{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:21:50Z","timestamp":1791591710297,"version":"4.3.4"},"reference-count":15,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T00:00:00Z","timestamp":1738800000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>During pregnancy, primary Toxoplasma gondii infection can cause congenital toxoplasmosis (CT). We described the newborns\u2019 outcomes from a multicentre cohort of mothers with seroconversion (SC) at different gestational ages. This retrospective observational study (from 2007 to 2018) was conducted in two Italian referral hospitals: Fondazione IRCCS Policlinico San Matteo in Pavia and Spedali Civili in Brescia. In total, 247 pregnant women were enrolled: seroconversions were enrolled: seroconversions documented as having occurred in the two months preceding pregnancy in 12 cases (4.9%; 95% CI 2.2\u20137.5%), and during pregnancy in 235 cases (95.1%; 95% CI 92.5\u201397.8%). SC is defined as the appearance of specific anti-Toxoplasma antibodies (IgM\/IgG) during pregnancy in a previously seronegative woman. A total of 56 (22.5%; 95% CI 17.3\u201327.7%) newborns were lost to follow-up; thus, the outcome of 193 (77.5%; 95% CI 72.3\u201382.7%) newborns was analyzed. The overall transmission rate of T. gondii infection was 23.8% (95% CI 17.8\u201329.8%), 0% (95% CI 0.0\u201311.9%) among the 1st trimester SCs, 12.5% (95% CI 5.6\u201319.4%) among the 2nd trimester SCs, 53.8% (95% CI 41.7\u201366.0%) among the 3rd trimester ones. No CT were found in the group of periconceptional infection. Among the infected newborns, clinically manifest cases were 12 (26.1%; 95% CI 13.4\u201338.8%), including 1 case (2.2%; 95% CI 2.0\u20136.4%) of stillbirth and 11 symptomatic neonates (23.9%; 95% CI 11.6\u201336.2%). A total of 83 amniocentesis were performed (33.6%; 95% CI 27.7\u201339.5%), no complication was recorded and no false positive or false negative results were registered. The results are in line with the fetal risks reported in literature for T. gondii infection during pregnancy, even if at a lower percentage probably due to a prompt treatment.<\/jats:p>","DOI":"10.3390\/pathogens14020157","type":"journal-article","created":{"date-parts":[[2025,2,6]],"date-time":"2025-02-06T11:32:32Z","timestamp":1738841552000},"page":"157","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Risk of Congenital Toxoplasmosis in Newborns from Mothers with Documented Infection: Experience from Two Referral Centres"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7304-0391","authenticated-orcid":false,"given":"Alice","family":"Bonetti","sequence":"first","affiliation":[{"name":"Microbiology and Virology Unit, Diagnostic Medicine Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy"},{"name":"PhD National Programme in One Health Approaches to Infectious Diseases and Life Science Research, Department of Public Health, Experimental and Forensic Medicine, University of Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4817-8337","authenticated-orcid":false,"given":"Agnese","family":"Comelli","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Foundation IRCCS Ca\u2019 Granda Ospedale Maggiore Policlinico, 20122 Milan, Italy"},{"name":"Department of Infectious and Tropical Diseases, ASST Spedali Civili, 25123 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7109-4399","authenticated-orcid":false,"given":"Annacarla","family":"Chiesa","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Manzoni Hospital, 23900 Lecco, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vania","family":"Spinoni","sequence":"additional","affiliation":[{"name":"Department of Neonatology and Neonatal Intensive Care, Spedali Civili Hospital, Spedali Civili, 25123 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9657-5084","authenticated-orcid":false,"given":"Ambra","family":"Vola","sequence":"additional","affiliation":[{"name":"Microbiology and Virology Unit, Diagnostic Medicine Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7793-714X","authenticated-orcid":false,"given":"Federico","family":"Prefumo","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Unit, IRCCS Istituto Giannina Gaslini, 16147 Genova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriana","family":"Valcamonico","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, University of Brescia, 25123 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9851-6598","authenticated-orcid":false,"given":"Carlo","family":"Bonfanti","sequence":"additional","affiliation":[{"name":"Department of Molecular and Translational Medicine, Institute of Microbiology, University of Brescia-ASST Spedali Civili, 25123 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvio","family":"Caligaris","sequence":"additional","affiliation":[{"name":"Department of Molecular and Translational Medicine, Institute of Microbiology, University of Brescia-ASST Spedali Civili, 25123 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lina Rachele","family":"Tomasoni","sequence":"additional","affiliation":[{"name":"Department of Infectious and Tropical Diseases, ASST Spedali Civili, 25123 Brescia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fausto","family":"Baldanti","sequence":"additional","affiliation":[{"name":"Microbiology and Virology Unit, Diagnostic Medicine Department, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy"},{"name":"Dipartimento di Scienze Clinico-Chirurgiche, Diagnostiche e Pediatriche Universit\u00e0 di Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeria","family":"Meroni","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine, University of Pavia, 27100 Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,6]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"264","DOI":"10.1128\/CMR.05013-11","article-title":"Epidemiology of and diagnostic strategies for toxoplasmosis","volume":"25","year":"2012","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1046\/j.1469-0691.2002.00485.x","article-title":"Toxoplasma gondii: Transmission, diagnosis and prevention","volume":"8","author":"Hill","year":"2002","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Peyron, F., L\u2019ollivier, C., Mandelbrot, L., Wallon, M., Piarroux, R., Kieffer, F., Hadjadj, E., Paris, L., and Garcia-Meric, P. (2019). Maternal and Congenital Toxoplasmosis: Diagnosis and Treatment Recommendations of a French Multidisciplinary Working Group. Pathogens, 8.","DOI":"10.3390\/pathogens8010024"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"761.e9","DOI":"10.1016\/j.cmi.2018.09.024","article-title":"Risk of congenital toxoplasmosis in women with low or indeterminate anti-Toxoplasma IgG avidity index in the first trimester of pregnancy: An observational retrospective study","volume":"25","author":"Tomasoni","year":"2018","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1093\/cid\/cit032","article-title":"Congenital Toxoplasma Infection: Monthly Prenatal Screening Decreases Transmission Rate and Improves Clinical Outcome at Age 3 Years","volume":"56","author":"Wallon","year":"2013","journal-title":"Clin. Infect. Dis."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Gomes Ferrari Strang, A.G., Ferrar, R.G., and Falavigna-Guilherme, A.L. (2023). Gestational toxoplasmosis treatment changes the child\u2019s prognosis: A cohort study in southern Brazil. PLoS Neglected Trop. Dis., 17.","DOI":"10.1371\/journal.pntd.0011544"},{"key":"ref_7","unstructured":"(2024, November 22). Italian Guidelines Approved by AMCLI. Available online: www.amcli.it."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1186\/s40001-021-00606-7","article-title":"A fresh look at the role of spiramycin in preventing a neglected disease: Meta-analyses of observational studies","volume":"26","author":"Montoya","year":"2021","journal-title":"Eur. J. Med. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1545","DOI":"10.1093\/cid\/cis234","article-title":"Efficacy of Rapid Treatment Initiation Following Primary Toxoplasma gondii Infection During Pregnancy","volume":"54","author":"Hotop","year":"2012","journal-title":"Clin. Infect. Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"386-e1","DOI":"10.1016\/j.ajog.2018.05.031","article-title":"Prenatal therapy with pyrimethamine + sulfadiazine versus spiramycin to reduce placental transmission of toxoplasmosis: A multicentre, randomized trial","volume":"219","author":"Mandelbrot","year":"2018","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"695","DOI":"10.1056\/NEJM199409153311102","article-title":"Prenatal Diagnosis of Congenital Toxoplasmosis with a Polymerase-Chain-Reaction Test on Amniotic Fluid","volume":"331","author":"Hohlfeld","year":"1994","journal-title":"N. Engl. J. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1016\/j.diagmicrobio.2013.02.008","article-title":"Contribution of molecular diagnosis to congenital toxoplasmosis","volume":"76","author":"Delhaes","year":"2013","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1529","DOI":"10.1080\/14787210.2021.1941867","article-title":"Molecular diagnosis of toxoplasmosis: Recent advances and a look to the future","volume":"19","author":"Robert","year":"2021","journal-title":"Expert Rev. Anti-Infect. Ther."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Bollani, L., Auriti, C., Achille, C., Garofoli, F., De Rose, D.U., Meroni, V., Salvatori, G., and Tzialla, C. (2022). Congenital Toxoplasmosis: The State of the Art. Front. Pediatr., 10.","DOI":"10.3389\/fped.2022.894573"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Ruggiero, M., Testa, L., Ronchi, A., Meroni, V., Pugni, L., Ronchi, A., Pietrasanta, C., Somigliana, E., and Tassis, B. (2024). Antenatal toxoplasmosis screening and treatment in Northern Italy: Update on the clinical effectiveness. Minerva Obstet. Gynecol.","DOI":"10.23736\/S2724-606X.24.05471-X"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/2\/157\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:28:20Z","timestamp":1760027300000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/2\/157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,6]]},"references-count":15,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2025,2]]}},"alternative-id":["pathogens14020157"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14020157","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,2,6]]}}}