{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T11:33:59Z","timestamp":1788521639295,"version":"build-2803163510"},"reference-count":32,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T00:00:00Z","timestamp":1726012800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Historical seroprevalence data for Anisakis in Spain vary greatly depending on the sampling region owing to different fish consumption habits. As a result of European Regulation (EC) No. 853\/2004, the Royal Decree 1420\/2006 on the prevention of parasitosis by Anisakis in fishery products supplied by establishments that serve food to final consumers or to communities came into force in Spain. In this study, a prevalence study of Anisakis in Madrid has been conducted to verify the prophylactic effects of the application of the law. Sera from 500 blood donors from the Fundaci\u00f3n Jim\u00e9nez D\u00edaz University Hospital (Madrid\/2021\u20132023) were collected, and the levels of anti-Anisakis IgG, IgA, and IgE were analyzed by ELISA, comparing them with those obtained with 110 donors from the Red Cross and the \u201cG\u00f3mez Ulla\u201d Central Defense Hospital (Madrid\/2001\u20132002). The percentages of positivity in the 2021\u20132023 donor group were IgG (13.6%), IgA (13.6%), and IgE (2.2%), while in the 2001\u20132002 donors they were positive for IgG (15.45%), IgA (14.54%), and IgE (11.65%). A reduction of more than 80% was observed in the prevalence of anti-Anisakis IgE in the healthy population of Madrid, which confirmed the positive effect of RD1420\/2006, which was later incorporated into RD1021\/2022.<\/jats:p>","DOI":"10.3390\/pathogens13090782","type":"journal-article","created":{"date-parts":[[2024,9,11]],"date-time":"2024-09-11T02:34:49Z","timestamp":1726022089000},"page":"782","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Prevalence of Anisakiasis in Madrid (Spain) after 20 Years of Preventive Legislation"],"prefix":"10.3390","volume":"13","author":[{"given":"Eva","family":"Blanco-Costales","sequence":"first","affiliation":[{"name":"Unidad de Parasitolog\u00eda, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0022-316X","authenticated-orcid":false,"given":"Alejandra L.","family":"Gonz\u00e1lez-Quevedo","sequence":"additional","affiliation":[{"name":"Unidad de Parasitolog\u00eda, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Lorenzo-Bernardo","sequence":"additional","affiliation":[{"name":"Unidad de Parasitolog\u00eda, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda P.","family":"de la Hoz-Mart\u00edn","sequence":"additional","affiliation":[{"name":"Unidad de Parasitolog\u00eda, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Rodero","sequence":"additional","affiliation":[{"name":"Unidad de Parasitolog\u00eda, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Puente","sequence":"additional","affiliation":[{"name":"Unidad de Control de Calidad, Centro Militar de Farmacia de la Defensa, Base Log\u00edstica San Pedro, 28770 Colmenar Viejo, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Moreno-Torres","sequence":"additional","affiliation":[{"name":"Unidad de Enfermedades Desmielinizantes, Servicio de Neurolog\u00eda, Hospital Universitario Fundaci\u00f3n Jim\u00e9nez D\u00edaz, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7948-9889","authenticated-orcid":false,"given":"Carmen","family":"Cu\u00e9llar","sequence":"additional","affiliation":[{"name":"Unidad de Parasitolog\u00eda, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0306-8399","authenticated-orcid":false,"given":"Juan","family":"Gonz\u00e1lez-Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Unidad de Parasitolog\u00eda, Departamento de Microbiolog\u00eda y Parasitolog\u00eda, Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/S0065-308X(08)00202-9","article-title":"Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes","volume":"66","author":"Mattiucci","year":"2008","journal-title":"Adv. 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