{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T13:30:14Z","timestamp":1791639014415,"version":"4.3.4"},"reference-count":156,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T00:00:00Z","timestamp":1706486400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>More than one million curable sexually transmitted infections occur every day. Trichomonas vaginalis is one of the main infections responsible for these epidemiological data; however, the diagnosis of this protozoan is still mainly based on microscopic and culture identification. The commercialization of immunological tests and the development of molecular techniques have improved the sensitivity of classical methods. Nevertheless, the fact that trichomoniasis is a neglected parasitic infection hinders the development of novel techniques and their implementation in routine diagnosis. This review article shows the different methods developed to identify T. vaginalis in population and the difficulties in diagnosing male and asymptomatic patients. The importance of including this parasite in routine gynecological screening, especially in pregnant women, and the importance of considering T. vaginalis as an indicator of high-risk sexual behavior are also discussed.<\/jats:p>","DOI":"10.3390\/pathogens13020126","type":"journal-article","created":{"date-parts":[[2024,1,29]],"date-time":"2024-01-29T04:57:07Z","timestamp":1706504227000},"page":"126","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8687-1100","authenticated-orcid":false,"given":"Alexandra","family":"Ib\u00e1\u00f1ez-Escribano","sequence":"first","affiliation":[{"name":"Research Group Antiparasitic Epidemiology, Diagnostics and Therapy, PARADET, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ram\u00f3n y Cajal s\/n, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Jos\u00e9","family":"Nogal-Ruiz","sequence":"additional","affiliation":[{"name":"Research Group Antiparasitic Epidemiology, Diagnostics and Therapy, PARADET, Complutense University of Madrid, 28040 Madrid, Spain"},{"name":"Department of Microbiology and Parasitology, Faculty of Pharmacy, Complutense University of Madrid, Plaza Ram\u00f3n y Cajal s\/n, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,1,29]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2023, June 19). Sexually Transmitted Infections (STIs). Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/sexually-transmitted-infections-(stis)."},{"key":"ref_2","unstructured":"World Health Organization (2023, September 27). Global Health Sector Strategies on, Respectively, HIV, Viral Hepatitis and Sexually Transmitted Infections for the Period 2022\u20132030. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789240053779."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1116","DOI":"10.1038\/s41564-022-01177-x","article-title":"Sexually transmitted infections and female reproductive health","volume":"7","author":"Muzny","year":"2022","journal-title":"Nat. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"548","DOI":"10.2471\/BLT.18.228486","article-title":"Chlamydia, gonorrhoea, trichomoniasis and syphilis: Global prevalence and incidence estimates, 2016","volume":"97","author":"Rowley","year":"2019","journal-title":"Bull. World Health Organ."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1086\/511144","article-title":"Trichomonas vaginalis infection in male sexual partners: Implications for diagnosis, treatment, and prevention","volume":"44","author":"Miller","year":"2007","journal-title":"Clin. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1016\/j.idc.2013.06.003","article-title":"Trichomoniasis: The \u201cNeglected\u201d Sexually Transmitted Disease","volume":"27","author":"Meites","year":"2013","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1097\/OLQ.0b013e31827c08c3","article-title":"A Trich-y question: Should Trichomonas vaginalis infection be reportable?","volume":"40","author":"Hoots","year":"2013","journal-title":"Sex. Transm. Dis."},{"key":"ref_8","unstructured":"Centers for Disease Control and Prevention (2023, July 20). Parasites-Parasitic Infections in the United States, Available online: https:\/\/www.cdc.gov\/parasites\/npi\/index.html."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/s15010-015-0860-0","article-title":"Trichomonas vaginalis: Pathogenicity and potential role in human reproductive failure","volume":"44","author":"Mielczarek","year":"2016","journal-title":"Infection"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1016\/j.mpmed.2014.04.004","article-title":"Trichomoniasis","volume":"43","author":"Lewis","year":"2014","journal-title":"Medicine"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1136\/sextrans-2012-050893","article-title":"The clinical spectrum of Trichomonas vaginalis infection and challenges to management","volume":"89","author":"Muzny","year":"2013","journal-title":"Sex. Transm. Infect."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"418","DOI":"10.1136\/sextrans-2013-051075","article-title":"Global epidemiology of Trichomonas vaginalis","volume":"89","author":"Poole","year":"2013","journal-title":"Sex. Transm. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1086\/509934","article-title":"Trichomonas vaginalis infection: The most prevalent nonviral sexually transmitted infection receives the least public health attention","volume":"44","year":"2007","journal-title":"Clin. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1136\/sextrans-2018-053713","article-title":"Trichomonas vaginalis and HIV infection acquisition: A systematic review and meta-analysis","volume":"95","author":"Masha","year":"2019","journal-title":"Sex. Transm. Infect."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1136\/sextrans-2012-051005","article-title":"Trichomoniasis and HIV interactions: A review","volume":"89","author":"Kissinger","year":"2013","journal-title":"Sex. Transm. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1097\/OLQ.0b013e3181b38a4b","article-title":"Trichomoniasis and other sexually transmitted infections: Results from the 2001\u20132004 national health and nutrition examination surveys","volume":"36","author":"Allsworth","year":"2009","journal-title":"Sex. Transm. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1007\/s00404-009-1291-x","article-title":"High prevalence of high-risk human papillomavirus infection among women with Trichomonas vaginalis infection on monolayer cytology","volume":"282","author":"Fayt","year":"2010","journal-title":"Arch. Gynecol. Obstet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1097\/00007435-199707000-00008","article-title":"Trichomonas vaginalis associated with low birth weight and preterm delivery. The vaginal infections and prematurity study group","volume":"24","author":"Cotch","year":"1997","journal-title":"Sex. Transm. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1080\/028418600431003","article-title":"Gynecological infections as risk determinants of subsequent cervical neoplasia","volume":"39","author":"Viikki","year":"2000","journal-title":"Acta Oncol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1002\/ijgo.14763","article-title":"Trichomonas vaginalis infection is associated with increased risk of cervical carcinogenesis: A systematic review and meta-analysis of 470 000 patients","volume":"163","author":"Hammar","year":"2023","journal-title":"Int. J. Gynecol. Obstet."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Fazlollahpour-Naghibi, A., Bagheri, K., Almukhtar, M., Taha, S.R., Zadeh, M.S., Moghadam, K.B., Tadi, M.J., Rouholamin, S., Razavi, M., and Sepidarkish, M. (2023). Trichomonas vaginalis infection and risk of cervical neoplasia: A systematic review and meta-analysis. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0288443"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2","DOI":"10.3347\/PHD.22160","article-title":"Inflammatory response to Trichomonas vaginalis in the pathogenesis of prostatitis and benign prostatic hyperplasia","volume":"61","author":"Hann","year":"2023","journal-title":"Parasit. Host. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1007\/s10552-017-0919-6","article-title":"Trichomonas vaginalis infection and risk of prostate cancer: Associations by disease aggressiveness and race\/ethnicity in the PLCO Trial","volume":"28","author":"Marous","year":"2017","journal-title":"Cancer Causes Control"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Nagata, M., Tome, A., White, K., Wilkens, L.R., Park, S.-Y., Marchand, L.L., Haiman, C., and Hernandez, B.Y. (2023). No association of Trichomonas vaginalis seropositivity with advanced prostate cancer risk in the multiethnic cohort: A nested case-control study. Cancers, 15.","DOI":"10.3390\/cancers15215194"},{"key":"ref_25","first-page":"147","article-title":"Flagyl in the treatment of trichomoniasis","volume":"36","author":"Rodin","year":"1960","journal-title":"Brit. J. Vener. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1859","DOI":"10.1016\/j.clinthera.2005.12.012","article-title":"Tinidazole: A nitroimidazole antiprotozoal agent","volume":"27","author":"Fung","year":"2005","journal-title":"Clin. Therapeut."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1258","DOI":"10.1177\/0956462418785451","article-title":"2018 European (IUSTI\/WHO) International Union against sexually transmitted infections (IUSTI) World Health Organization (WHO) guideline on the management of vaginal discharge","volume":"29","author":"Sherrard","year":"2018","journal-title":"Int. J. STD AIDS"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"S152","DOI":"10.1093\/cid\/ciac030","article-title":"Diagnosis and management of Trichomonas vaginalis: Summary of evidence reviewed for the 2021 Centers for Disease Control and Prevention Sexually Transmitted Infections treatment guidelines","volume":"74","author":"Kissinger","year":"2022","journal-title":"Clin. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.sxmr.2021.12.004","article-title":"Secnidazole for Trichomoniasis in women and men","volume":"10","author":"Muzny","year":"2022","journal-title":"Sex. Med. Rev."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Kissinger, P. (2015). Trichomonas vaginalis: A review of epidemiologic, clinical and treatment issues. BMC Infect. Dis., 15.","DOI":"10.1186\/s12879-015-1055-0"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1038\/sj.cr.7290169","article-title":"Drug resistance in the sexually transmitted protozoan Trichomonas vaginalis","volume":"13","author":"Dunne","year":"2003","journal-title":"Cell Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"370.e1","DOI":"10.1016\/j.ajog.2007.10.795","article-title":"Management of Trichomonas vaginalis in women with suspected metronidazole sensitivity","volume":"198","author":"Helms","year":"2008","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_33","first-page":"CD000218","article-title":"Interventions for treating trichomoniasis in women","volume":"2","author":"Forna","year":"2003","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"S31","DOI":"10.1136\/sextrans-2016-053063","article-title":"Rapid and point-of-care tests for the diagnosis of Trichomonas vaginalis in women and men","volume":"93","author":"Gaydos","year":"2017","journal-title":"Sex. Transm. Infect."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Honigberg, B.M. (1990). Trichomonads Parasitic in Humans, Springer.","DOI":"10.1007\/978-1-4612-3224-7"},{"key":"ref_36","unstructured":"Ryan, E.T., Hill, D.R., Solomon, T., Aronson, N., and Endy, T.P. (2020). Hunter\u2019s Tropical Medicine and Emerging Infectious Diseases, Elsevier Health Sciences. [10th ed.]."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1128\/CMR.11.2.300","article-title":"Clinical and microbiological aspects of Trichomonas vaginalis","volume":"11","author":"Petrin","year":"1998","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_38","first-page":"3161","article-title":"Trichomonas vaginalis","volume":"Volume 2","author":"Bennett","year":"2015","journal-title":"Mandell, Douglas, and Bennett\u2019s Principles and Practice of Infectious Diseases"},{"key":"ref_39","first-page":"385","article-title":"Animalcules observes dans les mati\u00e8res purulentes et le produit des s\u00e9cr\u00e9tions des organes g\u00e9nitaux de l\u2019homme et de la femme","volume":"3","year":"1836","journal-title":"C. R. Acad. Sci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"25","DOI":"10.4103\/0253-7184.112866","article-title":"Trichomoniasis: How do we diagnose in a resource poor setting?","volume":"34","author":"Sivaranjini","year":"2013","journal-title":"Indian J. Sex. Transm. Dis. AIDS"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1136\/sextrans-2013-051057","article-title":"Modern diagnosis of Trichomonas vaginalis infection","volume":"89","author":"Hobbs","year":"2013","journal-title":"Sex. Transm. Infect."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1136\/jcp.2009.070920","article-title":"Prevalence of Trichomonas vaginalis infection in women attending a major gynaecological hospital in Greece: A cross-sectional study","volume":"63","author":"Piperaki","year":"2010","journal-title":"J. Clin. Pathol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1086\/376558","article-title":"High rates of Trichomonas vaginalis among men attending a sexually transmitted diseases clinic: Implications for screening and urethritis management","volume":"188","author":"Schwebke","year":"2003","journal-title":"J. Infect. Dis."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1097\/00007435-199503000-00003","article-title":"Trichomoniasis in men: Old issues and new data","volume":"22","author":"Krieger","year":"1995","journal-title":"Sex. Transm. Dis."},{"key":"ref_45","first-page":"5","article-title":"Evaluation of different staining techniques in the diagnosis of Trichomonas vaginalis infection in females of reproductive age group","volume":"8","author":"Khatoon","year":"2014","journal-title":"J. Clin. Diagn. Res."},{"key":"ref_46","first-page":"42","article-title":"Simultaneous isolation of Trichomonas vaginalis and collection of vaginal exudates","volume":"45","author":"Robertson","year":"1969","journal-title":"Br. J. Vener. Dis."},{"key":"ref_47","first-page":"289","article-title":"Polyester sponge swabs to facilitate examination for genital infection in women","volume":"47","author":"Oates","year":"1971","journal-title":"Br. J. Vener. Dis."},{"key":"ref_48","unstructured":"Cercenado-Mansilla, E., and Cant\u00f3n-Moreno, R. (2018). Procedimientos en Microbiolog\u00eda Cl\u00ednica, Sociedad Espa\u00f1ola de Enfermedades Infecciosas y Microbiolog\u00eda Cl\u00ednica (SEIMC). [24th ed.]."},{"key":"ref_49","unstructured":"Alcamo, I.E. (2001). Fundamentals of Microbiology, Jones and Bartlett Publishers. [6th ed.]."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1071\/SH13015","article-title":"Performance of the OSOM Trichomonas Rapid Test for diagnosis of Trichomonas vaginalis infection among women in Mysore","volume":"10","author":"Madhivanan","year":"2013","journal-title":"Indian Sex. Health"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"3749","DOI":"10.1128\/JCM.37.11.3749-3750.1999","article-title":"Viability of Trichomonas vaginalis in transport medium","volume":"37","author":"Beverly","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1024","DOI":"10.1016\/0002-9378(63)90096-6","article-title":"Trichomonas vaginalis: A statistical evaluation of diagnostic methods","volume":"87","author":"Martin","year":"1963","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_53","first-page":"450","article-title":"Comparison of direct microscopy and culture in the diagnosis of trichomoniasis","volume":"50","author":"McCann","year":"1974","journal-title":"Br. J. Vener. Dis."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1314","DOI":"10.1016\/0002-9378(59)90598-8","article-title":"An evaluation of routine culture examination for Trichomonas vaginalis and Candida","volume":"78","author":"Clark","year":"1959","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"28","DOI":"10.1258\/095646203321043228","article-title":"Shelf life of Trichomonas vaginalis","volume":"14","author":"Kingston","year":"2003","journal-title":"Int. J. STD AIDS"},{"key":"ref_56","first-page":"114","article-title":"Transport problems in public health bacteriology","volume":"47","author":"Stuart","year":"1956","journal-title":"Can. J. Public Health"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1915","DOI":"10.1007\/s00436-020-06688-2","article-title":"Trichomoniasis in a tertiary hospital of Madrid, Spain (2013\u20132017): Prevalence and pregnancy rate, coinfections, metronidazole resistance, and endosymbiosis","volume":"119","author":"Bolumburu","year":"2020","journal-title":"Parasitol. Res."},{"key":"ref_58","first-page":"296","article-title":"A modified formula for the preparation of Stuart\u2019s transport medium","volume":"58","author":"Amies","year":"1967","journal-title":"Can. J. Public Health"},{"key":"ref_59","first-page":"328","article-title":"Trichomonas vaginalis. Survival in solid Stuart\u2019s medium","volume":"45","author":"Nielsen","year":"1969","journal-title":"Br. J. Vener. Dis."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1136\/jcp.10.4.327","article-title":"A culture medium for Trichomonas vaginalis Donn\u00e9 and species of Candida","volume":"10","author":"Feinberg","year":"1957","journal-title":"J. Clin. Pathol."},{"key":"ref_61","first-page":"192","article-title":"Frequency of N. gonorrhoeae, T. vaginalis and C. albicans in female venereological patients","volume":"51","author":"Eriksson","year":"1975","journal-title":"Br. J. Vener. Dis."},{"key":"ref_62","first-page":"172","article-title":"Studies in trichomoniasis in males","volume":"37","author":"Hoffman","year":"1961","journal-title":"Br. J. Vener. Dis."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1111\/j.1471-0528.1966.tb06091.x","article-title":"A clinical laboratory study of trichomoniasis of the female genital tract","volume":"73","author":"Hughes","year":"1966","journal-title":"Br. J. Obstet. Ginecol."},{"key":"ref_64","first-page":"1235","article-title":"A modified staining technique for Trichomonas vaginalis","volume":"32","author":"Freeman","year":"1958","journal-title":"S. Afr. Med. J."},{"key":"ref_65","unstructured":"Lowe, G.H. (1972). Laboratory Diagnosis of Venereal Disease, H. M. Stationery Office."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"966","DOI":"10.2307\/3279252","article-title":"A method for the diagnosis of Trichomonas vaginalis using acridine orange","volume":"61","author":"Fripp","year":"1975","journal-title":"J. Parasitol."},{"key":"ref_67","first-page":"85","article-title":"A comparison of five methods for the detection of Trichomonas vaginalis in clinical specimens","volume":"37","author":"Levett","year":"1980","journal-title":"Med. Lab. Sci."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"741","DOI":"10.1093\/ajcp\/59.5.741","article-title":"Adequate staining of Trichomonas vaginalis by McManus\u2019 periodic acid-Schiff stain","volume":"59","year":"1973","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/0002-9378(70)90496-5","article-title":"Clinical and laboratory studies on vaginal trichomoniasis","volume":"106","author":"Nagesha","year":"1970","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"120","DOI":"10.2307\/3271220","article-title":"Morphological Studies on Trichomonas","volume":"12","author":"Tanabe","year":"1926","journal-title":"J. Parasitol."},{"key":"ref_71","first-page":"602","article-title":"Trois techniques de recherche du Trichomonas vaginalis: Leurs valeurs compar\u00e9es","volume":"62","author":"Sorel","year":"1954","journal-title":"Presse Med."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1136\/jcp.18.4.432","article-title":"A comparison of current laboratory methods with a new semisolid culture medium for the detection of Trichomonas vaginalis","volume":"18","author":"Lowe","year":"1965","journal-title":"J. Clin. Pathol."},{"key":"ref_73","first-page":"56","article-title":"A study of the possible value of acridine orange-O stain in the diagnosis of Trichomonas vaginalis infection","volume":"23","author":"Rogers","year":"1977","journal-title":"Cent. Afr. J. Med."},{"key":"ref_74","first-page":"499","article-title":"Usefulness of staining specimens with acridine orange in the diagnosis of Trichomonas vaginalis","volume":"30","author":"Buharowski","year":"1984","journal-title":"Wiad. Parazytol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1136\/jcp.29.2.154","article-title":"Comparison of four techniques for the routine diagnosis of Trichomonas vaginalis infection","volume":"29","author":"Mason","year":"1976","journal-title":"J. Clin. Pathol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1093\/ajcp\/7.ts1.2.40","article-title":"Contrast stain for the rapid identification of Trichomonas vaginalis","volume":"7","author":"Miller","year":"1937","journal-title":"Am. J. Clin. Pathol."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1099\/00222615-1-1-153","article-title":"The laboratory diagnosis of vaginal infections caused by Trichomonas and Candida (Monilia) species","volume":"1","author":"Eddie","year":"1968","journal-title":"J. Med. Microbiol."},{"key":"ref_78","first-page":"475","article-title":"Valeur de la spermoculture dans ie diagnostic de la trichomonase urogenitale chez I\u2019homme","volume":"12","author":"Perju","year":"1966","journal-title":"Wiad. Parazytol."},{"key":"ref_79","first-page":"43","article-title":"Vital staining of Trichomonas vaginalis with fluorescein","volume":"30","author":"Coutts","year":"1954","journal-title":"Br. J. Vener. Dis."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1097\/00007435-198907000-00002","article-title":"Comparison of direct fluorescent antibody, acridine orange, wet mount, and culture for detection of Trichomonas vaginalis in women attending a public sexually transmitted diseases clinic","volume":"16","author":"Bickley","year":"1989","journal-title":"Sex Transm Dis."},{"key":"ref_81","first-page":"116","article-title":"Value of Papanicolaou-stained smears in the diagnosis of trichomoniasis, candidiasis and cervical herpes simplex virus infection in women","volume":"51","author":"Thin","year":"1975","journal-title":"Br. J. Vener. Dis."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1128\/jcm.25.7.1275-1279.1987","article-title":"Cell culture compared with broth for detection of Trichomonas vaginalis","volume":"25","author":"Garber","year":"1987","journal-title":"J. Clin. Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"1869","DOI":"10.1128\/jcm.26.9.1869-1870.1988","article-title":"Comparison of four methods to detect Trichomonas vaginalis","volume":"26","author":"Thomason","year":"1988","journal-title":"J. Clin. Microbiol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.1128\/jcm.27.6.1230-1233.1989","article-title":"Evaluation of six media for the growth of Trichomonas vaginalis from vaginal secretions","volume":"27","author":"Schmid","year":"1986","journal-title":"J. Clin. Microbiol."},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"22","DOI":"10.4103\/0974-777X.93756","article-title":"Diagnosis of Trichomonas vaginalis from vaginal specimens by wet mount microscopy, InPouch TV culture system, and PCR","volume":"4","author":"Patil","year":"2012","journal-title":"J. Glob. Infect. Dis."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/S0094-0143(21)00849-1","article-title":"Sexually transmitted protozoal infections. Trichomonas vaginalis, Entamoeba histolytica, and Giardia lamblia","volume":"19","author":"Moldwin","year":"1992","journal-title":"Urol. Clin. N. Am."},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1093\/infdis\/141.2.137","article-title":"Trichomonas vaginalis: Reevaluation of its clinical presentation and laboratory diagnosis","volume":"141","author":"Fouts","year":"1980","journal-title":"J. Infect. Dis."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1666","DOI":"10.12688\/f1000research.19972.1","article-title":"Recent advances in the epidemiology, diagnosis, and management of Trichomonas vaginalis infection","volume":"8","author":"Muzny","year":"2019","journal-title":"F1000Res"},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1128\/CMR.17.4.794-803.2004","article-title":"Trichomoniasis","volume":"17","author":"Schwebke","year":"2004","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_90","first-page":"567","article-title":"InPouch TV culture for detection of Trichomonas vaginalis","volume":"125","author":"Sood","year":"2007","journal-title":"Indian J. Med. Res."},{"key":"ref_91","first-page":"135","article-title":"Trichomonas vaginalis: Diagnosis of a forgotten sexually transmitted infection","volume":"89","year":"2023","journal-title":"An. Real Acad. Farm."},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"3308","DOI":"10.1128\/jcm.35.12.3308-3310.1997","article-title":"Comparison of the InPouch system and Diamond\u00b4s modified medium for detection of Trichomonas vaginalis","volume":"35","author":"Levi","year":"1996","journal-title":"J. Clin. Microbiol."},{"key":"ref_93","first-page":"297","article-title":"A comparison of the sensitivity of the InPouch TV, Diamond\u2019s and Trichosel media for the detection of Trichomonas vaginalis","volume":"73","author":"Borchardt","year":"1997","journal-title":"Genitourin. Med."},{"key":"ref_94","first-page":"10","article-title":"Trichomonas vaginalis: La versatilidad de un par\u00e1sito tenaz","volume":"83","year":"2017","journal-title":"An. Real Acad. Farm."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Mahmoud, A., Sherif, N.A., Abdella, R., El-Genedy, A.R., El Kateb, A.Y., and Askalani, A.N. (2015). Prevalence of Trichomonas vaginalis infection among Egyptian women using culture and latex agglutination: Cross sectional study. BMC Women\u2019s Health, 15.","DOI":"10.1186\/s12905-015-0169-2"},{"key":"ref_96","first-page":"63","article-title":"Development of a latex agglutination test as a simple and rapid method for diagnosis of Trichomonas vaginalis infection","volume":"2","author":"Darani","year":"2010","journal-title":"Avicenna J. Med. Biotechnol."},{"key":"ref_97","first-page":"36600","article-title":"Diagnosis of vaginal candidiasis and Trichomonas vaginalis infection by antibody coated latex particles","volume":"24","author":"Sharafi","year":"2017","journal-title":"J. Adv. Med. Med. Res."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1136\/sti.2003.007864","article-title":"Comparison of latex agglutination, wet preparation, and culture for the detection of Trichomonas vaginalis","volume":"80","author":"Opoku","year":"2004","journal-title":"Sex. Transm. Infect."},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"e12","DOI":"10.1038\/jid.2013.287","article-title":"Enzyme immunoassay and enzyme-linked immunosorbent assay","volume":"133","author":"Gan","year":"2013","journal-title":"J. Investig. Dermatol."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"21","DOI":"10.3347\/kjp.2019.57.1.21","article-title":"Comparison of seropositivity to Trichomonas vaginalis between men with prostatic tumor and normal men","volume":"57","author":"Kim","year":"2019","journal-title":"Korean J. Parasitol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"91","DOI":"10.2147\/ITT.S46694","article-title":"Identification, characterization, and synthesis of peptide epitopes and a recombinant six-epitope protein for Trichomonas vaginalis serodiagnosis","volume":"2","author":"Alderete","year":"2013","journal-title":"ImmunoTargets Ther."},{"key":"ref_102","doi-asserted-by":"crossref","unstructured":"Alderete, J.F. (2020). Advancing prevention of STIs by developing specific serodiagnostic targets: Trichomonas vaginalis as a model. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17165783"},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"e00237","DOI":"10.1016\/j.heliyon.2017.e00237","article-title":"Epitopes within recombinant \u03b1-actinin protein is serodiagnostic target for Trichomonas vaginalis sexually transmitted infections","volume":"3","author":"Alderete","year":"2017","journal-title":"Heliyon"},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"630","DOI":"10.34297\/AJBSR.2021.13.001930","article-title":"Recombinant protein of immunogenic metabolic enzyme epitopes of Trichomonas vaginalis are common to humans and microorganisms","volume":"13","author":"Alderete","year":"2021","journal-title":"Am. J. Biomed. Res."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"742","DOI":"10.24996\/ijs.2020.61.4.5","article-title":"Comparison of trichomoniasis diagnosis by microscopic methods and indirect ELISA technique in a sample of Iraqi women","volume":"61","author":"Bedair","year":"2020","journal-title":"Iraqi J. Sci."},{"key":"ref_106","first-page":"330","article-title":"Evaluation of an enzyme-linked immunosorbent assay for the detection of antibody to Trichomonas vaginalis in sera and vaginal secretions","volume":"58","author":"Street","year":"1982","journal-title":"Br. J. Vener. Dis."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"783","DOI":"10.1128\/CMR.17.4.783-793.2004","article-title":"Treatment of infections caused by metronidazole-resistant Trichomonas vaginalis","volume":"17","author":"Cudmore","year":"2004","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1128\/jcm.24.4.551-555.1986","article-title":"Rapid assay for immunological detection of Trichomonas vaginalis","volume":"24","author":"Watt","year":"1986","journal-title":"J. Clin. Microbiol."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s41182-019-0162-9","article-title":"Prevalence and the evaluation of culture, wet mount, and ELISA methods for the diagnosis of Trichomonas vaginalis infection among Ghanaian women using urine and vaginal specimens","volume":"47","author":"Adjei","year":"2019","journal-title":"Trop. Med. Health"},{"key":"ref_110","doi-asserted-by":"crossref","first-page":"1684","DOI":"10.1128\/jcm.26.9.1684-1686.1988","article-title":"Monoclonal-antibody-based enzyme-linked immunosorbent assay for Trichomonas vaginalis","volume":"26","author":"Lisi","year":"1988","journal-title":"J. Clin. Microbiol."},{"key":"ref_111","doi-asserted-by":"crossref","unstructured":"Zhang, Z., Li, Y., Wang, S., Hao, L., Zhu, Y., Li, H., Song, X., Duan, Y., Sang, Y., and Wu, P. (2020). The molecular characterization and immunity identification of Trichomonas vaginalis adhesion protein 33 (AP33). Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01433"},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1111\/j.1550-7408.1986.tb05557.x","article-title":"An immunofluorescent study of the microtubule organization in Trichomonas vaginalis using antitubulin antibodies","volume":"33","author":"Juliano","year":"1986","journal-title":"J. Protozool."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1128\/jcm.24.6.1107-1108.1986","article-title":"Detection of Trichomonas vaginalis in vaginal specimens by direct immunofluorescence assay","volume":"24","author":"Smith","year":"1986","journal-title":"J. Clin. Microbiol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.ejogrb.2005.07.033","article-title":"Diagnosis of Trichomonas vaginalis infection: The sensitivities and specificities of microscopy, culture and PCR assay","volume":"126","author":"Radonjic","year":"2006","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"9","DOI":"10.4103\/0253-7184.156686","article-title":"Molecular methods in the laboratory diagnosis of sexually transmitted infections","volume":"36","author":"Muralidhar","year":"2015","journal-title":"Indian J. Sex. Transm. Dis. AIDS"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.5858\/arpa.2020-0118-RA","article-title":"Point-of-Care testing for sexually transmitted infections: A review of recent developments","volume":"144","author":"Adamson","year":"2020","journal-title":"Arch. Pathol. Lab. Med."},{"key":"ref_117","doi-asserted-by":"crossref","unstructured":"Alderete, J.F. (2011). Trichomonas vaginalis, reproductive health, and a minority scientist\u2019s experiences. Biol. Reprod., 85.","DOI":"10.1093\/biolreprod\/85.s1.96"},{"key":"ref_118","unstructured":"Sekisui Diagnostics (2023, September 12). Training lesson of OSOM\u00ae Trichomonas IFU. Sekisui Diagnostics. Available online: https:\/\/sekisuidiagnostics.com\/product\/osom-trichomonas-test\/."},{"key":"ref_119","doi-asserted-by":"crossref","unstructured":"Alderete, J.F., and Chan, H. (2023). Poinf-of-Care diagnostic for Trichomonas vaginalis, the most prevalent, non-viral sexually transmitted infection. Pathogens, 12.","DOI":"10.3390\/pathogens12010077"},{"key":"ref_120","unstructured":"Boeras, D., Cohan, J.S., Vojnov, L., Wi, T., Toskin, I., Barrett, K., Ghadreshenas, A., Maatouk, I., and McCartney, D. (2013). The Diagnostic Landscape for Sexually Transmitted Infections, World Health Organization."},{"key":"ref_121","unstructured":"U.S. Food & Drug Administration (2023, September 27). Nucleic Acid Based Test, Available online: https:\/\/www.fda.gov\/medical-devices\/in-vitro-diagnostics\/nucleic-acid-based-tests#microbial."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"e01091-17","DOI":"10.1128\/JCM.01091-17","article-title":"Clinical evaluation of the Cepheid Xpert TV Assay for detection of Trichomonas vaginalis with prospectively collected specimens from men and women","volume":"56","author":"Schwbke","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1586\/erm.11.53","article-title":"APTIMA\u00ae Trichomonas vaginalis, a transcription-mediated amplification assay for detection of Trichomonas vaginalis in urogenital specimens","volume":"11","author":"Chapin","year":"2011","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1128\/JCM.02966-13","article-title":"Detection of Trichomonas vaginalis DNA by use of self-obtained vaginal swabs with the BD ProbeTec Qx assay on the BD viper system","volume":"52","author":"Williams","year":"2014","journal-title":"J. Clin. Micribiol."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1080\/14737159.2020.1842198","article-title":"A profile of the FDA-approved and CE\/IVD-marked Aptima Mycoplasma genitalium assay (Hologic) and key priorities in the management of M. genitalium infections","volume":"20","author":"Shipitsyna","year":"2020","journal-title":"Expert Rev. Mol. Diagn."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"866","DOI":"10.1128\/JCM.02367-10","article-title":"Comparison of Aptima Trichomonas vaginalis transcription-mediated amplification assay and BD affirm VPIII for detection of T. vaginalis in symptomatic women: Performance parameters and epidemiological implications","volume":"49","author":"Andrea","year":"2011","journal-title":"J. Clin. Microbiol."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1097\/OLQ.0000000000001280","article-title":"Clinical performance of the BD CTGCTV2 assay for the BD MAX System for detection of Chlamydia trachomatis, Neisseria gonorrhoeae, and Trichomonas vaginalis infections","volume":"48","author":"Kodsi","year":"2021","journal-title":"Sex. Transm. Dis."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1515\/labmed-2020-0136","article-title":"Simultaneous identification of Chlamydia trachomatis, Neisseria gonorrhoeae, Mycoplasma genitalium, and Trichomonas vaginalis\u2014Multicenter evaluation of the Alinity m STI assay","volume":"45","author":"Goldstein","year":"2021","journal-title":"J. Lab. Med."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"e00332","DOI":"10.1016\/j.plabm.2023.e00332","article-title":"Evaluation of the Abbott Alinity m STI assay for diagnosis of the primary cases of sexually transmitted infections in the United States","volume":"36","author":"Matysiak","year":"2023","journal-title":"Pract. Lab. Med."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1556\/1886.2019.00004","article-title":"Trichomonas vaginalis detection in female specimens with cobas\u00ae TV\/MG for use on the cobas\u00ae 6800\/8800 Systems","volume":"9","author":"Marlowe","year":"2019","journal-title":"Eur. J. Microbiol. Immunol."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1080\/14737159.2017.1282823","article-title":"Clinical performance of the Solana\u00ae Point-of-Care Trichomonas assay from clinician-collected vaginal swabs and urine specimens from symptomatic and asymptomatic women","volume":"17","author":"Gaydos","year":"2017","journal-title":"Exp. Rev. Mol. Diagn."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1097\/OLQ.0000000000000447","article-title":"Rapid diagnosis of Trichomonas vaginalis by testing vaginal swabs in an isothermal helicase-dependent AmpliVue\u2122 Assay","volume":"43","author":"Gaydos","year":"2016","journal-title":"Sex. Transm. Dis."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"668","DOI":"10.1016\/S1473-3099(20)30734-9","article-title":"Performance of a single-use, rapid, point-of-care PCR device for the detection of Neisseria gonorrhoeae, Chlamydia trachomatis, and Trichomonas vaginalis: A cross-sectional study","volume":"21","author":"Morris","year":"2021","journal-title":"Lancet Infect. Dis."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"3197","DOI":"10.1128\/JCM.39.9.3197-3199.2001","article-title":"Evaluation of the Affirm ambient temperature transport system for the detection and identification of Trichomonas vaginalis, Garderella vaginalis, and Candida species from vaginal fluid specimens","volume":"39","author":"Brown","year":"2001","journal-title":"J. Clin. Mirobiol."},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1016\/j.jmoldx.2022.01.010","article-title":"Validation of a new high-throughput BD COR System using the BD CTGCTV2 assay","volume":"24","author":"Taylor","year":"2022","journal-title":"J. Mol. Diagnost."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1136\/sextrans-2016-052902","article-title":"An evaluation study of the Becton-Dickinson ProbeTec Qx (BDQx) Trichomonas vaginalis trichomoniasis molecular diagnostic test in two large, urban STD services","volume":"94","author":"Tipple","year":"2018","journal-title":"Sex. Transm. Infect."},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"e00264-21","DOI":"10.1128\/JCM.00264-21","article-title":"Trichomonas vaginalis detection in urogenital specimens from symptomatic and asymptomatic men and women by use of the cobas TV\/MG Test","volume":"59","author":"Rao","year":"2021","journal-title":"J. Clin. Microbiol."},{"key":"ref_138","doi-asserted-by":"crossref","unstructured":"Bui, H.T.V., Bui, H.T., Chu, S.V., Nguyen, H.T., Nguyen, A.T.V., Truong, P.T., Dang, T.T.H., and Nguyen, A.T.V. (2023). Simultaneous real-time PCR detection of nine prevalent sexually transmitted infections using a predesigned double-quenched TaqMan probe panel. PLoS ONE, 18.","DOI":"10.1371\/journal.pone.0282439"},{"key":"ref_139","doi-asserted-by":"crossref","unstructured":"Hu, X.-M., Xu, J.-X., Jiang, L.-X., Deng, L.-R., Gu, Z.-M., Xie, X.-Y., Ji, H.-C., Wang, W.-H., Li, L.-M., and Tian, C.-N. (2019). Design and evaluation of a novel multiplex Real-Time PCR melting curve assay for the simultaneous detection of nine Sexually Transmitted Disease pathogens in genitourinary secretions. Front. Cell. Infect. Microbiol., 9.","DOI":"10.3389\/fcimb.2019.00382"},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/j.diagmicrobio.2014.03.016","article-title":"Development of a loop-mediated isothermal amplification assay for detection of Trichomonas vaginalis","volume":"79","author":"Reyes","year":"2014","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_141","doi-asserted-by":"crossref","unstructured":"Li, Y., Wang, S., Li, H., Song, X., Zhang, H., Duan, Y., Luo, C., Wang, B., Ji, S., and Xie, Q. (2020). Development of a convenient detection method for Trichomonas vaginalis based on loop-mediated isothermal amplification targeting adhesion protein 65. BMC Infect. Dis., 20.","DOI":"10.1186\/s12879-020-05048-w"},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"1306","DOI":"10.1021\/acsinfecdis.8b00065","article-title":"Aptamer selection against a Trichomonas vaginalis adhesion protein for diagnostic applications","volume":"4","author":"Espiritu","year":"2018","journal-title":"ACS Infect. Dis."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"851635","DOI":"10.3389\/fmed.2022.851635","article-title":"Incidence trends of five common sexually transmitted infections excluding HIV from 1990 to 2019 at the global, regional, and national levels: Results from the global burden of disease study 2019","volume":"9","author":"Fu","year":"2022","journal-title":"Front. Med."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"e611","DOI":"10.1016\/j.ijid.2011.05.006","article-title":"Europe\u2019s neglected infections of poverty","volume":"15","author":"Hotez","year":"2011","journal-title":"Int. J. Infect. Dis."},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"800","DOI":"10.4269\/ajtmh.13-0723","article-title":"Neglected parasitic infections in the United States: Trichomoniasis","volume":"90","author":"Secor","year":"2014","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_146","unstructured":"CDC (2023, October 02). Sexual Risk Behaviours, Available online: https:\/\/www.cdc.gov\/healthyyouth\/sexualbehaviors\/index.htm."},{"key":"ref_147","first-page":"1","article-title":"Sexually transmitted diseases treatment guidelines, 2015","volume":"64","author":"Workowski","year":"2015","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1111\/j.1468-3083.2010.03601.x","article-title":"Guidelines for the laboratory diagnosis of trichomoniasis in East European countries","volume":"24","author":"Domeika","year":"2010","journal-title":"J. Eur. Acad. Dermatol. Venereol."},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"3994","DOI":"10.1128\/JCM.00952-06","article-title":"Methods for detection of Trichomonas vaginalis in male partners or infected women: Implications for control of trichomoniasis","volume":"44","author":"Hobbs","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_150","first-page":"29","article-title":"STI risk perception in the British population and how it relates to sexual behaviour and STI healthcare use: Findings from a cross-sectional survey (Natsal-3)","volume":"2","author":"Clifton","year":"2018","journal-title":"J. Clin. Med."},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.jpag.2015.11.012","article-title":"Age at time of initial sexual intercourse and health of adolescent girls","volume":"29","author":"Lara","year":"2016","journal-title":"J. Pediatr. Adolesc. Gynecol."},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"404","DOI":"10.15698\/mic2016.09.526","article-title":"Trichomoniasis-are we giving the deserved attention to the most common non-viral sexually transmitted disease worldwide?","volume":"3","author":"Menezes","year":"2016","journal-title":"Microb. Cell"},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1097\/01.olq.0000137900.63660.98","article-title":"Estimates of the annual number and cost of new HIV infections among women attributable to trichomoniasis in the United States","volume":"31","author":"Cheeson","year":"2004","journal-title":"Sex. Transm. Dis."},{"key":"ref_154","first-page":"11","article-title":"The estimated direct medical cost of Sexually Transmitted Diseases among American youth, 2000","volume":"35","author":"Chesson","year":"2007","journal-title":"Persp. Sex. Reprod. Health."},{"key":"ref_155","doi-asserted-by":"crossref","first-page":"238","DOI":"10.1097\/OLQ.0000000000001357","article-title":"The estimated lifetime medical cost of Chlamydia, Gonorrhea, and Trichomoniasis in the United States, 2018","volume":"48","author":"Kumar","year":"2021","journal-title":"Sex. Transm. Dis."},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1155\/2005\/373920","article-title":"The laboratory diagnosis of Trichomonas vaginalis","volume":"16","author":"Garber","year":"2005","journal-title":"Can. J. Infect. Dis. Med. Microbiol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/2\/126\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:50:50Z","timestamp":1760104250000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/2\/126"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,29]]},"references-count":156,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["pathogens13020126"],"URL":"https:\/\/doi.org\/10.3390\/pathogens13020126","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,29]]}}}