{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T02:34:14Z","timestamp":1760150054699,"version":"build-2065373602"},"reference-count":44,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T00:00:00Z","timestamp":1696204800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Rabies Diagnostic Project","award":["P10000045","871029"],"award-info":[{"award-number":["P10000045","871029"]}]},{"name":"European Virus Archive global (EVAg)","award":["P10000045","871029"],"award-info":[{"award-number":["P10000045","871029"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Rabies is one of the most significant public and veterinary health problems, causing approximately 59,000 human deaths annually in the developing countries of Asia and Africa. The aetiologic agent, a viral species of the Lyssavirus genus, is highly neurotropic and has a wide host range, including terrestrial mammals and several Chiropteran species. The Lyssavirus mokola (MOKV) was first isolated in the late 1960s from organ pools of shrews (Crocidura flavescens manni) in the Mokola forest (Nigeria). To date, at least 30 MOKV isolations have been confirmed, all exclusively from Africa, with 73% from southern Africa. There is limited knowledge about the epidemiology of MOKV, and the reservoir host species is unknown. Here, we report on the molecular characterization of rabies viruses originating from both domestic and African wild cats. A partial region of the lyssavirus genome, encoding the nucleoprotein, was amplified and sequenced. Nucleotide sequence analysis demonstrated that 98% of cats were infected with both the canid and mongoose rabies virus variants, as well as a rare lyssavirus, Lyssavirus mokola, from a domestic cat from Eswatini. Furthermore, the nucleotide sequence divergence between the recently identified MOKV isolate and the historical Lyssavirus mokola isolates ranged from 6.8% to 8.3%. This study further highlights the association between the potential host species of Lyssavirus mokola and the domestic cat as an incidental host, and the important role cats may play in rabies transmission dynamics in the country. Therefore, continuous vaccination of domestic cats against rabies is crucial, even after the elimination of dog-mediated rabies, as spillover related to sylvatic rabies cycles is likely to occur.<\/jats:p>","DOI":"10.3390\/pathogens12101212","type":"journal-article","created":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T04:17:19Z","timestamp":1696220239000},"page":"1212","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Molecular Characterization of Lyssaviruses Originating from Domestic and Wild Cats Provides an Insight on the Diversity of Lyssaviruses and a Risk of Rabies Transmission to Other Susceptible Mammals and Humans in South Africa"],"prefix":"10.3390","volume":"12","author":[{"given":"Kefentse","family":"Tsie","sequence":"first","affiliation":[{"name":"WOAH Rabies Reference Laboratory, Agricultural Research Council (Onderstepoort Veterinary Research), Onderstepoort, Pretoria 0110, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3272-9547","authenticated-orcid":false,"given":"Ernest","family":"Ngoepe","sequence":"additional","affiliation":[{"name":"WOAH Rabies Reference Laboratory, Agricultural Research Council (Onderstepoort Veterinary Research), Onderstepoort, Pretoria 0110, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Baby","family":"Phahladira","sequence":"additional","affiliation":[{"name":"WOAH Rabies Reference Laboratory, Agricultural Research Council (Onderstepoort Veterinary Research), Onderstepoort, Pretoria 0110, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nelisiwe","family":"Khumalo","sequence":"additional","affiliation":[{"name":"Central Veterinary Laboratory, Corner Althea Road and Central, Manzini H100, Eswatini"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7842-7985","authenticated-orcid":false,"given":"Claude","family":"Sabeta","sequence":"additional","affiliation":[{"name":"WOAH Rabies Reference Laboratory, Agricultural Research Council (Onderstepoort Veterinary Research), Onderstepoort, Pretoria 0110, South Africa"},{"name":"Department of Veterinary Tropical Diseases, University of Pretoria, Private Bag 04, Onderstepoort, Pretoria 0110, South Africa"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Hampson, K., Coudeville, L., Lembo, T., Sambo, M., Kieffer, A., Attlan, M., Barrat, J., Blanton, J.D., Briggs, D.J., and Cleaveland, S. (2015). Estimating the global burden of endemic canine rabies. PLOS Neglected Trop. Dis., 9.","DOI":"10.1371\/journal.pntd.0003786"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2429","DOI":"10.1007\/s00705-022-05516-5","article-title":"Recent changes to virus taxonomy ratified by the International Committee on Taxonomy of Viruses (2022)","volume":"167","author":"Walker","year":"2022","journal-title":"Arch. Virol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1111\/tbed.12833","article-title":"Tentative novel lyssavirus in a bat in Finland","volume":"65","author":"Nokireki","year":"2018","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"3056","DOI":"10.3201\/eid2612.203592","article-title":"Lyssaviruses in insectivorous bats, South Africa, 2003\u20132018","volume":"26","author":"Coertse","year":"2020","journal-title":"Emerg. Infect. Dis."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"8096","DOI":"10.1128\/JVI.75.17.8096-8104.2001","article-title":"Host switching in the Lyssavirus history from the Chiroptera to Carnivora orders","volume":"17","author":"Badrane","year":"2001","journal-title":"J. Virol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"664","DOI":"10.3201\/eid1804.111553","article-title":"Ikoma lyssavirus, highly divergent novel lyssavirus in an African civet","volume":"18","author":"Marston","year":"2012","journal-title":"Emerg. Infect. Dis."},{"key":"ref_7","unstructured":"World Health Organization (2023, July 19). Neglected Tropical Diseases: Rabies. Available online: https:\/\/www.who.int\/data\/gho\/data\/themes\/topics\/rabies."},{"key":"ref_8","first-page":"295","article-title":"Canid and viverrid rabies viruses in South Africa","volume":"60","author":"King","year":"1993","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1016\/j.virusres.2004.12.003","article-title":"Mongoose rabies in southern Africa: A re-evaluation based on molecular epidemiology","volume":"109","author":"Nel","year":"2005","journal-title":"Virus Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1099\/0022-1317-76-1-73","article-title":"Molecular epidemiology of rabies virus in South Africa: Evidence for two distinct virus groups","volume":"76","author":"Thomson","year":"1995","journal-title":"J. Gen. Virol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.virusres.2010.02.018","article-title":"Evolutionary history of African mongoose rabies","volume":"150","author":"Markotter","year":"2010","journal-title":"Virus Res."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/j.virusres.2009.02.012","article-title":"The spread of canine rabies into Free State province of South Africa: A molecular epidemiological characterization","volume":"142","author":"Ngoepe","year":"2009","journal-title":"Virus Res."},{"key":"ref_13","first-page":"1","article-title":"Rabies of canid biotype in wild dog (Lycaon pictus) and spotted hyaena (Crocuta crocuta) in Madikwe Game Reserve, South Africa in 2014-2015: Diagnosis, possible origins, and implications for control","volume":"89","author":"Sabeta","year":"2018","journal-title":"J. South Afr. Vet. Assoc."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"356","DOI":"10.4269\/ajtmh.1972.21.356","article-title":"Mokola virus: Further studies on IbAn 27377, a new rabies-related etiological agent of zoonosis in Nigeria","volume":"21","author":"Kemp","year":"1972","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1128\/jvi.6.5.690-692.1970","article-title":"Two African viruses serologically and morphologically related to rabies virus","volume":"6","author":"Shope","year":"1970","journal-title":"J. Virol."},{"key":"ref_16","first-page":"93","article-title":"Isolation of a rabies-related virus from the cerebrospinal fluid of a child with aseptic meningitis","volume":"3","author":"Familusi","year":"1972","journal-title":"Afr. J. Med. Med. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"959","DOI":"10.4269\/ajtmh.1972.21.959","article-title":"A fatal human infection with Mokola virus","volume":"21","author":"Familusi","year":"1972","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_18","first-page":"245","article-title":"Isolation of a strain of Mokola virus in Cameroon","volume":"129","author":"Rickenbach","year":"1978","journal-title":"Ann. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1016\/S0769-2617(84)80039-8","article-title":"Premier isolment du virus Mokola a partir D\u2019un rongeur (Lophuromys sikapusi)","volume":"135","author":"Saluzzo","year":"1984","journal-title":"Ann. Inst Pasteur Virol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"972","DOI":"10.1093\/infdis\/166.5.972","article-title":"Isolation and characterization of 115 street rabies virus isolates from Ethiopia by using monoclonal antibodies: Identification of 2 isolates of Mokola and Lagos Bat viruses","volume":"166","author":"Mebatsion","year":"1992","journal-title":"J. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"338","DOI":"10.1136\/vr.110.14.338","article-title":"Atypical rabies virus in cats and a dog in Zimbabwe","volume":"110","author":"Foggin","year":"1982","journal-title":"Vet Rec."},{"key":"ref_22","unstructured":"Foggin, C.M. (1988). Rabies and Rabies-Related Viruses in Zimbabwe: Historical, Virological, and Ecological Aspects. [Ph.D. Thesis, University of Zimbabwe]."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"92","DOI":"10.4102\/jsava.v72i2.624","article-title":"Report of isolations of unusual lyssaviruses (rabies and Mokola virus) identified retrospectively from Zimbabwe","volume":"72","author":"Bingham","year":"2001","journal-title":"J. South Afr. Vet. Assoc."},{"key":"ref_24","first-page":"119","article-title":"Further isolation of Mokola virus in South Africa","volume":"138","author":"Meredith","year":"1996","journal-title":"Vet. Rec."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12917-017-0948-0","article-title":"New isolations of the rabies related Mokola virus from South Africa","volume":"13","author":"Coertse","year":"2016","journal-title":"BMC Vet. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1023\/A:1008120511752","article-title":"New cases of Mokola virus in South Africa","volume":"20","author":"Nel","year":"2000","journal-title":"Virus Genes"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1371","DOI":"10.3201\/eid1309.070466","article-title":"Mokola virus in domestic mammals, South Africa","volume":"13","author":"Sabeta","year":"2007","journal-title":"Emerg. Infect. Dis."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1111\/j.1574-695X.2009.00609.x","article-title":"Mokola virus involved in a human contact (South Africa)","volume":"58","author":"Sabeta","year":"2010","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"169","DOI":"10.4102\/jsava.v69i4.847","article-title":"Mokola virus infection: Description of recent South African cases and a review of the virus epidemiology","volume":"69","author":"Bosch","year":"1998","journal-title":"South Afr. Vet. Assoc."},{"key":"ref_30","unstructured":"Meslin, F.C., Kaplan, M.M., and Koprowski, H. (1996). Laboratory Techniques in Rabies, World Health Organization. [4th ed.]."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1913","DOI":"10.3201\/eid1212.060514","article-title":"Isolation of Lagos bat virus from water mongoose","volume":"12","author":"Markotter","year":"2006","journal-title":"Emerg. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA11: Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_33","first-page":"406","article-title":"The Neighbor-Joining Method: A New Method for Reconstructing Phylogenetic Trees","volume":"4","author":"Saitou","year":"1987","journal-title":"Mol. Biol. Evol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"182","DOI":"10.1093\/sysbio\/42.2.182","article-title":"An empirical test of bootstrapping as a method for assessing confidence in phylogenetic analysis","volume":"42","author":"Hillis","year":"1993","journal-title":"Syst. Biol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1210203","DOI":"10.3389\/fpubh.2023.1210203","article-title":"Cat rabies in Brazil: A growing One Health concern","volume":"11","author":"Mori","year":"2023","journal-title":"Front. Public Health"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Kgaladi, J., Wright, N., Coertse, J., Markotter, W., Marston, D., Fooks, A.R., Freuling, C.M., M\u00fcller, T.F., Sabeta, C.T., and Nel, L.H. (2013). Diversity and epidemiology of Mokola virus. PloS Neglected Trop. Dis., 7.","DOI":"10.1371\/journal.pntd.0002511"},{"key":"ref_37","first-page":"a2036","article-title":"Human rabies associated with domestic cat exposures in South Africa, 1983\u20132018","volume":"91","author":"Blumberg","year":"2020","journal-title":"J. South Afr. Vet. Assoc."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"195","DOI":"10.2460\/javma.256.2.195","article-title":"Public Veterinary Medicine: Public Health: Rabies surveillance in the United States during 2018","volume":"256","author":"Ma","year":"2020","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Brunt, S., Solomon, H., Brown, K., and Davis, A. (2021). Feline and Canine Rabies in New York State, USA. Viruses, 13.","DOI":"10.3390\/v13030450"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1111","DOI":"10.2460\/javma.245.10.1111","article-title":"Rabies surveillance in the United States during 2013","volume":"245","author":"Dyer","year":"2014","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4102\/ojvr.v88i1.1911","article-title":"Surveillance of the rabies-related lyssavirus, Mokola in non-volant small mammals in South Africa","volume":"88","author":"McMahon","year":"2021","journal-title":"Onderstepoort J. Vet. Res."},{"key":"ref_42","unstructured":"Bernstein, P.L. (2007). The Welfare of Cats, Springer."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Coertse, J., Viljoen, N., Weyer, J., and Markotter, W. (2023). Comparative Neutralization Activity of Commercial Rabies Immunoglobulin against Diverse Lyssaviruses. Vaccines, 11.","DOI":"10.3390\/vaccines11071255"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"(2018). World Health Organization Rabies Vaccines: WHO Position Paper, April 2018\u2014Recommendations. Vaccine, 36, 5500\u20135503.","DOI":"10.1016\/j.vaccine.2018.06.061"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/10\/1212\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T21:03:58Z","timestamp":1760130238000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/12\/10\/1212"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,2]]},"references-count":44,"journal-issue":{"issue":"10","published-online":{"date-parts":[[2023,10]]}},"alternative-id":["pathogens12101212"],"URL":"https:\/\/doi.org\/10.3390\/pathogens12101212","relation":{},"ISSN":["2076-0817"],"issn-type":[{"type":"electronic","value":"2076-0817"}],"subject":[],"published":{"date-parts":[[2023,10,2]]}}}