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This study investigates possible correlations between selected factors, altitude, soil pH, and a factor called \u2018amount\u2019 (number of ticks examined in pooled samples) on the occurrence of I. ricinus ticks positive for selected tick-borne microorganisms. Questing I. ricinus ticks were collected in 2016 and 2017 across various altitudes, at two mountain ranges in central Slovakia. Tick pools were screened for the presence of Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato (Bbsl), Babesia\/Theileria spp., Rickettsia spp., and tick-borne encephalitis virus (TBEV) using molecular methods. Regression analysis was employed to evaluate relationships between selected factors and the occurrence of vector-borne microorganisms. This study revealed a statistically significant influence of altitude on the occurrence of A. phagocytophilum; increasing altitude of the sampling site was associated with increased probability of pathogen occurrence. For Babesia\/Theileria spp., neither altitude nor soil pH significantly affected pathogen occurrence. The occurrence of Bbsl was notably impacted by both altitude and soil pH; higher altitudes were associated with a decreased probability of pathogen presence, whereas higher soil pH increased the likelihood of pathogen occurrence. The presence of Rickettsia in a pooled sample was not affected by altitude and soil pH, but the \u2018amount\u2019 factor was a significant predictor, increasing the probability of pathogen detection. Neither altitude nor soil pH had a significant impact on TBEV occurrence. The regression models showed moderate goodness-of-fit levels to the data, underscoring their utility in examining the role of altitude and soil pH on pathogen occurrence. However, they explained only a small portion of the overall variance in pathogen occurrence, indicating the presence of other significant factors not covered in this study.<\/jats:p>","DOI":"10.3390\/pathogens13070586","type":"journal-article","created":{"date-parts":[[2024,7,15]],"date-time":"2024-07-15T18:17:32Z","timestamp":1721067452000},"page":"586","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Impact of Altitude on Tick-Borne Pathogens at Two Mountain Ranges in Central Slovakia"],"prefix":"10.3390","volume":"13","author":[{"given":"Dana","family":"Zubrikov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Bla\u0148arov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriela","family":"Hrk\u013eov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Catholic University in Ru\u017eomberok, Hrabovsk\u00e1 Cesta 1A, 034 01 Ru\u017eomberok, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaroslav","family":"Syrota","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"},{"name":"I. I. Schmalhausen Institute of Zoology NAS of Ukraine, Bogdan Khmelnytsky Street, 15, 01054 Kyiv, Ukraine"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0658-8015","authenticated-orcid":false,"given":"Jozef","family":"Macko","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Catholic University in Ru\u017eomberok, Hrabovsk\u00e1 Cesta 1A, 034 01 Ru\u017eomberok, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6133-902X","authenticated-orcid":false,"given":"Dana","family":"Blah\u00fatov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Biology and Ecology, Catholic University in Ru\u017eomberok, Hrabovsk\u00e1 Cesta 1A, 034 01 Ru\u017eomberok, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Veronika","family":"Bla\u017eekov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"},{"name":"Department of Epizootiology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Ko\u0161ice, Komensk\u00e9ho 73, 041 81 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9917-2421","authenticated-orcid":false,"given":"Michal","family":"Stanko","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"},{"name":"Institute of Zoology, Slovak Academy of Sciences, D\u00fabravsk\u00e1 Cesta 9, 845 06 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Klaudia","family":"\u0160virlochov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6538-3044","authenticated-orcid":false,"given":"Bronislava","family":"V\u00edchov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, Slovak Academy of Sciences, Hlinkova 3, 040 01 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1079\/9780851996325.0149","article-title":"Ecology of Borrelia burgdorferi Sensu Lato in Europe","volume":"6","author":"Gern","year":"2002","journal-title":"Lyme Borreliosis Biol. 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