{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T20:53:17Z","timestamp":1790887997282,"version":"4.1.0"},"reference-count":34,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,11]],"date-time":"2024-02-11T00:00:00Z","timestamp":1707609600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["871029"],"award-info":[{"award-number":["871029"]}]},{"name":"European Union\u2019s Horizon 2020 research and innovation programme","award":["PE00000007"],"award-info":[{"award-number":["PE00000007"]}]},{"name":"EU funding within the NextGenerationEU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases","award":["871029"],"award-info":[{"award-number":["871029"]}]},{"name":"EU funding within the NextGenerationEU-MUR PNRR Extended Partnership initiative on Emerging Infectious Diseases","award":["PE00000007"],"award-info":[{"award-number":["PE00000007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Influenza D virus (IDV) is a novel orthomyxovirus initially isolated from pigs exhibiting influenza-like disease in the USA. Since then, IDV has been detected worldwide in several host species, including livestock animals, whilst specific antibodies have been identified in humans, raising concerns about interspecies transmission and zoonotic risks. Few data regarding the seroprevalence of IDV in small ruminants have been available to date. In this study, we assessed the prevalence of antibodies against IDV in ovine serum samples in Sicily, Southern Italy. Six hundred serum samples, collected from dairy sheep herds located in Sicily in 2022, were tested by haemagglutination inhibition (HI) and virus neutralization (VN) assays using reference strains, D\/660 and D\/OK, representative of two distinct IDV lineages circulating in Italy. Out of 600 tested samples, 168 (28.0%) tested positive to either IDV strain D\/660 or D\/OK or to both by HI whilst 378 (63.0%) tested positive to either IDV strain D\/660 or D\/OK or to both by VN. Overall, our findings demonstrate that IDV circulates in ovine dairy herds in Sicily. Since IDV seems to have a broad host range and it has zoonotic potential, it is important to collect epidemiological information on susceptible species.<\/jats:p>","DOI":"10.3390\/pathogens13020162","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T03:50:27Z","timestamp":1707709827000},"page":"162","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Serological Evidence for Circulation of Influenza D Virus in the Ovine Population in Italy"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4614-677X","authenticated-orcid":false,"given":"Gianvito","family":"Lanave","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, University Aldo Moro of Bari, 70010 Valenzano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Camero","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University Aldo Moro of Bari, 70010 Valenzano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1836-7997","authenticated-orcid":false,"given":"Chiara","family":"Coppola","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6817-2461","authenticated-orcid":false,"given":"Serena","family":"Marchi","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Cascone","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felice","family":"Salina","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2760-8900","authenticated-orcid":false,"given":"Miriana","family":"Coltraro","sequence":"additional","affiliation":[{"name":"Istituto Zooprofilattico Sperimentale della Sicilia \u201cA. Mirri\u201d, 90129 Palermo, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8546-2997","authenticated-orcid":false,"given":"Amienwanlen E.","family":"Odigie","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University Aldo Moro of Bari, 70010 Valenzano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7595-4974","authenticated-orcid":false,"given":"Emanuele","family":"Montomoli","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy"},{"name":"VisMederi S.r.l., 53035 Monteriggioni, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8808-4680","authenticated-orcid":false,"given":"Chiara","family":"Chiapponi","sequence":"additional","affiliation":[{"name":"OIE Reference Laboratory for Swine Influenza, Sede Territoriale di Parma, Istituto Zooprofilattico Sperimentale della Lombardia ed Emilia Romagna, 25124 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincenzo","family":"Cicirelli","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University Aldo Moro of Bari, 70010 Valenzano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5740-6947","authenticated-orcid":false,"given":"Vito","family":"Martella","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, University Aldo Moro of Bari, 70010 Valenzano, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7229-9919","authenticated-orcid":false,"given":"Claudia M.","family":"Trombetta","sequence":"additional","affiliation":[{"name":"Department of Molecular and Developmental Medicine, University of Siena, 53100 Siena, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1146\/annurev.pathmechdis.3.121806.154316","article-title":"The pathology of influenza virus infections","volume":"3","author":"Taubenberger","year":"2008","journal-title":"Annu. Rev. Pathol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Hause, B.M., Ducatez, M., Collin, E.A., Ran, Z., Liu, R., Sheng, Z., Armien, A., Kaplan, B., Chakravarty, S., and Hoppe, A.D. (2013). Isolation of a novel swine influenza virus from Oklahoma in 2011 which is distantly related to human influenza C viruses. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003176"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e00031-14","DOI":"10.1128\/mBio.00031-14","article-title":"Characterization of a novel influenza virus in cattle and Swine\/proposal for a new genus in the Orthomyxoviridae family","volume":"5","author":"Hause","year":"2014","journal-title":"mBio"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1580","DOI":"10.1080\/21505594.2017.1365216","article-title":"Novel influenza D virus\/Epidemiology, pathology, evolution and biological characteristics","volume":"8","author":"Su","year":"2017","journal-title":"Virulence"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"11660","DOI":"10.1038\/s41598-017-12012-3","article-title":"Influenza D in Italy: Towards a better understanding of an emerging viral infection in swine","volume":"7","author":"Foni","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1020","DOI":"10.3201\/eid2406.172102","article-title":"Influenza D Virus Infection in Feral Swine Populations, United States","volume":"24","author":"Ferguson","year":"2018","journal-title":"Emerg. Infect. Dis."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Gorin, S., Fablet, C., Qu\u00e9guiner, S., Barbier, N., Paboeuf, F., Herv\u00e9, S., Rose, N., and Simon, G. (2019). Assessment of Influenza D Virus in Domestic Pigs and Wild Boars in France: Apparent Limited Spread within Swine Populations Despite Serological Evidence of Breeding Sow Exposure. Viruses., 12.","DOI":"10.3390\/v12010025"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1224","DOI":"10.3201\/eid2506.181158","article-title":"Influenza D Virus Infection in Dromedary Camels, Ethiopia","volume":"25","author":"Murakami","year":"2019","journal-title":"Emerg. Infect. Dis."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1186\/s13620-019-0150-8","article-title":"Seroprevalence of influenza D virus in selected sample groups of Irish cattle, sheep and pigs","volume":"72","author":"Donohoe","year":"2019","journal-title":"Ir. Vet. J."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Oliva, J., Eichenbaum, A., Belin, J., Gaudino, M., Guillotin, J., Alzieu, J.P., Nicollet, P., Brugidou, R., Gueneau, E., and Michel, E. (2019). Serological Evidence of Influenza D Virus Circulation Among Cattle and Small Ruminants in France. Viruses, 11.","DOI":"10.3390\/v11060516"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.vetmic.2015.09.005","article-title":"Serological evidence for the presence of influenza D virus in small ruminants","volume":"180","author":"Quast","year":"2015","journal-title":"Vet. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"e148","DOI":"10.1111\/zph.12423","article-title":"Serological evidence for the co-circulation of two lineages of influenza D viruses in equine populations of the Midwest United States","volume":"65","author":"Nedland","year":"2018","journal-title":"Zoonoses Public Health"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Trombetta, C.M., Marchi, S., Manini, I., Kistner, O., Li, F., Piu, P., Manenti, A., Biuso, F., Sreenivasan, C., and Druce, J. (2019). Influenza D Virus: Serological Evidence in the Italian Population from 2005 to 2017. Viruses, 12.","DOI":"10.3390\/v12010030"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2855","DOI":"10.1002\/jmv.27466","article-title":"Detection of antibodies against influenza D virus in swine veterinarians in Italy in 2004","volume":"94","author":"Trombetta","year":"2022","journal-title":"J. Med. Virol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.jcv.2016.05.017","article-title":"Serologic evidence of exposure to influenza d virus among persons with occupational contact with cattle","volume":"81","author":"White","year":"2016","journal-title":"J. Clin. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1125","DOI":"10.1111\/tbed.13812","article-title":"Emerging Influenza D virus infection in European livestock as determined in serology studies: Are we underestimating its spread over the continent?","volume":"68","author":"Gaudino","year":"2021","journal-title":"Transbound Emerg. Dis."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e02084-17","DOI":"10.1128\/JVI.02084-17","article-title":"Influenza C and D viruses package eight organized ribonucleoprotein complexes","volume":"92","author":"Nakatsu","year":"2018","journal-title":"J. Virol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1080\/22221751.2021.1910078","article-title":"Emergence of new phylogenetic lineage of Influenza D virus with broad antigenicity in California, United States","volume":"10","author":"Huang","year":"2021","journal-title":"Emerg. Microbes Infect."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"105769","DOI":"10.1016\/j.micpath.2022.105769","article-title":"Recent strains of influenza D virus create a new genetic cluster for European strains","volume":"172","author":"Yesilbag","year":"2022","journal-title":"Microb. Pathog."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Chiapponi, C., Faccini, S., Fusaro, A., Moreno, A., Prosperi, A., Merenda, M., Baioni, L., Gabbi, V., Rosignoli, C., and Alborali, G.L. (2019). Detection of a New Genetic Cluster of Influenza D Virus in Italian Cattle. Viruses, 11.","DOI":"10.3390\/v11121110"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1036","DOI":"10.1128\/JVI.02718-14","article-title":"Cocirculation of two distinct genetic and antigenic lineages of proposed influenza D virus in cattle","volume":"89","author":"Collin","year":"2015","journal-title":"J. Virol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Mazzetto, E., Bortolami, A., Fusaro, A., Mazzacan, E., Maniero, S., Vascellari, M., Beato, M.S., Schiavon, E., Chiapponi, C., and Terregino, C. (2020). Replication of Influenza D Viruses of Bovine and Swine Origin in Ovine Respiratory Explants and Their Attachment to the Respiratory Tract of Bovine, Sheep, Goat, Horse, and Swine. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.01136"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Kwasnik, M., Rola, J., and Rozek, W. (2023). Influenza D in Domestic and Wild Animals. Viruses, 15.","DOI":"10.3390\/v15122433"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1556","DOI":"10.3201\/eid2309.170342","article-title":"Serologic Evidence for Influenza C and D Virus among Ruminants and Camelids, Africa, 1991\u20132015","volume":"23","author":"Salem","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Sanogo, I.N., Kouakou, C., Batawui, K., Djegui, F., Byarugaba, D.K., Adjin, R., Adjabli, K., Wabwire-Mangen, F., Erima, B., and Atim, G. (2021). Serological Surveillance of Influenza D Virus in Ruminants and Swine in West and East Africa, 2017\u20132020. Viruses, 13.","DOI":"10.3390\/v13091749"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.3201\/eid2308.170059","article-title":"Influenza D Virus in Animal Species in Guangdong Province, Southern China","volume":"23","author":"Zhai","year":"2017","journal-title":"Emerg. Infect. Dis."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Tumino, S., Tolone, M., Galluzzo, P., Migliore, S., Sechi, T., Bordonaro, S., Puleio, R., Carta, A., and Loria, G.R. (2022). Alternative Molecular Tools for the Fight against Infectious Diseases of Small Ruminants\/Native Sicilian Sheep Breeds and Maedi-Visna Genetic Susceptibility. Animals, 12.","DOI":"10.3390\/ani12131630"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1093\/biomet\/26.4.404","article-title":"The use of confidence or fiducial limits illustrated in the case of the binomial","volume":"26","author":"Clopper","year":"1934","journal-title":"Biometrika"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"452","DOI":"10.1038\/bmt.2012.244","article-title":"Investigation of the freely available easy-to-use software \u2018EZR\u2019 for medical statistics","volume":"48","author":"Kanda","year":"2013","journal-title":"Bone Marrow Transpl."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1392","DOI":"10.1111\/tbed.13805","article-title":"Formal estimation of the seropositivity cut-off of the hemagglutination inhibition assay in field diagnosis of influenza D virus in cattle and estimation of the associated true prevalence in Morocco","volume":"68","author":"Saegerman","year":"2021","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"veac081","DOI":"10.1093\/ve\/veac081","article-title":"Evolutionary and temporal dynamics of emerging influenza D virus in Europe (2009-22)","volume":"8","author":"Gaudino","year":"2022","journal-title":"Virus Evol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1126\/science.94.2427.22","article-title":"The agglutination of red cells by allantoic fluid of chick embryos infected with influenza virus","volume":"94","author":"Hirst","year":"1941","journal-title":"Science"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.jcv.2019.08.009","article-title":"Influenza virus-specific CD4+ and CD8+ T cell-mediated immunity induced by infection and vaccination","volume":"119","author":"Jansen","year":"2019","journal-title":"J. Clin. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"519","DOI":"10.1586\/erv.13.35","article-title":"Immunological assessment of influenza vaccines and immune correlates of protection","volume":"12","author":"Reber","year":"2013","journal-title":"Expert Rev. Vaccines"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/2\/162\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T13:58:38Z","timestamp":1760104718000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/2\/162"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,11]]},"references-count":34,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["pathogens13020162"],"URL":"https:\/\/doi.org\/10.3390\/pathogens13020162","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,11]]}}}