{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T10:41:32Z","timestamp":1776076892066,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T00:00:00Z","timestamp":1720569600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"\u201cPrograma de Apoyo a Proyectos de Investigaci\u00f3n e Innovaci\u00f3n Tecnol\u00f3gica-Direcci\u00f3n General de Asuntos del Personal Acad\u00e9mico\u201d from UNAM-Mexico","award":["IT201318"],"award-info":[{"award-number":["IT201318"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>We evaluated the specificity of the primers OPF-01, P54, and 1253 to identify A. fumigatus, A. flavus, and A. niger, respectively, with the RAPD-PCR method. Eighty-two isolates belonging to the sections Fumigati, Flavi, and Nigri were used. The isolates were identified by phenotypic (macro- and micromorphology) and genotypic (partial sequences of the BenA gene) methods. The RAPD-PCR method was used to obtain polymorphic patterns with the primers OPF-01, P54, and 1253. The specificity of the polymorphic patterns of the isolates of each species was evaluated through the UPGMA clustering method and logistic regression model. All isolates of the genus Aspergillus were identified at the section level by macro- and micromorphology showing the typical morphology of the sections Fumigati, Flavi, and Nigri, and the species were identified by the construction of the phylogeny of the partial sequence of the BenA gene. The patterns\u2019 polymorphic strains obtained with the primers OPF-01, P54, and 1253 for the isolates of A. fumigatus, A. flavus, and A niger, respectively, showed the same polymorphic pattern as the reference strains for each species. To verify the specificity of the primers, they were tested with other species from the sections Fumigati, Flavi and Nigri. The results support that the primers OPF-01, P54, and 1253 generate polymorphic patterns by RAPD-PCR species specific to A. fumigatus, A. flavus, and A. niger, respectively.<\/jats:p>","DOI":"10.3390\/pathogens13070574","type":"journal-article","created":{"date-parts":[[2024,7,10]],"date-time":"2024-07-10T09:23:08Z","timestamp":1720603388000},"page":"574","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Evaluation of Primers OPF-01, P54, and 1253 to Identify A. fumigatus, A. flavus, and A. niger from Polymorphic Patterns Obtained by RAPD-PCR"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1277-7170","authenticated-orcid":false,"given":"Carlos Alberto","family":"Castro-Fuentes","sequence":"first","affiliation":[{"name":"Posgrado en Ciencias Biol\u00f3gicas, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 04510, Mexico"},{"name":"Unidad de Investigaci\u00f3n, Hospital Regional de Alta Especialidad de Ixtapaluca, IMSS-Bienestar. Calle Gustavo E. Campa 54, Col. Guadalupe Inn, Alcald\u00eda \u00c1lvaro Obreg\u00f3n, Mexico City 01020, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3160-8698","authenticated-orcid":false,"given":"Mar\u00eda Guadalupe","family":"Fr\u00edas-De-Le\u00f3n","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n Biom\u00e9dica, Hospital Regional de Alta Especialidad de Ixtapaluca, IMSS-Bienestar. Calle Gustavo E. Campa 54, Col. Guadalupe Inn, Alcald\u00eda \u00c1lvaro Obreg\u00f3n, Mexico City 01020, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda del Carmen","family":"Gonz\u00e1lez-Villase\u00f1or","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Mexico City 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6315-7860","authenticated-orcid":false,"given":"Esperanza","family":"Duarte-Escalante","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 3000, Ciudad Universitaria, Coyoac\u00e1n, Mexico City 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Omar Esteban","family":"Valencia-Ledezma","sequence":"additional","affiliation":[{"name":"Unidad de Investigaci\u00f3n, Hospital Regional de Alta Especialidad de Ixtapaluca, IMSS-Bienestar. Calle Gustavo E. Campa 54, Col. Guadalupe Inn, Alcald\u00eda \u00c1lvaro Obreg\u00f3n, Mexico City 01020, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Areli","family":"Mart\u00ednez-Gamboa","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, Vasco de Quiroga 15, Belisario Dom\u00ednguez Secc. 16, Tlalpan, Mexico City 14080, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Meraz-R\u00edos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 3000, Ciudad Universitaria, Coyoac\u00e1n, Mexico City 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3866-9995","authenticated-orcid":false,"given":"Mar\u00eda del Roc\u00edo","family":"Reyes-Montes","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Medicina, Universidad Nacional Aut\u00f3noma de M\u00e9xico, Avenida Universidad 3000, Ciudad Universitaria, Coyoac\u00e1n, Mexico City 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e24","DOI":"10.1093\/cid\/cir771","article-title":"Outbreak of invasive aspergillosis after major heart surgery caused by spores in the air of the intensive care unit","volume":"54","author":"Guinea","year":"2012","journal-title":"Clin. 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