{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:18:02Z","timestamp":1760145482545,"version":"build-2065373602"},"reference-count":64,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003141","name":"CONAHCYT","doi-asserted-by":"publisher","award":["1002802","1014987","287598"],"award-info":[{"award-number":["1002802","1014987","287598"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"CONAHCYT-SEP","doi-asserted-by":"publisher","award":["1002802","1014987","287598"],"award-info":[{"award-number":["1002802","1014987","287598"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>This study assessed the immunoprotective effect in lambs of a native excreted\/secreted 15-kDa protein and two synthesised S28 peptides derived from the infective transitory larvae (xL3) and adult stages (AS) of Haemonchus contortus. Twenty-two Pelibuey lambs were divided into negative and positive control groups, as well as immunised lamb groups, with 100 \u00b5g of the 15-kDa native protein (15kDaNP) and S28 peptides (S28P). The eggs per gram (EPG) and haematocrit were measured, and AS were counted and morphologically measured. To assess the immunoprotection in lambs, indirect enzyme-linked immunosorbent assays and relative expression analyses of immune cytokines were performed using serum and abomasal samples. Our results showed a 72.28% reduction in adult worms (AW) in the 15kDaNP-immunised group, achieving a high clinical response with 41% haematocrit and low EPG values (436 \u00b1 661). Conversely, the S28P group achieved the highest IgG levels (2.125 \u00b1 0.880 OD), with AW exhibiting the greatest body length (p &gt; 0.05) and upregulation of the IL5 and FC\u03b5R1A genes associated with nematode control. The 15kDaNP group showed increased expression of genes related to nematode control and anti-inflammatory responses, including IL4, IL5, IL6, and IL13 (p &lt; 0.05). The S28P and 15kDaNP should be explored as potential vaccines against sheep haemonchosis.<\/jats:p>","DOI":"10.3390\/pathogens13070604","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T17:36:04Z","timestamp":1721669764000},"page":"604","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Excreted\/Secreted 15-kDa Proteins and Serine Protease Peptides from Haemonchus contortus Act as Immune-Response Enhancers in Lambs"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2743-5672","authenticated-orcid":false,"given":"Ren\u00e9","family":"Camas-Pereyra","sequence":"first","affiliation":[{"name":"Centro Nacional de Investigaci\u00f3n Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla 8534, Jiutepec 62574, Mexico"},{"name":"Facultad de Medicina Veterinaria y Zootecnia, Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3512-6012","authenticated-orcid":false,"given":"G\u00e9nesis Andrea","family":"Bautista-Garc\u00eda","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7096-0002","authenticated-orcid":false,"given":"Gustavo","family":"P\u00e9rez-Anz\u00farez","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4019-9235","authenticated-orcid":false,"given":"Zaira Carolina","family":"Duran-Cortes","sequence":"additional","affiliation":[{"name":"Ingenier\u00eda en Biotecnolog\u00eda, Universidad Polit\u00e9cnica del Estado de Morelos, Boulevard Cuauhnauhuac #566, Jiutepec 62550, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1979-3755","authenticated-orcid":false,"given":"David Emanuel","family":"Reyes-Guerrero","sequence":"additional","affiliation":[{"name":"Centro Nacional de Investigaci\u00f3n Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla 8534, Jiutepec 62574, Mexico"},{"name":"Facultad de Medicina Veterinaria y Zootecnia, Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9634-9615","authenticated-orcid":false,"given":"Jocelyn","family":"Maza-Lopez","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria y Zootecnia, Ciudad Universitaria, Ciudad de M\u00e9xico 04510, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5499-7449","authenticated-orcid":false,"given":"Agust\u00edn","family":"Olmedo-Ju\u00e1rez","sequence":"additional","affiliation":[{"name":"Centro Nacional de Investigaci\u00f3n Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla 8534, Jiutepec 62574, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8688-4411","authenticated-orcid":false,"given":"Mar\u00eda Eugenia","family":"L\u00f3pez-Arellano","sequence":"additional","affiliation":[{"name":"Centro Nacional de Investigaci\u00f3n Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agr\u00edcolas y Pecuarias, Carr. Fed. Cuernavaca-Cuautla 8534, Jiutepec 62574, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,22]]},"reference":[{"doi-asserted-by":"crossref","unstructured":"Arsenopoulos, K.V., Fthenakis, G.C., Katsarou, E.I., and Papadopoulos, E. (2021). Haemonchosis: A Challenging Parasitic Infection of Sheep and Goats. Animals, 11.","key":"ref_1","DOI":"10.3390\/ani11020363"},{"doi-asserted-by":"crossref","unstructured":"Flay, K.J., Hill, F.I., and Muguiro, D.H. (2022). 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