{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T11:04:52Z","timestamp":1785150292626,"version":"3.55.0"},"reference-count":101,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T00:00:00Z","timestamp":1709596800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Xylella fastidiosa (Xf) is a global bacterial threat for a diversity of plants, including olive trees. However, current understanding of host responses upon Xf-infection is limited to allow early disease prediction, diagnosis, and sustainable strategies for breeding on plant tolerance. Recently, we identified a major complex trait for early de novo programming, named CoV-MAC-TED, by comparing early transcriptome data during plant cell survival with SARS-CoV-2-infected human cells. This trait linked ROS\/RNS balancing during first hours of stress perception with increased aerobic fermentation connected to alpha-tubulin-based cell restructuration and control of cell cycle progression. Furthermore, our group had advanced concepts and strategies for breeding on plant holobionts. Here, we studied tolerance against Xf-infection by applying a CoV-MAC-TED-related gene set to (1) progress proof-of-principles, (2) highlight the importance of individual host responses for knowledge gain, (3) benefit sustainable production of Xf-threatened olive, (4) stimulate new thinking on principle roles of secondary metabolite synthesis and microbiota for system equilibration and, (5) advance functional marker development for resilience prediction including tolerance to Xf-infections. We performed hypothesis-driven complex analyses in an open access transcriptome of primary target xylem tissues of naturally Xf-infected olive trees of the Xf-tolerant cv. Leccino and the Xf-susceptible cv. Ogliarola. The results indicated that cyanide-mediated equilibration of oxygen-dependent respiration and carbon-stress alleviation by the help of increased glycolysis-driven aerobic fermentation paths and phenolic metabolism associate to tolerance against Xf. Furthermore, enhanced alternative oxidase (AOX) transcript levels through transcription Gleichschaltung linked to quinic acid synthesis appeared as promising trait for functional marker development. Moreover, the results support the idea that fungal endophytes strengthen Xf-susceptible genotypes, which lack efficient AOX functionality. Overall, this proof-of-principles approach supports the idea that efficient regulation of the multi-functional AOX gene family can assist selection on multiple-resilience, which integrates Xf-tolerance, and stimulates future validation across diverse systems.<\/jats:p>","DOI":"10.3390\/pathogens13030227","type":"journal-article","created":{"date-parts":[[2024,3,5]],"date-time":"2024-03-05T05:31:19Z","timestamp":1709616679000},"page":"227","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Transcriptome Analyses in Adult Olive Trees Indicate Acetaldehyde Release and Cyanide-Mediated Respiration Traits as Critical for Tolerance against Xylella fastidiosa and Suggest AOX Gene Family as Marker for Multiple-Resilience"],"prefix":"10.3390","volume":"13","author":[{"given":"Birgit","family":"Arnholdt-Schmitt","sequence":"first","affiliation":[{"name":"Non-Institutional Competence Focus (NICFocus) \u2018Functional Cell Reprogramming and Organism Plasticity\u2019 (FunCROP), Coordinated from Foros de Vale de Figueira, 7050-704 Alentejo, Portugal"},{"name":"Functional Genomics and Bioinformatics Group, Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debabrata","family":"Sircar","sequence":"additional","affiliation":[{"name":"Non-Institutional Competence Focus (NICFocus) \u2018Functional Cell Reprogramming and Organism Plasticity\u2019 (FunCROP), Coordinated from Foros de Vale de Figueira, 7050-704 Alentejo, Portugal"},{"name":"Biosciences and Bioengineering Department, Indian Institute of Technology Roorkee, Roorkee 247667, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4657-1066","authenticated-orcid":false,"given":"Shahid","family":"Aziz","sequence":"additional","affiliation":[{"name":"Non-Institutional Competence Focus (NICFocus) \u2018Functional Cell Reprogramming and Organism Plasticity\u2019 (FunCROP), Coordinated from Foros de Vale de Figueira, 7050-704 Alentejo, Portugal"},{"name":"Functional Genomics and Bioinformatics Group, Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thais Andrade","family":"Germano","sequence":"additional","affiliation":[{"name":"Non-Institutional Competence Focus (NICFocus) \u2018Functional Cell Reprogramming and Organism Plasticity\u2019 (FunCROP), Coordinated from Foros de Vale de Figueira, 7050-704 Alentejo, Portugal"},{"name":"Functional Genomics and Bioinformatics Group, Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine Leit\u00e3o Lima","family":"Thiers","sequence":"additional","affiliation":[{"name":"Non-Institutional Competence Focus (NICFocus) \u2018Functional Cell Reprogramming and Organism Plasticity\u2019 (FunCROP), Coordinated from Foros de Vale de Figueira, 7050-704 Alentejo, Portugal"},{"name":"Functional Genomics and Bioinformatics Group, Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6633-2698","authenticated-orcid":false,"given":"Carlos","family":"Noceda","sequence":"additional","affiliation":[{"name":"Non-Institutional Competence Focus (NICFocus) \u2018Functional Cell Reprogramming and Organism Plasticity\u2019 (FunCROP), Coordinated from Foros de Vale de Figueira, 7050-704 Alentejo, Portugal"},{"name":"Cell and Molecular Biotechnology of Plants (BIOCEMP)\/Industrial Biotechnology and Bioproducts, Departamento de Ciencias de la Vida y de la Agricultura, Universidad de las Fuerzas Armadas-ESPE, Sangolqu\u00ed 171103, Ecuador"},{"name":"Facultad de Ingenier\u00eda, Universidad Estatal de Milagro (UNEMI), Guayas 091050, Ecuador"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Revuru","family":"Bharadwaj","sequence":"additional","affiliation":[{"name":"Non-Institutional Competence Focus (NICFocus) \u2018Functional Cell Reprogramming and Organism Plasticity\u2019 (FunCROP), Coordinated from Foros de Vale de Figueira, 7050-704 Alentejo, Portugal"},{"name":"Biosciences and Bioengineering Department, Indian Institute of Technology Roorkee, Roorkee 247667, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gunasekaran","family":"Mohanapriya","sequence":"additional","affiliation":[{"name":"Non-Institutional Competence Focus (NICFocus) \u2018Functional Cell Reprogramming and Organism Plasticity\u2019 (FunCROP), Coordinated from Foros de Vale de Figueira, 7050-704 Alentejo, Portugal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7914-0150","authenticated-orcid":false,"given":"Jos\u00e9 H\u00e9lio","family":"Costa","sequence":"additional","affiliation":[{"name":"Non-Institutional Competence Focus (NICFocus) \u2018Functional Cell Reprogramming and Organism Plasticity\u2019 (FunCROP), Coordinated from Foros de Vale de Figueira, 7050-704 Alentejo, Portugal"},{"name":"Functional Genomics and Bioinformatics Group, Department of Biochemistry and Molecular Biology, Federal University of Cear\u00e1, Fortaleza 60451-970, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,3,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Quetglas, B., Olmo, D., Nieto, A., Borr\u00e0s, D., Adrover, F., Pedrosa, A., Montesinos, M., de Dios Garc\u00eda, J., L\u00f3pez, M., and Juan, A. 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