{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:52:31Z","timestamp":1791244351317,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2024,9,23]],"date-time":"2024-09-23T00:00:00Z","timestamp":1727049600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Japan Agency of Medical Research and Development (AMED)","award":["JP223fa627004"],"award-info":[{"award-number":["JP223fa627004"]}]},{"name":"Japan Agency of Medical Research and Development (AMED)","award":["JP243fa627004"],"award-info":[{"award-number":["JP243fa627004"]}]},{"name":"Japan Agency of Medical Research and Development (AMED)","award":["JP24fk0108656"],"award-info":[{"award-number":["JP24fk0108656"]}]},{"name":"Japan Agency of Medical Research and Development (AMED)","award":["P24fk0108656"],"award-info":[{"award-number":["P24fk0108656"]}]},{"name":"Japan Agency of Medical Research and Development (AMED)","award":["23jm0210079"],"award-info":[{"award-number":["23jm0210079"]}]},{"name":"Japan Agency of Medical Research and Development (AMED)","award":["24K10246"],"award-info":[{"award-number":["24K10246"]}]},{"name":"Japan Agency of Medical Research and Development (AMED)","award":["24K02288"],"award-info":[{"award-number":["24K02288"]}]},{"name":"Japanese Society of Promotion and Science (JSPS)","award":["JP223fa627004"],"award-info":[{"award-number":["JP223fa627004"]}]},{"name":"Japanese Society of Promotion and Science (JSPS)","award":["JP243fa627004"],"award-info":[{"award-number":["JP243fa627004"]}]},{"name":"Japanese Society of Promotion and Science (JSPS)","award":["JP24fk0108656"],"award-info":[{"award-number":["JP24fk0108656"]}]},{"name":"Japanese Society of Promotion and Science (JSPS)","award":["P24fk0108656"],"award-info":[{"award-number":["P24fk0108656"]}]},{"name":"Japanese Society of Promotion and Science (JSPS)","award":["23jm0210079"],"award-info":[{"award-number":["23jm0210079"]}]},{"name":"Japanese Society of Promotion and Science (JSPS)","award":["24K10246"],"award-info":[{"award-number":["24K10246"]}]},{"name":"Japanese Society of Promotion and Science (JSPS)","award":["24K02288"],"award-info":[{"award-number":["24K02288"]}]},{"name":"Sustainable Development Goals (SDGs) research grant of Shimane University","award":["JP223fa627004"],"award-info":[{"award-number":["JP223fa627004"]}]},{"name":"Sustainable Development Goals (SDGs) research grant of Shimane University","award":["JP243fa627004"],"award-info":[{"award-number":["JP243fa627004"]}]},{"name":"Sustainable Development Goals (SDGs) research grant of Shimane University","award":["JP24fk0108656"],"award-info":[{"award-number":["JP24fk0108656"]}]},{"name":"Sustainable Development Goals (SDGs) research grant of Shimane University","award":["P24fk0108656"],"award-info":[{"award-number":["P24fk0108656"]}]},{"name":"Sustainable Development Goals (SDGs) research grant of Shimane University","award":["23jm0210079"],"award-info":[{"award-number":["23jm0210079"]}]},{"name":"Sustainable Development Goals (SDGs) research grant of Shimane University","award":["24K10246"],"award-info":[{"award-number":["24K10246"]}]},{"name":"Sustainable Development Goals (SDGs) research grant of Shimane University","award":["24K02288"],"award-info":[{"award-number":["24K02288"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Dengue is a viral infection caused by the dengue virus (DENV), transmitted to humans through the bite of infected Aedes mosquitoes. About half of the world\u2019s population is now at risk of dengue, which represents a global public health concern, especially in tropical and subtropical countries. Early detection of the viral infection is crucial to manage the disease; hence, effective rapid diagnostic tests are essential. In this study, we evaluated the performance between the new Fujifilm Dengue non-structural antigen diagnosis kit (FF NS1 kit) and the SD Bioline NS1 antigen test kit (SD NS1 kit) against the quantitative real-time polymerase chain reaction (qRT-PCR) assays. The 140 acute serum samples collected from the Yangon General Hospital and Yangon Children\u2019s Hospital, Myanmar, from 2017 to 2019 were characterised by the three assays. With the qRT-PCR as the standard, the FF NS1 kit and the SD NS1 kit exhibited sensitivity of 94.3% and 88.6%, respectively, and specificity of 100% in both kits. Moreover, the positivity rates of the FF NS1 kit and the SD NS1 kit were 97.5% and 95% in primary infection and 90% and 80% in secondary infection, respectively. Our overall results suggest that the FF NS1 kit is reliable and accurate for detecting DENV infection.<\/jats:p>","DOI":"10.3390\/pathogens13090818","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T10:41:47Z","timestamp":1727174507000},"page":"818","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Performance of Fujifilm Dengue NS1 Antigen Rapid Diagnosis Kit Compared to Quantitative Real-Time Polymerase Chain Reaction"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8986-6307","authenticated-orcid":false,"given":"Mya Myat","family":"Ngwe Tun","sequence":"first","affiliation":[{"name":"Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"},{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"},{"name":"Center for Vaccines and Therapeutic Antibodies for Emerging Infectious Diseases, Shimane University, Izumo 690-8504, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Merveille","family":"Kapandji","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsuhiko","family":"Wada","sequence":"additional","affiliation":[{"name":"Medical Systems Research and Development Center, FUJIFILM Corporation, Tokyo 107-0052, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ko","family":"Yamamoto","sequence":"additional","affiliation":[{"name":"Medical Systems Research and Development Center, FUJIFILM Corporation, Tokyo 107-0052, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4072-0745","authenticated-orcid":false,"given":"Shyam Prakash","family":"Dumre","sequence":"additional","affiliation":[{"name":"Central Department of Microbiology, Tribhuvan University, Kathmandu 44601, Nepal"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khine Mya","family":"Nwe","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Htin","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Ministry of Health, Yangon 11191, Myanmar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-6706-438X","authenticated-orcid":false,"given":"Yuki","family":"Takamatsu","sequence":"additional","affiliation":[{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyaw Zin","family":"Thant","sequence":"additional","affiliation":[{"name":"Myanmar Academy of Medical Science, Yangon 11201, Myanmar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hlaing Myat","family":"Thu","sequence":"additional","affiliation":[{"name":"Department of Medical Research, Ministry of Health, Yangon 11191, Myanmar"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Urano","sequence":"additional","affiliation":[{"name":"Center for Vaccines and Therapeutic Antibodies for Emerging Infectious Diseases, Shimane University, Izumo 690-8504, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7992-7586","authenticated-orcid":false,"given":"Basu Dev","family":"Pandey","sequence":"additional","affiliation":[{"name":"DEJIMA Infectious Diseases Research Alliance, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8391-1157","authenticated-orcid":false,"given":"Kouichi","family":"Morita","sequence":"additional","affiliation":[{"name":"Department of Tropical Viral Vaccine Development, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"},{"name":"Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki 852-8523, Japan"},{"name":"Center for Vaccines and Therapeutic Antibodies for Emerging Infectious Diseases, Shimane University, Izumo 690-8504, Japan"},{"name":"DEJIMA Infectious Diseases Research Alliance, Nagasaki University, Nagasaki 852-8523, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s40506-023-00263-w","article-title":"Dengue: Update on Clinically Relevant Therapeutic Strategies and Vaccines","volume":"15","author":"Rathore","year":"2023","journal-title":"Curr. 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