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The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatu\u00ed, S\u00e3o Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86\/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders.<\/jats:p>","DOI":"10.3390\/pathogens12101250","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T08:08:33Z","timestamp":1697530113000},"page":"1250","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing"],"prefix":"10.3390","volume":"12","author":[{"given":"Paula Adas Pereira","family":"Suniga","sequence":"first","affiliation":[{"name":"Postgraduate Program in Animal Science, Faculty of Veterinary Medicine and Animal Science-FAMEZ\/UFMS, Federal University of Mato Grosso Do Sul, Av. Senador Filinto Muller, 2443, Campo Grande 79074-460, MS, Brazil"},{"name":"MAI\/DAI Scholarship, Federal University of Mato Grosso Do Sul, Cidade Universit\u00e1ria, Av. Costa E Silva, Campo Grande 79070-900, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cynthia","family":"Mantovani","sequence":"additional","affiliation":[{"name":"Embrapa Beef Cattle\/Ministry of Agriculture and Livestock Scholarship, Embrapa Beef Cattle, Av. R\u00e1dio Maia, 830, Campo Grande 79106-550, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Goretti","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Embrapa Beef Cattle, Campo Grande 79106-550, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1690-4341","authenticated-orcid":false,"given":"Andr\u00e9a Alves","family":"do Egito","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Animal Science, Faculty of Veterinary Medicine and Animal Science-FAMEZ\/UFMS, Federal University of Mato Grosso Do Sul, Av. Senador Filinto Muller, 2443, Campo Grande 79074-460, MS, Brazil"},{"name":"Embrapa Beef Cattle, Campo Grande 79106-550, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9817-7223","authenticated-orcid":false,"given":"Newton Val\u00e9rio","family":"Verbisck","sequence":"additional","affiliation":[{"name":"Embrapa Beef Cattle, Campo Grande 79106-550, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lenita Ramires","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Embrapa Beef Cattle, Campo Grande 79106-550, MS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto Mart\u00edn Rivera","family":"D\u00e1vila","sequence":"additional","affiliation":[{"name":"Computational and Systems Biology Laboratory, Graduate Program in Biodiversity and Health, Oswaldo Cruz Institute, Fiocruz, Rio de Janeiro 21040-900, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3485-2434","authenticated-orcid":false,"given":"Cristina Kraemer","family":"Zimpel","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Michigan State University, East Lansing, MI 48824, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Carolina Sisco","family":"Zerpa","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Research in Mycobacteria, Department of Microbiology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8178-6604","authenticated-orcid":false,"given":"Daniela Pontes","family":"Chiebao","sequence":"additional","affiliation":[{"name":"Animal Health Research Center, Biological Institute, Av. Conselheiro Rodrigues Alves, 1252, S\u00e3o Paulo 04014-002, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana M\u00e1rcia","family":"de S\u00e1 Guimar\u00e3es","sequence":"additional","affiliation":[{"name":"Laboratory of Applied Research in Mycobacteria, Department of Microbiology, Institute of Biomedical Sciences, University of S\u00e3o Paulo, S\u00e3o Paulo 05508-000, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra Figueiredo","family":"de Castro Nassar","sequence":"additional","affiliation":[{"name":"Animal Health Research Center, Biological Institute, Av. 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