family":"Salas-Benito","sequence":"additional","affiliation":[{"name":"Escuela Nacional de Medicina y Homeopat\u00eda, Instituto Polit\u00e9cnico Nacional, Mexico City 07320, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victor Hugo","family":"Rosales-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Laboratorios Centrales, Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Mexico City 07360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5639-9503","authenticated-orcid":false,"given":"Paola Berenice","family":"Z\u00e1rate-Segura","sequence":"additional","affiliation":[{"name":"Escuela Superior de Medicina, Instituto Polit\u00e9cnico Nacional, Mexico City 11340, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6785-2035","authenticated-orcid":false,"given":"Rosa Mar\u00eda","family":"Del \u00c1ngel","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular, Centro de Investigaci\u00f3n y de Estudios Avanzados del IPN, Mexico City 07360, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nica Ascenci\u00f3n","family":"De Nova-Ocampo","sequence":"additional","affiliation":[{"name":"Escuela Nacional de Medicina y Homeopat\u00eda, Instituto Polit\u00e9cnico Nacional, Mexico City 07320, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan Santiago","family":"Salas-Benito","sequence":"additional","affiliation":[{"name":"Escuela Nacional de Medicina y Homeopat\u00eda, Instituto Polit\u00e9cnico Nacional, Mexico City 07320, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,9,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1186\/1756-3305-3-95","article-title":"Evidence of co-infection of chikungunya and densonucleosis viruses in C6\/36 cell lines and laboratory infected Aedes aegypti (L.) mosquitoes","volume":"3","author":"Sivaram","year":"2010","journal-title":"Parasites Vectors"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1006\/viro.1997.8492","article-title":"Multiple Mechanisms for the Inhibition of Entry and Uncoating of Superinfecting Semliki Forest Virus","volume":"231","author":"Singh","year":"1997","journal-title":"Virology"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1016\/j.virol.2004.08.032","article-title":"Markedly reduced severity of Dengue virus infection in mosquito cell cultures persistently infected with Aedes albopictus densovirus (AalDNV)","volume":"329","author":"Burivong","year":"2004","journal-title":"Virology"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"7119","DOI":"10.1128\/jvi.71.9.7119-7123.1997","article-title":"Superinfection exclusion of alphaviruses in three mosquito cell lines persistently infected with Sindbis virus","volume":"71","author":"Karpf","year":"1997","journal-title":"J. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1099\/0022-1317-59-2-273","article-title":"Demonstration of Interference between Dengue Virus Types in Cultured Mosquito Cells using Monoclonal Antibody Probes","volume":"59","author":"Dittmar","year":"1982","journal-title":"J. Gen. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1128\/CMR.13.4.651","article-title":"Molecular Strategies for Interrupting Arthropod-Borne Virus Transmission by Mosquitoes","volume":"13","author":"Blair","year":"2000","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1111\/j.1749-6632.1975.tb35103.x","article-title":"Observations of Aedes albopictus cell cultures persistently infected with Sindbis virus","volume":"266","author":"Stollar","year":"1975","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1128\/jvi.23.3.554-561.1977","article-title":"Role of extracellular virus on the maintenance of the persistent infection induced in Aedes albopictus (mosquito) cells by Sindbis virus","volume":"23","author":"Riedel","year":"1977","journal-title":"J. Virol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/0042-6822(77)90033-2","article-title":"Evolution and properties of Aedes albopictus cell cultures persistently infected with sindbis virus","volume":"82","author":"Igarashi","year":"1977","journal-title":"Virology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1038\/280690a0","article-title":"Characteristics of Aedes albopictus cells persistently infected with dengue viruses","volume":"280","author":"Igarashi","year":"1979","journal-title":"Nature"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1007\/BF01318104","article-title":"Production of temperature-sensitive and pathogenic virus from Aedes albopictus cells (Singh) persistently infected with chikungunya virus","volume":"62","author":"Igarashi","year":"1979","journal-title":"Arch. Virol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1016\/0042-6822(81)90070-2","article-title":"Mosquito cells infected with banzi virus secrete an antiviral activity which is of viral origin","volume":"110","author":"Lee","year":"1981","journal-title":"Virology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1016\/0042-6822(81)90075-1","article-title":"Identification of the antiviral factor in culture medium of mosquito cells persistently infected with Banzi virus","volume":"110","author":"Lee","year":"1981","journal-title":"Virology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/0042-6822(86)90262-X","article-title":"Persistent infection of Aedes albopictus C6\/36 cells by Bunyamwera virus","volume":"150","author":"Elliott","year":"1986","journal-title":"Virology"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2199","DOI":"10.1099\/0022-1317-69-9-2199","article-title":"Phenotypes of St Louis Encephalitis Virus Mutants Produced in Persistently Infected Mosquito Cell Cultures","volume":"69","author":"Randolph","year":"1988","journal-title":"J. Gen. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1099\/0022-1317-73-1-53","article-title":"Defective RNAs in mosquito cells persistently infected with Bunyamwera virus","volume":"73","author":"Scallan","year":"1992","journal-title":"J. Gen. Virol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1703","DOI":"10.1099\/0022-1317-74-8-1703","article-title":"St Louis encephalitis virus establishes a productive, cytopathic and persistent infection of Sf9 cells","volume":"74","author":"Zhang","year":"1993","journal-title":"J. Gen. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1159\/000150352","article-title":"Phenotypic Characteristics of Dengue 2 Virus Persistently Infected in a C6\/36 Clone of Aedes albopictus Cells","volume":"37","author":"Chen","year":"1994","journal-title":"Intervirology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1016\/j.virusres.2006.10.013","article-title":"Defective interfering RNAs of Japanese encephalitis virus found in mosquito cells and correlation with persistent infection","volume":"124","author":"Tsai","year":"2007","journal-title":"Virus Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.dci.2008.02.008","article-title":"Mosquito cells accommodate balanced, persistent co-infections with a densovirus and Dengue virus","volume":"32","author":"Kanthong","year":"2008","journal-title":"Dev. Comp. Immunol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"583","DOI":"10.1007\/s00705-012-1525-2","article-title":"Detection and sequencing of defective viral genomes in C6\/36 cells persistently infected with dengue virus 2","volume":"158","year":"2013","journal-title":"Arch. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1099\/0022-1317-40-3-531","article-title":"Isolation of a Singh\u2019s Aedes albopictus Cell Clone Sensitive to Dengue and Chikungunya Viruses","volume":"40","author":"Igarashi","year":"1978","journal-title":"J. Gen. Virol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1007\/BF02626177","article-title":"Maintaining mosquito cell lines at high tempetatures: Effects on the replication of flaviviruses","volume":"25","author":"Kuno","year":"1989","journal-title":"In Vitro Cell. Dev. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1128\/jcm.22.2.250-254.1985","article-title":"Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: Comparison of the BHK suspension test with standard plaque reduction neutralization","volume":"22","author":"Morens","year":"1985","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","first-page":"71","article-title":"Neutralizaci\u00f3n por reducci\u00f3n de placas como m\u00e9todo espec\u00edfico para el diagn\u00f3stico serol\u00f3gico de Fiebre Amarilla","volume":"18","author":"Castillo","year":"2001","journal-title":"Rev. Peru. Med. Exp. Salud P\u00fablica"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1007\/s00705-012-1596-0","article-title":"Surface proteins of C6\/36 cells involved in dengue virus 4 binding and entry","volume":"158","year":"2013","journal-title":"Arch. Virol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Scott, J.C., Brackney, D.E., Campbell, C.L., Bondu-Hawkins, V., Hjelle, B., Ebel, G.D., Olson, K.E., and Blair, C.D. (2010). Comparison of Dengue Virus Type 2-Specific Small RNAs from RNA Interference-Competent and \u2013Incompetent Mosquito Cells. PLoS Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000848"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.virol.2019.02.018","article-title":"Mosquito cells persistently infected with dengue virus produce viral particles with host-dependent replication","volume":"531","author":"Jaworski","year":"2019","journal-title":"Virology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"76","DOI":"10.4269\/ajtmh.2002.67.76","article-title":"A putative receptor for dengue virus in mosquito tissues: Localization of a 45-kDa glycoprotein","volume":"67","year":"2002","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Brackney, D.E., Scott, J.C., Sagawa, F., Woodward, J.E., Miller, N.A., Schilkey, F.D., Mudge, J., Wilusz, J., Olson, K.E., and Blair, C.D. (2010). C6\/36 Aedes albopictus Cells Have a Dysfunctional Antiviral RNA Interference Response. PLoS Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000856"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"6545","DOI":"10.1093\/nar\/gkv590","article-title":"Distinct sets of PIWI proteins produce arbovirus and transposon-derived piRNAs in Aedes aegypti mosquito cells","volume":"43","author":"Miesen","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1099\/0022-1317-72-3-573","article-title":"Persistent infection of Vero cells by the flavivirus Murray Valley encephalitis virus","volume":"72","author":"Poidinger","year":"1991","journal-title":"J. Gen. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1128\/jvi.24.2.580-589.1977","article-title":"Persistent infection of cultured mammalian cells by Japanese encephalitis virus","volume":"24","author":"Schmaljohn","year":"1977","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1016\/s1016-8478(23)12908-6","article-title":"Characterization of homologous defective interfering RNA during persistent infection of Vero cells with Japanese encephalitis virus","volume":"21","author":"Yoon","year":"2006","journal-title":"Mol. Cells"},{"key":"ref_35","first-page":"1324","article-title":"Characterization and E protein expression of mutant strains during persistent infection of KN73 cells with Japanese encephalitis virus","volume":"115","author":"Feng","year":"2002","journal-title":"Chin. Med. J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1016\/0042-6822(83)90373-2","article-title":"Interference between bunyaviruses in Aedes triseriatus mosquitoes","volume":"127","author":"Beaty","year":"1983","journal-title":"Virology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1128\/jvi.29.1.51-60.1979","article-title":"Novel antiviral activity found in the media of Sindbis virus-persistently infected mosquito (Aedes albopictus) cell cultures","volume":"29","author":"Riedel","year":"1979","journal-title":"J. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"652","DOI":"10.1128\/jvi.47.3.652-655.1983","article-title":"Antiviral activity released from Aedes albopictus cells persistently infected with Semliki forest virus","volume":"47","author":"Dalgarno","year":"1983","journal-title":"J. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1006\/viro.1993.1233","article-title":"Purification and Characterization of a Sindbis Virus-Induced Peptide Which Stimulates Its Own Production and Blocks Virus RNA Synthesis","volume":"194","author":"Luo","year":"1993","journal-title":"Virology"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1006\/viro.1994.1178","article-title":"A 55-kDa Protein Induced in Aedes albopictus (Mosquito) Cells by Antiviral Protein","volume":"200","author":"Luo","year":"1994","journal-title":"Virology"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/ni.2542","article-title":"RNA-mediated interference and reverse transcription control the persistence of RNA viruses in the insect model Drosophila","volume":"14","author":"Goic","year":"2013","journal-title":"Nat. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"12410","DOI":"10.1038\/ncomms12410","article-title":"Virus-derived DNA drives mosquito vector tolerance to arboviral infection","volume":"7","author":"Goic","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"e2213701120","DOI":"10.1073\/pnas.2213701120","article-title":"RNA interference is essential to modulating the pathogenesis of mosquito-borne viruses in the yellow fever mosquito Aedes aegypti","volume":"120","author":"Samuel","year":"2023","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"G\u00f6ertz, G.P., Miesen, P., Overheul, G.J., van Rij, R.P., van Oers, M.M., and Pijlman, G.P. (2019). Mosquito Small RNA Responses to West Nile and Insect-Specific Virus Infections in Aedes and Culex Mosquito Cells. Viruses, 11.","DOI":"10.3390\/v11030271"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Morazzani, E.M., Wiley, M.R., Murreddu, M.G., Adelman, Z.N., and Myles, K.M. (2012). Production of Virus-Derived Ping-Pong-Dependent piRNA-like Small RNAs in the Mosquito Soma. PLoS Pathog., 8.","DOI":"10.1371\/journal.ppat.1002470"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3511","DOI":"10.1016\/j.cub.2017.09.067","article-title":"The Diversity, Structure, and Function of Heritable Adaptive Immunity Sequences in the Aedes aegypti Genome","volume":"27","author":"Whitfield","year":"2017","journal-title":"Curr. Biol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1551","DOI":"10.1099\/jgv.0.001157","article-title":"The antiviral piRNA response in mosquitoes?","volume":"99","author":"Varjak","year":"2018","journal-title":"J. Gen. Virol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1493","DOI":"10.1534\/g3.113.006742","article-title":"The Developmental Transcriptome of the Mosquito Aedes aegypti, an Invasive Species and Major Arbovirus Vector","volume":"3","author":"Akbari","year":"2013","journal-title":"G3"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1114","DOI":"10.3389\/fgene.2019.01114","article-title":"Deducing the Role of Virus Genome-Derived PIWI-Associated RNAs in the Mosquito\u2013Arbovirus Arms Race","volume":"10","author":"Blair","year":"2019","journal-title":"Front. Genet."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.bbrc.2007.07.179","article-title":"Identification and characterization of Piwi subfamily in insects","volume":"362","author":"Zhou","year":"2007","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_51","first-page":"4881","article-title":"Histone-derived piRNA biogenesis depends on the ping-pong partners Piwi5 and Ago3 in Aedes aegypti","volume":"45","author":"Girardi","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Wang, Y., Jin, B., Liu, P., Li, J., Chen, X., and Gu, J. (2018). piRNA Profiling of Dengue Virus Type 2-Infected Asian Tiger Mosquito and Midgut Tissues. Viruses, 10.","DOI":"10.3390\/v10040213"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Marconcini, M., Hernandez, L., Iovino, G., Hou\u00e9, V., Valerio, F., Palatini, U., Pischedda, E., Crawford, J.E., White, B.J., and Lin, T. (2019). Polymorphism analyses and protein modelling inform on functional specialization of Piwi clade genes in the arboviral vector Aedes albopictus. PLoS Negl. Trop. Dis., 13.","DOI":"10.1101\/677062"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Miesen, P., Ivens, A., Buck, A.H., and van Rij, R.P. (2016). Small RNA Profiling in Dengue Virus 2-Infected Aedes Mosquito Cells Reveals Viral piRNAs and Novel Host miRNAs. PLoS Negl. Trop. Dis., 10.","DOI":"10.1371\/journal.pntd.0004452"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1016\/j.ibmb.2019.04.008","article-title":"Small RNA responses of Culex mosquitoes and cell lines during acute and persistent virus infection","volume":"109","author":"Prasad","year":"2019","journal-title":"Insect Biochem. Mol. Biol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Varjak, M., Donald, C.L., Mottram, T.J., Sreenu, V.B., Merits, A., Maringer, K., Schnettler, E., and Kohl, A. (2017). Characterization of the Zika virus induced small RNA response in Aedes aegypti cells. PLoS Negl. Trop. Dis., 11.","DOI":"10.1371\/journal.pntd.0006010"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e00144-17","DOI":"10.1128\/mSphere.00144-17","article-title":"Aedes aegypti Piwi4 Is a Noncanonical PIWI Protein Involved in Antiviral Responses","volume":"2","author":"Varjak","year":"2017","journal-title":"mSphere"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Marconcini, M., Pischedda, E., Hou\u00e9, V., Palatini, U., Lozada-Ch\u00e1vez, N., Sogliani, D., Failloux, A.-B., and Bonizzoni, M. (2021). Profile of Small RNAs, vDNA Forms and Viral Integrations in Late Chikungunya Virus Infection of Aedes albopictus Mosquitoes. Viruses, 13.","DOI":"10.3390\/v13040553"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Hess, A.M., Prasad, A.N., Ptitsyn, A., Ebel, G.D., E Olson, K., Barbacioru, C., Monighetti, C., and Campbell, C.L. (2011). Small RNA profiling of Dengue virus-mosquito interactions implicates the PIWI RNA pathway in anti-viral defense. BMC Microbiol., 11.","DOI":"10.1186\/1471-2180-11-45"},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Sinclair, J.B., and Asgari, S. (2020). Ross River Virus Provokes Differentially Expressed MicroRNA and RNA Interference Responses in Aedes aegypti Mosquitoes. Viruses, 12.","DOI":"10.3390\/v12070695"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Walsh, E., Torres, T.Z.B., and R\u00fcckert, C. (2022). Culex Mosquito Piwi4 Is Antiviral against Two Negative-Sense RNA Viruses. Viruses, 14.","DOI":"10.3390\/v14122758"},{"key":"ref_62","doi-asserted-by":"crossref","unstructured":"Yen, P.-S., Chen, C.-H., Sreenu, V., Kohl, A., and Failloux, A.-B. (2019). Assessing the Potential Interactions between Cellular miRNA and Arboviral Genomic RNA in the Yellow Fever Mosquito, Aedes aegypti. Viruses, 11.","DOI":"10.3390\/v11060540"},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Eng, M.W., Clemons, A., Hill, C., Engel, R., Severson, D.W., and Behura, S.K. (2018). Multifaceted functional implications of an endogenously expressed tRNA fragment in the vector mosquito Aedes aegypti. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006186"},{"key":"ref_64","doi-asserted-by":"crossref","unstructured":"Kumar, V., Garg, S., Gupta, L., Gupta, K., Diagne, C.T., Miss\u00e9, D., Pompon, J., Kumar, S., and Saxena, V. (2021). Delineating the Role of Aedes aegypti ABC Transporter Gene Family during Mosquito Development and Arboviral Infection via Transcriptome Analyses. Pathogens, 10.","DOI":"10.3390\/pathogens10091127"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.actatropica.2016.02.028","article-title":"Characterization and expression analysis of gene encoding heme peroxidase HPX15 in major Indian malaria vector Anopheles stephensi (Diptera: Culicidae)","volume":"158","author":"Kajla","year":"2016","journal-title":"Acta Trop."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/9\/1135\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T20:45:45Z","timestamp":1760129145000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/9\/1135"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,6]]},"references-count":65,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2023,9]]}},"alternative-id":["pathogens12091135"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12091135","relation":{},"ISSN":["2076-0817"],"issn-type":[{"type":"electronic","value":"2076-0817"}],"subject":[],"published":{"date-parts":[[2023,9,6]]}}}