{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T14:32:58Z","timestamp":1792765978570,"version":"4.5.0"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T00:00:00Z","timestamp":1738540800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003243","name":"Ministry of Health of the Czech Republic","doi-asserted-by":"publisher","award":["MH CZ-DRO-VFN00064165"],"award-info":[{"award-number":["MH CZ-DRO-VFN00064165"]}],"id":[{"id":"10.13039\/501100003243","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Urinary tract infections (UTIs) are one of the most common bacterial diseases both in communities and in hospitalized patients, and at the same, time they are one of the most common indications for the use of antibiotics. UTI guidelines are generally available nationally or internationally, but they do not address all aspects of UTI treatment for different patient cohorts, age, gender, or comorbidities. The aim of the study was to point out the importance of stratified cumulative antibiograms at the level of individual health care facilities and the significant differences between epidemiological data, not only at the national level, but also at the local level. Our study analyses data from 383 patients with UTIs from a hospital department, General University Hospital (GUH), and 272 patients from an outpatient medical facility, Urocentrum (UC). This analysis focuses on the most common UTI causative agent, Escherichia coli, its representation as the causative agent of UTI in patients with complicated acute cystitis (N30), and its representation in complicated acute cystitis in patients with prostate cancer (C61). In addition to the frequency of occurrence, a sub-analysis of the incidence of resistance of E. coli to commonly used antibiotics by age, gender, diagnosis, and medical facility was performed. Results: The most common causative agent of UTI was E. coli. In patients with N30, it was 70% in GUH and 54% in UC, but in oncological patients with UTI, it was only 39% and 35%, respectively. In patients with UTI in C61, there was a significant difference in susceptibility of E. coli between individual health care facilities. Lower resistance was found in UC opposite to GUH isolates in ampicillin, with 29.8% vs. 65%, p = 0.001; amoxicillin\/clavulanic acid, with 8.5% vs. 30%, p = 0.01; with 2.1% vs. 17.5% in pivmecillinam, p = 0.01; with 10.6% vs. 37.5% in co-trimoxazole, p = 0.003; and ciprofloxacin, with 10.6% vs. 30%, p = 0.04. The study shows significant differences in the sensitivity of urinary E. coli isolates in patients in relation to age, gender, medical devices, and the presence of comorbidities.<\/jats:p>","DOI":"10.3390\/pathogens14020141","type":"journal-article","created":{"date-parts":[[2025,2,3]],"date-time":"2025-02-03T04:36:51Z","timestamp":1738557411000},"page":"141","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Role of Stratified Cumulative Antibiograms in the (Choice of Appropriate Antibiotics in Urinary Tract Infection) Management of Urinary Tract Infections"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7629-5365","authenticated-orcid":false,"given":"Vaclava","family":"Adamkova","sequence":"first","affiliation":[{"name":"Clinical Microbiology and ATB Centre, General University Hospital, 128 08 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michaela","family":"Matouskova","sequence":"additional","affiliation":[{"name":"Urocentrum Praha, 120 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2651-9527","authenticated-orcid":false,"given":"Vanda Gabriela","family":"Adamkova","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences, University of Cambridge, Cambridge CB2 3EA, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Huptych","sequence":"additional","affiliation":[{"name":"Czech Institute of Informatics, Robotics and Cybernetics (CIIRC), Czech Technical University in Prague, 160 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcela","family":"Fontana","sequence":"additional","affiliation":[{"name":"Urocentrum Praha, 120 00 Prague, Czech Republic"},{"name":"Department of Urology, University Hospital Motol, 2nd Faculty of Medicine, Charles University, 150 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1038\/nrmicro3432","article-title":"Urinary tract infections: Epidemiology, mechanisms of infection and treatment options","volume":"13","author":"Walker","year":"2015","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1756287219832172","DOI":"10.1177\/1756287219832172","article-title":"An introduction to the epidemiology and burden of urinary tract infections","volume":"11","author":"Medina","year":"2019","journal-title":"Ther. Adv. Urol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/S1047-2797(00)00072-7","article-title":"Urinary tract infection: Self-reported incidence and associated costs","volume":"10","author":"Foxman","year":"2000","journal-title":"Ann. Epidemiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"e53828","DOI":"10.2196\/53828","article-title":"Economic Burden of Community-Acquired Antibiotic-Resistant Urinary Tract Infections: Systematic Review and Meta-Analysis","volume":"10","author":"Zhu","year":"2024","journal-title":"JMIR Public Health Surveill."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"ITC49","DOI":"10.7326\/AITC201710030","article-title":"Urinary Tract Infection","volume":"167","author":"Gupta","year":"2017","journal-title":"Ann. Intern. Med."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.eururo.2024.03.035","article-title":"European Association of Urology Guidelines on Urological Infections: Summary of the 2024 Guidelines","volume":"86","author":"Kranz","year":"2024","journal-title":"Eur. Urol."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Huang, L., Huang, C., Yan, Y., Sun, L., and Li, H. (2022). Urinary Tract Infection Etiological Profiles and Antibiotic Resistance Patterns Varied Among Different Age Categories: A Retrospective Study From a Tertiary General Hospital During a 12-Year Period. Front. Microbiol., 12.","DOI":"10.3389\/fmicb.2021.813145"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"242","DOI":"10.1080\/00325481.2017.1246055","article-title":"An update on the management of urinary tract infections in the era of antimicrobial resistance","volume":"129","author":"Bader","year":"2017","journal-title":"Postgrad. Med."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Hrbacek, J., Cermak, P., and Zachoval, R. (2020). Current Antibiotic Resistance Trends of Uropathogens in Central Europe: Survey from a Tertiary Hospital Urology Department 2011\u20132019. Antibiotics, 9.","DOI":"10.3390\/antibiotics9090630"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.ijid.2022.03.052","article-title":"Regional Differences in Antibiotic-resistant Enterobacterales Urine Isolates in the United States: 2018\u20132020","volume":"119","author":"Aronin","year":"2022","journal-title":"Int. J. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"164","DOI":"10.1093\/cid\/cit688","article-title":"Appropriate antibiotic use for patients with urinary tract infections reduces length of hospital stay","volume":"58","author":"Spoorenberg","year":"2014","journal-title":"Clin. Infect. Dis."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"502","DOI":"10.1111\/j.1469-0691.2004.00911.x","article-title":"Optimal use of antibiotic resistance surveillance systems","volume":"10","author":"Critchley","year":"2004","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"World Health Organisation (WHO) (2007). Global action plan on antimicrobial resistance. Geneva: WHO; 2015. Available from Hindler JF, Stelling J. Analysis and presentation of cumulative antibiograms: A new consensus guideline from the Clinical and Laboratory Standards Institute. Clin. Infect. Dis., 44, 867\u2013873.","DOI":"10.1086\/511864"},{"key":"ref_14","unstructured":"Eden, C., and Ackermann, F. (2013). Making Strategy: The Journey of Strategic Management, SAGE Publications, Inc."},{"key":"ref_15","unstructured":"CLSI (2014). Analysis and Presentation of Cumulative Antimicrobial Susceptibility Test Data, Clinical and Laboratory Standards Institute. Approved Guideline-Fourth Edition; CLSI document M39-A4."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2977","DOI":"10.1128\/JCM.01077-15","article-title":"Challenges in Preparation of Cumulative Antibiogram Reports for Community Hospitals","volume":"53","author":"Moehring","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"E159","DOI":"10.25011\/cim.v30i4.1777","article-title":"Investigation of sources of potential bias in laboratory surveillance for anti-microbial resistance","volume":"30","author":"Laupland","year":"2007","journal-title":"Clin. Investig. Med."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Klinker, K.P., Hidayat, L.K., DeRyke, C.A., DePestel, D.D., Motyl, M., and Bauer, K.A. (2021). Antimicrobial stewardship and antibiograms: Importance of moving beyond traditional antibiograms. Ther. Adv. Infect. Dis., 8.","DOI":"10.1177\/20499361211011373"},{"key":"ref_19","unstructured":"(2024, December 10). Available online: https:\/\/www.who.int\/publications\/i\/item\/9789241509763."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Simner, P.J., Hindler, J.A., Bhowmick, T., Das, S., Johnson, J.K., Lubers, B.V., Redell, M.A., Stelling, J., and Erdman, S.M. (2022). What\u2019s New in Antibiograms? Updating CLSI M39 Guidance with Current Trends. J. Clin. Microbiol., 60.","DOI":"10.1128\/jcm.02210-21"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"268","DOI":"10.1111\/j.1469-0691.2011.03570.x","article-title":"Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: An international expert proposal for interim standard definitions for acquired resistance","volume":"18","author":"Magiorakos","year":"2012","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1803","DOI":"10.1093\/cid\/ciy378","article-title":"Difficult-to-Treat Resistance in Gram-negative Bacteremia at 173 US Hospitals: Retrospective Cohort Analysis of Prevalence, Predictors, and Outcome of Resistance to All First-line Agents","volume":"67","author":"Kadri","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Neugebauer, M., Ebert, M., and Vogelmann, R. (2020). A clinical decision support system improves antibiotic therapy for upper urinary tract infection in a randomized single-blinded study. BMC Health Serv. Res., 20.","DOI":"10.1186\/s12913-020-5045-6"},{"key":"ref_24","unstructured":"Wein, A., and Kavoussi, L. (2012). Infections of the urinary tract. Campbell-Walsh Urology, Elsevier Saunders. [10th ed.]."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1128\/JCM.02069-13","article-title":"Characterization of Escherichia coli isolates from hospital inpatients or outpatients with urinary tract infection","volume":"52","author":"Toval","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1177\/03915603241236361","article-title":"Spectrum and antibiotic resistance in community- and hospital-acquired urinary tract infections among adults: Experience from a large tertiary care center in a developing country","volume":"91","author":"Herbawi","year":"2024","journal-title":"Urologia"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Cepas, V., and Soto, S.M. (2020). Relationship between Virulence and Resistance among Gram-Negative Bacteria. Antibiotics, 9.","DOI":"10.3390\/antibiotics9100719"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Critchley, I.A., Cotroneo, N., Pucci, M.J., and Mendes, R. (2019). The burden of antimicrobial resistance among urinary tract isolates of Escherichia coli in the United States in 2017. PLoS ONE, 14.","DOI":"10.1101\/703199"},{"key":"ref_29","unstructured":"Matou\u0161kov\u00e1, M., Ad\u00e1mkov\u00e1, V., \u010cechov\u00e1, M., and Lahoda Brodsk\u00e1, H. (2022). Konsenzu\u00e1ln\u00ed Postupy v L\u00e9\u010db\u011b Mo\u010dov\u00fdch Infekc\u00ed, Solen."},{"key":"ref_30","unstructured":"(2024, December 15). Available online: https:\/\/szu.gov.cz\/wp-content\/uploads\/2023\/12\/PSMR_2021_EC.pdf."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Corse, L., and Cartwright, A. (2024). Investigating trends in antibiotic resistance of Escherichia coli isolated from clinical urine specimens in the Orkney Islands. Microbiology, 170.","DOI":"10.1099\/mic.0.001514"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2629","DOI":"10.1128\/JCM.00481-17","article-title":"Emergency Department Urinary Antibiograms Differ by Specific Patient Group","volume":"55","author":"Jorgensen","year":"2017","journal-title":"J. Clin. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e01350-17","DOI":"10.1128\/AAC.01350-17","article-title":"In Vitro Comparison of Ceftolozane-Tazobactam to Traditional Beta-Lactams and Ceftolozane-Tazobactam as an Alternative to Combination Antimicrobial Therapy for Pseudomonas aeruginosa","volume":"61","author":"Goodlet","year":"2017","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"dlaa129","DOI":"10.1093\/jacamr\/dlaa129","article-title":"Collective assessment of antimicrobial susceptibility among the most common Gram-negative respiratory pathogens driving therapy in the ICU","volume":"3","author":"Moise","year":"2021","journal-title":"JAC Antimicrob. Resist."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"ofae643","DOI":"10.1093\/ofid\/ofae643","article-title":"A Comparison of Different Strategies for Optimizing the Selection of Empiric Antibiotic Therapy for Pneumonia Caused by Gram-Negative Bacteria in Intensive Care Units: Unit-Specific Combination Antibiograms Versus Patient-Specific Risk Factors","volume":"11","author":"Wangchinda","year":"2024","journal-title":"Open Forum Infect. Dis."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1992","DOI":"10.1093\/cid\/ciab560","article-title":"Antimicrobial Resistance Trends in Urine Escherichia coli Isolates From Adult and Adolescent Females in the United States From 2011 to 2019: Rising ESBL Strains and Impact on Patient Management","volume":"73","author":"Kaye","year":"2021","journal-title":"Clin. Infect. Dis."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Golli, A.-L., Zlatian, O.M., Cara, M.L., and Olteanu, M. (2024). Pre- and Post-COVID-19 Antimicrobial Resistance Pattern of Pathogens in an Intensive Care Unit. Pharmaceuticals, 17.","DOI":"10.20944\/preprints202402.0311.v1"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"733","DOI":"10.1007\/s11845-016-1538-z","article-title":"Antibiotic resistance patterns of Escherichia coli urinary isolates and comparison with antibiotic consumption data over 10 years, 2005\u20132014","volume":"186","author":"Stapleton","year":"2017","journal-title":"Ir. J. Med. Sci."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1080\/02813432.2019.1569425","article-title":"Prescription of antibiotics for urinary tract infection in general practice in Denmark","volume":"37","author":"Holm","year":"2019","journal-title":"Scand. J. Prim. Health Care"},{"key":"ref_40","unstructured":"(2024, December 15). Central Coordination Group of the National End of the Czech Republic: Antibiotic consumption in the Czech Republic in 2008\u20132018\u2014Part 1. Pharmacotherapeutic Information 1\/2020. (In Czech)."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/2\/141\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:26:07Z","timestamp":1760027167000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/2\/141"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,3]]},"references-count":40,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["pathogens14020141"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14020141","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,3]]}}}