{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T02:41:24Z","timestamp":1784860884609,"version":"3.55.0"},"reference-count":41,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T00:00:00Z","timestamp":1719964800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Education, Research, Development and Youth of the Slovak Republic","award":["VEGA 1\/0569\/24"],"award-info":[{"award-number":["VEGA 1\/0569\/24"]}]},{"name":"Ministry of Education, Research, Development and Youth of the Slovak Republic","award":["VEGA 2\/0014\/21"],"award-info":[{"award-number":["VEGA 2\/0014\/21"]}]},{"name":"Ministry of Education, Research, Development and Youth of the Slovak Republic","award":["VEGA 1\/0177\/22"],"award-info":[{"award-number":["VEGA 1\/0177\/22"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Babesia gibsoni is a parasitic protozoan transmitted through tick bites and can cause severe disease in dogs. It can also be transmitted through direct contact with infected blood during dog fights, blood transfusions, and from dam to offspring during the perinatal period, resulting in stillborn or dead newborn puppies. This study aimed to determine the incidence of infection, the viability of newborn puppies, and the degree of B. gibsoni transmission from infected dam to offspring during pregnancy and lactation. Using PCR-based molecular methods, B. gibsoni infection in a pregnant American Pit Bull Terrier and her newborn puppies was confirmed. The incidence of B. gibsoni infection in the litter reached 75%. Out of eight puppies, six were infected with B. gibsoni, and one died. A therapeutic protocol comprising Malarone\u00ae, azithromycin, and artesunate was administered to a lactating B. gibsoni-positive bitch. By day 77 after birth, three out of five positive puppies showed negative PCR tests for B. gibsoni, indicating successful treatment through breast milk during nursing. In the two remaining positive puppies, therapy was started and parasitemia was successfully eliminated.<\/jats:p>","DOI":"10.3390\/pathogens13070562","type":"journal-article","created":{"date-parts":[[2024,7,3]],"date-time":"2024-07-03T11:35:57Z","timestamp":1720006557000},"page":"562","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Successful Transmammary Treatment of Babesia gibsoni Infection in Newborn Puppies after the Administration of Malarone\u00ae, Azithromycin, and Artesunate to a Lactating Dam"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4243-5892","authenticated-orcid":false,"given":"Martina","family":"Karasov\u00e1","sequence":"first","affiliation":[{"name":"Small Animal Clinic, University of Veterinary Medicine and Pharmacy, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Bla\u0148arov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, St. Jakub\u2019s Hospital with Polyclinic, 08501 Bardejov, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7444-7348","authenticated-orcid":false,"given":"Csilla","family":"T\u00f3thov\u00e1","sequence":"additional","affiliation":[{"name":"Clinic of Ruminants, University of Veterinary Medicine and Pharmacy, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6994-8751","authenticated-orcid":false,"given":"Gabriela","family":"Hr\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Pharmacology, Institute of Parasitology, Slovak Academy of Sciences, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1184-9790","authenticated-orcid":false,"given":"Ter\u00e9zia","family":"Kiskov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Animal Physiology, Faculty of Science, University of Pavol Jozef \u0160af\u00e1rik, 04180 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzana","family":"\u0160ev\u010d\u00edkov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viera","family":"Revajov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Morphological Disciplines, University of Veterinary Medicine and Pharmacy, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6613-4616","authenticated-orcid":false,"given":"Oskar","family":"Nagy","sequence":"additional","affiliation":[{"name":"Clinic of Ruminants, University of Veterinary Medicine and Pharmacy, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6538-3044","authenticated-orcid":false,"given":"Bronislava","family":"V\u00edchov\u00e1","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Ecology of Vectors, Institute of Parasitology, Slovak Academy of Sciences, 04001 Ko\u0161ice, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"125","DOI":"10.5326\/15473317-35-2-125","article-title":"Babesia gibsoni Infections in Dogs from North Carolina","volume":"35","author":"Birkenheuer","year":"1999","journal-title":"J. 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