{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T22:28:42Z","timestamp":1767652122814,"version":"build-2065373602"},"reference-count":59,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T00:00:00Z","timestamp":1722038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consorcio Centro de Investigaci\u00f3n Biom\u00e9dica en Red M.P. (CIBERINFEC) de Enfermedades Infecciosas","award":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"],"award-info":[{"award-number":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"]}]},{"name":"Instituto de Salud Carlos III, 28006 Madrid, Spain","award":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"],"award-info":[{"award-number":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"]}]},{"name":"Fundaci\u00f3n Cajacanarias y Fundaci\u00f3n Bancaria la Caixa","award":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"],"award-info":[{"award-number":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"]}]},{"name":"Agencia Canaria de Investigaci\u00f3n, Innovaci\u00f3n y Sociedad de la Informaci\u00f3n","award":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"],"award-info":[{"award-number":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"]}]},{"name":"Fondo Social Europeo (FSE), RLRE and IS, Cabildo Insular de Tenerife 2023\u20132028","award":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"],"award-info":[{"award-number":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"]}]},{"name":"Ministerio de Sanidad, Spain","award":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"],"award-info":[{"award-number":["CB21\/13\/00100","2022CLISA26","2022CLISA22","TESIS2021010070","CC20230222","CABILDO.23"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The Tajogaite Volcano erupted on the western slope of the Cumbre Vieja mountain range on La Palma Island in the Canary Islands, Spain, in 2021. As one of the multiple consequences of this eruption, a layer of tephra was deposited, to a variable extent, over a large part of the island. Tephra deposits affect all aspects of vegetation recovery, the water cycle, and the long-term availability of volcanic nutrients. Protozoa, including free-living amoeba (FLA), are known to be among the first microorganisms capable of colonizing harsh environments. In the present study, the presence of FLA has been evaluated in the Tajogaite Volcano deposits. Samples of the tephra were collected and incubated at 26 \u00b0C on 2% non-nutrient agar plates with a layer of heat-killed E. coli. Morphological features, as well as the DF3 region sequence of the 18S rDNA, confirmed the presence of a T4 genotype strain of Acanthamoeba. Thermotolerance and osmotolerance assays were used to evaluate the strain\u2019s pathogenic potential. This strain was considered thermotolerant but poorly osmotolerant. To the best of our knowledge, this is the first report of Acanthamoeba being isolated from a recently erupted volcano.<\/jats:p>","DOI":"10.3390\/pathogens13080626","type":"journal-article","created":{"date-parts":[[2024,7,29]],"date-time":"2024-07-29T12:27:43Z","timestamp":1722256063000},"page":"626","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["First Report of Acanthamoeba Genotype T4 from the Newly Formed Tajogaite Volcano Tephra (La Palma, Canary Islands)"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7719-9164","authenticated-orcid":false,"given":"Patricia","family":"P\u00e9rez-P\u00e9rez","sequence":"first","affiliation":[{"name":"Instituto Universitario de Enfermedades Tropicales y Salud P\u00fablica de Canarias (IUETSPC), Universidad de La Laguna (ULL), 38200 San Crist\u00f3bal de La Laguna, Spain"},{"name":"Departamento de Obstetricia y Ginecolog\u00eda, Pediatr\u00eda, Medicina Preventiva y Salud P\u00fablica, Toxicolog\u00eda, Medicina Legal y Forense y Parasitolog\u00eda, Universidad de La Laguna (ULL), 38200 San Crist\u00f3bal de La Laguna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Reyes-Batlle","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Enfermedades Tropicales y Salud P\u00fablica de Canarias (IUETSPC), Universidad de La Laguna (ULL), 38200 San Crist\u00f3bal de La Laguna, Spain"},{"name":"Departamento de Obstetricia y Ginecolog\u00eda, Pediatr\u00eda, Medicina Preventiva y Salud P\u00fablica, Toxicolog\u00eda, Medicina Legal y Forense y Parasitolog\u00eda, Universidad de La Laguna (ULL), 38200 San Crist\u00f3bal de La Laguna, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3858-836X","authenticated-orcid":false,"given":"Rub\u00e9n L.","family":"Rodr\u00edguez-Exp\u00f3sito","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Enfermedades Tropicales y Salud P\u00fablica de Canarias (IUETSPC), Universidad de La Laguna (ULL), 38200 San Crist\u00f3bal de La Laguna, Spain"},{"name":"Departamento de Obstetricia y Ginecolog\u00eda, Pediatr\u00eda, Medicina Preventiva y Salud P\u00fablica, Toxicolog\u00eda, Medicina Legal y Forense y Parasitolog\u00eda, Universidad de La Laguna (ULL), 38200 San Crist\u00f3bal de La Laguna, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos III, 28029 Madrid, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adolfo","family":"Perdomo-Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Animal, Edafolog\u00eda y Geolog\u00eda, Universidad de La Laguna, 38200 San Crist\u00f3bal de La Laguna, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ines","family":"Sifaoui","sequence":"additional","affiliation":[{"name":"Instituto Universitario de Enfermedades Tropicales y Salud P\u00fablica de Canarias (IUETSPC), Universidad de La Laguna (ULL), 38200 San Crist\u00f3bal de La Laguna, Spain"},{"name":"Departamento de Obstetricia y Ginecolog\u00eda, Pediatr\u00eda, Medicina Preventiva y Salud P\u00fablica, Toxicolog\u00eda, Medicina Legal y Forense y Parasitolog\u00eda, Universidad de La Laguna (ULL), 38200 San Crist\u00f3bal de La Laguna, Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Infecciosas (CIBERINFEC), Instituto de Salud Carlos 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Canary Island Seamount Province\u2014New ages of old seamounts","volume":"3","year":"2013","journal-title":"Sci. Rep."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1016\/0377-0273(94)90053-1","article-title":"The Canary Islands: An example of structural control on the growth of large oceanic-island volcanoes","volume":"60","author":"Carracedo","year":"1994","journal-title":"J. Volcanol. Geoth. Res."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1016\/j.ympev.2003.11.018","article-title":"Molecular phylogenetic evidence for the geographic origin and classification of Canary Island Lotus (Fabaceae: Loteae)","volume":"32","author":"Allan","year":"2004","journal-title":"Mol. Phylogenet Evol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"184","DOI":"10.1016\/j.envsci.2018.07.017","article-title":"An institutional analysis to address climate change adaptation in Tenerife (Canary Islands)","volume":"89","author":"Hernandez","year":"2018","journal-title":"Environ. Sci. Policy"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"6336","DOI":"10.1038\/s41598-023-33387-6","article-title":"The ephemeral fumarolic mineralization of the 2021 Tajogaite volcanic eruption (La Palma, Canary Islands, Spain)","volume":"13","author":"Campeny","year":"2023","journal-title":"Sci. Rep."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"134321","DOI":"10.1016\/j.scitotenv.2019.134321","article-title":"Impact of wildfires on subsurface volcanic environments: New insights into speleothem chemistry","volume":"698","author":"Miller","year":"2020","journal-title":"Sci. Total Environ."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"114486","DOI":"10.1016\/j.envres.2022.114486","article-title":"A longitudinal and multidesign epidemiological study to analyze the effect of the volcanic eruption of Tajogaite volcano (La Palma, Canary Islands). The ASHES study protocol","volume":"216","author":"Acosta","year":"2023","journal-title":"Environ. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3174","DOI":"10.1038\/s41467-022-30905-4","article-title":"Eruption of ultralow-viscosity basanite magma at Cumbre Vieja, La Palma, Canary Islands","volume":"13","author":"Castro","year":"2022","journal-title":"Nat. Commun."},{"key":"ref_9","unstructured":"Cabildo La Palma (2022, April 10). Available online: https:\/\/riesgovolcanico-lapalma.hub.arcgis.com."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.mimet.2007.04.005","article-title":"Exploring microbial diversity in volcanic environments: A review of methods in DNA extraction","volume":"70","author":"Herrera","year":"2007","journal-title":"J. Microbiol. Methods"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s12639-017-0950-2","article-title":"Free-living amoebae in the water resources of Iran: A systematic review","volume":"41","author":"Saburi","year":"2017","journal-title":"J. Parasit. Dis."},{"key":"ref_12","first-page":"143","article-title":"Rosculus ithacus Hawes, 1963, Amoebida, Flabellulidea and the amphizoic tendency in amoebae","volume":"13","author":"Page","year":"1974","journal-title":"Acta Protozool."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1574-695X.2007.00232.x","article-title":"Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp., Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea","volume":"50","author":"Visvesvara","year":"2007","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1999","DOI":"10.1007\/s00436-019-06312-y","article-title":"Vermamoeba vermiformis as etiological agent of a painful ulcer close to the eye","volume":"118","author":"Scheid","year":"2019","journal-title":"Parasitol. Res."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Siddiqui, R., Makhlouf, Z., and Khan, N.A. (2021). The increasing importance of Vermamoeba vermiformis. J. Eukaryot. Microbiol., 68.","DOI":"10.1111\/jeu.12857"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1097\/ICO.0000000000001226","article-title":"Free-Living Amoebae Keratitis","volume":"36","author":"Pinna","year":"2017","journal-title":"Cornea"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1111\/j.1550-7408.1998.tb05068.x","article-title":"The evolutionary history of the genus Acanthamoeba and the identification of eight new 18S rRNA gene sequence types","volume":"45","author":"Stothard","year":"1998","journal-title":"J. Eukaryot. Microbiol."},{"key":"ref_18","first-page":"233","article-title":"Phylogenetic evidence for a new genotype of Acanthamoeba (Amoebozoa, Acanthamoebida) Parasitol","volume":"107","author":"Corsaro","year":"2010","journal-title":"Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"498","DOI":"10.1111\/j.1550-7408.1996.tb04510.x","article-title":"Subgenus systematics of Acanthamoeba: Four nuclear 18S rDNA sequence types","volume":"43","author":"Gast","year":"1996","journal-title":"J. Eukaryot. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"17290","DOI":"10.1038\/s41598-021-96690-0","article-title":"Analysis of Acanthamoeba genotypes from public freshwater sources in Thailand reveals a new genotype, T23 Acanthamoeba bangkokensis sp. nov","volume":"11","author":"Putaporntip","year":"2021","journal-title":"Sci Rep."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1","DOI":"10.4067\/S0301-732X2016000100001","article-title":"Interaction between zoonotic bacteria and free-living amoebas: A new angle of an epidemiological polyhedron of public health importance?","volume":"48","author":"Mella","year":"2016","journal-title":"Arch. Med. Vet."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1111\/j.1472-765X.2008.02457.x","article-title":"Hartmanella vermiformis can be permissive for Pseudomonas aeruginosa","volume":"47","author":"Cateau","year":"2008","journal-title":"Lett. Appl. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"3033","DOI":"10.1007\/s00436-022-07631-3","article-title":"Prevalence of free-living amoebae in swimming pools and recreational waters, a systematic review and meta-analysis","volume":"121","author":"Anvari","year":"2022","journal-title":"Parasitol. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2953","DOI":"10.1007\/s00436-016-5048-8","article-title":"Acanthamoeba genotypes T2, T4, and T11 in soil sources from El Hierro island, Canary Islands, Spain","volume":"115","author":"Wagner","year":"2016","journal-title":"Parasitol. Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1007\/s00436-014-3778-z","article-title":"Isolation and characterization of Acanthamoeba strains from soil samples in Gran Canaria, Canary Islands, Spain","volume":"113","author":"Todd","year":"2014","journal-title":"Parasitol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"394","DOI":"10.2307\/3224707","article-title":"Mitosis and pseudopod formation in Vexillifera bacillipedes n. sp.; A majorellid amoeba","volume":"88","author":"Page","year":"1969","journal-title":"Trans. Am. Microsc. Soc."},{"key":"ref_27","unstructured":"Page, F.C. (1988). A New Key to Fresh Water and Soil Gymnamoebae, Freshwater Biological Association, Ambleside."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1007\/s002840010288","article-title":"Acanthamoeba can be differentiated by the polymerase chain reaction and simple plating assays","volume":"43","author":"Khan","year":"2001","journal-title":"Curr. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1111\/jeu.12197","article-title":"Isolation and genotyping of Acanthamoeba strains from soil sources from Jamaica, West Indies","volume":"62","author":"Todd","year":"2015","journal-title":"J. Eukaryot. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1903","DOI":"10.1128\/JCM.39.5.1903-1911.2001","article-title":"Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge","volume":"39","author":"Schroeder","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"3022","DOI":"10.1093\/molbev\/msab120","article-title":"MEGA 11, Molecular Evolutionary Genetics Analysis Version 11","volume":"38","author":"Tamura","year":"2021","journal-title":"Mol. Biol. Evol."},{"key":"ref_32","first-page":"512","article-title":"Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees","volume":"10","author":"Tamura","year":"1993","journal-title":"Mol. Biol. Evol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1111\/j.1550-7408.1967.tb02066.x","article-title":"Re-definition of the genus Acanthamoeba with descriptions of three species","volume":"14","author":"Page","year":"1967","journal-title":"J. Protozool."},{"key":"ref_34","first-page":"557","article-title":"Morphologie de la paroi kystique et taxonomie du genre Acanthamoeba (Protozoa, Amoebida)","volume":"13","author":"Pussard","year":"1977","journal-title":"Protistologica"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Wang, Y., Jiang, L., Zhao, Y., Ju, X., Wang, L., Jin, L., Fine, R.D., and Li, M. (2023). Biological characteristics and pathogenicity of Acanthamoeba. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1147077"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/S0013-9351(87)80016-6","article-title":"Amoebae isolated from the atmosphere of Mexico City and environs","volume":"42","author":"Rivera","year":"1987","journal-title":"Environ. Res."},{"key":"ref_37","first-page":"285","article-title":"Acanthamoeba spp. from water and soil sources in Iran: A systematic review and meta-analysis","volume":"64","author":"Kialashaki","year":"2018","journal-title":"Ann. Parasitol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3151","DOI":"10.1007\/s00436-014-3976-8","article-title":"Acanthamoeba everywhere: High diversity of Acanthamoeba in soils","volume":"113","author":"Geisen","year":"2014","journal-title":"Parasitol. Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1007\/s00436-005-1301-2","article-title":"Isolation and identification of pathogenic Acanthamoeba strains in Tenerife, Canary Islands, Spain from water sources","volume":"95","author":"Foronda","year":"2005","journal-title":"Parasitol. Res."},{"key":"ref_40","first-page":"233","article-title":"Evaluation of Acanthamoeba isolates from environmental sources in Tenerife, Canary Islands, Spain","volume":"12","author":"Tejedor","year":"2005","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/BF00369389","article-title":"1980 tephra from Mount St. Helens: Spatial and temporal variation beneath forest canopies","volume":"12","author":"Zobel","year":"1991","journal-title":"Biol. Fertil. Soils"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1890\/0012-9615(1997)067[0317:ADOROU]2.0.CO;2","article-title":"A decade of recovery of understory vegetation buried by volcanic tephra from Mount St. Helens","volume":"67","author":"Zobel","year":"1997","journal-title":"Ecol. Monogr."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"109949","DOI":"10.1016\/j.dib.2023.109949","article-title":"Volcanic tephra deposition dataset based on interpolated field measurements following the 2021 Tajogaite Eruption on La Palma, Canary Islands, Spain","volume":"52","author":"Shatto","year":"2023","journal-title":"Data Brief."},{"key":"ref_44","unstructured":"Keller, S.A.C. (1986). Chemical composition of the May 18, 1980, Mount St. Helens tephra and the underlying soil: Changes from July 1980 to May 1981. Helens: Five Years Later, Mount St. Eastern Washington University, Orvallis, USA Press."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1007\/s00445-004-0370-x","article-title":"Surface area, porosity and water adsorption properties of fine volcanic ash particles","volume":"67","author":"Delmelle","year":"2005","journal-title":"Bull. Volcanol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.ejop.2016.05.003","article-title":"Cinderella\u2019s helping pigeons of the microbial world: The potential of testate amoebae for identifying cryptotephra","volume":"55 Pt. B","author":"Delaine","year":"2016","journal-title":"Eur. J. Protistol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1007\/s00248-014-0432-3","article-title":"Pioneer Microbial Communities of the Fimmv\u00f6r\u00f0uh\u00e1ls Lava Flow, Eyjafjallaj\u00f6kull, Iceland","volume":"68","author":"Kelly","year":"2014","journal-title":"Microb. Ecol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1007\/s00248-017-1129-1","article-title":"Microbial Communities of High-Elevation Fumaroles, Penitentes, and Dry Tephra \u201cSoils\u201d of the Puna de Atacama Volcanic Zone","volume":"76","author":"Solon","year":"2018","journal-title":"Microb. Ecol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"775","DOI":"10.1007\/s00248-016-0873-y","article-title":"Development of Soil Bacterial Communities in Volcanic Ash Microcosms in a Range of Climates","volume":"73","author":"Kerfahi","year":"2017","journal-title":"Microb. Ecol."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Rayamajhee, B., Willcox, M., Henriquez, F.L., Vijay, A.K., Petsoglou, C., Shrestha, G.S., Peguda, H.K., and Carnt, N. (2024). The role of naturally acquired intracellular Pseudomonas aeruginosa in the development of Acanthamoeba keratitis in an animal model. PLoS Neglected Trop. Dis., 18.","DOI":"10.1371\/journal.pntd.0011878"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e00025-22","DOI":"10.1128\/spectrum.00025-22","article-title":"A Comparative Genomic Approach to Determine the Virulence Factors and Horizontal Gene Transfer Events of Clinical Acanthamoeba Isolates","volume":"10","author":"Gu","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_52","first-page":"20","article-title":"An update on Acanthamoeba keratitis: Diagnosis, pathogenesis and treatment","volume":"22","author":"Khan","year":"2015","journal-title":"Parasite"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.ejop.2012.11.004","article-title":"A systematic analysis of Acanthamoeba genotype frequency correlated with source and pathogenicity: T4 is confirmed as a pathogen-rich genotype","volume":"49","author":"Maciver","year":"2013","journal-title":"Eur. J. Protistol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1735","DOI":"10.2166\/wh.2023.360","article-title":"First report of Acanthamoebae spp. isolation from a volcanic mud spring in the Philippines","volume":"21","author":"Celis","year":"2023","journal-title":"J. Water Health"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1016\/j.ijpara.2004.06.004","article-title":"Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and ani- mals","volume":"34","author":"Schuster","year":"2004","journal-title":"Int. J. Parasitol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"81","DOI":"10.2478\/s11756-009-0217-1","article-title":"Morphological, physiological, molecular and phylogenetic characterization of new environmental isolates of Acanthamoeba spp. from the region of Bratislava, Slovakia","volume":"65","author":"Nagy","year":"2010","journal-title":"Biologia"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"97","DOI":"10.14411\/fp.2003.017","article-title":"Genotypic, phenotypic, biochemical, physiological and pathogenicity-based categorization of Acanthamoeba strains","volume":"50","author":"Khan","year":"2003","journal-title":"Folia Parasitol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.exppara.2009.12.008","article-title":"Screening of Swiss hot spring resorts for potentially pathogenic free-living amoebae","volume":"126","author":"Gianinazzi","year":"2010","journal-title":"Exp. Parasitol."},{"key":"ref_59","unstructured":"Fern\u00e1ndez Rodrigo, M.T. (2014). Caracterizaci\u00f3n Molecular de Amebas de Vida Libre e Identificaci\u00f3n de Otros Par\u00e1sitos en Aguas de red de la Provincia de Zaragoza; Asociaci\u00f3n con Otros Microorganismos y Riesgos para la Salud P\u00fablica. [Ph.D. Thesis, Universidad de Zaragoza]."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/8\/626\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:24:50Z","timestamp":1760109890000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/8\/626"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,7,27]]},"references-count":59,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["pathogens13080626"],"URL":"https:\/\/doi.org\/10.3390\/pathogens13080626","relation":{},"ISSN":["2076-0817"],"issn-type":[{"type":"electronic","value":"2076-0817"}],"subject":[],"published":{"date-parts":[[2024,7,27]]}}}