{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T01:35:18Z","timestamp":1760146518508,"version":"build-2065373602"},"reference-count":75,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T00:00:00Z","timestamp":1731456000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Council for Scientific and Technological Development","award":["421867\/2018-3","311128\/2023-8"],"award-info":[{"award-number":["421867\/2018-3","311128\/2023-8"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Fast-growing mycobacteria cause difficult-to-treat infections due to their high intrinsic resistance to antibiotics as well as disinfectant agents. Mycobacterium abscessus complex (MAC) is the main cause of nontuberculous mycobacteria diseases. In this work, we evaluated the activity of the novel synthetic antimicrobial peptide, Agelaia-12, against Mycobacterium abscessus and M. massiliense. Agelaia-12 showed a minimum inhibitory concentration (MIC) of 25 \u03bcM detected against M. abscessus and M. massiliense with no cytotoxicity. The scanning electronic microscopy analysis of mycobacterial treated with Agelaia-12 demonstrated the presence of filamentous structures and aggregation of the cells. Congo red binding assay of M. abscessus exhibited altered dye accumulation after treatment with Agelaia-12. Treatment of M. abscessus- or M. massiliense-infected murine macrophages with Agelaia-12 decreased the mycobacterial load by 92% for the tested strains. Additionally, IFN-y KO mice infected with M. abscessus or M. massiliense and treated with Agelaia-12 showed a 98% reduction in lung bacterial load. Thus, the synthetic peptide Agelaia-12 may be a promising biomolecule for the treatment of mycobacteriosis, and its structural properties may serve as a foundational model for the design and development of novel pharmaceutical agents aimed at combating this disease.<\/jats:p>","DOI":"10.3390\/pathogens13110994","type":"journal-article","created":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T09:59:11Z","timestamp":1731491951000},"page":"994","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Novel Synthetic Peptide Agelaia-12 Has Improved Activity Against Mycobacterium abscessus Complex"],"prefix":"10.3390","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4033-1079","authenticated-orcid":false,"given":"Arthur Alves","family":"Coelho","sequence":"first","affiliation":[{"name":"Department of Biosciences and Technology, Tropical Pathology and Public Health Institute, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-050, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00edlia Cristina de Souza","family":"Barbosa","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Technology, Tropical Pathology and Public Health Institute, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-050, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adeliane Castro","family":"da Costa","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Technology, Tropical Pathology and Public Health Institute, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-050, GO, Brazil"},{"name":"Health Sciences Academic Unit, Federal University of Jata\u00ed, Jat\u00e1i 75801-615, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5950-106X","authenticated-orcid":false,"given":"Andr\u00e9","family":"Kipnis","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Technology, Tropical Pathology and Public Health Institute, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-050, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4200-6617","authenticated-orcid":false,"given":"Ana Paula","family":"Junqueira-Kipnis","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Technology, Tropical Pathology and Public Health Institute, Federal University of Goi\u00e1s, Goi\u00e2nia 74605-050, GO, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,11,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Luthra, S., Rominski, A., and Sander, P. 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