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This infection poses significant risks, leading to severe complications in immunocompromised individuals and potentially affecting the fetus through congenital transmission. A comprehensive understanding of the intricate molecular interactions between T. gondii and its host is pivotal for the development of effective therapeutic strategies. This review emphasizes the crucial role of proteomics in T. gondii research, with a specific focus on host\u2013parasite interactions, post-translational modifications (PTMs), PTM crosstalk, and ongoing efforts in drug discovery. Additionally, we provide an overview of recent advancements in proteomics techniques, encompassing interactome sample preparation methods such as BioID (BirA*-mediated proximity-dependent biotin identification), APEX (ascorbate peroxidase-mediated proximity labeling), and Y2H (yeast two hybrid), as well as various proteomics approaches, including single-cell analysis, DIA (data-independent acquisition), targeted, top-down, and plasma proteomics. Furthermore, we discuss bioinformatics and the integration of proteomics with other omics technologies, highlighting its potential in unraveling the intricate mechanisms of T. gondii pathogenesis and identifying novel therapeutic targets.<\/jats:p>","DOI":"10.3390\/pathogens13010033","type":"journal-article","created":{"date-parts":[[2023,12,29]],"date-time":"2023-12-29T06:18:13Z","timestamp":1703830693000},"page":"33","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Proteomics Applications in Toxoplasma gondii: Unveiling the Host\u2013Parasite Interactions and Therapeutic Target Discovery"],"prefix":"10.3390","volume":"13","author":[{"given":"Bin","family":"Deng","sequence":"first","affiliation":[{"name":"Department of Biology and VBRN Proteomics Facility, University of Vermont, Burlington, VT 05405, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Vanagas","sequence":"additional","affiliation":[{"name":"Laboratorio de Parasitolog\u00eda Molecular, Instituto Tecnol\u00f3gico de Chascom\u00fas (CONICET-UNSAM), Chascom\u00fas 7130, Provincia de Buenos Aires, Argentina"},{"name":"Escuela de Bio y Nanotecnolog\u00edas (UNSAM), 25 de Mayo y Francia. 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