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N\u00edvel Superior (CAPES)","award":["2020\/07826-5"],"award-info":[{"award-number":["2020\/07826-5"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["2020\/12037-0"],"award-info":[{"award-number":["2020\/12037-0"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["2022\/16555-0"],"award-info":[{"award-number":["2022\/16555-0"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["19\/2551\u20130001842\u20138"],"award-info":[{"award-number":["19\/2551\u20130001842\u20138"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["24\/2551\u201300011579"],"award-info":[{"award-number":["24\/2551\u201300011579"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["303701\/2021-8"],"award-info":[{"award-number":["303701\/2021-8"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["311769\/2023-3"],"award-info":[{"award-number":["311769\/2023-3"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["312576\/2021-8"],"award-info":[{"award-number":["312576\/2021-8"]}]},{"name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior (CAPES)","award":["001"],"award-info":[{"award-number":["001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Domestic and wild felids are frequently parasitized by apicomplexan protozoa in the genus Cytauxzoon. Expanding species diversity has recently been described within this genus, with potential implications for epidemiology and pathogenesis. In light of these findings, this study assessed the genetic diversity of Cytauxzoon spp. in wild felids (n = 66) from different eco-regions of Brazil and Argentina. Of the 66 blood samples analyzed, 53 (80.3%) were 18S rRNA gene PCR-positive for Cytauxzoon spp., including 43 jaguars (Panthera onca) and 10 ocelots (Leopardus pardalis). Panthera onca specimens (100%, 43\/43) were most frequently infected, followed by Leopardus pardalis (76.9%; 10\/13). Cytauxzoon spp. were not detected in Leopardus braccatus (n = 1) or Puma concolor (n = 9). Phylogenetic analyses of fragments of the 18S rRNA, cytB, and cox-1 gene sequences from jaguars were closely related to Cytauxzoon felis. In contrast, sequences from ocelots were more closely associated with Cytauxzoon brasiliensis. Distance and haplotype analysis further confirmed the circulation of at least two distinct genovariants of C. felis among jaguars, as evidenced by their close positioning and low genetic divergence (0\u20130.14% for 18S rRNA, 0.37\u20130.56% for cytB, and 0.08\u20130.74% for cox-1). Additionally, sequence data from ocelots suggested that multiple genovariants of C. brasiliensis are circulating among these cats in different Brazilian eco-regions. Our study provides evidence of two distinct Cytauxzoon organisms parasitizing free-ranging and captive jaguars and ocelots, respectively, in Brazil and Argentina.<\/jats:p>","DOI":"10.3390\/pathogens14020148","type":"journal-article","created":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T07:41:44Z","timestamp":1738654904000},"page":"148","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Diversity of Cytauxzoon spp. (Piroplasmida: Theileriidae) in Wild Felids from Brazil and Argentina"],"prefix":"10.3390","volume":"14","author":[{"given":"Ana Cl\u00e1udia","family":"Calchi","sequence":"first","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences (FCAV), S\u00e3o Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0007-9690","authenticated-orcid":false,"given":"Joares A.","family":"May-J\u00fanior","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Protozoologia e Rickettsioses Vetoriais (ProtoZooVet), Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91540-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8077-5070","authenticated-orcid":false,"given":"Vin\u00edcius","family":"Baggio-Souza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Protozoologia e Rickettsioses Vetoriais (ProtoZooVet), Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91540-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3155-8969","authenticated-orcid":false,"given":"Laura","family":"Berger","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Protozoologia e Rickettsioses Vetoriais (ProtoZooVet), Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91540-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2244-9848","authenticated-orcid":false,"given":"Renata","family":"Fagundes-Moreira","sequence":"additional","affiliation":[{"name":"National PhD Program in One Health Approaches to Infectious Diseases and Life Science Research, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, 27100 Pavia, Italy"},{"name":"Department of Veterinary Medicine, University of Bari \u201cAldo Moro\", 70121 Valenzano, 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Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3506-0279","authenticated-orcid":false,"given":"Edward Bealmear","family":"Breitschwerdt","sequence":"additional","affiliation":[{"name":"Intracellular Pathogens Research Laboratory, College of Veterinary Medicine, Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7428-0138","authenticated-orcid":false,"given":"Ricardo G.","family":"Maggi","sequence":"additional","affiliation":[{"name":"Intracellular Pathogens Research Laboratory, College of Veterinary Medicine, Comparative Medicine Institute, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9658-0999","authenticated-orcid":false,"given":"Eduardo","family":"Eizirik","sequence":"additional","affiliation":[{"name":"Laboratory of Genomics and Molecular Biology, School of Health and Life Sciences, Pontif\u00edcia Universidade Cat\u00f3lica do Rio Grande do Sul, Porto Alegre 90619-900, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosangela Zacarias","family":"Machado","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences (FCAV), S\u00e3o Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0819-6290","authenticated-orcid":false,"given":"Fabiana Lopes","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Mam\u00edferos, Federal University of Para\u00edba (UFPB), Jo\u00e3o Pessoa 58051-900, PB, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Fabio","family":"Soares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Protozoologia e Rickettsioses Vetoriais (ProtoZooVet), Faculdade de Veterin\u00e1ria, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre 91540-000, RS, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-5222","authenticated-orcid":false,"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agricultural and Veterinarian Sciences (FCAV), S\u00e3o Paulo State University (UNESP), Jaboticabal 14884-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Wikander, Y.M., and Reif, K.E. (2023). Cytauxzoon felis: An overview. Pathogens, 12.","DOI":"10.3390\/pathogens12010133"},{"key":"ref_2","first-page":"63","article-title":"Cytauxzoon sylvicaprae gen. nov., spec. nov., a protozoon responsible for a hitherto undescribed disease in the duiker, Sylvicapra grimmia (Linn\u00e9)","volume":"23","author":"Neitz","year":"1948","journal-title":"Onderstepoort J. Vet. Sci. Anim. Ind."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1207","DOI":"10.1007\/s00436-022-07424-8","article-title":"The Piroplasmida Babesia, Cytauxzoon, and Theileria in farm and companion animals: Species compilation, molecular phylogeny, and evolutionary insights","volume":"121","author":"Schnittger","year":"2022","journal-title":"Parasitol. Res."},{"key":"ref_4","first-page":"874","article-title":"Cytauxzoonosis in domestic cats (Felis domestica) in Missouri","volume":"167","author":"Wagner","year":"1975","journal-title":"J. Am. Vet. Med. 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Parasitol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1017\/S0031182014001504","article-title":"Molecular characterization of Babesia and Cytauxzoon species in wild South-African meerkats","volume":"142","author":"Leclaire","year":"2015","journal-title":"Parasitology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.vetpar.2010.06.018","article-title":"Molecular evidence of the multiple genotype infection of a wild Hokkaido brown bear (Ursus arctos yesoensis) by Babesia sp. UR1","volume":"173","author":"Jinnai","year":"2010","journal-title":"Vet. Parasitol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"3739","DOI":"10.1007\/s00436-020-06873-3","article-title":"Molecular detection of apicomplexan protozoa in Hokkaido brown bears (Ursus arctos yesoensis) and Japanese black bears (Ursus thibetanus japonicus)","volume":"119","author":"Moustafa","year":"2020","journal-title":"Parasitol. Res."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"420","DOI":"10.1645\/GE-384R","article-title":"A new species of Cytauxzoon from Pallas\u2019 cats caught in Mongolia and comments on the systematics and taxonomy of piroplasmids","volume":"91","author":"Reichard","year":"2005","journal-title":"J. Parasitol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Reichard, M.V., Sanders, T.L., Weerarathne, P., Meinkoth, J.H., Miller, C.A., Scimeca, R.C., and Almaz\u00e1n, C. (2021). Cytauxzoonosis in North America. Pathogens, 10.","DOI":"10.3390\/pathogens10091170"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1177\/1098612X15589878","article-title":"Cytauxzoonosis in cats: ABCD guidelines on prevention and management","volume":"17","author":"Lloret","year":"2015","journal-title":"J. Feline Med. 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