{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T01:14:24Z","timestamp":1788484464480,"version":"build-2803163510"},"reference-count":55,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T00:00:00Z","timestamp":1704758400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Swiss National Science Foundation\u2014Bridge Discovery","award":["40B20_203615"],"award-info":[{"award-number":["40B20_203615"]}]},{"name":"Multidisciplinary Center for Infectious Diseases, Bern, Switzerland","award":["40B20_203615"],"award-info":[{"award-number":["40B20_203615"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Whole-genome sequencing (WGS) represents the main technology for SARS-CoV-2 lineage characterization in diagnostic laboratories worldwide. The rapid, near-full-length sequencing of the viral genome is commonly enabled by high-throughput sequencing of PCR amplicons derived from cDNA molecules. Here, we present a new approach called NASCarD (Nanopore Adaptive Sampling with Carrier DNA), which allows a low amount of nucleic acids to be sequenced while selectively enriching for sequences of interest, hence limiting the production of non-target sequences. Using COVID-19 positive samples available during the omicron wave, we demonstrate how the method may lead to &gt;99% genome completeness of the SARS-CoV-2 genome sequences within 7 h of sequencing at a competitive cost. The new approach may have applications beyond SARS-CoV-2 sequencing for other DNA or RNA pathogens in clinical samples.<\/jats:p>","DOI":"10.3390\/pathogens13010061","type":"journal-article","created":{"date-parts":[[2024,1,9]],"date-time":"2024-01-09T04:38:51Z","timestamp":1704775131000},"page":"61","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["NASCarD (Nanopore Adaptive Sampling with Carrier DNA): A Rapid, PCR-Free Method for SARS-CoV-2 Whole-Genome Sequencing in Clinical Samples"],"prefix":"10.3390","volume":"13","author":[{"given":"Miguel A.","family":"Terrazos Miani","sequence":"first","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Friedb\u00fchlstrasse 25, 3001 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lo\u00efc","family":"Borcard","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Friedb\u00fchlstrasse 25, 3001 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sonja","family":"Gempeler","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Friedb\u00fchlstrasse 25, 3001 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Baumann","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Friedb\u00fchlstrasse 25, 3001 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pascal","family":"Bittel","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Friedb\u00fchlstrasse 25, 3001 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen L.","family":"Leib","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Friedb\u00fchlstrasse 25, 3001 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Neuenschwander","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Friedb\u00fchlstrasse 25, 3001 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3437-4639","authenticated-orcid":false,"given":"Alban","family":"Ramette","sequence":"additional","affiliation":[{"name":"Institute for Infectious Diseases, University of Bern, Friedb\u00fchlstrasse 25, 3001 Bern, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,9]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1056\/NEJMoa2001017","article-title":"A novel coronavirus from patients with pneumonia in China, 2019","volume":"382","author":"Zhu","year":"2020","journal-title":"N. Engl. J. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e055781","DOI":"10.1136\/bmjopen-2021-055781","article-title":"Public health implications of SARS-CoV-2 variants of concern: A rapid scoping review","volume":"11","author":"Somerville","year":"2021","journal-title":"BMJ Open"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Vogels, C.B.F., Breban, M.I., Ott, I.M., Alpert, T., Petrone, M.E., Watkins, A.E., Kalinich, C.C., Earnest, R., Rothman, J.E., and Goes de Jesus, J. (2021). Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2. PLoS Biol., 19.","DOI":"10.1371\/journal.pbio.3001236"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"104814","DOI":"10.1016\/j.jcv.2021.104814","article-title":"Implementation of an in-house real-time reverse transcription-PCR assay for the rapid detection of the SARS-CoV-2 Marseille-4 variant","volume":"139","author":"Bedotto","year":"2021","journal-title":"J. Clin. Virol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"102892","DOI":"10.1016\/j.isci.2021.102892","article-title":"A benchmarking study of SARS-CoV-2 whole-genome sequencing protocols using COVID-19 patient samples","volume":"24","author":"Liu","year":"2021","journal-title":"iScience"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Freed, N.E., Vlkov\u00e1, M., Faisal, M.B., and Silander, O.K. (2020). Rapid and inexpensive whole-genome sequencing of SARS-CoV-2 using 1200 bp tiled amplicons and Oxford Nanopore Rapid Barcoding. Biol. Methods Protoc., 5.","DOI":"10.1093\/biomethods\/bpaa014"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1261","DOI":"10.1038\/nprot.2017.066","article-title":"Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples","volume":"12","author":"Quick","year":"2017","journal-title":"Nat. Protoc."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Plitnick, J., Griesemer, S., Lasek-Nesselquist, E., Singh, N., Lamson, D.M., and St George, K. (2021). Whole-Genome Sequencing of SARS-CoV-2: Assessment of the Ion Torrent AmpliSeq Panel and Comparison with the Illumina MiSeq ARTIC Protocol. J. Clin. Microbiol., 59.","DOI":"10.1128\/JCM.00649-21"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4121","DOI":"10.1093\/bioinformatics\/bty407","article-title":"Nextstrain: Real-time tracking of pathogen evolution","volume":"34","author":"Hadfield","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1038\/s41564-020-0770-5","article-title":"A dynamic nomenclature proposal for SARS-CoV-2 lineages to assist genomic epidemiology","volume":"5","author":"Rambaut","year":"2020","journal-title":"Nat. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"veaa075","DOI":"10.1093\/ve\/veaa075","article-title":"Evaluation of NGS-based approaches for SARS-CoV-2 whole genome characterisation","volume":"6","author":"Charre","year":"2020","journal-title":"Virus Evol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Vrancken, B., Trov\u00e3o, N., Baele, G., Van Wijngaerden, E., Vandamme, A.M., Van Laethem, K., and Lemey, P. (2016). Quantifying Next Generation Sequencing Sample Pre-Processing Bias in HIV-1 Complete Genome Sequencing. Viruses, 8.","DOI":"10.3390\/v8010012"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.ijid.2021.10.050","article-title":"Mutations in emerging variant of concern lineages disrupt genomic sequencing of SARS-CoV-2 clinical specimens","volume":"114","author":"Kuchinski","year":"2022","journal-title":"Int. J. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"840952","DOI":"10.3389\/fviro.2022.840952","article-title":"Investigating the Extent of Primer Dropout in SARS-CoV-2 Genome Sequences during the Early Circulation of Delta Variants","volume":"2","author":"Borcard","year":"2022","journal-title":"Front. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Davis, J.J., Long, S.W., Christensen, P.A., Olsen, R.J., Olson, R., Shukla, M., Subedi, S., Stevens, R., and Musser, J.M. (2021). Analysis of the ARTIC Version 3 and Version 4 SARS-CoV-2 Primers and Their Impact on the Detection of the G142D Amino Acid Substitution in the Spike Protein. Microbiol. Spectr., 9.","DOI":"10.1128\/Spectrum.01803-21"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"836728","DOI":"10.3389\/fmed.2022.836728","article-title":"Optimization of the SARS-CoV-2 ARTIC Network V4 Primers and Whole Genome Sequencing Protocol","volume":"9","author":"Lambisia","year":"2022","journal-title":"Front. Med."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Chrzastek, K., Tennakoon, C., Bialy, D., Freimanis, G., Flannery, J., and Shelton, H. (2022). A random priming amplification method for whole genome sequencing of SARS-CoV-2 virus. BMC Genom., 23.","DOI":"10.1186\/s12864-022-08563-z"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1016\/j.virol.2017.06.019","article-title":"Use of Sequence-Independent, Single-Primer-Amplification (SISPA) for rapid detection, identification, and characterization of avian RNA viruses","volume":"509","author":"Chrzastek","year":"2017","journal-title":"Virology"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Marotz, C.A., Sanders, J.G., Zuniga, C., Zaramela, L.S., Knight, R., and Zengler, K. (2018). Improving saliva shotgun metagenomics by chemical host DNA depletion. Microbiome, 6.","DOI":"10.1186\/s40168-018-0426-3"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1101\/gr.247064.118","article-title":"Direct RNA nanopore sequencing of full-length coronavirus genomes provides novel insights into structural variants and enables modification analysis","volume":"29","author":"Viehweger","year":"2019","journal-title":"Genome Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Gr\u00e4del, C., Terrazos Miani, M.A., Baumann, C., Barbani, M.T., Neuenschwander, S., Leib, S.L., Suter-Riniker, F., and Ramette, A. (2020). Whole-Genome Sequencing of Human Enteroviruses from Clinical Samples by Nanopore Direct RNA Sequencing. Viruses, 12.","DOI":"10.1101\/2020.06.09.20126219"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Vacca, D., Fiannaca, A., Tramuto, F., Cancila, V., La Paglia, L., Mazzucco, W., Gulino, A., Rosa, M.L., Maida, C.M., and Morello, G. (2022). Direct RNA Nanopore Sequencing of SARS-CoV-2 Extracted from Critical Material from Swabs. Life, 12.","DOI":"10.3390\/life12010069"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1348","DOI":"10.1038\/s41587-021-01108-x","article-title":"Nanopore sequencing technology, bioinformatics and applications","volume":"39","author":"Wang","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e01315-19","DOI":"10.1128\/JCM.01315-19","article-title":"Third-Generation Sequencing in the Clinical Laboratory: Exploring the Advantages and Challenges of Nanopore Sequencing","volume":"58","author":"Petersen","year":"2019","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1128\/JCM.00060-20","article-title":"A Sample-to-Report Solution for Taxonomic Identification of Cultured Bacteria in the Clinical Setting Based on Nanopore Sequencing","volume":"58","author":"Neuenschwander","year":"2020","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1038\/nmeth.3930","article-title":"Real-time selective sequencing using nanopore technology","volume":"13","author":"Loose","year":"2016","journal-title":"Nat. Methods"},{"key":"ref_27","unstructured":"Oxford_Nanopore_Technologies (2023, March 03). Read Until-API. Available online: https:\/\/github.com\/nanoporetech\/read_until_api."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"431","DOI":"10.1038\/s41587-020-0731-9","article-title":"Targeted nanopore sequencing by real-time mapping of raw electrical signal with UNCALLED","volume":"39","author":"Kovaka","year":"2020","journal-title":"Nat. Biotechnol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1038\/s41587-020-00746-x","article-title":"Readfish enables targeted nanopore sequencing of gigabase-sized genomes","volume":"39","author":"Payne","year":"2021","journal-title":"Nat. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kipp, E.J., Lindsey, L.L., Khoo, B.S., Faulk, C.D., Oliver, J.D., and Larsen, P.A. (2021). Enabling metagenomic surveillance for bacterial tick-borne pathogens using nanopore sequencing with adaptive sampling. bioRxiv.","DOI":"10.1101\/2021.08.17.456696"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Lin, Y., Dai, Y., Liu, Y., Ren, Z., Guo, H., Li, Z., Li, J., Wang, K., Yang, L., and Zhang, S. (2022). Rapid PCR-Based Nanopore Adaptive Sequencing Improves Sensitivity and Timeliness of Viral Clinical Detection and Genome Surveillance. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.929241"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Martin, S., Heavens, D., Lan, Y., Horsfield, S., Clark, M.D., and Leggett, R.M. (2022). Nanopore adaptive sampling: A tool for enrichment of low abundance species in metagenomic samples. Genome Biol., 23.","DOI":"10.1186\/s13059-021-02582-x"},{"key":"ref_33","unstructured":"Heavens, D., Chooneea, D., Giolai, M., Cuber, P., Aanstad, P., Martin, S., Alston, M., Misra, R., Clark, M.D., and Leggett, R.M. (2023, February 18). How Low Can You Go? Driving down the DNA Input Requirements for Nanopore Sequencing. Available online: https:\/\/www.biorxiv.org\/content\/10.1101\/2021.10.15.464554v1."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"30494","DOI":"10.2807\/1560-7917.ES.2017.22.13.30494","article-title":"GISAID: Global initiative on sharing all influenza data\u2014From vision to reality","volume":"22","author":"Shu","year":"2017","journal-title":"Eurosurveillance"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"giab008","DOI":"10.1093\/gigascience\/giab008","article-title":"Twelve years of SAMtools and BCFtools","volume":"10","author":"Danecek","year":"2021","journal-title":"GigaScience"},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Shen, W., Le, S., Li, Y., and Hu, F. (2016). SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA\/Q File Manipulation. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0163962"},{"key":"ref_37","unstructured":"Loman, N., Rowe, W., and Rambaut, A. (2023, March 03). nCoV-2019 Novel Coronavirus Bioinformatics Protocol. Available online: https:\/\/artic.network\/ncov-2019\/ncov2019-bioinformatics-sop.html."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"568","DOI":"10.1093\/bioinformatics\/btaa716","article-title":"Mutation-Simulator: Fine-grained simulation of random mutations in any genome","volume":"37","author":"Stich","year":"2021","journal-title":"Bioinformatics"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Quick, J. (2023, March 03). nCoV-2019 Sequencing Protocol v3 (LoCost). Available online: https:\/\/protocols.io\/view\/ncov-2019-sequencing-protocol-v3-locost-bh42j8ye.","DOI":"10.17504\/protocols.io.bp2l6n26rgqe\/v3"},{"key":"ref_40","unstructured":"(2023, March 03). Artic-Network\/Fieldbioinformatics. Available online: https:\/\/github.com\/artic-network\/fieldbioinformatics\/tree\/master\/artic."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"3094","DOI":"10.1093\/bioinformatics\/bty191","article-title":"Minimap2: Pairwise alignment for nucleotide sequences","volume":"34","author":"Li","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Mojarro, A., Hachey, J., Ruvkun, G., Zuber, M.T., and Carr, C.E. (2018). CarrierSeq: A sequence analysis workflow for low-input nanopore sequencing. BMC Bioinform., 19.","DOI":"10.1186\/s12859-018-2124-3"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.coviro.2011.12.004","article-title":"Metagenomics and future perspectives in virus discovery","volume":"2","author":"Mokili","year":"2012","journal-title":"Curr. Opin. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1080\/22221751.2021.1872351","article-title":"Characterization of SARS-CoV-2 ORF6 deletion variants detected in a nosocomial cluster during routine genomic surveillance, Lyon, France","volume":"10","author":"Destras","year":"2021","journal-title":"Emerg. Microbes Infect."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"960","DOI":"10.1016\/j.cmi.2020.03.020","article-title":"Molecular characterization of SARS-CoV-2 in the first COVID-19 cluster in France reveals an amino acid deletion in nsp2 (Asp268del)","volume":"26","author":"Bal","year":"2020","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2002169","DOI":"10.1002\/smll.202002169","article-title":"Nanopore Targeted Sequencing for the Accurate and Comprehensive Detection of SARS-CoV-2 and Other Respiratory Viruses","volume":"16","author":"Wang","year":"2020","journal-title":"Small"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"4000","DOI":"10.1038\/s41598-022-08003-8","article-title":"Evaluation of microbiome enrichment and host DNA depletion in human vaginal samples using Oxford Nanopore\u2019s adaptive sequencing","volume":"12","author":"Marquet","year":"2022","journal-title":"Sci. Rep."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Gan, M., Wu, B., Yan, G., Li, G., Sun, L., Lu, G., and Zhou, W. (2021). Combined nanopore adaptive sequencing and enzyme-based host depletion efficiently enriched microbial sequences and identified missing respiratory pathogens. BMC Genom., 22.","DOI":"10.1186\/s12864-021-08023-0"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Bloomfield, S.J., Zomer, A.L., O\u2019Grady, J., Kay, G.L., Wain, J., Janecko, N., Palau, R., and Mather, A.E. (2023). Determination and quantification of microbial communities and antimicrobial resistance on food through host DNA-depleted metagenomics. Food Microbiol., 110.","DOI":"10.1016\/j.fm.2022.104162"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1059","DOI":"10.1007\/s10096-020-03913-9","article-title":"Viral RNA load as determined by cell culture as a management tool for discharge of SARS-CoV-2 patients from infectious disease wards","volume":"39","author":"Andreani","year":"2020","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1038\/s41586-020-2196-x","article-title":"Virological assessment of hospitalized patients with COVID-2019","volume":"581","author":"Corman","year":"2020","journal-title":"Nature"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/j.cell.2020.04.021","article-title":"Coast-to-Coast Spread of SARS-CoV-2 during the Early Epidemic in the United States","volume":"181","author":"Fauver","year":"2020","journal-title":"Cell"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Sahajpal, N.S., Mondal, A.K., Njau, A., Petty, Z., Chen, J., Ananth, S., Ahluwalia, P., Williams, C., Ross, T.M., and Chaubey, A. (2021). High-Throughput Next-Generation Sequencing Respiratory Viral Panel: A Diagnostic and Epidemiologic Tool for SARS-CoV-2 and Other Viruses. Viruses, 13.","DOI":"10.3390\/v13102063"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"i153","DOI":"10.1093\/bioinformatics\/btac223","article-title":"ReadBouncer: Precise and scalable adaptive sampling for nanopore sequencing","volume":"38","author":"Ulrich","year":"2022","journal-title":"Bioinformatics"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"e00197-21","DOI":"10.1128\/Spectrum.00197-21","article-title":"Targeted Hybridization Capture of SARS-CoV-2 and Metagenomics Enables Genetic Variant Discovery and Nasal Microbiome Insights","volume":"9","author":"Wells","year":"2021","journal-title":"Microbiol. Spectr."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/1\/61\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:42:32Z","timestamp":1760103752000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/1\/61"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,9]]},"references-count":55,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,1]]}},"alternative-id":["pathogens13010061"],"URL":"https:\/\/doi.org\/10.3390\/pathogens13010061","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,9]]}}}