{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T22:12:12Z","timestamp":1780438332547,"version":"3.54.1"},"reference-count":44,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T00:00:00Z","timestamp":1706227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Loewe Center Druid","award":["C4"],"award-info":[{"award-number":["C4"]}]},{"name":"Loewe Center Druid","award":["310313\/2019\u20138"],"award-info":[{"award-number":["310313\/2019\u20138"]}]},{"name":"Loewe Center Druid","award":["RED-00313\u201316"],"award-info":[{"award-number":["RED-00313\u201316"]}]},{"name":"Conselho Nacional de Pesquisa e Desenvolvimento Tecnol\u00f3gico","award":["C4"],"award-info":[{"award-number":["C4"]}]},{"name":"Conselho Nacional de Pesquisa e Desenvolvimento Tecnol\u00f3gico","award":["310313\/2019\u20138"],"award-info":[{"award-number":["310313\/2019\u20138"]}]},{"name":"Conselho Nacional de Pesquisa e Desenvolvimento Tecnol\u00f3gico","award":["RED-00313\u201316"],"award-info":[{"award-number":["RED-00313\u201316"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de Minas Gerais","award":["C4"],"award-info":[{"award-number":["C4"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de Minas Gerais","award":["310313\/2019\u20138"],"award-info":[{"award-number":["310313\/2019\u20138"]}]},{"name":"Funda\u00e7\u00e3o de Amparo a Pesquisa do Estado de Minas Gerais","award":["RED-00313\u201316"],"award-info":[{"award-number":["RED-00313\u201316"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Control of canine infections with Leishmania infantum (L. infantum), a major zoonotic disease in Brazil and southern Europe, is becoming increasingly important due to its close proximity to humans, the increasing import of dogs from endemic regions and the impact of climate change on vector spreading. Simple, rapid and reliable diagnostic tests are therefore needed to detect infected dogs. Here, we re-evaluated different serological methods for the diagnosis of canine leishmaniosis (CanL) in Croatia and Brazil. The diagnostic performance of the indirect fluorescent antibody test (IFAT) and the VetLine\u00ae Leishmania ELISA (GSD Frankfurt, Germany) was compared with three rKLi8.3-based diagnostic test systems, the rKLi8.3 ELISA (GSD Frankfurt, Germany), the INgezim\u00ae Leishma CROM (GSD Madrid, Spain) lateral flow test (LFT) and the VetBlot\u00ae\u00a0Leishmania LineBlot (GSD Frankfurt, Germany). CanL symptomatic dogs were efficiently diagnosed by all tests, except the VetLine\u00ae Leishmania ELISA, which is based on whole Leishmania antigens. The advantage of rKLi8.3 was also observed in oligo- and asymptomatic dogs from Brazil and Croatia, although with reduced diagnostic efficiency compared to symptomatic dogs. Similar to IFAT and rKLi8.3 ELISA, the LFT did not cross-react with other common canine pathogens; it showed very high specificity for healthy dogs from endemic regions in both countries and did not react with healthy, vaccinated dogs in Brazil. In conclusion, serodiagnostic tests based on the rKLi8.3 antigens are superior to whole parasite antigens, and the LFT has the advantage of providing a laboratory-independent, rapid and specific diagnosis of CanL.<\/jats:p>","DOI":"10.3390\/pathogens13020109","type":"journal-article","created":{"date-parts":[[2024,1,26]],"date-time":"2024-01-26T04:05:46Z","timestamp":1706241946000},"page":"109","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Comparative Study of a Novel Lateral Flow Rapid Test with Conventional Serological Test Systems for the Diagnosis of Canine Leishmaniosis in Croatia and Brazil"],"prefix":"10.3390","volume":"13","author":[{"given":"Rouzbeh","family":"Mahdavi","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology and Hospital Hygiene, Phillips University of Marburg, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franjo","family":"Martinkovic","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Zagreb, HR-1000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hosam","family":"Shams-Eldin","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hospital Hygiene, Phillips University of Marburg, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid E.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8123-4164","authenticated-orcid":false,"given":"Alexandre B.","family":"Reis","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Federal University of Ouro Preto, Ouro Preto 35400-000, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Latz","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Frankfurt (GSD Frankfurt), 63128 Dietzenbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Heinz","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Frankfurt (GSD Frankfurt), 63128 Dietzenbach, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Aira","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Madrid S.A. (GSD Madrid), 28037 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7103-8522","authenticated-orcid":false,"given":"Alba","family":"Fresco-Taboada","sequence":"additional","affiliation":[{"name":"Gold Standard Diagnostics Madrid S.A. (GSD Madrid), 28037 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0156-7346","authenticated-orcid":false,"given":"Elfadil","family":"Abass","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Science, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, Dammam 34212, Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jelena","family":"Romero-Olmedo","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hospital Hygiene, Phillips University of Marburg, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0172-9720","authenticated-orcid":false,"given":"Henrique C.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Microbiology and Immunology, Federal University of Juiz de Fora, Juiz de Fora 36036-900, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2141-7756","authenticated-orcid":false,"given":"Ulrich","family":"Steinhoff","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology and Hospital Hygiene, Phillips University of Marburg, 35043 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1269","DOI":"10.1016\/S0020-7519(00)00136-3","article-title":"The leishmaniases as emerging and reemerging zoonoses","volume":"30","author":"Ashford","year":"2000","journal-title":"Int. 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