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Excess nutrients are caused by intensive livestock and agricultural operations, pollution, and population growth, which in turn leads to uncontrolled microbiological development. This impairment in water quality can constitute a risk to animal, human, and environmental health. To thoroughly characterize the microbial communities, shotgun metagenomics was used to characterize the taxonomic and functional pattern of microorganisms that inhabit urban wetlands in the Los Lagos Region of Chile. The main objective was to identify microorganisms of veterinary relevance, assess their potential antibiotic resistance, and characterize the main virulence mechanism. As expected, a high diversity of microorganisms was identified, including bacteria described as animal or human pathogens, such as Pasteurella multocida, Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli. Also, a diverse repertory of antimicrobial-resistant genes (ARGs) was detected in metagenomic assembled sequences and inside the sequence of mobile genetic elements, genes that confer mainly resistance to beta-lactams, consistent with the families of antibiotics most used in Chile. In addition, a diverse collection of virulence mechanisms was also identified. Given the significance of the relationship between environmental, animal, and human health\u2014a concept known as One Health\u2014there is a need to establish molecular surveillance programs that monitor the environmental biohazard elements using molecular tools. This work is the first report of the presence of these harmful biological elements in urban wetlands subjected to anthropogenic pressure, located in the south of Chile.<\/jats:p>","DOI":"10.3390\/pathogens13090788","type":"journal-article","created":{"date-parts":[[2024,9,12]],"date-time":"2024-09-12T05:03:04Z","timestamp":1726117384000},"page":"788","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Metagenomics Insight into Veterinary and Zoonotic Pathogens Identified in Urban Wetlands of Los Lagos, Chile"],"prefix":"10.3390","volume":"13","author":[{"given":"Catherine","family":"Opitz-R\u00edos","sequence":"first","affiliation":[{"name":"Laboratorio Institucional, Universidad San Sebasti\u00e1n, Puerto Montt 5501842, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alvaro","family":"Burgos-Pacheco","sequence":"additional","affiliation":[{"name":"Escuela de Medicina Veterinaria, Facultad de Ciencias de la Naturaleza, Universidad San Sebasti\u00e1n, Puerto Montt 5501842, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francisca","family":"Paredes-C\u00e1rcamo","sequence":"additional","affiliation":[{"name":"Escuela de Medicina Veterinaria, Facultad de Ciencias de la Naturaleza, Universidad San Sebasti\u00e1n, Puerto Montt 5501842, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7358-409X","authenticated-orcid":false,"given":"Javier","family":"Campanini-Salinas","sequence":"additional","affiliation":[{"name":"Facultad de Medicina y Ciencia, Universidad San Sebasti\u00e1n, Puerto Montt 5501842, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8183-0043","authenticated-orcid":false,"given":"Daniel A.","family":"Medina","sequence":"additional","affiliation":[{"name":"Laboratorio Institucional, Universidad San Sebasti\u00e1n, Puerto Montt 5501842, Chile"},{"name":"Escuela de Medicina Veterinaria, Facultad de Ciencias de la Naturaleza, Universidad San Sebasti\u00e1n, Puerto Montt 5501842, Chile"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/21513732.2015.1006250","article-title":"Ecosystem Services of Wetlands","volume":"11","author":"Mitsch","year":"2015","journal-title":"Int. J. Biodivers. Sci. Eco. Srvcs. Mgmt."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"114086","DOI":"10.1016\/j.envpol.2020.114086","article-title":"Quantitative Study on Redistribution of Nitrogen and Phosphorus by Wetland Plants under Different Water Quality Conditions","volume":"261","author":"Xing","year":"2020","journal-title":"Environ. Pollut."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"122417","DOI":"10.1016\/j.envpol.2023.122417","article-title":"Phytosphere Purification of Urban Domestic Wastewater","volume":"336","author":"Qu","year":"2023","journal-title":"Environ. Pollut."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"13835","DOI":"10.1038\/ncomms13835","article-title":"Carbon Storage in US Wetlands","volume":"7","author":"Nahlik","year":"2016","journal-title":"Nat. Commun."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zamora, S., Zit\u00e1cuaro-Contreras, I., Betanzo-Torres, E.A., Herazo, L.C.S., Sandoval-Herazo, M., Vidal-\u00c1lvarez, M., and Mar\u00edn-Mu\u00f1iz, J.L. (2022). Carbon Pool in Mexican Wetland Soils: Importance of the Environmental Service. Life, 12.","DOI":"10.3390\/life12071032"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"620","DOI":"10.1071\/MF17019","article-title":"Global Extent and Distribution of Wetlands: Trends and Issues","volume":"69","author":"Davidson","year":"2018","journal-title":"Mar. Freshw. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"112160","DOI":"10.1016\/j.jenvman.2021.112160","article-title":"Impact of Climate Change on Wetland Ecosystems: A Critical Review of Experimental Wetlands","volume":"286","author":"Salimi","year":"2021","journal-title":"J. Environ. Manag."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Gardner, R.C., and Davidson, N.C. (2011). The Ramsar Convention. Wetlands, Springer.","DOI":"10.1007\/978-94-007-0551-7_11"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1126\/science.287.5452.443","article-title":"Emerging Infectious Diseases of Wildlife\u2014Threats to Biodiversity and Human Health","volume":"287","author":"Daszak","year":"2000","journal-title":"Science"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"983","DOI":"10.1098\/rstb.2001.0888","article-title":"Risk Factors for Human Disease Emergence","volume":"356","author":"Taylor","year":"2001","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1988","DOI":"10.12688\/f1000research.17649.2","article-title":"Epidemiology of Zoonotic Tick-Borne Diseases in Latin America: Are We Just Seeing the Tip of the Iceberg?","volume":"7","year":"2019","journal-title":"F1000Research"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"19","DOI":"10.1186\/s40693-014-0019-x","article-title":"High Prevalence of Pathogenic Leptospira in Alien American Mink (Neovison Vison) in Patagonia","volume":"87","author":"Barros","year":"2014","journal-title":"Rev. Chil. Hist. Nat."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1136\/vr.g143","article-title":"The Evolution of One Health: A Decade of Progress and Challenges for the Future","volume":"174","author":"Gibbs","year":"2014","journal-title":"Vet. Rec."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"145","DOI":"10.20506\/rst.38.1.2949","article-title":"Importance of a One Health Approach in Advancing Global Health Security and the Sustainable Development Goals","volume":"38","author":"Sinclair","year":"2019","journal-title":"Rev. Sci. Tech."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Riley, L.W., and Blanton, R.E. (2018). Advances in Molecular Epidemiology of Infectious Diseases: Definitions, Approaches, and Scope of the Field. Microbiol. Spectr., 6.","DOI":"10.1128\/microbiolspec.AME-0001-2018"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1038\/nature06536","article-title":"Global Trends in Emerging Infectious Diseases","volume":"451","author":"Jones","year":"2008","journal-title":"Nature"},{"key":"ref_17","unstructured":"(2024, July 16). Taking a Multisectoral, One Health Approach: A Tripartite Guide to Addressing Zoonotic Diseases in Countries. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789241514934."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Fresia, P., Antelo, V., Salazar, C., Gim\u00e9nez, M., D\u2019Alessandro, B., Afshinnekoo, E., Mason, C., Gonnet, G.H., and Iraola, G. (2019). Urban Metagenomics Uncover Antibiotic Resistance Reservoirs in Coastal Beach and Sewage Waters. Microbiome, 7.","DOI":"10.1186\/s40168-019-0648-z"},{"key":"ref_19","first-page":"47","article-title":"Identification of Potentially Harmful Bacterial Genera of Veterinary Relevance in the Llanquihue Urban Wetlands","volume":"56","author":"Rocha","year":"2024","journal-title":"Austral J. Vet. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Campanini-Salinas, J., Opitz-R\u00edos, C., Sagredo-Mella, J., Contreras, S.D., Gimenez, M., Paez, P.A., Tarifa, M.C., Concha-Rubio, N.D., and Medina, D.A. (2024). Antimicrobial Resistance Elements in Coastal Water Identified from Llanquihue Lake, Chile. Biol. Life Sci.","DOI":"10.20944\/preprints202406.0310.v1"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"787","DOI":"10.1038\/nrmicro3565","article-title":"Twenty Years of Bacterial Genome Sequencing","volume":"13","author":"Loman","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Marchesi, J.R., and Ravel, J. (2015). The Vocabulary of Microbiome Research: A Proposal. Microbiome, 3.","DOI":"10.1186\/s40168-015-0094-5"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2350","DOI":"10.1128\/JCM.37.7.2350-2351.1999","article-title":"Inactivation of Mycobacterium tuberculosis for DNA Typing Analysis","volume":"37","author":"Drugeon","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_24","unstructured":"(2024, July 16). Babraham Bioinformatics\u2014FastQC a Quality Control Tool for High Throughput Sequence Data. Available online: https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2114","DOI":"10.1093\/bioinformatics\/btu170","article-title":"Trimmomatic: A Flexible Trimmer for Illumina Sequence Data","volume":"30","author":"Bolger","year":"2014","journal-title":"Bioinformatics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.1923","article-title":"Fast Gapped-Read Alignment with Bowtie 2","volume":"9","author":"Langmead","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1674","DOI":"10.1093\/bioinformatics\/btv033","article-title":"MEGAHIT: An Ultra-Fast Single-Node Solution for Large and Complex Metagenomics Assembly via Succinct de Bruijn Graph","volume":"31","author":"Li","year":"2015","journal-title":"Bioinformatics"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: Quality Assessment Tool for Genome Assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Wood, D.E., Lu, J., and Langmead, B. (2019). Improved Metagenomic Analysis with Kraken 2. Genome Biol., 20.","DOI":"10.1186\/s13059-019-1891-0"},{"key":"ref_30","unstructured":"Seemann, T. (2019). Abricate: Mag_Right: Mass Screening of Contigs for Antimicrobial and Virulence Genes, Github."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2640","DOI":"10.1093\/jac\/dks261","article-title":"Identification of Acquired Antimicrobial Resistance Genes","volume":"67","author":"Zankari","year":"2012","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Feldgarden, M., Brover, V., Haft, D.H., Prasad, A.B., Slotta, D.J., Tolstoy, I., Tyson, G.H., Zhao, S., Hsu, C.-H., and McDermott, P.F. (2019). Validating the AMRFinder Tool and Resistance Gene Database by Using Antimicrobial Resistance Genotype-Phenotype Correlations in a Collection of Isolates. Antimicrob. Agents Chemother., 63.","DOI":"10.1128\/AAC.00483-19"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"D694","DOI":"10.1093\/nar\/gkv1239","article-title":"VFDB 2016: Hierarchical and Refined Dataset for Big Data Analysis\u201410 Years On","volume":"44","author":"Chen","year":"2016","journal-title":"Nucleic Acids Res."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Gim\u00e9nez, M., Ferr\u00e9s, I., and Iraola, G. (2022). Improved Detection and Classification of Plasmids from Circularized and Fragmented Assemblies. bioRxiv.","DOI":"10.1101\/2022.08.04.502827"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"D195","DOI":"10.1093\/nar\/gky1050","article-title":"PLSDB: A Resource of Complete Bacterial Plasmids","volume":"47","author":"Galata","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_36","unstructured":"R Core Team (2024, May 27). R A Language and Environment for Statistical Computing. R Foundation for Statistical Computing, Vienna.\u2014References\u2014Scientific Research Publishing. Available online: https:\/\/www.scirp.org\/reference\/referencespapers?referenceid=3131254."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Irizarry, R.A. (2024). Ggplot2. Introduction to Data Science, Chapman and Hall\/CRC.","DOI":"10.1201\/9781003220923-8"},{"key":"ref_38","unstructured":"(2024, July 16). Enfermedades de Denuncia Obligatoria (EDO) al SAG. Available online: https:\/\/www.sag.gob.cl\/ambitos-de-accion\/enfermedades-de-denuncia-obligatoria-edo-al-sag."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"203","DOI":"10.2174\/1874285801711010203","article-title":"Genetic Investigation of Beta-Lactam Associated Antibiotic Resistance among Escherichia coli Strains Isolated from Water Sources","volume":"11","author":"Ranjbar","year":"2017","journal-title":"Open Microbiol. J."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1128\/AAC.01574-08","article-title":"OqxAB Encoding a Multidrug Efflux Pump in Human Clinical Isolates of Enterobacteriaceae","volume":"53","author":"Kim","year":"2009","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2716","DOI":"10.1128\/AAC.49.7.2716-2719.2005","article-title":"New Lnu (C) Gene Conferring Resistance to Lincomycin by Nucleotidylation in Streptococcus Agalactiae UCN36","volume":"49","author":"Achard","year":"2005","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1952","DOI":"10.1128\/AEM.02159-06","article-title":"Erythromycin Resistance-Conferring Plasmid PRSB105, Isolated from a Sewage Treatment Plant, Harbors a New Macrolide Resistance Determinant, an Integron-Containing Tn 402 -like Element, and a Large Region of Unknown Function","volume":"73","author":"Szczepanowski","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"a025387","DOI":"10.1101\/cshperspect.a025387","article-title":"Tetracycline Antibiotics and Resistance","volume":"6","author":"Grossman","year":"2016","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"900","DOI":"10.1073\/pnas.0812143106","article-title":"Localized and Efficient Curli Nucleation Requires the Chaperone-like Amyloid Assembly Protein CsgF","volume":"106","author":"Nenninger","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Mackenzie, J.S., and Jeggo, M. (2019). The One Health Approach\u2014Why Is It so Important?. Trop. Med. Infect. Dis., 4.","DOI":"10.3390\/tropicalmed4020088"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"R\u00edos-Castro, R., Romero, A., Aranguren, R., Pallavicini, A., Banchi, E., Novoa, B., and Figueras, A. (2021). High-Throughput Sequencing of Environmental DNA as a Tool for Monitoring Eukaryotic Communities and Potential Pathogens in a Coastal Upwelling Ecosystem. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.765606"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Salazar, C., Gim\u00e9nez, M., Riera, N., Parada, A., Puig, J., Galiana, A., Grill, F., Vieytes, M., Mason, C.E., and Antelo, V. (2022). Human Microbiota Drives Hospital-Associated Antimicrobial Resistance Dissemination in the Urban Environment and Mirrors Patient Case Rates. Microbiome, 10.","DOI":"10.1186\/s40168-022-01407-8"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Ballesteros, N., P\u00e1ez, L., Luna, N., Reina, A., Urrea, V., S\u00e1nchez, C., Ram\u00edrez, A., Ramirez, J.D., and Mu\u00f1oz, M. (2023). Characterization of Microbial Communities in Seven Wetlands with Different Anthropogenic Burden Using Next Generation Sequencing in Bogot\u00e1, Colombia. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-42970-w"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"105587","DOI":"10.1016\/j.meegid.2024.105587","article-title":"German Coasts Harbor Non-O1\/Non-O139 Vibrio Cholerae with Clinical Virulence Gene Profiles","volume":"120","author":"Zhang","year":"2024","journal-title":"Infect. Genet. Evol."},{"key":"ref_50","first-page":"71","article-title":"Risk Assessment of Pseudomonas Aeruginosa in Water","volume":"Volume 201","author":"Mena","year":"2009","journal-title":"Reviews of Environmental Contamination and Toxicology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"662","DOI":"10.4315\/0362-028X-61.6.662","article-title":"Survival of Enterohemorrhagic Escherichia coli O157:H7 in Water","volume":"61","author":"Wang","year":"1998","journal-title":"J. Food Prot."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"109541","DOI":"10.1016\/j.cclet.2024.109541","article-title":"Fluorescence Analysis of Antibiotics and Antibiotic-Resistance Genes in the Environment: A Mini Review","volume":"35","author":"Li","year":"2024","journal-title":"Chin. Chem. Lett."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1289\/ehp.1206446","article-title":"Management Options for Reducing the Release of Antibiotics and Antibiotic Resistance Genes to the Environment","volume":"121","author":"Pruden","year":"2013","journal-title":"Environ. Health Perspect."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1016\/j.chemosphere.2008.11.086","article-title":"Antibiotics in the Aquatic Environment\u2014A Review\u2014Part I","volume":"75","year":"2009","journal-title":"Chemosphere"},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"Katusiime, J., and Sch\u00fctt, B. (2020). Integrated Water Resources Management Approaches to Improve Water Resources Governance. Water, 12.","DOI":"10.3390\/w12123424"},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Manyi-Loh, C., Mamphweli, S., Meyer, E., and Okoh, A. (2018). Antibiotic Use in Agriculture and Its Consequential Resistance in Environmental Sources: Potential Public Health Implications. Molecules, 23.","DOI":"10.3390\/molecules23040795"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Pepi, M., and Focardi, S. (2021). Antibiotic-Resistant Bacteria in Aquaculture and Climate Change: A Challenge for Health in the Mediterranean Area. Int. J. Environ. Res. Public Health, 18.","DOI":"10.3390\/ijerph18115723"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2354","DOI":"10.1016\/j.jenvman.2009.01.023","article-title":"The Presence of Pharmaceuticals in the Environment Due to Human Use\u2014Present Knowledge and Future Challenges","volume":"90","year":"2009","journal-title":"J. Environ. Manag."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"530","DOI":"10.2166\/wp.2017.184","article-title":"Evidence-Based Decision-Making on Water Quality in Domestic Water Supply in Malawi, Ecuador, and Brazil","volume":"20","author":"Amjad","year":"2018","journal-title":"Water Policy"},{"key":"ref_60","unstructured":"(2024, July 17). Guidelines for Drinking-Water Quality, 4th Edition, Incorporating the 1st Addendum. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789241549950."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/9\/788\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:54:29Z","timestamp":1760111669000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/9\/788"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,12]]},"references-count":60,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["pathogens13090788"],"URL":"https:\/\/doi.org\/10.3390\/pathogens13090788","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,12]]}}}