{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:23:43Z","timestamp":1792790623112,"version":"4.5.0"},"reference-count":63,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T00:00:00Z","timestamp":1707955200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Due to its adaptive versatility to numerous types of habitats, extremely diverse both in terms of composition and specificity, developed in various areas of the Western Plains of Romania, the European fallow deer (Dama dama) is a species with high ecological plasticity. In this area, the D. dama interacts with other species of wild fauna but also with numerous domestic animals, an important aspect in terms of the sanitary-veterinary status of animal populations, as well as the existence of a potential risk of infection with various species of parasites that can cause the D. dama specimens to obtain certain diseases and even zoonoses. A total of 133 esophagi from D. dama have been examined for helminths. Of the 133 esophagus samples from D. dama, nematodes of the genus Gongylonema were identified in 25 (18.80%). Sequencing revealed that the nematode identified in the samples was 99% similar to the sequence of Gongylonema pulchrum (GenBank no. LC026018.1, LC388754.1, AB646061). The present research is the first report of the nematode G. pulchrum from D. dama in Romania.<\/jats:p>","DOI":"10.3390\/pathogens13020175","type":"journal-article","created":{"date-parts":[[2024,2,15]],"date-time":"2024-02-15T05:55:59Z","timestamp":1707976559000},"page":"175","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["First Molecular Data of Gongylonema pulchrum (Rhabditida: Gongylonematidae) in European Fallow Deer Dama dama from Romania"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-8535-3434","authenticated-orcid":false,"given":"Dan-Cornel","family":"Popovici","sequence":"first","affiliation":[{"name":"Forestry Faculty, Transilvania University Brasov, 500123 Brasov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana-Maria","family":"Marin","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ovidiu","family":"Ionescu","sequence":"additional","affiliation":[{"name":"Forestry Faculty, Transilvania University Brasov, 500123 Brasov, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Monica Florina","family":"Moraru","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Durmu\u0219 Alpaslan","family":"Kaya","sequence":"additional","affiliation":[{"name":"Faculty of Agriculture, Hatay Mustafa Kemal University, Hatay 31060, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3004-463X","authenticated-orcid":false,"given":"Mirela","family":"Imre","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Narcisa","family":"Mederle","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, University of Life Sciences \u201cKing Michael I\u201d from Timisoara, 300645 Timisoara, Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,2,15]]},"reference":[{"key":"ref_1","unstructured":"Chapman, D., and Chapman, N. (1975). Fallow Deer Their History, Distribution and Biology, Terence Dalton Lavenham."},{"key":"ref_2","first-page":"206","article-title":"Angaben zum friihholoz\u0103nen Vorkommen des Damhirschen Cervus (Dama) dama L. 1758 in Europa","volume":"19","author":"Bokonyi","year":"1971","journal-title":"Saugetier Wiss. Mitteilungen"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Samuel, W.M., Pybus, M.J., and Kocan, A.A. (2001). Parasitic Diseases of Wild Mammals, Iowa State University Press.","DOI":"10.1002\/9780470377000"},{"key":"ref_4","first-page":"63","article-title":"Parasitic helminths of domesticated and wild ruminants in Cholistan Desert of Pakistan","volume":"14","author":"Farooq","year":"2012","journal-title":"Int. J Agric. Biol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1515\/bvip-2015-0050","article-title":"First report of the giant liver fluke (Fascioloides magna) infection in farmed fallow deer (Dama dama) in Poland\u2014Pathomorphological changes and molecular identification","volume":"59","author":"Karamon","year":"2015","journal-title":"Bull. Vet. Inst. Pulawy"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/j.ijppaw.2021.05.006","article-title":"First record of chronic Fascioloides magna infection in roe deer (Capreolus capreolus)","volume":"15","author":"Beck","year":"2021","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1381","DOI":"10.1002\/ece3.1414","article-title":"Life history and biology of Fascioloides magna (Trematoda) and its native and exotic hosts","volume":"5","author":"Malcicka","year":"2015","journal-title":"Ecol. Evol."},{"key":"ref_8","first-page":"11","article-title":"Gastrointestinal parasites and lung worms of wild ruminants from southwestern Bulgaria. i. Cervidae: Red deer (Cervus elaphus l. 1758) and fallow deer (Dama dama L. 1758)","volume":"7","author":"Radev","year":"2022","journal-title":"Tradit. Mod. Vet. Med."},{"key":"ref_9","first-page":"1","article-title":"The researches regarding digestive parasitism in herbivorous from Zoological Garden Timisoara","volume":"42","author":"Afrenie","year":"2009","journal-title":"Actual. Anim. Breed. Pathol."},{"key":"ref_10","first-page":"75","article-title":"Frequency of gastrointestinal and pulmonary helminth infections in wild deer from western Romania","volume":"8","author":"Hora","year":"2017","journal-title":"Vet. Parasitol. Reg. Stud. Rep."},{"key":"ref_11","first-page":"89","article-title":"Study regarding the infestation with gastrointestinal nematodes in fallow deer (Dama dama L.) from Timi\u0219 county","volume":"56","author":"Popovici","year":"2023","journal-title":"Lucr. \u015etiin\u0163ifice Med. Vet. Timi\u0219."},{"key":"ref_12","first-page":"213","article-title":"Evaluation of endoparasitism in fallow deer (Dama dama L.) from Bihor county (Romania) hunting grounds","volume":"56","author":"Popovici","year":"2023","journal-title":"Lucr. \u015etiin\u0163ifice Med. Vet. Timi\u0219."},{"key":"ref_13","first-page":"172","article-title":"Evaluation of the parasitic load in fallow deer (Dama dama L.) from Arad County","volume":"55","author":"Popovici","year":"2022","journal-title":"Lucr. \u015etiin\u0163ifice Med. Vet. Timi\u0219."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"311","DOI":"10.4274\/tpd.galenos.2021.77486","article-title":"Literature Review of Gongylonema pulchrum: A Rare Nematode","volume":"45","year":"2021","journal-title":"Turk. Parazitol. Derg."},{"key":"ref_15","first-page":"37","article-title":"Prevalence and pathology of Gongylonema pulchrum in cattle slaughtered in Rudsar, northern Iran","volume":"14","author":"Kheirandish","year":"2013","journal-title":"Sci. Parasitol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"734","DOI":"10.1016\/j.asjsur.2023.10.012","article-title":"Gongylonema pulchrum infection in human esophagus: A case report","volume":"47","author":"Li","year":"2024","journal-title":"Asian J. Surg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.oooo.2017.11.019","article-title":"Gongylonema pulchrum infection in the human oral cavity: A case report and literature review","volume":"125","author":"Liu","year":"2018","journal-title":"Oral Surg. Oral Med. Oral Pathol. Oral Radiol."},{"key":"ref_18","first-page":"38","article-title":"Contributii la studiul helmintofaunei caprioarei (Capreolus capreolus) in R.P.R","volume":"7","author":"Stoican","year":"1959","journal-title":"Probl. Parazitol. Vet."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1638\/2018-0040","article-title":"Prevalence and intensity of digestive and pulmonary parasites in wild boars in Romania","volume":"50","author":"Hora","year":"2019","journal-title":"J. Zoo Wildl. Med."},{"key":"ref_20","unstructured":"Rusu, \u015e., Erhan, D., Toderash, I., Zamornea, M., Chihai, O., Rusu, V., Gologan, I., Bondari, L., and Ghenciu, V. (2022, January 24\u201325). Innovative method of deworming and complementary feeding of wild boars. Proceedings of the Biology and Sustainable Development, Bac\u0103u, Romania."},{"key":"ref_21","unstructured":"(2023, August 11). Ordinul Ministrului Mediului, Apelor \u0219i P\u0103durilor Nr. 1571\/07.06.2022 Privind Aprobarea Cotelor de Recolt\u0103 Pentru Unele specii de Faun\u0103 de Interes Cinegetic. Available online: https:\/\/mmediu.ro\/articol\/ordinul-ministrului-mediului-apelor-si-padurilor-nr-1571."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1016\/j.vetpar.2006.04.012","article-title":"Differential diagnosis of dog hookworms based on PCR-RFLP from the ITS region of their rDNA","volume":"140","author":"Miranda","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2525","DOI":"10.1093\/nar\/21.10.2525","article-title":"Rapid Sequencing of rDNA from Single Worms and Eggs of Parasitic Helminths","volume":"21","author":"Gasse","year":"1993","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1093\/nar\/gkn180","article-title":"Phylogeny.fr: Robust phylogenetic analysis for the non-specialist","volume":"36","author":"Deeeper","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e13","DOI":"10.1017\/S0022149X18001001","article-title":"Gongylonema infection of wild mammals in Japan and Sardinia (Italy)","volume":"94","author":"Setsuda","year":"2018","journal-title":"J. Helminthol."},{"key":"ref_26","unstructured":"Soulsby, E., and Helminthes, J.L. (1982). Arthropods and Protozoa of Domesticated Animals, Bailliere Tindall. [7th ed.]."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1016\/S0304-4017(03)00211-5","article-title":"Wild boar helminths: Risks in animal translocations","volume":"115","author":"Gortazar","year":"2003","journal-title":"Vet. Parasitol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"252","DOI":"10.7589\/0090-3558-25.2.252","article-title":"Helminth parasites of intermingling axis deer, wild swine and domestic cattle from the island of Molokai, Hawaii","volume":"25","author":"McKenzie","year":"1989","journal-title":"J. Wildl. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"264","DOI":"10.7589\/0090-3558-21.3.264","article-title":"Effects of season and physical condition on the gastrointestinal helminth community of white-tailed deer from the Texas Edwards Plateau","volume":"21","author":"Waid","year":"1985","journal-title":"J. Wildl. Dis."},{"key":"ref_30","first-page":"416","article-title":"Parasitic Helminths in Wild Boars (Sus scrofa) in Mazandaran Province, Northern Iran","volume":"13","author":"Dodangeh","year":"2018","journal-title":"Iran J. Parasitol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1017\/S0022149X1000074X","article-title":"Helminth infections of wild boars (Sus scrofa) in the Bursa province of Turkey","volume":"85","author":"Senlik","year":"2011","journal-title":"J. Helminthol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1017\/S0022149X12000442","article-title":"Genetic variation of Gongylonema pulchrum from wild animals and cattle in Japan based on ribosomal RNA and mitochondrial cytochrome c oxidase subunit I genes","volume":"87","author":"Makouloutou","year":"2013","journal-title":"J. Helminthol."},{"key":"ref_33","first-page":"45","article-title":"Study on the prevalence and pathology of Gongylonema pulchrum (gullet worm) of sheep from Iran","volume":"5","author":"Eslami","year":"2010","journal-title":"Glob. Vet."},{"key":"ref_34","first-page":"166","article-title":"A study on prevalence, morphology and morphometric of Gongylonema pulchrum in sheep slaughtered in Sari, Northern Iran","volume":"10","author":"Galeh","year":"2023","journal-title":"Int. J. Med. Lab."},{"key":"ref_35","first-page":"56","article-title":"Esophageal gongylonemosis in ruminants slaughtered in Hamedan and Babol, Iran","volume":"4","author":"Sazmand","year":"2020","journal-title":"J. Zoonotic Dis."},{"key":"ref_36","first-page":"525","article-title":"Fauna and distribution of nematodes from the suborders spirurata and filariata parasitizing cattle in Dagestan, from the perspective of vertical zoning","volume":"44","author":"Zubairova","year":"2010","journal-title":"Parazitologiia"},{"key":"ref_37","first-page":"20","article-title":"The prevalence of gastro-intestinal nematodes in hair goats of the Sanliurfa region Turkiye","volume":"33","author":"Sevgili","year":"2009","journal-title":"Parazitol. Derg."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"123","DOI":"10.2307\/3277467","article-title":"Geographic distribution of Gongylonema pulchrum, Gongylonema verrucosum, and Paramphistomum liorchis in white-tailed deer of the southeastern United States","volume":"56","author":"Prestwood","year":"1970","journal-title":"J. Parasitol."},{"key":"ref_39","first-page":"4514","article-title":"First report of the wild Tibetan macaque (Macaca thibetana) as a new primate host of Gongylonema pulchrum with high incidence in China","volume":"11","author":"Yong","year":"2012","journal-title":"J. Anim. Vet. Adv."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.vetpar.2018.06.023","article-title":"First survey on zoonotic helminthosis in urban brown rats (Rattus norvegicus) in Spain and associated public health considerations","volume":"259","author":"Pascual","year":"2018","journal-title":"Vet. Parasitol."},{"key":"ref_41","first-page":"84","article-title":"Oesophageal and gastric gongylonemiasis in a donkey","volume":"9","author":"Movassaghi","year":"2008","journal-title":"Iran. J. Vet. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"119","DOI":"10.2478\/s11686-006-0018-4","article-title":"Natural infection of Oryctolagus cuniculus (Lagomorpha, Leporidae) by Gongylonema neoplasticum (Nematoda, Gongylonematidae) in Portugal","volume":"51","author":"Eira","year":"2006","journal-title":"Acta Parasitol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"528","DOI":"10.7589\/JWD-D-22-00108","article-title":"Gongylonema sp. in the Tongue of a Brown-Nosed Coati (Nasua nasua) from the Brazilian Atlantic","volume":"59","author":"Soares","year":"2023","journal-title":"For. J. Wildl. Dis."},{"key":"ref_44","first-page":"31","article-title":"Biology and transmission of the gullet worm (Gongylonema pulchrum Molin, 1857)","volume":"36","author":"Sato","year":"2009","journal-title":"Yamaguchi J. Vet. Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1007\/s00436-015-4806-3","article-title":"Intraspecific and interspecific genetic variation of Gongylonema pulchrum and two rodent Gongylonema spp. (G. aegypti and G. neoplasticum), with the proposal of G. nepalensis n. sp. for the isolate in water buffaloes from Nepal","volume":"115","author":"Setsuda","year":"2016","journal-title":"Parasitol. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1016\/j.vetpar.2017.08.022","article-title":"First record of Gongylonema nepalensis in domestic and wild ruminants in Europe","volume":"246","author":"Varcasia","year":"2017","journal-title":"Vet. Parasitol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1654\/4767.1","article-title":"A Survey of Nematode Parasites of Small Mammals in Tunisia, North Africa: Diversity of Species and Zoonotic Implications","volume":"82","author":"Jrijer","year":"2015","journal-title":"Comp. Parasitol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"80","DOI":"10.2307\/3271023","article-title":"The life history of Gongylonema scutatum","volume":"2","author":"Ransom","year":"1915","journal-title":"J. Parasitol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"129","DOI":"10.33988\/auvfd.710010","article-title":"Prevalence and molecular diagnosis of Gongylonema pulchrum in cattle and sheep in the Samsun region Ankara","volume":"68","author":"Umur","year":"2021","journal-title":"Univ. Vet. Fak. Derg."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1016\/j.parint.2013.07.005","article-title":"Gongylonema sp. infection in the scops owl (Otus scops)","volume":"62","author":"Lopes","year":"2013","journal-title":"Parasitol. Int."},{"key":"ref_51","first-page":"294","article-title":"Disease prevalence in an urban raptor related to pest species: The case of Eurasian Scops Owl Otus scops infection by Gongylonema sp.","volume":"166","author":"Lopes","year":"2024","journal-title":"Int. J. Avian Sci."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"102443","DOI":"10.1016\/j.parint.2021.102443","article-title":"Identification of the intermediate host of Gongylonema sp., the etiological agent of the necrotic oropharyngeal disease of the Scops owl (Otus scops)","volume":"86","author":"Lopes","year":"2022","journal-title":"Parasitol. Int."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1082","DOI":"10.1001\/2013.jamaoto.386","article-title":"Gongylonema: A parasitic nematode of the oral cavity","volume":"138","author":"Ayala","year":"2012","journal-title":"Arch. Otolaryngol. Head Neck Surg."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1051\/parasite\/2013007","article-title":"First case of human gongylonemosis in France","volume":"20","author":"Pesson","year":"2013","journal-title":"Parasite"},{"key":"ref_55","first-page":"e62","article-title":"First case of human Gongylonema pulchrum infection in Slovenia","volume":"22","author":"Kramar","year":"2019","journal-title":"J. Helminthol."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Zhou, O., Wei, Y., Zhai, H., Li, S., Xu, R., and Li, P. (2021). Comorbid early esophageal cancer and Gongylonema pulchrum infection: A case report. BMC Gastroenterol., 21.","DOI":"10.1186\/s12876-021-01873-8"},{"key":"ref_57","first-page":"595","article-title":"Intraocular Gongylonema Infection: First Case in Humans","volume":"26","author":"Waisberg","year":"2018","journal-title":"Ocul. Immunol. Inflamm."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"15","DOI":"10.2478\/s11687-013-0102-x","article-title":"Endoparasitoses of fallow deer (Dama dama) in game-park in South Moravia (Czech Republic)","volume":"50","author":"Borkovcova","year":"2013","journal-title":"Helminthologia"},{"key":"ref_59","first-page":"1543","article-title":"The impact of habitat on distribuion of giant liver fluke (Fascioloides magna) in Eastern Croatia","volume":"36","author":"Florijancic","year":"2008","journal-title":"Cereal Res. Commun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"13","DOI":"10.3347\/kjp.2009.47.1.13","article-title":"A Survey of Dung Beetles Infected with Larval Nematodes with Particular Note on Copris lunaris Beetles as a Vector for Gongylonema sp. in Iran","volume":"47","author":"Mowlavi","year":"2009","journal-title":"Korean J. Parasitol."},{"key":"ref_61","first-page":"99","article-title":"Intermediate hosts of Gongylonema pulchrum Molin, 1857 (Nematoda, Gongylonematidae) in game farm pastures","volume":"38","author":"Todev","year":"2001","journal-title":"Helminthologia"},{"key":"ref_62","first-page":"10","article-title":"Incidence and genetic characterization of Gongylonema pulchrum in cattle slaughtered in Mazandaran Province, Northern Iran","volume":"5","author":"Halajian","year":"2010","journal-title":"Iran. J. Parasitol."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Yang, J., Okyere, S.K., Zheng, J., Cao, B., and Hu, Y. (2022). Seasonal Prevalence of Gastrointestinal Parasites in Macaques (Macaca thibetana) at Mount Emei Scenic Area in China. Animals, 12.","DOI":"10.3390\/ani12141816"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/2\/175\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:00:00Z","timestamp":1760104800000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/2\/175"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,15]]},"references-count":63,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2024,2]]}},"alternative-id":["pathogens13020175"],"URL":"https:\/\/doi.org\/10.3390\/pathogens13020175","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,2,15]]}}}