{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T04:09:01Z","timestamp":1790741341797,"version":"4.1.0"},"reference-count":31,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s","award":["202110267000287"],"award-info":[{"award-number":["202110267000287"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Goi\u00e1s","award":["317557\/2021-1"],"award-info":[{"award-number":["317557\/2021-1"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brazil (CAPES)\u2014","award":["202110267000287"],"award-info":[{"award-number":["202110267000287"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brazil (CAPES)\u2014","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior\u2014Brazil (CAPES)\u2014","award":["317557\/2021-1"],"award-info":[{"award-number":["317557\/2021-1"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["202110267000287"],"award-info":[{"award-number":["202110267000287"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","award":["317557\/2021-1"],"award-info":[{"award-number":["317557\/2021-1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Amblyomma sculptum is a relevant tick species from a One Health perspective, playing an important role as a vector of Rickettsia rickettsii, the main agent of spotted fever rickettsiosis in Brazil. In this study, we evaluated the susceptibility of two A. sculptum populations from Goi\u00e1s state (midwestern Brazil) to different acaricides. The first tick population (GYN strain) originated from an experimental farm, where the ticks are annually exposed to acaricides. The second (PNE strain) was collected in a national park (Emas National Park), where the ticks had not been exposed to acaricides. Immersion tests were conducted with 21-day-old laboratory-reared larvae and nymphs originating from adult ticks collected in the areas mentioned above. The chosen acaricides were two synthetic pyrethroids (cypermethrin and deltamethrin), one organophosphate (chlorfenvinphos), one formamidine (amitraz), and two combinations of pyrethroids and organophosphates (cypermethrin, chlorpyrifos and citronellal; cypermethrin, fenthion and chlorpyrifos). Mortality data were used to determine the lethal concentration (LC) values at which 50%, 90%, and 99% of the ticks died (LC50, LC90, and LC99, respectively), and resistance ratios (RR) were calculated based on the LC values. The RR revealed differences between the acaricide-exposed (GYN) and unexposed (PNE) tick strains. The PNE strain larvae and nymphs were susceptible to all the tested acaricides. The GYN strain larvae were tolerant to cypermethrin, whereas the nymphs were tolerant to deltamethrin, chlorfenvinphos, and the combination of cypermethrin, chlorpyrifos, and citronellal (2 &lt; RR \u2264 10). The GYN strain nymphs were resistant to amitraz (RR &gt; 10). This is the first report of A. sculptum nymphs with resistance to amitraz and tolerance to deltamethrin, chlorfenvinphos, and the combination of cypermethrin, chlorpyrifos, and citronellal.<\/jats:p>","DOI":"10.3390\/pathogens12111304","type":"journal-article","created":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T11:16:27Z","timestamp":1698750987000},"page":"1304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Susceptibility of Amblyomma sculptum, Vector of Rickettsia rickettsii, Ticks from a National Park and an Experimental Farm to Different Synthetic Acaricides"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3810-334X","authenticated-orcid":false,"given":"Ennya Rafaella Neves","family":"Cardoso","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephani F\u00e9lix","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah Alves","family":"Dias","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rayane Almeida","family":"Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana Avelar","family":"Tavares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7284-5801","authenticated-orcid":false,"given":"Lucianne Cardoso","family":"Neves","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Warley Vieira de Freitas","family":"Paula","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5560-0925","authenticated-orcid":false,"given":"Gracielle Teles","family":"P\u00e1dua","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas Jalowitzki","family":"de Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0119-0435","authenticated-orcid":false,"given":"Raquel Loren dos Reis","family":"Paludo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2992-9636","authenticated-orcid":false,"given":"Isabela Santos","family":"Silva","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia, Ecologia e Controle de Carrapatos\u2014LABEC, Centro de Parasitologia, Instituto de Patologia Tropical e Sa\u00fade P\u00fablica, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphaela Bueno Mendes","family":"Bittencourt","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel C\u00e2ndido","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e 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Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1496-6274","authenticated-orcid":false,"given":"Filipe","family":"Dantas-Torres","sequence":"additional","affiliation":[{"name":"Laboratory of Immunoparasitology, Department of Immunology, Aggeu Magalh\u00e3es Institute, Oswaldo Cruz Foundation (FIOCRUZ), Recife 50740-465, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caio Marcio De Oliveira","family":"Monteiro","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia, Ecologia e Controle de Carrapatos\u2014LABEC, Centro de Parasitologia, Instituto de Patologia Tropical e Sa\u00fade P\u00fablica, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3817-225X","authenticated-orcid":false,"given":"Felipe da Silva","family":"Krawczak","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Doen\u00e7as Parasit\u00e1rias\u2014LADOPAR, Setor de Medicina Veterin\u00e1ria Preventiva, Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s\u2014UFG, Goi\u00e2nia 74690-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,10,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1007\/s10493-006-9027-0","article-title":"Ticks (Ixodidae) on Humans in South America","volume":"40","author":"Guglielmone","year":"2006","journal-title":"Exp. 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