{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T05:02:31Z","timestamp":1783227751713,"version":"3.54.6"},"reference-count":47,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T00:00:00Z","timestamp":1729555200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"United States Department of Agriculture (USDA)"},{"name":"Plant Health Inspection Service (APHIS)"},{"name":"Global Partnership for Animal and Zoonotic Disease Surveillance (GPAZDS)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Marburg virus disease (MVD) is a zoonotic hemorrhagic disease with an estimated case fatality rate of up to 88%. Ghana recorded its first human MVD outbreak in June 2022 and although the outbreak was quickly brought under control, the transmission dynamics of the disease remained unclear. We assessed the presence of Marburg virus (MARV) antibodies in livestock and dogs and identified associated risk factors that increased the risk of these animals being exposed to MARV in five regions of Ghana. Sera collected from 3113 livestock and dogs in 2 climatic seasons (rainy and dry seasons) were tested for MARV antibodies using an indirect ELISA test. The samples were further tested using dot blotting to substantiate the presence of antibodies against MARV glycoprotein (GP). Overall, MARV antibodies were detected in 20.6% of the animals. The species-specific prevalence was 28.7% in cattle, 21.8% in sheep, 19.5% in goats, 15.3% in dogs and 11.2% in pigs. The seropositivity was higher in the rainy season [RR 1.5; 95% CI 1.3\u20131.8] and in older animals [RR = 2.6; 95% CI 1.9\u20133.4]. The findings underscore the importance of regular surveillance using the one health approach and future studies into the role of livestock and dogs as potential intermediaries in the circulation of MARV.<\/jats:p>","DOI":"10.3390\/pathogens13110917","type":"journal-article","created":{"date-parts":[[2024,10,22]],"date-time":"2024-10-22T04:10:14Z","timestamp":1729570214000},"page":"917","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Serological Evidence of Potential Marburg Virus Circulation in Livestock and Dogs in Ghana"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7092-7237","authenticated-orcid":false,"given":"Theophilus","family":"Odoom","sequence":"first","affiliation":[{"name":"Veterinary Services Department, Ministry of Food and Agriculture, Accra M-37, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0829-5774","authenticated-orcid":false,"given":"Sherry Ama Mawuko","family":"Johnson","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of Ghana, Accra LG-25, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2125-6382","authenticated-orcid":false,"given":"William","family":"Tasiame","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, School of Veterinary Medicine, Kwame Nkrumah University of Science and Technology, Kumasi 00233, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8133-0234","authenticated-orcid":false,"given":"Meyir Y.","family":"Ziekah","sequence":"additional","affiliation":[{"name":"Kumasi Zoological Gardens, Wildlife Division, Forestry Commission, Kumasi AK-181-1074, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph K.","family":"Abuh","sequence":"additional","affiliation":[{"name":"Veterinary Services Department, Ministry of Food and Agriculture, Accra M-37, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-1509-691X","authenticated-orcid":false,"given":"Benita","family":"Anderson","sequence":"additional","affiliation":[{"name":"School of Veterinary Medicine, University of Ghana, Accra LG-25, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fenteng","family":"Danso","sequence":"additional","affiliation":[{"name":"Veterinary Services Department, Ministry of Food and Agriculture, Accra M-37, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard K.","family":"Abbiw","sequence":"additional","affiliation":[{"name":"West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Accra LG-25, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franklin Y.","family":"Nuokpem","sequence":"additional","affiliation":[{"name":"West African Centre for Cell Biology of Infectious Pathogens, University of Ghana, Accra LG-25, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel","family":"Allegye-Cudjoe","sequence":"additional","affiliation":[{"name":"Veterinary Services Department, Ministry of Food and Agriculture, Accra M-37, Ghana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2489-0605","authenticated-orcid":false,"given":"Charles","family":"Lewis","sequence":"additional","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, National Bio and Agrodefense Facility, Animal and Plant Health Inspection Service, United States Department of Agriculture, Manhattan, KS 66506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bonto","family":"Faburay","sequence":"additional","affiliation":[{"name":"Foreign Animal Disease Diagnostic Laboratory, National Bio and Agrodefense Facility, Animal and Plant Health Inspection Service, United States Department of Agriculture, Manhattan, KS 66506, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"32761142","DOI":"10.1093\/database\/baaa062","article-title":"NCBI Taxonomy: A comprehensive update on curation, resources and tools","volume":"2020","author":"Schoch","year":"2020","journal-title":"Database"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"101123","DOI":"10.1016\/j.nmni.2023.101123","article-title":"Marburg Virus Disease in Tanzania: The most recent outbreak","volume":"53","author":"Deb","year":"2023","journal-title":"New Microbes New Infect."},{"key":"ref_3","unstructured":"WHO (2023, June 02). 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