{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T23:37:38Z","timestamp":1790725058338,"version":"4.1.0"},"reference-count":26,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T00:00:00Z","timestamp":1701302400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Aix-Marseille-University"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Laryngeal tuberculosis is a rare form of extrapulmonary tuberculosis that questions the natural history of this infection. We report one such case in which a pathological examination of a laryngeal biopsy revealed granulomatous inflammation with caseous necrosis. Further investigations combining immunofluorescence detection of macrophages and in situ hybridization of Mycobacterium tuberculosis indicated the presence of Mycobacterium tuberculosis (M. tuberculosis) in laryngeal granulomatous inflammatory lesions. This observation suggests that the natural history of laryngeal tuberculosis does not differ from that of other forms, guiding early diagnosis in patients with laryngeal lesions to ensure appropriate check-ups and treatment.<\/jats:p>","DOI":"10.3390\/pathogens12121413","type":"journal-article","created":{"date-parts":[[2023,11,30]],"date-time":"2023-11-30T09:39:12Z","timestamp":1701337152000},"page":"1413","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Mycobacterium tuberculosis Resides in Macrophages in Laryngeal Tuberculosis: A Case Report"],"prefix":"10.3390","volume":"12","author":[{"given":"Wafaa","family":"Achache","sequence":"first","affiliation":[{"name":"MEPHI, Aix-Marseille University, IRD, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9394-3977","authenticated-orcid":false,"given":"Mahmoud A.","family":"Boualam","sequence":"additional","affiliation":[{"name":"MEPHI, Aix-Marseille University, IRD, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5276-6986","authenticated-orcid":false,"given":"Nadim","family":"Cassir","sequence":"additional","affiliation":[{"name":"MEPHI, Aix-Marseille University, IRD, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9mence","family":"Mimari","sequence":"additional","affiliation":[{"name":"Service ORL H\u00f4pital de la Conception, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delphine","family":"Poitrenaud","sequence":"additional","affiliation":[{"name":"Service Maladies Infectieuses, CH Ajaccio, 20000 Ajaccio, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2285-7051","authenticated-orcid":false,"given":"Soraya","family":"Mezouar","sequence":"additional","affiliation":[{"name":"MEPHI, Aix-Marseille University, IRD, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean Louis","family":"M\u00e8ge","sequence":"additional","affiliation":[{"name":"MEPHI, Aix-Marseille University, IRD, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Drancourt","sequence":"additional","affiliation":[{"name":"MEPHI, Aix-Marseille University, IRD, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hubert","family":"Lepidi","sequence":"additional","affiliation":[{"name":"MEPHI, Aix-Marseille University, IRD, 13005 Marseille, France"},{"name":"IHU M\u00e9diterran\u00e9e Infection, 13005 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2023,11,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"S26","DOI":"10.1016\/j.ijid.2022.03.011","article-title":"The WHO Global Tuberculosis 2021 Report\u2014Not so Good News and Turning the Tide Back to End TB","volume":"124","author":"Chakaya","year":"2022","journal-title":"Int. J. Infect. Dis."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1007\/s12105-018-0970-y","article-title":"Primary Laryngeal Tuberculosis: A Series of 15 Cases","volume":"13","author":"Agarwal","year":"2019","journal-title":"Head Neck Pathol."},{"key":"ref_3","first-page":"175","article-title":"Laryngeal Tuberculosis: A Case Report","volume":"9","author":"Ulozas","year":"2021","journal-title":"J. Med. Sci."},{"key":"ref_4","first-page":"140","article-title":"Laryngeal Case for Diagnosis","volume":"2","year":"1909","journal-title":"Proc. R. Soc. Med."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1186\/s13256-015-0548-1","article-title":"Laryngeal Tuberculosis Diagnosed by Stool Sample Cultures: A Case Report","volume":"9","author":"Yin","year":"2015","journal-title":"J. Med. Case Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"e23770","DOI":"10.1097\/MD.0000000000023770","article-title":"Appearance and Morphologic Features of Laryngeal Tuberculosis Using Laryngoscopy","volume":"99","author":"Zang","year":"2020","journal-title":"Medicine"},{"key":"ref_7","first-page":"97","article-title":"Laryngeal and Voice Disorders in Patients with Pulmonary Tuberculosis","volume":"33","author":"Youssef","year":"2021","journal-title":"Iran. J. Otorhinolaryngol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"582","DOI":"10.1016\/j.otohns.2007.04.002","article-title":"Laryngeal Tuberculosis: A Review of 26 Cases","volume":"137","author":"Wang","year":"2007","journal-title":"Otolaryngol.\u2014Head Neck Surg."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1007\/s00405-006-0063-5","article-title":"Current Clinical Propensity of Laryngeal Tuberculosis: Review of 60 Cases","volume":"263","author":"Lim","year":"2006","journal-title":"Eur. Arch. Oto-Rhino-Laryngol. Head Neck"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Benhoummad, O., Rochd, S., Yafi, M., Rochdi, Y., and Raji, A. (2022). Extraganglionary Localization of Tuberculosis in Otorhinolaryngology: Rare Presentations About 29 Cases. Med. Res. Arch., 10.","DOI":"10.18103\/mra.v10i9.3062"},{"key":"ref_11","first-page":"347","article-title":"La tuberculose primitive du larynx","volume":"131","author":"Chariba","year":"2014","journal-title":"Ann. Fr. Oto-Rhino-Laryngol. Pathol. Cervico-Faciale"},{"key":"ref_12","first-page":"411","article-title":"Laryngeal Tuberculosis in Renal Transplant Recipients: A Case Report and Review of the Literature","volume":"20","author":"Cialente","year":"2020","journal-title":"Bosn. J. Basic Med. Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1097\/INF.0000000000002565","article-title":"Shell-Vial Assay in Diagnosis of Disseminated BCG Infection in an Immunodeficient Child","volume":"39","author":"Fellag","year":"2020","journal-title":"Pediatr. Infect. Dis. J."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Robinne, S., Saad, J., Morsli, M., Hamidou, Z.H., Tazerart, F., Drancourt, M., and Baron, S.A. (2022). Rapid Identification of Mycobacterium tuberculosis Complex Using Mass Spectrometry: A Proof of Concept. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.753969"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Loukil, A., Kirtania, P., Bedotto, M., and Drancourt, M. (2018). FISHing Mycobacterium tuberculosis Complex by Use of a rpoB DNA Probe Bait. J. Clin. Microbiol., 56.","DOI":"10.1128\/JCM.00568-18"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"47","DOI":"10.4274\/tao.2021.2021-7-1","article-title":"Primary Laryngeal Tuberculosis Manifesting as Irregular Vocal Fold Lesion","volume":"60","author":"Guan","year":"2022","journal-title":"Turk. Arch. Otorhinolaryngol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"109151","DOI":"10.1016\/j.dib.2023.109151","article-title":"Draft Genome Sequence Data of Enterococcus Faecium R9, a Multiple Enterocins-Producing Strain","volume":"48","author":"Akbar","year":"2023","journal-title":"Data Brief"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"250","DOI":"10.1016\/S1473-3099(13)70253-6","article-title":"Is Tuberculosis a Lymphatic Disease with a Pulmonary Portal?","volume":"14","author":"Behr","year":"2014","journal-title":"Lancet Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"407","DOI":"10.14639\/0392-100X-N2091","article-title":"Laryngeal Tubercolosis: A Case Report with Focus on Voice Assessment and Review of the Literature","volume":"42","author":"Migliorelli","year":"2022","journal-title":"ACTA Otorhinolaryngol. Ital."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1016\/j.anl.2015.02.012","article-title":"Laryngeal Tuberculosis: A Report of 17 Cases","volume":"42","author":"Kurokawa","year":"2015","journal-title":"Auris. Nasus. Larynx"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/S0196-0709(00)85010-3","article-title":"Laryngeal Tuberculosis","volume":"21","author":"Yencha","year":"2000","journal-title":"Am. J. Otolaryngol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e230","DOI":"10.1016\/j.ijid.2009.05.002","article-title":"Changing Trends in the Clinical Features of Laryngeal Tuberculosis: A Report of 19 Cases","volume":"14","author":"Ling","year":"2010","journal-title":"Int. J. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"555","DOI":"10.1258\/0022215011908487","article-title":"Epidemiological considerations and clinical features of ENT tuberculosis","volume":"115","author":"Kulkarni","year":"2001","journal-title":"J. Laryngol. Otol."},{"key":"ref_24","first-page":"638","article-title":"Clinical Features of Laryngeal Tuberculosis in Iran","volume":"51","author":"Hasibi","year":"2013","journal-title":"Acta Med. Iran."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1177\/0003489419875755","article-title":"Atypical Laryngeal Infections: Localized Lesions from Unusual Organisms May Simulate Malignancy","volume":"129","author":"Yan","year":"2020","journal-title":"Ann. Otol. Rhinol. Laryngol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5","DOI":"10.3166\/bspe-2018-0010","article-title":"La tuberculose laryng\u00e9e diagnostiqu\u00e9e dans un laboratoire d\u2019anatomie pathologique du S\u00e9n\u00e9gal (2011\u20132015)","volume":"111","author":"Thiam","year":"2018","journal-title":"Bull. Soc. Pathol. 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