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Nonetheless, there has been a scarcity of systematic reports on EP parasites in donkeys in Kyrgyzstan, Central Asia. In this study, piroplasms were screened in 1900 blood samples from imported donkeys from the Osh Oblast (southwestern Kyrgyzstan) by targeting partial 18S ribosomal RNA using the polymerase chain reaction (PCR). Through molecular and phylogenetic analyses, all positive samples were sequenced to identify the species and genotypes. The results indicated the presence of both B. caballi and T. equi, with prevalence rates of 8.4% (160\/1900) and 12.2% (232\/1900), respectively. By amplifying part of the Erythrocyte Merozoite Antigen 1 (EMA-1) and Rhoptry-Associated Protein (RAP-1) genes, B. caballi genotype B and T. equi genotype A were identified. To the best of our knowledge, this is the first report on piroplasm infection among donkeys from Kyrgyzstan.<\/jats:p>","DOI":"10.3390\/pathogens13090713","type":"journal-article","created":{"date-parts":[[2024,8,26]],"date-time":"2024-08-26T03:32:01Z","timestamp":1724643121000},"page":"713","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["First Molecular Evidence of Babesia caballi and Theileria equi in Imported Donkeys from Kyrgyzstan"],"prefix":"10.3390","volume":"13","author":[{"given":"Xuanchen","family":"Wu","sequence":"first","affiliation":[{"name":"NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, School of Medicine, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Xu","sequence":"additional","affiliation":[{"name":"Urumqi Customs, Urumqi 833400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lixin","family":"Su","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, School of Medicine, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ente","family":"Li","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, School of Medicine, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suwen","family":"Wang","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, School of Medicine, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00e1ndor","family":"Hornok","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Zoology, University of Veterinary Medicine, 1078 Budapest, Hungary"},{"name":"HUN-REN-UVMB Climate Change: New Blood-Sucking Parasites and Vector-Borne Pathogens Research Group, 1078 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gang","family":"Liu","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, School of Medicine, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuanzhi","family":"Wang","sequence":"additional","affiliation":[{"name":"NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, School of Medicine, Shihezi University, Shihezi 832002, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,8,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"679","DOI":"10.1016\/j.ijpara.2018.03.010","article-title":"Discovery of a Novel Species, Theileria haneyi n. sp., Infective to Equids, Highlights Exceptional Genomic Diversity within the Genus Theileria: Implications for Apicomplexan Parasite Surveillance","volume":"48","author":"Knowles","year":"2018","journal-title":"Int. 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