{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T19:08:29Z","timestamp":1768676909849,"version":"3.49.0"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T00:00:00Z","timestamp":1722384000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"China Agriculture Research System","award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}]},{"name":"China Agriculture Research System","award":["2023TD49"],"award-info":[{"award-number":["2023TD49"]}]},{"name":"China Agriculture Research System","award":["2024KJ150"],"award-info":[{"award-number":["2024KJ150"]}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"publisher","award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"publisher","award":["2023TD49"],"award-info":[{"award-number":["2023TD49"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, CAFS","doi-asserted-by":"publisher","award":["2024KJ150"],"award-info":[{"award-number":["2024KJ150"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams","award":["CARS-46"],"award-info":[{"award-number":["CARS-46"]}]},{"name":"Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams","award":["2023TD49"],"award-info":[{"award-number":["2023TD49"]}]},{"name":"Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams","award":["2024KJ150"],"award-info":[{"award-number":["2024KJ150"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Outbreaks of bacterial diseases occur in farmed Chinese longsnout catfish (Leiocassis longirostris). Due to limited information on aquatic Klebsiella variicola-infected animals, this study aimed to identify strain LL2208 isolated from diseased L. longirostris, determine its biological features, and evaluate its risk to public health. Strain LL2208 was tested for molecular identification, challenge, string, biofilm formation, and antimicrobial susceptibility. Furthermore, the whole genome of the strain was sequenced and analyzed. Based on molecular identification, strain LL2208 was identified as K. variicola. Artificial infection showed that this strain was moderately virulent to L. longirostris with an LD50 = 7.92 \u00d7 107 CFU\/mL. Antibiotic sensitivity tests showed that this strain was resistant to penicillins, macrolides, aminoglycosides, amphenicols, glycopeptides, and lincosamide, indicating multidrug resistance. Strain LL2208 has a genome size of 5,557,050 bp, with a GC content of 57.38%, harboring 30 antimicrobial resistance genes and numerous virulence-related genes. Its molecular type was ST595-KL16-O5. Collinearity analysis showed that strain LL2208 was highly similar to the human-derived K. variicola strain. In conclusion, the multidrug-resistant and virulent K. variicola strain LL2208 was isolated from fish and may have originated from humans. These results provide a foundation for further studies on the transmission of K. variicola between humans and aquatic animals.<\/jats:p>","DOI":"10.3390\/pathogens13080647","type":"journal-article","created":{"date-parts":[[2024,7,31]],"date-time":"2024-07-31T17:16:49Z","timestamp":1722446209000},"page":"647","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Hypermucoviscous Multidrug-Resistant Klebsiella variicola Strain LL2208 Isolated from Chinese Longsnout Catfish (Leiocassis longirostris): Highly Similar to Human K. variicola Strains"],"prefix":"10.3390","volume":"13","author":[{"given":"Qingyong","family":"Li","sequence":"first","affiliation":[{"name":"Fisheries Research and Extension Center of Huizhou, Huizhou 516055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin","family":"Yu","sequence":"additional","affiliation":[{"name":"Fisheries Research and Extension Center of Huizhou, Huizhou 516055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Ye","sequence":"additional","affiliation":[{"name":"Fisheries Research and Extension Center of Huizhou, Huizhou 516055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tongyu","family":"Hou","sequence":"additional","affiliation":[{"name":"Fisheries Research and Extension Center of Huizhou, Huizhou 516055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Fisheries Research and Extension Center of Huizhou, Huizhou 516055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guiming","family":"Liu","sequence":"additional","affiliation":[{"name":"Fisheries Research and Extension Center of Huizhou, Huizhou 516055, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5574-008X","authenticated-orcid":false,"given":"Defeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"},{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Immunology and Sustainable Aquaculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"104768","DOI":"10.1016\/j.meegid.2021.104768","article-title":"Comprehensive genomic analysis and characterization of a new ST 174 type Klebsiella variicola strain isolated from chicken embryos","volume":"90","author":"Yin","year":"2021","journal-title":"Infect. 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