{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,27]],"date-time":"2026-03-27T17:59:41Z","timestamp":1774634381087,"version":"3.50.1"},"reference-count":51,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T00:00:00Z","timestamp":1727308800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Slovak Grant Agency of Science","award":["VEGA 1\/0388\/22"],"award-info":[{"award-number":["VEGA 1\/0388\/22"]}]},{"name":"Slovak Grant Agency of Science","award":["VEGA 1\/0240\/23"],"award-info":[{"award-number":["VEGA 1\/0240\/23"]}]},{"name":"Slovak Grant Agency of Science","award":["APVV-19-0094"],"award-info":[{"award-number":["APVV-19-0094"]}]},{"name":"Slovak Grant Agency of Science","award":["APVV-21-0302"],"award-info":[{"award-number":["APVV-21-0302"]}]},{"name":"Slovak Research and Developmental Agency","award":["VEGA 1\/0388\/22"],"award-info":[{"award-number":["VEGA 1\/0388\/22"]}]},{"name":"Slovak Research and Developmental Agency","award":["VEGA 1\/0240\/23"],"award-info":[{"award-number":["VEGA 1\/0240\/23"]}]},{"name":"Slovak Research and Developmental Agency","award":["APVV-19-0094"],"award-info":[{"award-number":["APVV-19-0094"]}]},{"name":"Slovak Research and Developmental Agency","award":["APVV-21-0302"],"award-info":[{"award-number":["APVV-21-0302"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Fungal infections have become an important public health problem. Currently, there are only three available classes of antifungals for the treatment of invasive infections. Two of them, azoles and polyenes, target the synthesis of ergosterol or bind to sterols. A promising strategy to improve current therapies is the use of natural compounds in combinational therapies with the existing antifungals. In this work, we analyzed the changes in the susceptibility of the mutant strain of Nakaseomyces glabratus (Candida glabrata) lacking the ERG6 gene (encoding the sterol C-24 methyltransferase in ergosterol biosynthesis) in the presence of catechin and antifungal azoles. The reduced content of ergosterol in the Cgerg6\u0394 mutant resulted in the increased tolerance of the mutant cells to both azoles and polyenes. The combination of catechin with fluconazole or miconazole led to the growth inhibition of the azole-resistant Cgerg6\u0394 mutant strain. In the presence of catechin and miconazole, the Cgerg6\u0394 mutant fails to properly activate the expression of genes encoding the transcription factors CgYap1p and CgMsn4p, as well as the gene expression of CgCTA1, which are involved in oxidative stress response and lead to the intracellular accumulation of ROS. Finally, we show that catechin administration reduces mortality in a Galleria mellonella model infected with C. glabrata. Our work thus supports the use of catechin in combination therapies for fungal infections and shows that the CgERG6 gene could be a potential new drug target.<\/jats:p>","DOI":"10.3390\/pathogens13100834","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T09:46:20Z","timestamp":1727343980000},"page":"834","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Enhancement of ROS Production by Catechin Is a Primary Effect of Increased Azole Efficacy in Nakaseomyces glabratus (Candida glabrata) Cells Lacking the ERG6 Gene"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2784-1032","authenticated-orcid":false,"given":"Nora","family":"T\u00f3th Hervay","sequence":"first","affiliation":[{"name":"Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2913-2918","authenticated-orcid":false,"given":"Daniel","family":"Elia\u0161","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5300-3254","authenticated-orcid":false,"given":"Lucia","family":"\u010cern\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juraj","family":"Jacko","sequence":"additional","affiliation":[{"name":"Department of Nuclear Physics and Biophysics, Faculty of Mathematics, Physics and Informatics, Comenius University in Bratislava, Mlynska Dolina, 842 48 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcela","family":"Habov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nat\u00e1lia","family":"Chovancov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7516-8463","authenticated-orcid":false,"given":"Yvetta","family":"Gbelsk\u00e1","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovicova 6, 842 15 Bratislava, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,9,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15698\/mic2020.01.702","article-title":"The role of Lactobacillus species in the control of Candida via biotrophic interactions","volume":"7","author":"Zangl","year":"2019","journal-title":"Microb. 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