{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:42:06Z","timestamp":1793313726940,"version":"4.5.0"},"reference-count":31,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T00:00:00Z","timestamp":1706227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fapesp","award":["2019\/20573-1"],"award-info":[{"award-number":["2019\/20573-1"]}]},{"name":"Fapesp","award":["2019\/18581-6"],"award-info":[{"award-number":["2019\/18581-6"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>In this new methodology, plasmonic ELISA (pELISA) was used to detect Circovirus porcine2 (PCV2) in serum samples without the need for plate reading equipment. This process occurs by adapting the conventional ELISA test with gold nanoparticles (AuNPs) to promote a color change on the plate and quickly identify this difference with the naked eye, generating a dark purple-gray hue when the samples are positive and red when the samples are negative. The technique demonstrated high efficiency in detecting samples with a viral load \u2265 5 log10 copies\/mL. Plasmonic ELISA offers user-friendly, cost-effective, and reliable characteristics, making it a valuable tool for PCV2 diagnosis and potentially adaptable for other pathogen detection applications.<\/jats:p>","DOI":"10.3390\/pathogens13020108","type":"journal-article","created":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T04:05:46Z","timestamp":1706241946000},"page":"108","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Development of a Gold Nanoparticle-Based ELISA for Detection of PCV2"],"prefix":"10.3390","volume":"13","author":[{"given":"Caroline Rodrigues","family":"Basso","sequence":"first","affiliation":[{"name":"Biotechnology Institute, S\u00e3o Paulo State University, Botucatu 18607-440, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8570-1038","authenticated-orcid":false,"given":"Ta\u00eds Fukuta","family":"Cruz","sequence":"additional","affiliation":[{"name":"Biotechnology Institute, S\u00e3o Paulo State University, Botucatu 18607-440, SP, Brazil"},{"name":"Chemical and Biological Sciences Department, Bioscience Institute, S\u00e3o Paulo State University, Botucatu 18618-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4458-8315","authenticated-orcid":false,"given":"Larissa Baldo","family":"Vieira","sequence":"additional","affiliation":[{"name":"Biotechnology Institute, S\u00e3o Paulo State University, Botucatu 18607-440, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9950-7711","authenticated-orcid":false,"given":"Valber de Albuquerque","family":"Pedrosa","sequence":"additional","affiliation":[{"name":"Chemical and Biological Sciences Department, Bioscience Institute, S\u00e3o Paulo State University, Botucatu 18618-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0118-6164","authenticated-orcid":false,"given":"F\u00e1bio Sossai","family":"Possebon","sequence":"additional","affiliation":[{"name":"Biotechnology Institute, S\u00e3o Paulo State University, Botucatu 18607-440, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9153-1485","authenticated-orcid":false,"given":"Jo\u00e3o Pessoa","family":"Araujo Junior","sequence":"additional","affiliation":[{"name":"Biotechnology Institute, S\u00e3o Paulo State University, Botucatu 18607-440, SP, Brazil"},{"name":"Chemical and Biological Sciences Department, Bioscience Institute, S\u00e3o Paulo State University, Botucatu 18618-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Guo, J., Hou, L., Zhou, J., Wang, D., Cui, Y., Feng, X., and Liu, J. 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