{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T03:18:48Z","timestamp":1792120728605,"version":"4.4.0"},"reference-count":74,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2024,12,27]],"date-time":"2024-12-27T00:00:00Z","timestamp":1735257600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministry of Health","doi-asserted-by":"publisher","award":["NU23-05-00511"],"award-info":[{"award-number":["NU23-05-00511"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003243","name":"Ministry of Health","doi-asserted-by":"publisher","award":["284423"],"award-info":[{"award-number":["284423"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007543","name":"Charles University Grant Agency","doi-asserted-by":"publisher","award":["NU23-05-00511"],"award-info":[{"award-number":["NU23-05-00511"]}],"id":[{"id":"10.13039\/100007543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007543","name":"Charles University Grant Agency","doi-asserted-by":"publisher","award":["284423"],"award-info":[{"award-number":["284423"]}],"id":[{"id":"10.13039\/100007543","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Bartonellae are zoonotic pathogens with a broad range of reservoir hosts and vectors. To examine sylvatic Bartonella reservoirs, tissue samples of red deer (Cervus elaphus, n = 114) and their associated deer keds (Lipoptena cervi, n = 50; L. fortisetosa, n = 272) collected in the Czech Republic were tested for the presence of Bartonella using PCR at four loci (gltA, rpoB, nuoG, ITS); PCR sensitivity was increased significantly by using primers modified for the detection of wildlife-associated bartonellae. One-third of the deer and 70% of the deer keds were Bartonella positive; within the tested animal tissues, usually the spleen was positive. The most prevalent Bartonella represents an undescribed species related to isolates from Japanese sika deer and L. fortisetosa. Additionally, B. schoenbuchensis sensu lato and B. bovis were found, together making up 17 genotypes characterized by multi-locus sequence typing, all unique compared to previously published sequences. Nanopore sequencing of selected samples revealed an additional 14 unique Bartonella genotypes, with up to six genotypes co-infecting one deer, highlighting the diversity of ruminant Bartonella. The high COI variety of examined L. cervi and L. fortisetosa suggests L. fortisetosa in central Europe is not a homogenous invasive population.<\/jats:p>","DOI":"10.3390\/pathogens14010006","type":"journal-article","created":{"date-parts":[[2024,12,31]],"date-time":"2024-12-31T14:21:12Z","timestamp":1735654872000},"page":"6","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Diversity and Multiple Infections of Bartonella in Red Deer and Deer Keds"],"prefix":"10.3390","volume":"14","author":[{"given":"Iva","family":"Hammerbauerov\u00e1","sequence":"first","affiliation":[{"name":"Department of Parasitology, Faculty of Science, Charles University, 128 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Richtrov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health, \u0160rob\u00e1rova 49\/48, 100 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate\u0159ina","family":"Kybicov\u00e1","sequence":"additional","affiliation":[{"name":"National Institute of Public Health, \u0160rob\u00e1rova 49\/48, 100 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8706-9371","authenticated-orcid":false,"given":"Petr","family":"Pajer","sequence":"additional","affiliation":[{"name":"Military Health Institute, Military Medical Agency, Tychonova 1, 160 01 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0552-9363","authenticated-orcid":false,"given":"Jan","family":"Vot\u00fdpka","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Faculty of Science, Charles University, 128 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,12,27]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/S0891-5520(05)70414-1","article-title":"Bartonella-associated Infections","volume":"12","author":"Spach","year":"1998","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1001\/jama.1994.03510310061039","article-title":"Rochalimaea henselae Infection: A New Zoonosis With the Domestic Cat as Reservoir","volume":"271","author":"Koehler","year":"1994","journal-title":"JAMA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"713","DOI":"10.4269\/ajtmh.1969.18.713","article-title":"Trench Fever. III. Induction of Clinical Disease in Volunteers Inoculated with Rickettsia quintana Propagated on Blood Agar","volume":"18","author":"Vinson","year":"1969","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"872","DOI":"10.1128\/jcm.31.4.872-881.1993","article-title":"Rochalimaea elizabethae sp. Nov. Isolated from a Patient with Endocarditis","volume":"31","author":"Daly","year":"1993","journal-title":"J. Clin. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1813","DOI":"10.1128\/jcm.35.7.1813-1818.1997","article-title":"Bartonella clarridgeiae, a Newly Recognized Zoonotic Pathogen Causing Inoculation Papules, Fever, and Lymphadenopathy (Cat Scratch Disease)","volume":"35","author":"Kordick","year":"1997","journal-title":"J. Clin. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"4034","DOI":"10.1128\/JCM.37.12.4034-4038.1999","article-title":"Demonstration of Bartonella grahamii DNA in Ocular Fluids of a Patient with Neuroretinitis","volume":"37","author":"Kerkhoff","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1128\/JCM.38.4.1698-1700.2000","article-title":"Bartonella vinsonii Subsp. Berkhoffii as an Agent of Afebrile Blood Culture-Negative Endocarditis in a Human","volume":"38","author":"Roux","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"645","DOI":"10.1128\/JCM.41.2.645-650.2003","article-title":"Bartonella Strains from Ground Squirrels Are Identical to Bartonella washoensis Isolated from a Human Patient","volume":"41","author":"Kosoy","year":"2003","journal-title":"J. Clin. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"3462","DOI":"10.1128\/JCM.42.8.3462-3468.2004","article-title":"Bartonella koehlerae, a New Cat-Associated Agent of Culture-Negative Human Endocarditis","volume":"42","author":"Avidor","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1128\/JCM.43.2.945-947.2005","article-title":"Bartonella vinsonii Subsp. Arupensis as an Agent of Blood Culture-Negative Endocarditis in a Human","volume":"43","author":"Fenollar","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1128\/JCM.44.1.278-279.2006","article-title":"First Isolation of Bartonella alsatica from a Valve of a Patient with Endocarditis","volume":"44","author":"Raoult","year":"2006","journal-title":"J. Clin. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"2381","DOI":"10.1056\/NEJMoa065987","article-title":"Bacteremia, Fever, and Splenomegaly Caused by a Newly Recognized Bartonella Species","volume":"356","author":"Eremeeva","year":"2007","journal-title":"N. Engl. J. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1128\/JCM.02120-07","article-title":"Bartonella tamiae sp. Nov., a Newly Recognized Pathogen Isolated from Three Human Patients from Thailand","volume":"46","author":"Kosoy","year":"2007","journal-title":"J. Clin. Microbiol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"66","DOI":"10.3201\/eid1501.081080","article-title":"Isolation of Candidatus Bartonella melophagi from Human Blood","volume":"15","author":"Maggi","year":"2009","journal-title":"Emerg. Infect. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1111\/j.1469-0691.2008.02197.x","article-title":"Serological Evidence of Bartonella vinsonii Lymphadenopathies in a Child Bitten by a Dog","volume":"15","author":"Rolain","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1140","DOI":"10.4269\/ajtmh.2010.09-0778","article-title":"Identification of Bartonella Infections in Febrile Human Patients from Thailand and Their Potential Animal Reservoirs","volume":"82","author":"Kosoy","year":"2010","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"500","DOI":"10.3201\/eid1603.081673","article-title":"Candidatus Bartonella mayotimonensis and Endocarditis","volume":"16","author":"Lin","year":"2010","journal-title":"Emerg. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3865","DOI":"10.1128\/JCM.01226-13","article-title":"Molecular Typing of \u201cCandidatus Bartonella ancashi\u201d, a New Human Pathogen Causing Verruga Peruana","volume":"51","author":"Mullins","year":"2013","journal-title":"J. Clin. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"544","DOI":"10.3201\/eid2203.151823","article-title":"Human Lymphadenopathy Caused by Ratborne Bartonella, Tbilisi, Georgia","volume":"22","author":"Kandelaki","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"457","DOI":"10.3201\/eid2203.150269","article-title":"Identification of Novel Zoonotic Activity of Bartonella spp., France","volume":"22","author":"Moutailler","year":"2016","journal-title":"Emerg. Infect. Dis."},{"key":"ref_21","unstructured":"Czech Statistical Office (2023, November 27). Basic Data on Hunting Grounds, Game Stock. and Hunting\u2014From 1 April 2022 to 31 March 2023. Available online: https:\/\/www.czso.cz\/csu\/czso\/basic-data-on-hunting-grounds-game-stock-and-hunting-from-1-april-2022-to-31-march-2023."},{"key":"ref_22","unstructured":"Lotock\u00fd, M., and Turek, K. (2023). Mysliveck\u00e1 statistika 2022\/2023. Myslivost, 2023."},{"key":"ref_23","unstructured":"Czech Statistical Office (2023, October 30). Population\u2014Annual Time Series. Available online: https:\/\/www.czso.cz\/csu\/czso\/population_hd."},{"key":"ref_24","unstructured":"Oliva, F. (2024, June 24). OT\u00c1ZKY NA V\u00cdKEND: \u010ce\u0161i Potvrdili Pov\u011bst Houba\u0159sk\u00e9ho N\u00e1roda. Sb\u00edr\u00e1me i Lesn\u00ed Plody. SC&C. Available online: https:\/\/scac.cz\/otazky-na-vikend-cesi-potvrdili-povest-houbarskeho-naroda-sbirame-i-lesni-plody\/."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Engel, P., Salzburger, W., Liesch, M., Chang, C.-C., Maruyama, S., Lanz, C., Calteau, A., Lajus, A., M\u00e9digue, C., and Schuster, S.C. (2011). Parallel Evolution of a Type IV Secretion System in Radiating Lineages of the Host-Restricted Bacterial Pathogen Bartonella. PLoS Genet., 7.","DOI":"10.1371\/journal.pgen.1001296"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"5607","DOI":"10.1099\/ijsem.0.004332","article-title":"List of Prokaryotic Names with Standing in Nomenclature (LPSN) Moves to the DSMZ","volume":"70","author":"Parte","year":"2020","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1016\/j.jiph.2024.02.009","article-title":"Geographical Distribution of Bartonella spp. in the Countries of the WHO Eastern Mediterranean Region (WHO-EMRO)","volume":"17","author":"Ahmadinezhad","year":"2024","journal-title":"J. Infect. Public Health"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Amaral, R.B.D., Cardozo, M.V., Varani, A.d.M., Furquim, M.E.C., Dias, C.M., de Assis, W.O., da Silva, A.R., Herrera, H.M., Machado, R.Z., and Andr\u00e9, M.R. (2022). First Report of Bartonella spp. in Marsupials from Brazil, with a Description of Bartonella harrusi sp. Nov. and a New Proposal for the Taxonomic Reclassification of Species of the Genus Bartonella. Microorganisms, 10.","DOI":"10.3390\/microorganisms10081609"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Miao, Y., Guo, W., Zhang, W., Chen, Z., Mian, D., Li, R., Xu, A., Chen, M., and Li, D. (2024). Detection of Bartonella spp. in Farmed Deer (Artiodactyla: Cervidae) Using Multiplex Assays in the Qinghai-Tibet Plateau, China. Microbiol. Spectr., 12.","DOI":"10.1128\/spectrum.04120-23"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e228","DOI":"10.1111\/tbed.12335","article-title":"Molecular Survey of Anaplasma and Ehrlichia of Red Deer and Sika Deer in Gansu, China in 2013","volume":"63","author":"Li","year":"2016","journal-title":"Transbound. Emerg. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1557","DOI":"10.1099\/00207713-51-4-1557","article-title":"Bartonella schoenbuchii sp. Nov., Isolated from the Blood of Wild Roe Deer","volume":"51","author":"Dehio","year":"2001","journal-title":"Int. J. Syst. Evol. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1017\/S0950268814001411","article-title":"Molecular Detection of Bartonella spp. in Deer Ked Pupae, Adult Keds and Moose Blood in Finland","volume":"143","author":"Korhonen","year":"2015","journal-title":"Epidemiol. Infect."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"316","DOI":"10.1111\/zph.12827","article-title":"Zoonotic Bartonella Species in Eurasian Wolves and Other Free-Ranging Wild Mammals from Italy","volume":"68","author":"Greco","year":"2021","journal-title":"Zoonoses Public Health"},{"key":"ref_34","first-page":"135","article-title":"Lausfliegen-Pr\u00e4parate aus Instituts- und Museumssammlungen Sachsens","volume":"63","author":"Jentzsch","year":"2019","journal-title":"Entomol. Nachrichten Berichte"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"307","DOI":"10.2340\/0001555562307311","article-title":"Persistent Pruritic Papules from Deer Ked Bites","volume":"62","author":"Rantanen","year":"1982","journal-title":"Acta Derm. Venereol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Buczek, W., Buczek, A.M., Bartosik, K., and Buczek, A. (2020). Comparison of Skin Lesions Caused by Ixodes ricinus Ticks and Lipoptena cervi Deer Keds Infesting Humans in the Natural Environment. Int. J. Environ. Res. Public Health, 17.","DOI":"10.3390\/ijerph17093316"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5320","DOI":"10.1128\/JCM.42.11.5320-5323.2004","article-title":"Isolation of Bartonella schoenbuchensis from Lipoptena cervi, a Blood-Sucking Arthropod Causing Deer Ked Dermatitis","volume":"42","author":"Dehio","year":"2004","journal-title":"J. Clin. Microbiol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Ma\u015blanko, W., Bartosik, K., Raszewska-Famielec, M., Szwaj, E., and Asman, M. (2020). Exposure of Humans to Attacks by Deer Keds and Consequences of Their Bites\u2014A Case Report with Environmental Background. Insects, 11.","DOI":"10.3390\/insects11120859"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1186\/s13071-015-0764-y","article-title":"Vertical Transmission of Bartonella schoenbuchensis in Lipoptena cervi","volume":"8","author":"Jahfari","year":"2015","journal-title":"Parasites Vectors"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1128\/AEM.02632-12","article-title":"Bartonella Infections in Deer Keds (Lipoptena cervi) and Moose (Alces alces) in Norway","volume":"79","author":"Duodu","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6302","DOI":"10.1128\/AEM.70.10.6302-6305.2004","article-title":"Role of Hippoboscidae Flies as Potential Vectors of Bartonella spp. Infecting Wild and Domestic Ruminants","volume":"70","author":"Halos","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Regier, Y., Komma, K., Weigel, M., Pulliainen, A.T., G\u00f6ttig, S., Hain, T., and Kempf, V.A.J. (2018). Microbiome Analysis Reveals the Presence of Bartonella spp. and Acinetobacter spp. in Deer Keds (Lipoptena cervi). Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.03100"},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Szewczyk, T., Werszko, J., Steiner-Bogdaszewska, \u017b., Je\u017cewski, W., Laskowski, Z., and Karbowiak, G. (2017). Molecular Detection of Bartonella spp. in Deer Ked (Lipoptena cervi) in Poland. Parasites Vectors, 10.","DOI":"10.1186\/s13071-017-2413-0"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Vogt, I., Schr\u00f6ter, S., Schreiter, R., Sprong, H., Volfov\u00e1, K., Jentzsch, M., and Freick, M. (2024). Detection of Bartonella schoenbuchensis (Sub)Species DNA in Different Louse Fly Species in Saxony, Germany: The Proof of Multiple PCR Analysis Necessity in Case of Ruminant-Associated Bartonellae Determination. Vet. Med. Sci., 10.","DOI":"10.1002\/vms3.1417"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Bartosik, K., Ma\u015blanko, W., Buczek, A., Asman, M., Witecka, J., Szwaj, E., B\u0142aszkiewicz, P.S., and \u015awis\u0142ocka, M. (2021). Two New Haplotypes of Bartonella sp. Isolated from Lipoptena fortisetosa (Diptera: Hippoboscidae) in SE Poland. Insects, 12.","DOI":"10.3390\/insects12060485"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Ga\u0142\u0119cki, R., Jaroszewski, J., Baku\u0142a, T., Galon, E.M., and Xuan, X. (2021). Molecular Detection of Selected Pathogens with Zoonotic Potential in Deer Keds (Lipoptena fortisetosa). Pathogens, 10.","DOI":"10.3390\/pathogens10030324"},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Sato, S., Kabeya, H., Ishiguro, S., Shibasaki, Y., and Maruyama, S. (2021). Lipoptena fortisetosa as a Vector of Bartonella Bacteria in Japanese Sika Deer (Cervus nippon). Parasites Vectors, 14.","DOI":"10.1186\/s13071-021-04585-w"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Solis Cayo, L., Hammerbauerov\u00e1, I., Sommer, J., Nemati, Z., Ballhorn, W., Tsukayama, P., Dichter, A., Vot\u00fdpka, J., and Kempf, V.A.J. (2024). Genome Sequences of Three Bartonella schoenbuchensis Strains from Czechia. Microbiol. Resour. Announc., 13.","DOI":"10.1128\/mra.00397-24"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1089\/vbz.2016.1956","article-title":"Guidelines for the Isolation, Molecular Detection, and Characterization of Bartonella Species","volume":"17","author":"Buffet","year":"2017","journal-title":"Vector Borne Zoonotic Dis."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"5224","DOI":"10.1128\/AEM.00621-08","article-title":"Analysis of a Novel Insect Cell Culture Medium-Based Growth Medium for Bartonella Species","volume":"74","author":"Riess","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_51","unstructured":"Larsson, J. (2024, January 16). Eulerr: Area-Proportional Euler and Venn Diagrams with Ellipses. Available online: https:\/\/CRAN.R-project.org\/package=eulerr."},{"key":"ref_52","unstructured":"Larsson, J. (2018). Eulerr: Area-Proportional Euler Diagrams with Ellipses. [Bachelor\u2019s Thesis, Lund University]."},{"key":"ref_53","first-page":"294","article-title":"DNA Primers for Amplification of Mitochondrial Cytochrome c Oxidase Subunit I from Diverse Metazoan Invertebrates","volume":"3","author":"Folmer","year":"1994","journal-title":"Mol. Mar. Biol. Biotechnol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1797","DOI":"10.1128\/jcm.33.7.1797-1803.1995","article-title":"Differentiation of Bartonella-like Isolates at the Species Level by PCR-Restriction Fragment Length Polymorphism in the Citrate Synthase Gene","volume":"33","author":"Norman","year":"1995","journal-title":"J. Clin. Microbiol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1099\/00207713-46-4-891","article-title":"Comparison of Partial Citrate Synthase Gene (gltA) Sequences for Phylogenetic Analysis of Bartonella Species","volume":"46","author":"Birtles","year":"1996","journal-title":"Int. J. Syst. Bacteriol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"7850","DOI":"10.1128\/AEM.06012-11","article-title":"Molecular Detection and Identification of Bartonella Species in Xenopsylla cheopis Fleas (Siphonaptera: Pulicidae) Collected from Rattus norvegicus Rats in Los Angeles, California","volume":"77","author":"Billeter","year":"2011","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1128\/JCM.43.3.1171-1176.2005","article-title":"Potential Limitations of the 16S-23S rRNA Intergenic Region for Molecular Detection of Bartonella Species","volume":"43","author":"Maggi","year":"2005","journal-title":"J. Clin. Microbiol."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"4630","DOI":"10.1128\/JCM.00470-10","article-title":"Improved Detection of Bartonella DNA in Mammalian Hosts and Arthropod Vectors by Real-Time PCR Using the NADH Dehydrogenase Gamma Subunit (nuoG)","volume":"48","author":"Colborn","year":"2010","journal-title":"J. Clin. Microbiol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1128\/JCM.39.2.430-437.2001","article-title":"Use of rpoB Gene Analysis for Detection and Identification of Bartonella Species","volume":"39","author":"Renesto","year":"2001","journal-title":"J. Clin. Microbiol."},{"key":"ref_60","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., and Holt, K.E. (2019). Performance of Neural Network Basecalling Tools for Oxford Nanopore Sequencing. Genome Biol., 20.","DOI":"10.1186\/s13059-019-1727-y"},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Wick, R.R., Judd, L.M., Gorrie, C.L., and Holt, K.E. (2017). Completing Bacterial Genome Assemblies with Multiplex MinION Sequencing. Microb. Genom., 3.","DOI":"10.1099\/mgen.0.000132"},{"key":"ref_62","unstructured":"Biomatters (2024, February 06). Geneious Prime 2023.2.1. Available online: https:\/\/www.geneious.com."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3059","DOI":"10.1093\/nar\/gkf436","article-title":"MAFFT: A Novel Method for Rapid Multiple Sequence Alignment Based on Fast Fourier Transform","volume":"30","author":"Katoh","year":"2002","journal-title":"Nucleic Acids Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1093\/molbev\/mst010","article-title":"MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability","volume":"30","author":"Katoh","year":"2013","journal-title":"Mol. Biol. Evol."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"754","DOI":"10.1093\/bioinformatics\/17.8.754","article-title":"MRBAYES: Bayesian Inference of Phylogenetic Trees","volume":"17","author":"Huelsenbeck","year":"2001","journal-title":"Bioinformatics"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1093\/sysbio\/syq010","article-title":"New Algorithms and Methods to Estimate Maximum-Likelihood Phylogenies: Assessing the Performance of PhyML 3.0","volume":"59","author":"Guindon","year":"2010","journal-title":"Syst. Biol."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1093\/oxfordjournals.molbev.a026036","article-title":"Median-Joining Networks for Inferring Intraspecific Phylogenies","volume":"16","author":"Bandelt","year":"1999","journal-title":"Mol. Biol. Evol."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"1110","DOI":"10.1111\/2041-210X.12410","article-title":"POPART: Full-Feature Software for Haplotype Network Construction","volume":"6","author":"Leigh","year":"2015","journal-title":"Methods Ecol. Evol."},{"key":"ref_69","first-page":"275","article-title":"Lipoptena parvula, n. sp., Eine Neue Art Aus Der Tschechoslowakei (Diptera, Hippoboscidae)","volume":"1967","author":"Theodor","year":"1967","journal-title":"Acta Entomol. Musei Natl. Pragae"},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1625","DOI":"10.1056\/NEJM199212033272303","article-title":"Isolation of Rochalimaea Species from Cutaneous and Osseous Lesions of Bacillary Angiomatosis","volume":"327","author":"Koehler","year":"1992","journal-title":"N. Engl. J. Med."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1016\/j.cimid.2012.07.001","article-title":"Prevalence and Genetic Diversity of Bartonella Species in Sika Deer (Cervus nippon) in Japan","volume":"35","author":"Sato","year":"2012","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1644\/11-MAMM-A-022.1","article-title":"Hybridization between Native and Introduced Species of Deer in Eastern Europe","volume":"93","author":"Biedrzycka","year":"2012","journal-title":"J. Mammal."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.cimid.2018.06.003","article-title":"Infections with Bartonella spp. in Free-Ranging Cervids and Deer Keds (Lipoptena cervi) in Norway","volume":"58","author":"Razanske","year":"2018","journal-title":"Comp. Immunol. Microbiol. Infect. Dis."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"505","DOI":"10.7589\/2019-08-196","article-title":"Diverse Bartonella spp. Detected in White-Tailed Deer (Odocoileus virginianus) and Associated Keds (Lipoptena mazamae) in the Southeastern USA","volume":"56","author":"Izenour","year":"2020","journal-title":"J. Wildl. 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