{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T23:22:52Z","timestamp":1769642572166,"version":"3.49.0"},"reference-count":44,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Henan Province modern agricultural industrial technology system","award":["HARS-22-15-G1"],"award-info":[{"award-number":["HARS-22-15-G1"]}]},{"name":"Henan Province modern agricultural industrial technology system","award":["2022020103"],"award-info":[{"award-number":["2022020103"]}]},{"name":"Agriculture in Henan Province for fine breed research project","award":["HARS-22-15-G1"],"award-info":[{"award-number":["HARS-22-15-G1"]}]},{"name":"Agriculture in Henan Province for fine breed research project","award":["2022020103"],"award-info":[{"award-number":["2022020103"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Toxoplasma gondii is a widespread protozoan parasite that infects most warm-blooded animals, and felids can serve as intermediate and definitive hosts. Pathological diagnosis and serological and etiological investigations were conducted on a captive caracal (Caracal caracal) carcass collected from China in 2022. Pathological diagnosis revealed that cardiac insufficiency, pulmonary edema, hepatic failure, and renal insufficiency were the causes of the caracal\u2019s death. A modified agglutination test (cut-off: 1:25) revealed that IgG antibodies against T. gondii were present in the myocardium juice (1:1600), ascitic fluid (1:3200), and hydropericardium (1:800). A viable T. gondii (TgCaracalCHn2) strain was isolated from the tissue samples (heart, brain, spleen, and skeletal muscle) of this caracal using a mouse bioassay. The genotype of TgCaracalCHn2 was ToxoDB#5 (Type II variant), as determined via RFLP-PCR. The strain was avirulent in Swiss mice and matched the prediction of ROP18 and ROP5 gene alleles of TgCaracalCHn2 (2\/2). Mild tissue cysts (203 \u00b1 265) were observed in mice brains after inoculation with TgCaracalCHn2 tachyzoites. ToxoDB#5 is the dominant genotype in North American wildlife, and this is the first documented isolation of T. gondii ToxoDB#5 from China. This indicates that caracal plays an important role in the transmission of this T. gondii genotype.<\/jats:p>","DOI":"10.3390\/pathogens12121412","type":"journal-article","created":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T07:44:42Z","timestamp":1701330282000},"page":"1412","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Isolation and Characterization of a Viable Toxoplasma gondii from Captive Caracal (Caracal caracal)"],"prefix":"10.3390","volume":"12","author":[{"given":"Hongjie","family":"Ren","sequence":"first","affiliation":[{"name":"College of Animal Science, Henan Agricultural University, Zhengzhou 450000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gaohui","family":"Mao","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Henan Agricultural University, Zhengzhou 450000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niuping","family":"Zhu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qunchao","family":"Liang","sequence":"additional","affiliation":[{"name":"Henan Yinji Jiabao Amusement Park Management Co., Ltd., Zhengzhou 452300, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibao","family":"Jiang","sequence":"additional","affiliation":[{"name":"College of Animal Science, Henan Agricultural University, Zhengzhou 450000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yurong","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Henan Agricultural University, Zhengzhou 450000, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Dubey, J.P. 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