{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T06:14:00Z","timestamp":1780380840947,"version":"3.54.1"},"reference-count":35,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T00:00:00Z","timestamp":1722556800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of Food and Agriculture","award":["719 MIN-62-137"],"award-info":[{"award-number":["719 MIN-62-137"]}]},{"name":"National Institute of Food and Agriculture","award":["23-079 SHIC"],"award-info":[{"award-number":["23-079 SHIC"]}]},{"name":"Swine Health Information Center","award":["719 MIN-62-137"],"award-info":[{"award-number":["719 MIN-62-137"]}]},{"name":"Swine Health Information Center","award":["23-079 SHIC"],"award-info":[{"award-number":["23-079 SHIC"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Specimens collected from dead pigs are a welfare-friendly and cost-effective active surveillance. This study aimed to evaluate the accuracy of different postmortem specimens from dead piglets for disease detection, using PRRSV as an example. Three farrow-to-wean farms undergoing PRRSV elimination were conveniently selected. Samples were collected at approximately 8- and 20-weeks post-outbreak. Postmortem specimens included nasal (NS), oral (OS), and rectal (RS) swabs, tongue-tip fluids (TTF), superficial inguinal lymph nodes (SIL), and intracardiac blood. These were tested individually for PRRSV by RT-PCR. Sensitivity, specificity, negative and positive predictive values, and agreement of postmortem specimens were calculated using intracardiac sera as the gold standard. OS and SIL had the best overall performance, with sensitivities of 94.6\u2013100%, specificities of 83.9\u201385.1%, and negative predictive values of 97.3\u2013100%. TTF had high sensitivity (92.2%) but low specificity (53.9%) and positive predictive value (48.3%). While challenges in meeting sampling targets due to variable pre-weaning mortality were noted, PRRS was detected in all postmortem specimens. OS and NS showed promising results for disease monitoring, though TTF, despite their sensitivity, had lower specificity, making them less suitable for individual infection assessment but useful for assessing environmental contamination.<\/jats:p>","DOI":"10.3390\/pathogens13080649","type":"journal-article","created":{"date-parts":[[2024,8,2]],"date-time":"2024-08-02T09:34:17Z","timestamp":1722591257000},"page":"649","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Postmortem Sampling in Piglet Populations: Unveiling Specimens Accuracy for Porcine Reproductive and Respiratory Syndrome Detection"],"prefix":"10.3390","volume":"13","author":[{"given":"Mariana","family":"Kikuti","sequence":"first","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudio Marcello","family":"Melini","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomei","family":"Yue","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3547-7576","authenticated-orcid":false,"given":"Marie","family":"Culhane","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2749-3496","authenticated-orcid":false,"given":"Cesar A.","family":"Corzo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Population Medicine, University of Minnesota, Saint Paul, MN 55108, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/j.virusres.2010.08.016","article-title":"Control and elimination of porcine reproductive and respiratory syndrome virus","volume":"154","author":"Corzo","year":"2010","journal-title":"Virus Res."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1016\/j.prevetmed.2014.05.010","article-title":"Comparison of time to PRRSv-stability and production losses between two exposure programs to control PRRSv in sow herds","volume":"116","author":"Linhares","year":"2014","journal-title":"Prev. Vet. Med."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"385","DOI":"10.2460\/javma.2005.227.385","article-title":"Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States","volume":"227","author":"Neumann","year":"2005","journal-title":"J. Am. Vet. Med. Assoc."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"72","DOI":"10.54846\/jshap\/754","article-title":"Assessment of the economic impact of porcine reproductive and respiratory syndrome virus on United States pork producers","volume":"21","author":"Holtkamp","year":"2013","journal-title":"J. Swine Health Prod."},{"key":"ref_5","unstructured":"Morrison Swine Health Monitoring Project (2024, July 27). PRRS Prevalence of Sow Herd Status Beginning 1 July 2009. Available online: https:\/\/mshmp.umn.edu\/reports#Charts."},{"key":"ref_6","unstructured":"Morrison Swine Health Monitoring Project (2024, July 27). PRRS Cumulative Incidence Beginning 1 July 2009. Available online: https:\/\/mshmp.umn.edu\/reports#Charts."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"625","DOI":"10.1177\/1040638719852999","article-title":"Effect of litter aggregation and pooling on detection of porcine reproductive and respiratory virus in piglet processing fluids","volume":"31","author":"Vilalta","year":"2019","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"105211","DOI":"10.1016\/j.prevetmed.2020.105211","article-title":"Assessing the litter level agreement of RT-PCR results for porcine reproductive and respiratory syndrome virus in testicles, tails and udder wipes diagnostic samples relative to serum from piglets","volume":"186","author":"Vilalta","year":"2021","journal-title":"Prev. Vet. Med."},{"key":"ref_9","unstructured":"USDA (2024, July 27). Categorizing Animal Pain or Distress in Research Facility Annual Reports, Available online: https:\/\/www.aphis.usda.gov\/sites\/default\/files\/ac-tech-note-categorizing-animal-pain-or-distress.pdf."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"146","DOI":"10.54846\/jshap\/1055","article-title":"Porcine reproductive and respiratory syndrome monitoring in breeding herds using processing fluids","volume":"26","author":"Lopez","year":"2018","journal-title":"J. Swine Health Prod."},{"key":"ref_11","unstructured":"Official Journal of the European Union (2024, July 27). COUNCIL DIRECTIVE 2008\/120\/EC of 18 December 2008 Laying down Minimum Standards for the Protection of Pigs. Available online: https:\/\/eur-lex.europa.eu\/legal-content\/EN\/TXT\/PDF\/?uri=CELEX:32008L0120."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Baliellas, J., Novell, E., Enric-Taranc\u00f3n, V., Vilalta, C., and Fraile, L. (2021). Porcine Reproductive and Respiratory Syndrome Surveillance in breeding Herds and Nurseries Using Tongue Tips from Dead Animals. Vet. Sci., 8.","DOI":"10.3390\/vetsci8110259"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Machado, I.F., Magalh\u00e3es, E.S., Poeta Silva, A.P.S., Moraes, D.C.A., Cezar, G., Mil-Homens, M.P., Osemeke, O.H., Paiva, R., Moura, C.A.A., and Gauger, P. (2022). Porcine reproductive and respiratory syndrome virus RNA detection in tongue tips from dead animals. Front. Vet. Sci., 9.","DOI":"10.3389\/fvets.2022.993442"},{"key":"ref_14","first-page":"e06707","article-title":"Assessment of the control measures of the category A diseases of Animal Health Law: Classical Swine Fever","volume":"19","author":"Nielsen","year":"2021","journal-title":"EFSA J."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Goonewardene, K.B., Onyilagha, C., Goolia, M., Le, V.P., Blome, S., and Ambagala, A. (2022). Superficial Inguinal Lymph Nodes for Screening Dead Pigs for African Swine Fever. Viruses, 14.","DOI":"10.3390\/v14010083"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Perez, A.M., Linhares, D.C.L., Arruda, A.G., VanderWaal, K., Machado, G., Vilalta, C., Sanhueza, J.M., Torrison, J., Torremorell, M., and Corzo, C.A. (2019). Individual or Common Good? Voluntary Data Sharing to Inform Disease Surveillance Systems in Food Animals. Front. Vet. Sci., 6.","DOI":"10.3389\/fvets.2019.00194"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"261","DOI":"10.54846\/jshap\/1218","article-title":"Proposed modifications to porcine reproductive and respiratory syndrome virus herd classification","volume":"29","author":"Holtkamp","year":"2021","journal-title":"J Swine Health Prod."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"44","DOI":"10.54846\/jshap\/669","article-title":"Terminology for classifying swine herds by porcine reproductive and respiratory syndrome virus status","volume":"19","author":"Holtkamp","year":"2011","journal-title":"J. Swine Health Prod."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"8700","DOI":"10.1128\/JVI.02551-09","article-title":"Phylogeny-Based Evolutionary, Demographical, and Geographical Dissection of North American Type 2 Porcine Reproductive and Respiratory Syndrome Viruses","volume":"84","author":"Shi","year":"2010","journal-title":"J. Virol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Paploski, I.A.D., Corzo, C., Rovira, A., Murtaugh, M.P., Sanhueza, J.M., Vilalta, C., Schroeder, D.C., and VanderWaal, K. (2019). Temporal Dynamics of Co-circulating Lineages of Porcine Reproductive and Respiratory Syndrome Virus. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02486"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e02916-23","DOI":"10.1128\/spectrum.02916-23","article-title":"Refining PRRSV-2 genetic classification based on global ORF5 sequences and investigation of their geographic distributions and temporal changes","volume":"11","author":"Anderson","year":"2023","journal-title":"Microbiol. Spectr."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Paploski, I.A.D., Pamornchainavakul, N., Makau, D.N., Rovira, A., Corzo, C.A., Schroeder, D.C., Cheeran, M.C.-J., Doeschl-Wilson, A., Kao, R.R., and Lycett, S. (2021). Phylogenetic Structure and Sequential Dominance of Sub-Lineages of PRRSV Type-2 Lineage 1 in the United States. Vaccines, 9.","DOI":"10.3390\/vaccines9060608"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"VanderWaal, K., Pamornchainvakul, N., Kikuti, M., Linhares, D., Trevisan, G., Zhang, J., Anderson, T., Zeller, M., Rossow, S., and Holtkamp, D. (2024). Phylogenetic-based methods for fine-scale classification of PRRSV-2 ORF5 sequences: A comparison of their robustness and reproducibility. bioRxiv.","DOI":"10.1101\/2024.05.13.593920"},{"key":"ref_24","unstructured":"(2023). StataCorp Stata Statistical Software, Release 18 2023, StataCorp."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Kikuti, M., Paploski, I.A.D., Pamornchainavakul, N., Picasso-Risso, C., Schwartz, M., Yeske, P., Leuwerke, B., Bruner, L., Murray, D., and Roggow, B.D. (2021). Emergence of a New Lineage 1C Variant of Porcine Reproductive and Respiratory Syndrome Virus 2 in the United States. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.752938"},{"key":"ref_26","first-page":"170","article-title":"Clinical signs and their association with herd demographics and porcine reproductive and respiratory syndrome (PRRS) control strategies in PRRS PCR-positive swine herds in Ontario","volume":"74","author":"Young","year":"2010","journal-title":"Can. J. Vet. Res. = Rev. Can. Rech. Vet."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Kikuti, M., Vilalta, C., Sanhueza, J., Pamornchainavakul, N., Kevill, J., Yang, M., Paploski, I.A.D., Lenskaia, T., Odogwu, N.M., and Kiehne, R. (2023). Porcine Reproductive and Respiratory Syndrome (PRRSV2) Viral Diversity within a Farrow-to-Wean Farm Cohort Study. Viruses, 15.","DOI":"10.3390\/v15091837"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"347","DOI":"10.1016\/S0378-1135(96)01319-3","article-title":"Strategies to control PRRS: A summary of field and research experiences","volume":"55","author":"Dee","year":"1997","journal-title":"Vet. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Clilverd, H., Mart\u00edn-Valls, G., Li, Y., Mart\u00edn, M., Cortey, M., and Mateu, E. (2023). Infection dynamics, transmission, and evolution after an outbreak of porcine reproductive and respiratory syndrome virus. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1109881"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1186\/s40813-024-00370-0","article-title":"Detection of PRRSV-1 in tongue fluids under experimental and field conditions and comparison of different sampling material for PRRSV sow herd monitoring","volume":"10","author":"Kreutzmann","year":"2024","journal-title":"Porc. Health Manag."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1186\/1743-422X-6-177","article-title":"Age-dependent resistance to Porcine reproductive and respiratory syndrome virus replication in swine","volume":"6","author":"Klinge","year":"2009","journal-title":"Virol. J."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.jcv.2008.12.017","article-title":"PCR inhibition in stool samples in relation to age of infants","volume":"44","author":"Oikarinen","year":"2009","journal-title":"J. Clin. Virol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/S0378-1135(97)00079-5","article-title":"Porcine reproductive and respiratory syndrome virus: Routes of excretion","volume":"57","author":"Wills","year":"1997","journal-title":"Vet. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1177\/104063879400600102","article-title":"Experimental Porcine Reproductive and Respiratory Syndrome Virus Infection in One-, Four-, and 10-Week-Old Pigs","volume":"6","author":"Rossow","year":"1994","journal-title":"J. Vet. Diagn. Investig."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Osemeke, O.H., Cezar, G.A., Paiva, R.C., Moraes, D.C.A., Machado, I.F., Magalhaes, E.S., Poeta Silva, A.P.S., Mil-Homens, M., Peng, L., and Jayaraman, S. (2023). A cross-sectional assessment of PRRSV nucleic acid detection by RT-qPCR in serum, ear-vein blood swabs, nasal swabs, and oral swabs from weaning-age pigs under field conditions. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1200376"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/8\/649\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:28:38Z","timestamp":1760110118000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/8\/649"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,8,2]]},"references-count":35,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2024,8]]}},"alternative-id":["pathogens13080649"],"URL":"https:\/\/doi.org\/10.3390\/pathogens13080649","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,8,2]]}}}