{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:21:39Z","timestamp":1760145699840,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T00:00:00Z","timestamp":1723680000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Program for Scientific Research and Advanced Studies, PROCIENCIA-CONCYTEC","award":["403-2019-FONDECYT"],"award-info":[{"award-number":["403-2019-FONDECYT"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Carrion\u2019s disease, caused by Bartonella bacilliformis, is a neglected tropical disease prevalent in the Andean region of South America. Without antimicrobial treatment, this disease has a mortality rate of up to 88% in infected patients. The most common method for diagnosing B. bacilliformis infection is serological testing. However, the current serological assays are limited in sensitivity and specificity, underscoring the need for the development of novel and more accurate diagnostic tools. Recombinant proteins have emerged as promising candidates to improve the serological diagnosis of Carrion\u2019s disease. So, we focused on evaluating the conditions for producing two previously predicted proteins of B. bacilliformis using the baculovirus\u2013insect cell expression system, mainly the flashBAC ULTRA technology. We assessed various parameters to identify the conditions that yield the highest protein production, including cell lines, temperature, and hours post-infection (hpi). The results showed that the expression conditions for achieving the highest yields of the Prot_689 and Prot_504 proteins were obtained using High Five\u2122 cells at 21 \u00b0C and harvesting at 120 hpi. Subsequently, the seroreactivity of recombinant proteins was evaluated using positive sera from patients diagnosed with Carrion\u2019s disease. These findings offer valuable insights into the production conditions of B. bacilliformis recombinant proteins using the baculovirus system, which could significantly contribute to developing more precise diagnostic tools for Carrion\u2019s disease. Therefore, this research provides implications for improving diagnostics and potentially developing therapeutic strategies.<\/jats:p>","DOI":"10.3390\/pathogens13080690","type":"journal-article","created":{"date-parts":[[2024,8,15]],"date-time":"2024-08-15T03:49:36Z","timestamp":1723693776000},"page":"690","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Baculovirus-Assisted Production of Bartonella bacilliformis Proteins: A Potential Strategy for Improving Serological Diagnosis of Carrion\u2019s Disease"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9373-6655","authenticated-orcid":false,"given":"Lizbeth Sally","family":"Vilca-Machaca","sequence":"first","affiliation":[{"name":"Faculty of Biological Sciences, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru"},{"name":"Laboratory of Vector-Borne and Zoonotic Bacterial Diseases, National Institute of Health, Lima 15072, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0438-9602","authenticated-orcid":false,"given":"Karen Daphne","family":"Calvay-Sanchez","sequence":"additional","affiliation":[{"name":"Laboratory of Vector-Borne and Zoonotic Bacterial Diseases, National Institute of Health, Lima 15072, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6699-7507","authenticated-orcid":false,"given":"Yanina","family":"Zarate-Sulca","sequence":"additional","affiliation":[{"name":"Laboratory of Vector-Borne and Zoonotic Bacterial Diseases, National Institute of Health, Lima 15072, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6547-6999","authenticated-orcid":false,"given":"Victor","family":"Jimenez-Vasquez","sequence":"additional","affiliation":[{"name":"Laboratory of Vector-Borne and Zoonotic Bacterial Diseases, National Institute of Health, Lima 15072, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9309-7021","authenticated-orcid":false,"given":"Pablo","family":"Ramirez","sequence":"additional","affiliation":[{"name":"Faculty of Biological Sciences, Universidad Nacional Mayor de San Marcos, Lima 15081, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7000-7636","authenticated-orcid":false,"given":"Giovanna","family":"Mendoza-Mujica","sequence":"additional","affiliation":[{"name":"Laboratory of Vector-Borne and Zoonotic Bacterial Diseases, National Institute of Health, Lima 15072, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"344","DOI":"10.4269\/ajtmh.1999.61.344","article-title":"An Outbreak of Acute Bartonellosis (Oroya Fever) in the Urubamba Region of Peru, 1998","volume":"61","author":"Beati","year":"1999","journal-title":"Am. 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