{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T18:36:33Z","timestamp":1787164593797,"version":"build-2736575974"},"reference-count":38,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T00:00:00Z","timestamp":1726444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Office of the Vice-President for Research, Iowa State University"},{"name":"DBT Star College Scheme"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The present study aimed to investigate pathological epidemiology and molecular confirmation of Ehrlichia canis among pet dogs in Bhubaneswar, Odisha, a state in eastern India. A total of 178 dogs were screened for Ehrlichiosis based on history, clinical signs, blood, and buffy coat smear examination, resulting in only 56 dogs (31.46%) screening positive. The epidemiological study recorded a non-significant (p \u2265 0.05) increase in incidences among male dogs (68%), German Shepherds (25%), dogs more than 20 kg body weight (75%), in the summer months (55%), and dogs housed in pukka houses with exposure to the outside (59%). The majority of the infected dogs had a history of tick infestation (79%) at some point in their lives. Clinical signs showed non-typical manifestations like fever, lethargy, diarrhoea, epistaxis, hind limb edema, and corneal opacity. Haematological studies revealed anaemia and thrombocytopenia along with neutrophilia with relative lymphopenia and monocytosis. A decreasing trend was observed in the levels of total protein and albumin, with an increase in the levels of globulin, alanine aminotransferase, alanine aminotransferase, aspartate aminotransferase, blood urea nitrogen, and creatinine. The ultrasonography studies revealed hepatosplenomegaly along with hyper-echogenicity in various organs. Proteinuria and haematuria were consistent, along with the presence of bile salts in the urine of affected dogs. Molecular confirmation from n-type PCR data using Ehrlichia-specific primers targeting the p28 gene (843 bp) was done, and the identified gene sequences submitted to NCBI databases have accession numbers OQ383671-OQ383674 and OP886674-OP886677. Ticks collected from dogs were identified morphologically through microscopy and scanning electron microscopy as Rhipicephalus sanguineus.<\/jats:p>","DOI":"10.3390\/pathogens13090803","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T11:36:37Z","timestamp":1726486597000},"page":"803","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Molecular Confirmation, Epidemiology, and Pathophysiology of Ehrlichia canis Prevalence in Eastern India"],"prefix":"10.3390","volume":"13","author":[{"given":"Ankita","family":"Chakraborty","sequence":"first","affiliation":[{"name":"Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar 751003, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prasana Kumar","family":"Rath","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar 751003, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susen Kumar","family":"Panda","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar 751003, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bidyut Prava","family":"Mishra","sequence":"additional","affiliation":[{"name":"Department of Livestock Products Technology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology, Bhubaneswar 751003, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2491-4045","authenticated-orcid":false,"given":"Manaswini","family":"Dehuri","sequence":"additional","affiliation":[{"name":"Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology (OUAT), Bhubaneswar 751003, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sangram","family":"Biswal","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Odisha University of Agriculture and Technology (OUAT), Bhubaneswar 751003, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3847-8539","authenticated-orcid":false,"given":"Manoj Kumar","family":"Jena","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, School of Bioengineering and Biosciences, Lovely Professional University, Phagwara 144411, Punjab, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basanta Pravas","family":"Sahu","sequence":"additional","affiliation":[{"name":"School of Biological Sciences, The University of Hong Kong, Pok Fu Lam, Hong Kong 999077, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5285-6578","authenticated-orcid":false,"given":"Biswaranjan","family":"Paital","sequence":"additional","affiliation":[{"name":"Redox Regulation Laboratory, Department of Zoology, College of Basic Science and Humanities, Odisha University of Agriculture and Technology, Bhubaneswar 751003, Odisha, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3748-3395","authenticated-orcid":false,"given":"Dipak Kumar","family":"Sahoo","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,16]]},"reference":[{"key":"ref_1","first-page":"418","article-title":"Existence en Alg\u00e9rie d\u2019une Ricketttsia du chien","volume":"28","author":"Donatien","year":"1935","journal-title":"Bull. Soc. Pathol. Exot."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"117","DOI":"10.29261\/pakvetj\/2020.061","article-title":"Prevalence and first molecular characterization of Ehrlichia canis in Egyptian dogs","volume":"41","author":"Selim","year":"2020","journal-title":"Pak. Vet. J."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"14","DOI":"10.11606\/S1413-95962011000100002","article-title":"Soropreval\u00eancia e fatores de risco associados \u00e0 soropositividade para Ehrlichia canis em c\u00e3es do semi\u00e1rido da Para\u00edba","volume":"48","author":"Azevedo","year":"2011","journal-title":"Braz. J. Oveterinary Res. Anim. Sci."},{"key":"ref_4","first-page":"1","article-title":"Anti-Ehrlichia canis antibodies detection by \u201cDot-ELISA\u201d in naturally infected dogs","volume":"9","author":"Oliveira","year":"2000","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_5","first-page":"578","article-title":"A retrospective study of dogs infected with Ehrlichia canis from 2017\u20132019 in the Thonburi area of Bangkok province, Thailand","volume":"9","author":"Lorsirigool","year":"2020","journal-title":"Int. J. Vet. Sci."},{"key":"ref_6","first-page":"2","article-title":"Renal histopathological changes in dogs naturally infected with Ehrlichia cani","volume":"9","author":"Silva","year":"2016","journal-title":"Braz. J. Vet. Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1007\/s00580-006-0637-z","article-title":"Canine dirofilariasis and concurrent tick-borne transmitted diseases in Bangkok, Thailand","volume":"15","author":"Niwetpathomwat","year":"2006","journal-title":"Comp. Clin. Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1","DOI":"10.14202\/vetworld.2022.1-9","article-title":"Retrospective analysis of canine monocytic ehrlichiosis in Thailand with emphasis on hematological and ultrasonographic changes","volume":"15","author":"Angkanaporn","year":"2022","journal-title":"Vet. World"},{"key":"ref_9","first-page":"119","article-title":"Pathology of canine monocytic ehrlichiosis in a German Shepherd dog","volume":"40","author":"Lakkawar","year":"2003","journal-title":"Slov. Vet. Res."},{"key":"ref_10","first-page":"12","article-title":"Canine monocytic ehrlichiosis\u2014from pathology to clinical manifestations","volume":"68","author":"Waner","year":"2013","journal-title":"Isr. J. Vet. Med."},{"key":"ref_11","first-page":"282","article-title":"Comparative diagnostic methods for canine ehrlichiosis","volume":"37","author":"Parmar","year":"2013","journal-title":"Turk. J. Vet. Anim. Sci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1007\/s12639-016-0860-8","article-title":"Molecular prevalence and haemato-biochemical profile of canine monocytic ehrlichiosis in dogs in and around Hisar, Haryana, India","volume":"41","author":"Bai","year":"2017","journal-title":"J. Parasit. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1590\/S0103-84782008000300027","article-title":"Canine ehrlichiosis: Clinical, haematological, serological and molecular aspects","volume":"38","author":"Nakaghi","year":"2008","journal-title":"Cienc. Rural"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1590\/S1984-29612010000200001","article-title":"Sensitivity evaluation of a single-step PCR assay using Ehrlichia canis p28 gene as a target and its application in diagnosis of canine ehrlichiosis","volume":"19","author":"Nakaghi","year":"2010","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.tvjl.2006.11.002","article-title":"Sero-prevalence and risk indicators for Canine Ehrlichiosis in three rural areas of Brazil","volume":"174","author":"Costa","year":"2007","journal-title":"Vet. J."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1590\/S1984-29612015030","article-title":"Canine ehrlichiosis: Prevalence and epidemiology in northeast Brazil","volume":"24","author":"Guedes","year":"2015","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"129","DOI":"10.17221\/7380-VETMED","article-title":"Molecular prevalence and risk factors for the occurrence of canine monocytic ehrlichiosis","volume":"59","author":"Milanjeet","year":"2014","journal-title":"Vet. Med."},{"key":"ref_19","first-page":"63","article-title":"Rickettsia canis in Hyderabad","volume":"35","author":"Okubanjo","year":"2014","journal-title":"Indian Vet. J."},{"key":"ref_20","first-page":"138","article-title":"Therapeutic management of acute canine monocytic ehrlichiosis","volume":"90","author":"Bhardwaj","year":"2013","journal-title":"Indian Vet. J."},{"key":"ref_21","first-page":"923","article-title":"Prevalence and Molecular Characterization of Ehrlichia canis in dogs of Jammu region","volume":"8","author":"Himalini","year":"2018","journal-title":"J. Anim. Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"713","DOI":"10.14202\/vetworld.2015.713-717","article-title":"Hematobiochemical changes in ehrlichiosis in dogs of Anand region, Gujarat","volume":"8","author":"Bhadesiya","year":"2015","journal-title":"Vet. World"},{"key":"ref_23","first-page":"24","article-title":"Carrapato em c\u00e3es no Brasil","volume":"6","author":"Labruna","year":"2001","journal-title":"Clin. Vet."},{"key":"ref_24","unstructured":"O\u2019Dwyer, L.H. (2000). Diagno\u2019stico de Hemoparasitos e Carrapatos de C\u00e3es Procedentes de A\u2019reas Rurais Procedentes de Tr\u00eas Mesorregi\u00f5es do Estado do Rio de Janeiro, Brasil. [Ph.D. Thesis, Universidade Federal Rural do Rio de Janeiro-UFRRJ]."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/1756-3305-3-26","article-title":"Biology and ecology of the brown dog tick, Rhipicephalus sanguineus","volume":"3","year":"2010","journal-title":"Parasites Vectors"},{"key":"ref_26","first-page":"8","article-title":"Monocytic ehrlichiosis in Aegean region dogs: Clinical and haematological findings","volume":"14","author":"Tuna","year":"2019","journal-title":"Ataturk Univ. Vet. Bilim. Derg."},{"key":"ref_27","first-page":"260","article-title":"Clinical and hemato-biochemical studies on ehrlichiosis and babesiosis in canines","volume":"19","author":"Haritha","year":"2018","journal-title":"Intas Polivet"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1007\/s00580-008-0759-6","article-title":"Clinicopathological study of experimentally induced canine monocytic ehrlichiosis","volume":"18","author":"Rungsipipat","year":"2009","journal-title":"Comp. Clin. Pathol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2388","DOI":"10.14202\/vetworld.2020.2388-2394","article-title":"Hematological profile of blood parasitic infected dogs in Southern Thailand","volume":"13","author":"Thongsahuan","year":"2020","journal-title":"Vet. World"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1016\/j.vetmic.2007.05.021","article-title":"Sequence and phylogenetic analysis of the 16S rRNA gene of Ehrlichia canis strains in dogs with clinical monocytic ehrlichiosis","volume":"125","author":"Siarkou","year":"2007","journal-title":"Vet. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1093\/infdis\/130.4.357","article-title":"Tropical canine pancytopenia: Clinical, hematologic, and serologic response of dogs to Ehrlichia canis infection, tetracycline therapy and challenge inoculation","volume":"130","author":"Buhles","year":"1974","journal-title":"J. Infect. Dis."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"255","DOI":"10.14202\/vetworld.2017.255-261","article-title":"Clinical and haematobiochemical response in canine monocytic ehrlichiosis seropositive dogs of Punjab","volume":"10","author":"Kottadamane","year":"2017","journal-title":"Vet. World"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"101909","DOI":"10.1016\/j.ttbdis.2022.101909","article-title":"Comparative genomic analysis of the first Ehrlichia canis detections in Australia","volume":"13","author":"Neave","year":"2022","journal-title":"Ticks Tick Borne Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1590\/S1984-29612011000100015","article-title":"Ehrlichia canis (Jaboticabal Strain) induces the expression of TNF-\u03b1 in leukocytes and splenocytes of experimentally infected dogs","volume":"20","author":"Faria","year":"2011","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1111\/j.1939-1676.2010.0501.x","article-title":"Severe hepatitis associated with acute Ehrlichia canis infection in a dog","volume":"24","author":"Mylonakis","year":"2010","journal-title":"J. Vet. Intern. Med."},{"key":"ref_36","first-page":"148","article-title":"Abdominal Ultrasonography of Dogs with Canine Monocytic Ehrlichiosis","volume":"89","author":"Behera","year":"2012","journal-title":"Indian Vet. J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"672107","DOI":"10.1155\/2012\/672107","article-title":"Diagnostic imaging of canine hepatobiliary affections: A review","volume":"2012","author":"Kumar","year":"2012","journal-title":"Vet. Med. Int."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.tvjl.2019.01.015","article-title":"An update on the treatment of canine monocytic ehrlichiosis (Ehrlichia canis)","volume":"246","author":"Mylonakis","year":"2019","journal-title":"Vet. J."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/9\/803\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T15:57:33Z","timestamp":1760111853000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/9\/803"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,9,16]]},"references-count":38,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2024,9]]}},"alternative-id":["pathogens13090803"],"URL":"https:\/\/doi.org\/10.3390\/pathogens13090803","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,9,16]]}}}