{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T19:03:03Z","timestamp":1774465383710,"version":"3.50.1"},"reference-count":58,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T00:00:00Z","timestamp":1736985600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Medical Biological Defense Research Program of the Bundeswehr Joint Medical Service"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The anthrax pathogen Bacillus anthracis can remain dormant as spores in soil for many years. This applies to both natural foci and to sites of anthropogenic activity such as tanneries, abattoirs, or wool factories. The A.Br.075 (A-branch) clade (also known as A.Br.Sterne) is prominent not only because it comprises several outbreak strains but even more so because spore preparations of its namesake, the Sterne strain, are counted among the most utilized anthrax animal vaccines. In this study, we genome-sequenced and analyzed 56 additional B. anthracis isolates of the A.Br.075 clade. Four of these we recently retrieved from soil samples taken from a decades-long abandoned tannery. The other 52 strains originated from our archival collection from the 20th century. Notably, the extended phylogeny of the A.Br.075 clade indicated that many of the newly added chromosomes represent basal members, some of which are among the most basal strains from this lineage. Twelve new strains populate a very deep-branching lineage we have named A.Br.Ortho-Sterne (also known as A.Br.076). A further 11 isolates amend the clade named A.Br.Para-Sterne (A.Br.078). Finally, some of the terminal clusters of the clade named A.Br.Eu-Sterne appear to be replete with (near) identical isolates, possibly a result of widespread use of the Sterne vaccine and of its re-isolation from vaccination-related animal anthrax outbreaks. From the accrued new phylogenetic information, we designed and tested a variety of new SNP-PCR assays for rapid and facile genotyping of unassigned B. anthracis genomes. Lastly, the successful isolation of live B. anthracis from a long-abandoned tannery reemphasizes the need for continued risk awareness of such sites.<\/jats:p>","DOI":"10.3390\/pathogens14010083","type":"journal-article","created":{"date-parts":[[2025,1,16]],"date-time":"2025-01-16T10:44:23Z","timestamp":1737024263000},"page":"83","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Archival and Newly Isolated Historical Bacillus anthracis Strains Populate the Deeper Phylogeny of the A.Br.075(Sterne) Clade"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4013-8808","authenticated-orcid":false,"given":"Markus","family":"Antwerpen","sequence":"first","affiliation":[{"name":"Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Braun","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany"},{"name":"Institute of Infectious Diseases and Tropical Medicine, University Hospital Ludwig-Maximilian University Munich, 80799 Munich, Germany"},{"name":"Fraunhofer Institute for Translational Medicine and Pharmacology (ITMP), Immunology, Infection and Pandemic Research, 80799 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wolfgang","family":"Beyer","sequence":"additional","affiliation":[{"name":"Department of Livestock Infectiology and Environmental Hygiene, Institute of Animal Science, University of Hohenheim, 70599 Stuttgart, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk","family":"Aldenkortt","sequence":"additional","affiliation":[{"name":"Enviro Services International Sarl, Livange, 3378 Luxembourg, Luxembourg"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Seidel","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8002-7718","authenticated-orcid":false,"given":"Gregor","family":"Grass","sequence":"additional","affiliation":[{"name":"Bundeswehr Institute of Microbiology (IMB), 80937 Munich, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,16]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"\u00c5gren, J., Finn, M., Bengtsson, B., and Segerman, B. (2014). Microevolution during an Anthrax Outbreak Leading to Clonal Heterogeneity and Penicillin Resistance. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0089112"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Braun, P., Beyer, W., Hanczaruk, M., Riehm, J.M., Antwerpen, M., Otterbein, C., Oesterheld, J., and Grass, G. (2022). Reoccurring Bovine Anthrax in Germany on the Same Pasture after 12 Years. J. Clin. Microbiol., 60.","DOI":"10.1128\/jcm.02291-21"},{"key":"ref_3","unstructured":"Turnbull, P.C. (2008). World Health Organization. Anthrax in Humans and Animals, WHO Press."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"174","DOI":"10.1007\/s10393-020-01474-z","article-title":"Factors Contributing to Anthrax Outbreaks in the Circumpolar North","volume":"17","author":"Hueffer","year":"2020","journal-title":"Ecohealth"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Timofeev, V., Bahtejeva, I., Mironova, R., Titareva, G., Lev, I., Christiany, D., Borzilov, A., Bogun, A., and Vergnaud, G. (2019). Insights from Bacillus anthracis Strains Isolated from Permafrost in the Tundra Zone of Russia. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0209140"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"254","DOI":"10.1038\/294254a0","article-title":"Bacillus anthracis on Gruinard Island","volume":"294","author":"Manchee","year":"1981","journal-title":"Nature"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"356","DOI":"10.1016\/j.mam.2009.08.003","article-title":"The Ecology of Bacillus anthracis","volume":"30","author":"Blackburn","year":"2009","journal-title":"Mol. Asp. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/S0944-5013(11)80054-6","article-title":"A Nested PCR Method for the Detection of Bacillus anthracis in Environmental Samples Collected from Former Tannery Sites","volume":"150","author":"Beyer","year":"1995","journal-title":"Microbiol. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"50","DOI":"10.1016\/j.meegid.2016.10.019","article-title":"Comparative Genomics of Bacillus anthracis from the Wool Industry Highlights Polymorphisms of Lineage A.Br.Vollum","volume":"46","author":"Derzelle","year":"2016","journal-title":"Infect. Genet. Evol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"4005","DOI":"10.1128\/AEM.00417-08","article-title":"Occurrence and Genetic Diversity of Bacillus anthracis Strains Isolated in an Active Wool-Cleaning Factory","volume":"74","author":"Wattiau","year":"2008","journal-title":"Appl. Env. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1046\/j.1365-2672.1999.00867.x","article-title":"1996\u201397 Global Anthrax Report","volume":"87","year":"1999","journal-title":"J. Appl. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1046\/j.1469-0691.2002.00500.x","article-title":"Anthrax in Europe: Its Epidemiology, Clinical Characteristics, and Role in Bioterrorism","volume":"8","author":"Schmid","year":"2002","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Girault, G., Blouin, Y., Vergnaud, G., and Derzelle, S. (2014). High-Throughput Sequencing of Bacillus anthracis in France: Investigating Genome Diversity and Population Structure Using Whole-Genome SNP Discovery. BMC Genom., 15.","DOI":"10.1186\/1471-2164-15-288"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Vergnaud, G., Girault, G., Thierry, S., Pourcel, C., Madani, N., and Blouin, Y. (2016). Comparison of French and Worldwide Bacillus anthracis Strains Favors a Recent, Post-Columbian Origin of the Predominant North-American Clade. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0146216"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Antwerpen, M., Beyer, W., Bassy, O., Ortega-Garc\u00eda, M.V., Cabria-Ramos, J.C., Grass, G., and W\u00f6lfel, R. (2019). Phylogenetic Placement of Isolates within the Trans-Eurasian Clade A.Br.008\/009 of Bacillus anthracis. Microorganisms, 7.","DOI":"10.3390\/microorganisms7120689"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Braun, P., Knupfer, M., Antwerpen, M., Triebel, D., and Grass, G. (2020). A Rare Glimpse into the Past of the Anthrax Pathogen Bacillus anthracis. Microorganisms, 8.","DOI":"10.3390\/microorganisms8020298"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1016\/j.meegid.2015.03.030","article-title":"Molecular Diversity of Bacillus anthracis in the Netherlands: Investigating the Relationship to the Worldwide Population Using Whole-Genome SNP Discovery","volume":"32","author":"Derzelle","year":"2015","journal-title":"Infect. Genet. Evol."},{"key":"ref_18","first-page":"16","article-title":"Anthrax","volume":"Volume 1","author":"Stableforth","year":"1959","journal-title":"Infectious Diseases of Animals, Disease Due to Bacteria"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Beyer, W., Bellan, S., Eberle, G., Ganz, H.H., Getz, W.M., Haumacher, R., Hilss, K.A., Kilian, W., Lazak, J., and Turner, W.C. (2012). Distribution and Molecular Evolution of Bacillus anthracis Genotypes in Namibia. PLoS Negl. Trop. Dis., 6.","DOI":"10.1371\/journal.pntd.0001534"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Lekota, K.E., Hassim, A., Ledwaba, M.B., Glover, B.A., Dekker, E.H., Van Schalkwyk, L.O., Rossouw, J., Beyer, W., Vergnaud, G., and Van Heerden, H. (2024). Bacillus anthracis in South Africa, 1975\u20132013: Are Some Lineages Vanishing?. BMC Genom., 25.","DOI":"10.1186\/s12864-024-10631-5"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e01501-16","DOI":"10.1128\/mBio.01501-16","article-title":"A Bacillus anthracis Genome Sequence from the Sverdlovsk 1979 Autopsy Specimens","volume":"7","author":"Sahl","year":"2016","journal-title":"MBio"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Van Ert, M.N., Easterday, W.R., Huynh, L.Y., Okinaka, R.T., Hugh-Jones, M.E., Ravel, J., Zanecki, S.R., Pearson, T., Simonson, T.S., and U\u2019Ren, J.M. (2007). Global Genetic Population Structure of Bacillus anthracis. PLoS ONE, 2.","DOI":"10.1371\/journal.pone.0000461"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Derzelle, S., Girault, G., Kokotovic, B., and Angen, O. (2015). Whole Genome-Sequencing and Phylogenetic Analysis of a Historical Collection of Bacillus anthracis Strains from Danish Cattle. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0134699"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.meegid.2019.03.022","article-title":"Comparative Genomics and Phylogenetic Analysis of Bacillus anthracis Strains Isolated from Domestic Animals in Japan","volume":"71","author":"Okutani","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.meegid.2016.08.017","article-title":"Unexpected Genomic Relationships between Bacillus anthracis Strains from Bangladesh and Central Europe","volume":"45","author":"Rume","year":"2016","journal-title":"Infect. Genet. Evol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"104146","DOI":"10.1016\/j.meegid.2019.104146","article-title":"Phylogenomic Structure of Bacillus anthracis Isolates in the Northern Cape Province, South Africa Revealed Novel Single Nucleotide Polymorphisms","volume":"80","author":"Lekota","year":"2020","journal-title":"Infect. Genet. Evol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Fasanella, A., Di Taranto, P., Garofolo, G., Colao, V., Marino, L., Buonavoglia, D., Pedarra, C., Adone, R., and Hugh-Jones, M. (2013). Ground Anthrax Bacillus Refined Isolation (GABRI) Method for Analyzing Environmental Samples with Low Levels of Bacillus anthracis Contamination. BMC Microbiol., 13.","DOI":"10.1186\/1471-2180-13-167"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Braun, P., Wolfschl\u00e4ger, I., Reetz, L., Bachstein, L., Jacinto, A.C., Tocantins, C., Poppe, J., and Grass, G. (2020). Rapid Microscopic Detection of Bacillus anthracis by Fluorescent Receptor Binding Proteins of Bacteriophages. Microorganisms, 8.","DOI":"10.3390\/microorganisms8060934"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/j.mcp.2008.06.001","article-title":"Real-Time PCR System Targeting a Chromosomal Marker Specific for Bacillus anthracis","volume":"22","author":"Antwerpen","year":"2008","journal-title":"Mol. Cell Probes"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Birdsell, D.N., Pearson, T., Price, E.P., Hornstra, H.M., Nera, R.D., Stone, N., Gruendike, J., Kaufman, E.L., Pettus, A.H., and Hurbon, A.N. (2012). Melt Analysis of Mismatch Amplification Mutation Assays (Melt-MAMA): A Functional Study of a Cost-Effective SNP Genotyping Assay in Bacterial Models. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0032866"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Antwerpen, M., Beyer, W., and Grass, G. (2024). New Insights into the Phylogeny of the A.Br.161 (\u201cA.Br.Heroin\u201d) Clade of Bacillus anthracis. Pathogens, 13.","DOI":"10.3390\/pathogens13070593"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Braun, P., Grass, G., Aceti, A., Serrecchia, L., Affuso, A., Marino, L., Grimaldi, S., Pagano, S., Hanczaruk, M., and Georgi, E. (2015). Microevolution of Anthrax from a Young Ancestor (M.A.Y.A.) Suggests a Soil-Borne Life Cycle of Bacillus anthracis. PLoS ONE, 10.","DOI":"10.1371\/journal.pone.0135346"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1089\/cmb.2012.0021","article-title":"SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing","volume":"19","author":"Bankevich","year":"2012","journal-title":"J. Comput. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"Walker, B.J., Abeel, T., Shea, T., Priest, M., Abouelliel, A., Sakthikumar, S., Cuomo, C.A., Zeng, Q., Wortman, J., and Young, S.K. (2014). Pilon: An Integrated Tool for Comprehensive Microbial Variant Detection and Genome Assembly Improvement. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0112963"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Treangen, T.J., Ondov, B.D., Koren, S., and Phillippy, A.M. (2014). The Harvest Suite for Rapid Core-Genome Alignment and Visualization of Thousands of Intraspecific Microbial Genomes. Genome Biol., 15.","DOI":"10.1186\/s13059-014-0524-x"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1111\/j.1096-0031.1999.tb00277.x","article-title":"The Parsimony Ratchet, a New Method for Rapid Parsimony Analysis","volume":"15","author":"Nixon","year":"1999","journal-title":"Cladistics Int. J. Willi Hennig Soc."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1093\/bioinformatics\/btq706","article-title":"Phangorn: Phylogenetic Analysis in R","volume":"27","author":"Schliep","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1395","DOI":"10.1101\/gr.232397.117","article-title":"GrapeTree: Visualization of Core Genomic Relationships among 100,000 Bacterial Pathogens","volume":"28","author":"Zhou","year":"2018","journal-title":"Genome Res."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Simonson, T.S., Okinaka, R.T., Wang, B., Easterday, W.R., Huynh, L., U\u2019Ren, J.M., Dukerich, M., Zanecki, S.R., Kenefic, L.J., and Beaudry, J. (2009). Bacillus anthracis in China and Its Relationship to Worldwide Lineages. BMC Microbiol., 9.","DOI":"10.1186\/1471-2180-9-71"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"105496","DOI":"10.1016\/j.meegid.2023.105496","article-title":"Spatial and Phylogenetic Patterns Reveal Hidden Infection Sources of Bacillus anthracis in an Anthrax Outbreak in Son La Province, Vietnam","volume":"114","author":"Metrailer","year":"2023","journal-title":"Infect. Genet. Evol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.3201\/eid1409.080076","article-title":"Texas Isolates Closely Related to Bacillus anthracis Ames","volume":"14","author":"Kenefic","year":"2008","journal-title":"Emerg. Infect. Dis."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"S166","DOI":"10.1089\/bsp.2013.0003","article-title":"Genetic Diversity of Bacillus anthracis in Europe: Genotyping Methods in Forensic and Epidemiologic Investigations","volume":"11","author":"Derzelle","year":"2013","journal-title":"Biosecur. Bioterror."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"104890","DOI":"10.1016\/j.meegid.2021.104890","article-title":"Phylogenetics of Bacillus anthracis Isolates from Russia and Bordering Countries","volume":"92","author":"Eremenko","year":"2021","journal-title":"Infect. Genet. Evol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Wang, D., Wang, B., Zhu, L., Wu, S., Lyu, Y., Feng, E., Pan, C., Jiao, L., Cui, Y., and Liu, X. (2020). Genotyping and Population Diversity of Bacillus anthracis in China Based on MLVA and canSNP Analysis. Microbiol. Res., 233.","DOI":"10.1016\/j.micres.2020.126414"},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Zhang, H., Zhang, E., He, J., Li, W., and Wei, J. (2018). Genetic Characteristics of Bacillus anthracis Isolated from Northwestern China from 1990 to 2016. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006908"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Timofeev, V., Bakhteeva, I., Khlopova, K., Mironova, R., Titareva, G., Goncharova, Y., Solomentsev, V., Kravchenko, T., Dyatlov, I., and Vergnaud, G. (2023). New Research on the Bacillus anthracis Genetic Diversity in Siberia. Pathogens, 12.","DOI":"10.3390\/pathogens12101257"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1218","DOI":"10.1016\/j.meegid.2011.05.013","article-title":"Bacillus anthracis: Molecular Taxonomy, Population Genetics, Phylogeny and Patho-Evolution","volume":"11","author":"Pilo","year":"2011","journal-title":"Infect. Genet. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"2928","DOI":"10.1128\/AEM.02574-07","article-title":"Molecular Epidemiology of Bacillus anthracis: Determining the Correct Origin","volume":"74","author":"Pilo","year":"2008","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1556\/1886.2020.00008","article-title":"Review: The Risk of Contracting Anthrax from Spore-Contaminated Soil\u2014A Military Medical Perspective","volume":"10","author":"Finke","year":"2020","journal-title":"Eur. J. Microbiol. Immunol. Bp."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1128\/jb.87.1.237-238.1964","article-title":"Isolation of Bacillus anthracis from Soil Stored 60 Years","volume":"87","author":"Wilson","year":"1964","journal-title":"J. Bacteriol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"2687","DOI":"10.3201\/eid3012.231520","article-title":"Bacteriologic and Genomic Investigation of Bacillus anthracis Isolated from World War II Site, China","volume":"30","author":"Wu","year":"2024","journal-title":"Emerg. Infect. Dis."},{"key":"ref_52","first-page":"19","article-title":"The Ecology of Anthrax in the Kruger National Park, South Africa","volume":"68S","year":"1990","journal-title":"Salisb. Med. Bull."},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Liskova, E.A., Egorova, I.Y., Selyaninov, Y.O., Razheva, I.V., Gladkova, N.A., Toropova, N.N., Zakharova, O.I., Burova, O.A., Surkova, G.V., and Malkhazova, S.M. (2021). Reindeer Anthrax in the Russian Arctic, 2016: Climatic Determinants of the Outbreak and Vaccination Effectiveness. Front. Vet. Sci., 8.","DOI":"10.3389\/fvets.2021.668420"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1007\/s10482-016-0767-5","article-title":"Virulence Plasmid Stability in Environmentally Occurring Bacillus anthracis from North East Turkey","volume":"110","author":"Cooper","year":"2016","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_55","first-page":"21","article-title":"Bacillus anthracis but Not Always Anthrax","volume":"72","author":"Turnbull","year":"1992","journal-title":"J. Appl. Bacteriol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1111\/jam.12620","article-title":"Germination and Persistence of Bacillus anthracis and Bacillus thuringiensis in Soil Microcosms","volume":"117","author":"Bishop","year":"2014","journal-title":"J. Appl. Microbiol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1303","DOI":"10.1126\/science.172.3990.1303","article-title":"Ecology of Anthrax","volume":"172","year":"1971","journal-title":"Science"},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Hsieh, H.-Y., and Stewart, G.C. (2023). Does Environmental Replication Contribute to Bacillus anthracis Spore Persistence and Infectivity in Soil?. Res. Microbiol., 174.","DOI":"10.1016\/j.resmic.2023.104052"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/1\/83\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:29:55Z","timestamp":1759919395000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/1\/83"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,16]]},"references-count":58,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["pathogens14010083"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14010083","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,16]]}}}