{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:16:04Z","timestamp":1792181764892,"version":"4.4.0"},"reference-count":56,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2024,12,7]],"date-time":"2024-12-07T00:00:00Z","timestamp":1733529600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Human papillomaviruses (HPVs) are responsible for the majority of sexually transmitted infections (STIs), some of which are oncogenic and can cause oropharyngeal or genital cancers. The HPV prevalence at the genital level varies according to the population studied but is higher in the seminal fluid of men suffering from idiopathic infertility than in the general population. The involvement of HPV in male infertility is supported by several studies suggesting that this virus can affect sperm quality by altering sperm DNA integrity, motility, number, viability, and morphology, and by inducing the production of anti-sperm antibodies (ASAs). HPVs may also have an impact on female fertility, mainly by increasing the risk of miscarriage and premature delivery and by altering the implantation of endometrial trophoblastic cells. In addition, an association with vaginal bacterial dysbiosis, notably involving Gardnerella vaginalis, or co-infection with an STI agent, serves as an aggravating factor. The aim of this review is to present current data on the potential role of HPVs in male and female infertility, along with data on infertility prevention and treatment strategies and the impact of vaccination in this context.<\/jats:p>","DOI":"10.3390\/pathogens13121076","type":"journal-article","created":{"date-parts":[[2024,12,9]],"date-time":"2024-12-09T04:01:37Z","timestamp":1733716897000},"page":"1076","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Impact of Human Papillomavirus (HPV) on Male and Female Fertility"],"prefix":"10.3390","volume":"13","author":[{"given":"Sara","family":"Chenafi-Adham","sequence":"first","affiliation":[{"name":"Department of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, 42023 Saint-Etienne, France"},{"name":"GIMAP Team 15, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, University of Saint-Etienne, University of Lyon, 42023 Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oulfa","family":"Boussetta-Charfi","sequence":"additional","affiliation":[{"name":"Department of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, 42023 Saint-Etienne, France"},{"name":"GIMAP Team 15, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, University of Saint-Etienne, University of Lyon, 42023 Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0882-3162","authenticated-orcid":false,"given":"Sylvie","family":"Pillet","sequence":"additional","affiliation":[{"name":"Department of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, 42023 Saint-Etienne, France"},{"name":"GIMAP Team 15, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, University of Saint-Etienne, University of Lyon, 42023 Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Bourlet","sequence":"additional","affiliation":[{"name":"Department of Infectious Agents and Hygiene, University Hospital of Saint-Etienne, 42023 Saint-Etienne, France"},{"name":"GIMAP Team 15, Centre International de Recherche en Infectiologie, INSERM U1111, CNRS, UMR5308, University of Saint-Etienne, University of Lyon, 42023 Saint-Etienne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.clinbiochem.2018.03.012","article-title":"Fertility and infertility: Definition and epidemiology","volume":"62","author":"Wyns","year":"2018","journal-title":"Clin. Biochem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1097\/MOU.0000000000001081","article-title":"Evaluation and Management of Male Genital Tract Infections in the Setting of Male Infertility: An Updated Review","volume":"33","author":"Rivero","year":"2023","journal-title":"Curr. Opin. Urol."},{"key":"ref_3","unstructured":"Nieschlag, S.M.A., Nieschlag, E., and Behre, H. (2013). Andrology: Male Reproductive Health and Dysfunction. Classification of Andrological Disorders, Springer Science & Business Media."},{"key":"ref_4","unstructured":"(2024, August 03). Arr\u00eat\u00e9 du 30 Juin 2017 Modifiant L\u2019arr\u00eat\u00e9 du 11 Avril 2008 Modifi\u00e9 Relatif Aux R\u00e8gles de Bonnes Pratiques Cliniques et Biologiques D\u2019assistance M\u00e9dicale \u00e0 la Procr\u00e9ation. 2017-Loi n\u00b02011-814 du 7 Juillet 2011 Relative \u00e0 la Bio\u00e9thique, Available online: https:\/\/www.legifrance.gouv.fr\/loda\/id\/JORFTEXT000035138350."},{"key":"ref_5","unstructured":"Boitrelle, F., Bourlet, T., and Grattard, F. (2022). Examen microbiologique du sperme. R\u00e9f\u00e9rentiel en Microbiologie M\u00e9dicale (R\u00e9mic), Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie. [7th ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.ejogrb.2008.03.009","article-title":"Genital tract infections and infertility","volume":"140","author":"Pellati","year":"2008","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol."},{"key":"ref_7","first-page":"492","article-title":"HIV, HPV and Chlamydia trachomatis: Impacts on male fertility","volume":"24","author":"Goulart","year":"2020","journal-title":"J. Bras. Reprod. Assist."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.fertnstert.2021.10.022","article-title":"Sperm quality and absence of SARS-CoV-2 RNA in semen after COVID-19 infection: A prospective, observational study and validation of the SpermCOVID test","volume":"117","author":"Donders","year":"2022","journal-title":"Fertil. Steril."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Tomaiuolo, R., Veneruso, I., Cariati, F., and D\u2019Argenio, V. (2020). Microbiota and Human Reproduction: The Case of Female Infertility. High-Throughput, 9.","DOI":"10.3390\/ht9020012"},{"key":"ref_10","first-page":"496","article-title":"Vaginal microbioma and the presence of Lactobacillus spp. As interferences in female fertility: A review system","volume":"27","author":"Monteiro","year":"2023","journal-title":"JBRA Assist. Reprod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e00450-20","DOI":"10.1128\/mSystems.00450-20","article-title":"Characterization of the Vaginal Microbiome in Women with Infertility and Its Potential Correlation with Hormone Stimulation during In Vitro Fertilization Surgery","volume":"5","author":"Zhao","year":"2020","journal-title":"mSystems"},{"key":"ref_12","first-page":"2632","article-title":"HPV infection in Europe","volume":"45","author":"Clifford","year":"2019","journal-title":"Eur. J. Cancer"},{"key":"ref_13","first-page":"329","article-title":"Correlation between human papillomavirus infection and reproduction","volume":"93","author":"Duan","year":"2022","journal-title":"Ginekol. Pol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1111\/jog.13188","article-title":"Prevalence of high-risk human papillomavirus infection in women with ovarian endometriosis","volume":"43","author":"Heidarpour","year":"2017","journal-title":"J. Obstet. Gynaecol. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"e1345","DOI":"10.1016\/S2214-109X(23)00305-4","article-title":"Global and regional estimates of genital human papillomavirus prevalence among men: A systematic review and meta-analysis","volume":"11","author":"Bruni","year":"2023","journal-title":"Lancet Glob. Health"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"578423","DOI":"10.1155\/2015\/578423","article-title":"Human papillomavirus infection, infertility, and assisted reproductive outcomes","volume":"2015","author":"Pereira","year":"2015","journal-title":"J. Pathog."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e13140","DOI":"10.1111\/and.13140","article-title":"Viral threat to male fertility","volume":"50","author":"Liu","year":"2018","journal-title":"Andrologia"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"209","DOI":"10.1093\/humrep\/dey348","article-title":"High-risk human papillomavirus in semen is associated with poor sperm progressive motility and a high sperm DNA fragmentation index in infertile men","volume":"34","author":"Boeri","year":"2019","journal-title":"Hum. Reprod."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Zullo, F., Fiano, V., Gillio-Tos, A., Leoncini, S., Nesi, G., Macr\u00ec, L., Preti, M., Rolfo, A., Benedetto, C., and Revelli, A. (2023). Human papillomavirus infection in women undergoing in-vitro fertilization: Effects on embryo development kinetics and live birth rate. Reprod. Biol. Endocrinol., 21.","DOI":"10.1186\/s12958-023-01091-9"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2832","DOI":"10.1093\/humrep\/deaa250","article-title":"20 years of the European IVF monitoring Consortium registry: What have we learned? A comparison with registries from two other regions","volume":"35","author":"Wyns","year":"2020","journal-title":"Hum. Reprod."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"791","DOI":"10.1002\/dc.21288","article-title":"The prevalence of abnormal cervical cytology in women with infertility","volume":"38","author":"AbdullGaffar","year":"2010","journal-title":"Diagn. Cytopathol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1236","DOI":"10.1016\/j.fertnstert.2019.02.001","article-title":"High-risk human papillomavirus infection in female and subsequent risk of infertility: A population-based cohort study","volume":"111","author":"Soylu","year":"2019","journal-title":"Fertil. Steril."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Hsu, L.C., Tsui, K.H., Cheng-Chung Wei, J., Tung, H., Hung, Y.M., and Chang, R. (2020). Female human papillomavirus infection associated with increased risk of infertility: A nationwide population based Cohort study. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17186505"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/j.rbmo.2019.10.019","article-title":"Human papillomavirus infection and female infertility: A systematic review and meta-analysis","volume":"40","author":"Yuan","year":"2020","journal-title":"Reprod. Biomed. Online"},{"key":"ref_25","first-page":"69","article-title":"Gardnerella, Trichomonas vaginalis, Candida, Chlamydia trachomatis, Mycoplasma hominis and Ureaplasma urealyticum in the genital discharge of symptomatic fertile and asymptomatic infertile women","volume":"33","author":"Casari","year":"2010","journal-title":"New Microbiol."},{"key":"ref_26","first-page":"188","article-title":"Increased Risk of Infertility in Women Infected with Human Papillomavirus","volume":"24","author":"Pebdeni","year":"2023","journal-title":"J. Reprod. Infertil."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"529","DOI":"10.1038\/aja.2013.36","article-title":"Correlation between HPV sperm infection and male infertility","volume":"15","author":"Yang","year":"2013","journal-title":"Asian J. Androl."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"640","DOI":"10.1093\/humrep\/det453","article-title":"Prevalence of human papillomaviruses in semen: A systematic review and meta-analysis","volume":"29","author":"Laprise","year":"2014","journal-title":"Hum. Reprod."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Kaspersen, M.D., Larsen, P.B., Ingerslev, H.J., Fedder, J., Petersen, G.B., Bonde, J., and H\u00f6llsberg, P. (2011). Identification of multiple HPV types on spermatozoa from human sperm donors. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0018095"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Gimenes, F., Medina, F.S., Abreu, A.L.P., Irie, M.M.T., Esqui\u00e7ati, I.B., Malagutti, N., Vasconcellos, V.R.B., Discacciati, M.G., Bonini, M.G., and Maria-Engler, S.S. (2014). Sensitive simultaneous detection of seven sexually transmitted agents in semen by multiplex-PCR and of HPV by single PCR. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0098862"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"dev317","DOI":"10.1093\/humrep\/dev317","article-title":"Presence of human papillomavirus in semen in relation to semen quality","volume":"31","author":"Luttmer","year":"2016","journal-title":"Hum. Reprod."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Foresta, C., Patissini, C., Bertoldo, A., Menegazzo, M., Francavilla, F., Barzon, L., and Ferlin, A. (2011). Mechanism of human papillomavirus binding to human spermatozoa and fertilizing ability of infected spermatozoa. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0015036"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Meccariello, R. (2018). Male accessory glands and sperm function. Spermatozoa-Facts and Perspectives, IntechOpen.","DOI":"10.5772\/68063"},{"key":"ref_34","first-page":"4177","article-title":"Evaluation and treatment of the infertile couple","volume":"83","author":"Forti","year":"1998","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"642","DOI":"10.1111\/bju.12523","article-title":"Varicocele decreases epididymal neutral \u03b1-glucosidase and is associated with alteration of nuclear DNA and plasma membrane in spermatozoa","volume":"113","author":"Camejo","year":"2014","journal-title":"BJU Int."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"955","DOI":"10.1016\/j.fertnstert.2020.01.010","article-title":"Evaluation of human papilloma virus in semen as a risk factor for low sperm quality and poor in vitro fertilization outcomes: A systematic review and meta-analysis","volume":"113","author":"Weinberg","year":"2020","journal-title":"Fertil. Steril."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Capra, G., Notari, T., Butt\u00e0, M., Serra, N., Rizzo, G., and Bosco, L. (2022). Human papillomavirus (HPV) infection and its impact on male infertility. Life, 12.","DOI":"10.3390\/life12111919"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1068","DOI":"10.1067\/mob.2001.115226","article-title":"Human sperm deoxyribonucleic acid fragmentation by specific types of papillomavirus","volume":"184","author":"Connelly","year":"2001","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1006\/gyno.2002.6662","article-title":"Differential effects of human papillomavirus DNA types on p53 tumor-suppressor gene apoptosis in sperm","volume":"85","author":"Lee","year":"2002","journal-title":"Gynecol. Oncol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Damke, E., Kurscheidt, F.A., Balani, V.A., Takeda, K.I., Irie, M.M.T., Gimenes, F., and Consolaro, M.E.L. (2017). Male partners of infertile couples with seminal infections of human papillomavirus have impaired fertility parameters. BioMed. Res. Int., 2017.","DOI":"10.1155\/2017\/4684629"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e28697","DOI":"10.1002\/jmv.28697","article-title":"The impact of human papillomavirus on human reproductive health and the effect on male infertility: An updated review","volume":"95","author":"Das","year":"2023","journal-title":"J. Med. Virol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1016\/j.fertnstert.2012.09.006","article-title":"Association, prevalence, and clearance of human papillomavirus and antisperm antibodies in infected semen samples from infertile patients","volume":"99","author":"Garolla","year":"2013","journal-title":"Fertil. Steril."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.virol.2005.11.025","article-title":"Human papillomaviruses bind a basal extracellular matrix component secreted by keratinocytes which is distinct from a membrane-associated receptor","volume":"347","author":"Culp","year":"2006","journal-title":"Virology"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"709","DOI":"10.1093\/humrep\/dem404","article-title":"Placenta infection with human papillomavirus is associated with spontaneous preterm delivery","volume":"23","author":"Gomez","year":"2008","journal-title":"Hum. Reprod."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3203","DOI":"10.21873\/invivo.12615","article-title":"Presence of HPV 16 and HPV 18 in spermatozoa and embryos of mice","volume":"35","author":"Mastora","year":"2021","journal-title":"In Vivo"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1016\/j.fertnstert.2010.11.047","article-title":"Human papillomavirus infection in couples undergoing in vitro fertilization procedures: Impact on reproductive outcomes","volume":"95","author":"Perino","year":"2011","journal-title":"Fertil. Steril."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1925","DOI":"10.1093\/infdis\/jiaa054","article-title":"Association between maternal human papillomavirus infection and adverse pregnancy outcomes: Systematic review and meta-analysis","volume":"221","author":"Niyibizi","year":"2020","journal-title":"J. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"a1284","DOI":"10.1136\/bmj.a1284","article-title":"Perinatal mortality and other severe adverse pregnancy outcomes associated with treatment of cervical intraepithelial neoplasia: Meta-analysis","volume":"337","author":"Arbyn","year":"2008","journal-title":"BMJ"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1111\/j.1471-0528.2011.02944.x","article-title":"The risk of preterm birth following treatment for precancerous changes in the cervix: A systematic review and meta-analysis","volume":"118","author":"Bruinsma","year":"2011","journal-title":"BJOG"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/jsm.12358","article-title":"Counseling reduces HPVpersistence in coinfected couples","volume":"11","author":"Garolla","year":"2014","journal-title":"J. Sex. Med."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Garbuglia, A.R., Lapa, D., Sias, C., Capobianchi, M.R., and Del Porto, P. (2020). The Use of Both Therapeutic and Prophylactic Vaccines in the Therapy of Papillomavirus Disease. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.00188"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Garolla, A., De Toni, L., Bottacin, A., Valente, U., De Rocco Ponce, M., Di Nisio, A., and Foresta, C. (2018). Human papillomavirus prophylactic vaccination improves reproductive outcome ininfertile patients with HPV semen infection: A retrospective study. Sci. Rep., 8.","DOI":"10.1038\/s41598-018-19369-z"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1080\/13685538.2021.2023126","article-title":"Impact of seminal low-risk human papillomavirus infection on sperm parameters of adult men","volume":"25","author":"Cannarella","year":"2022","journal-title":"Aging Male"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Muscianisi, F., DeToni, L., Giorato, G., Carosso, A., Foresta, C., and Garolla, A. (2021). Is HPV the novel target in male idiopathic infertility? A systematic review of the literature. Front. Endocrinol., 12.","DOI":"10.3389\/fendo.2021.643539"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1093\/humrep\/des009","article-title":"Human papillomavirus sperm infection and assisted reproduction: A dangerous hazard with a possible safe solution","volume":"27","author":"Garolla","year":"2012","journal-title":"Hum. Reprod."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Szab\u00f3, A., V\u00e1ncsa, S., Hegyi, P., V\u00e1radi, A., Forintos, A., Filipov, T., \u00c1cs, J., \u00c1cs, N., Szarvas, T., and Nyir\u00e1dy, P. (2023). Lifestyle-, environmental-, and additional health factors associated with an increased sperm DNA fragmentation: A systematic review and meta-analysis. Reprod. Biol. Endocrinol., 21.","DOI":"10.1186\/s12958-023-01054-0"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/12\/1076\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T16:49:31Z","timestamp":1760114971000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/13\/12\/1076"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,7]]},"references-count":56,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2024,12]]}},"alternative-id":["pathogens13121076"],"URL":"https:\/\/doi.org\/10.3390\/pathogens13121076","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,12,7]]}}}