{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T00:57:43Z","timestamp":1760057863285,"version":"build-2065373602"},"reference-count":87,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T00:00:00Z","timestamp":1740441600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100005825","name":"USDA NIFA","doi-asserted-by":"publisher","award":["Hatch CT0477","2022-67013-36140","404937\/2021\u20137","304757\/2022-5","APQ-1073\u20135.01\/21"],"award-info":[{"award-number":["Hatch CT0477","2022-67013-36140","404937\/2021\u20137","304757\/2022-5","APQ-1073\u20135.01\/21"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico\u2014CNPq","doi-asserted-by":"publisher","award":["Hatch CT0477","2022-67013-36140","404937\/2021\u20137","304757\/2022-5","APQ-1073\u20135.01\/21"],"award-info":[{"award-number":["Hatch CT0477","2022-67013-36140","404937\/2021\u20137","304757\/2022-5","APQ-1073\u20135.01\/21"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Funda\u00e7\u00e3o de Amparo a Ci\u00eancia e Tecnologia do Estado de Pernambuco\u2014FACEPE","award":["Hatch CT0477","2022-67013-36140","404937\/2021\u20137","304757\/2022-5","APQ-1073\u20135.01\/21"],"award-info":[{"award-number":["Hatch CT0477","2022-67013-36140","404937\/2021\u20137","304757\/2022-5","APQ-1073\u20135.01\/21"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The prevalence of antimicrobial resistance (AMR) in bacterial pathogens resulting from the intensive usage of antibiotics and antibiotic compounds is acknowledged as a significant global concern that impacts both human and animal health. In this study, we sequenced and analyzed the genomes of two emergent onion-pathogenic species of Burkholderia, B. cenocepacia CCRMBC56 and B. orbicola CCRMBC23, focusing on genes that are potentially associated with their high level of antibiotic and copper resistance. We also identified genes contributing to the copper resistance of B. cenocepacia CCRMBC56 through high-throughput analysis of mutated genes in random transposon mutant populations that were differentially enriched in a copper-containing medium. The results indicated that genes involved in DNA integration, recombination, and cation transport are important for the survival of B. cenocepacia CCRMBC56 in copper-stressed conditions. Furthermore, the fitness effect analysis identified additional genes crucial for copper resistance, which are involved in functions associated with the oxidative stress response, the ABC transporter complex, and the cell outer membrane. In the same analysis, genes related to penicillin binding, the TCA cycle, and FAD binding were found to hinder bacterial adaptation to copper toxicity. This study provides potential targets for reducing the copper resistance of B. cenocepacia and other copper-resistant bacterial pathogens.<\/jats:p>","DOI":"10.3390\/pathogens14030226","type":"journal-article","created":{"date-parts":[[2025,2,25]],"date-time":"2025-02-25T10:55:48Z","timestamp":1740480948000},"page":"226","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization of the Antibiotic and Copper Resistance of Emergent Species of Onion-Pathogenic Burkholderia Through Genome Sequence Analysis and High-Throughput Sequencing of Differentially Enriched Random Transposon Mutants"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2708-1173","authenticated-orcid":false,"given":"Jonas J.","family":"Padilla","sequence":"first","affiliation":[{"name":"Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6153-1700","authenticated-orcid":false,"given":"Marco A. S.","family":"da Gama","sequence":"additional","affiliation":[{"name":"Department of Agronomy, Universidade Federal Rural de Pernambuco, Recife 52171-900, PE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inderjit","family":"Barphagha","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8924-0091","authenticated-orcid":false,"given":"Jong Hyun","family":"Ham","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Crop Physiology, Louisiana State University Agricultural Center, Baton Rouge, LA 70803, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,2,25]]},"reference":[{"key":"ref_1","first-page":"e47858","article-title":"Antibiotic Prophylaxis in Surgery: Current Insights and Future Directions for Surgical Site Infection Prevention","volume":"15","author":"Dhole","year":"2023","journal-title":"Cureus"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Tang, K.W.K., Millar, B.C., and Moore, J.E. 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