{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T03:05:30Z","timestamp":1784689530179,"version":"3.55.0"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T00:00:00Z","timestamp":1741046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100011011","name":"Junta de Andaluc\u00eda (Spain)","doi-asserted-by":"publisher","award":["Bio-243"],"award-info":[{"award-number":["Bio-243"]}],"id":[{"id":"10.13039\/501100011011","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>A survey was conducted to assess the impact of on-board gutting in the pouting fishery, Trisopterus luscus (L.), from the Bay of Biscay (area FAO 27.VIII) on the parasite burden of macroscopic ascaridoid nematodes, including anisakids (causing anisakidosis) and raphidascaridids (causing consumer rejection) in these fish. The fish were caught in the Bay of Biscay and collected from the fish market in Granada (southern Spain). Fish larger than 25 cm were gutted on board after capture. A detailed examination of the fish revealed the presence of nematode larvae, which were identified morphologically and molecularly (PCR-RFLP: polymerase chain reaction with restriction fragment polymorphism). Results revealed that ungutted fish harbored only third-stage larvae of ascaridoids (Anisakis and Hysterothylacium) while prevalence reached up to 91%. In contrast, gutted fish exhibited a significant reduction in both the prevalence (36%) and mean abundance (MA, 4.44 vs. 0.91) of these larvae. The prevalence of Anisakis spp. larvae was reduced by over 20%, with a more pronounced reduction in abundance of more than 40% (MA, 1.56 vs. 0.91). Hysterothylacium larvae were completely absent (MA 2.88 vs. 0.00). These findings indicate that gutting, while not highly efficient, lowers Anisakis larvae presence, thereby reducing the risk of anisakiasis to consumers. Additionally, the complete removal of Hysterothylacium larvae enhances the fish\u2019s appearance, making it more appealing and increasing its commercial value, as well as reducing the risk of seizure by health authorities. Further research on these on-board evisceration practices is needed to enhance effectiveness and reduce zoonotic nematodes in commercial fishes.<\/jats:p>","DOI":"10.3390\/pathogens14030252","type":"journal-article","created":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T04:58:17Z","timestamp":1741064297000},"page":"252","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["First Insights from On-Board Fish Gutting into the Zoonotic Nematode Burden of Pouting (Trisopterus luscus) at the Point of Sale to the Consumer"],"prefix":"10.3390","volume":"14","author":[{"given":"Francisco Javier","family":"Arrebola-Casa\u00f1as","sequence":"first","affiliation":[{"name":"Departamento de Parasitolog\u00eda, Facultad de Farmacia, Universidad de Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7899-8913","authenticated-orcid":false,"given":"Mario","family":"Garrido","sequence":"additional","affiliation":[{"name":"Department of Biology and Geology, Physics and Inorganic Chemistry, Biodiversity and Conservation Area, Rey Juan Carlos University, M\u00f3stoles, 28933 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7969-6658","authenticated-orcid":false,"given":"Francisco Javier","family":"Adroher","sequence":"additional","affiliation":[{"name":"Departamento de Parasitolog\u00eda, Facultad de Farmacia, Universidad de Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4680-1148","authenticated-orcid":false,"given":"Roc\u00edo","family":"Ben\u00edtez","sequence":"additional","affiliation":[{"name":"Departamento de Parasitolog\u00eda, Facultad de Farmacia, Universidad de Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8414-5052","authenticated-orcid":false,"given":"Manuel","family":"Morales-Yuste","sequence":"additional","affiliation":[{"name":"Departamento de Parasitolog\u00eda, Facultad de Farmacia, Universidad de Granada, 18071 Granada, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1016\/j.rvsc.2020.08.003","article-title":"Anisakiasis and Anisakis: An underdiagnosed emerging disease and its main etiological agents","volume":"132","year":"2020","journal-title":"Res. Vet. Sci."},{"key":"ref_2","first-page":"43","article-title":"Anisakidae and Anisakidosis","volume":"Volume 3","author":"Sun","year":"1993","journal-title":"Progress in Clinical Parasitology"},{"key":"ref_3","first-page":"12","article-title":"Female worm Hysterothylacium aduncum excreted from human: A case report","volume":"45","author":"Yagi","year":"1996","journal-title":"Jpn J. Parasitol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1128\/CMR.00012-07","article-title":"Anisakis simplex: From obscure infectious worm to inducer of immune hypersensitivity","volume":"21","author":"Kennedy","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_5","unstructured":"Froese, R., and Pauly, D. (2025, January 15). Trisopterus Luscus. Available online: https:\/\/fishbase.mnhn.fr\/summary\/Trisopterus-luscus.html."},{"key":"ref_6","unstructured":"Ubeira, F.M., Vali\u00f1as, B., Lorenzo, S., Iglesias, R., Figueiras, A., and Garc\u00eda-Villaescusa, R. (2000). Anisaquiose e Alerxia. Un Estudio Seroepidemiol\u00f3xico na Comunidade Aut\u00f3noma Galega, Conseller\u00eda de Sanidade e Servicios Sociais (Xunta de Galicia)."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1017\/S0022149X08006196","article-title":"Anisakid parasites of the pouting (Trisopterus luscus) from the Cantabrian Sea coast, Bay of Biscay, Spain","volume":"82","author":"Rello","year":"2008","journal-title":"J. Helminthol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"492","DOI":"10.1007\/s004360100389","article-title":"Anisakis infestation in marine fish and cephalopods from Galician waters: An updated perspective","volume":"87","author":"Abollo","year":"2001","journal-title":"Parasitol. Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1016\/j.rvsc.2023.02.007","article-title":"Anisakis infection in anchovies (Engraulis encrasicolus) from Iberian waters, southwestern Europe: Post-mortem larval migration","volume":"157","author":"Adroher","year":"2023","journal-title":"Res. Vet. Sci."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"783","DOI":"10.4315\/0362-028X-56.9.783","article-title":"Catch handling and the possible migration of Anisakis larvae in herring, Clupea harengus","volume":"56","author":"Roepstorff","year":"1993","journal-title":"J. Food Prot."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/00364827.1961.10410245","article-title":"Nematodes from some Norwegian marine fishes","volume":"2","author":"Berland","year":"1961","journal-title":"Sarsia"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"347","DOI":"10.5962\/p.292212","article-title":"Larves d\u2019ascarides parasites de poissons en M\u00e9diterran\u00e9e occidentale","volume":"10A","author":"Petter","year":"1988","journal-title":"Bull. Mus\u00e9um Natl. Histoire Nat. Paris 4e S\u00e9rie"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.fishres.2016.12.011","article-title":"UV-press method versus artificial digestion method to detect Anisakidae L3 in fish fillets: Comparative study and suitability for the industry","volume":"202","author":"Castro","year":"2018","journal-title":"Fish. Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1139\/f70-107","article-title":"Use of ultraviolet light to find parasitic nematodes in situ","volume":"27","author":"Pippy","year":"1970","journal-title":"J. Fish. Res. Board Can."},{"key":"ref_15","unstructured":"Monstad, T. (1989, January 12\u201316). Some aspects of mortality, condition factors and liver state with Anisakis-infection in blue whiting in the North-East Atlantic. Proceedings of the Fourth Soviet-Norwegian Symposium, Bergen, Norway."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1111\/j.1439-0426.2006.00805.x","article-title":"Cube law, condition factor and weight-length relationships: History, meta-analysis and recommendations","volume":"22","author":"Froese","year":"2006","journal-title":"J. Appl. Ichthyol."},{"key":"ref_17","first-page":"141","article-title":"The rate of growth of fishes","volume":"3","author":"Fulton","year":"1904","journal-title":"22nd Annu. Rep. Fish. Board Scotl."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1911","DOI":"10.1016\/S0020-7519(98)00150-7","article-title":"Characterisation of anisakid nematodes with zoonotic potential by nuclear ribosomal DNA sequences","volume":"28","author":"Zhu","year":"1998","journal-title":"Int. J. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Buzo-Dom\u00ednguez, S., Morales-Yuste, M., Domingo-Hern\u00e1ndez, A.M., Ben\u00edtez, R., and Adroher, F.J. (2021). Molecular epidemiology of Anisakis spp. in wedge sole, Dicologlossa cuneata (Moreau, 1881), from fishmarkets in Granada (southern Spain), caught in two adjacent NE and CE Atlantic areas. Pathogens, 10.","DOI":"10.3390\/pathogens10101302"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"e13989","DOI":"10.1111\/jfd.13989","article-title":"Mullus barbatus L. and Mullus surmuletus L. from western Mediterranean waters (SE Spain) are infected by Hysterothylacium fabri, but not by zoonotic nematodes. Possible impact on fish hosts","volume":"47","author":"Veiga","year":"2024","journal-title":"J. Fish Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.ijfoodmicro.2015.02.024","article-title":"Fishing area and fish size as risk factors of Anisakis infection in sardines (Sardina pilchardus) from Iberian waters, southwestern Europe","volume":"203","author":"Adroher","year":"2015","journal-title":"Int. J. Food Microbiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1016\/S0020-7519(99)00178-2","article-title":"Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: Ascaridoidea) defined by polymerase chain reaction-based restriction fragment length polymorphism","volume":"30","author":"Mathiopoulos","year":"2000","journal-title":"Int. J. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1430","DOI":"10.1645\/GE-565R1.1","article-title":"Molecular characterization of larval anisakid nematodes from marine fishes of Madeira by a PCR-based approach, with evidence for a new species","volume":"91","author":"Pontes","year":"2005","journal-title":"J. Parasitol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1645\/17-115","article-title":"Hysterothylacium fabri (Nematoda: Raphidascarididae) in Mullus surmuletus (Perciformes: Mullidae) and Uranoscopus scaber (Perciformes: Uranoscopidae) from the Mediterranean","volume":"104","author":"Tedesco","year":"2018","journal-title":"J. Parasitol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1016\/S0014-4894(02)00031-0","article-title":"Ascaridoidea: A simple DNA assay for identification of 11 species infecting marine and freshwater fish, mammals, and fish-eating birds","volume":"101","author":"Kijewska","year":"2002","journal-title":"Exp. Parasitol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1016\/j.ijpara.2019.04.004","article-title":"Drivers of parasite community structure in fishes of the continental shelf of the Western Mediterranean: The importance of host phylogeny and autecological traits","volume":"49","author":"Cartes","year":"2019","journal-title":"Int. J. Parasitol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"575","DOI":"10.2307\/3284227","article-title":"Parasitology meets ecology on its own terms: Margolis et al. revisited","volume":"83","author":"Bush","year":"1997","journal-title":"J. Parasitol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1016\/j.pt.2019.01.003","article-title":"Biostatistics for parasitologists\u2014A primer to Quantitative Parasitology","volume":"35","author":"Reiczigel","year":"2019","journal-title":"Trends Parasitol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Morales-Yuste, M., S\u00e1nchez-Yebra, W., Garrido, M., Ben\u00edtez, R., and Adroher, F.J. (2022). Anisakis infection in the spotted flounder Citharus linguatula (Pleuronectiformes: Citharidae) caught in the Gulf of Cadiz (Area FAO 27-ICES IXa) appears to negatively affect fish growth. Pathogens, 11.","DOI":"10.3390\/pathogens11121432"},{"key":"ref_30","first-page":"285","article-title":"Caracter\u00edsticas de la infestaci\u00f3n de la faneca, Trisopterus luscus (L.), en las costas de Asturias","volume":"9","author":"Villegas","year":"1993","journal-title":"Bolet\u00edn. Inst. Espa\u00f1ol Oceanogr."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1016\/j.actatropica.2019.04.003","article-title":"Differential proteolytic activity in Anisakis simplex s.s. and Anisakis pegreffii, two sibling species from the complex Anisakis simplex s.l., major etiological agents of anisakiasis","volume":"195","author":"Adroher","year":"2019","journal-title":"Acta Trop."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"744","DOI":"10.1089\/fpd.2019.2633","article-title":"Differential cleaving of specific substrates for cathepsin-like activity shows cysteine and serine protease activities and a differential profile between Anisakis simplex s.s. and Anisakis pegreffii, sibling species major etiologic agents of anisakiasis","volume":"16","author":"Adroher","year":"2019","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1089\/fpd.2011.1076","article-title":"Anisakis simplex sensu stricto and Anisakis pegreffii: Biological characteristics and pathogenetic potential in human anisakiasis","volume":"9","author":"Arizono","year":"2012","journal-title":"Foodborne Pathog. Dis."},{"key":"ref_34","unstructured":"(2004). EU Regulation EC 853\/2004 of the European Parliament and of the Council of 29 April 2004 laying down specific hygiene rules of foodstuffs. Off. J. Eur. Union, L 139, 55."},{"key":"#cr-split#-ref_35.1","unstructured":"(2005). EU Commission Regulation"},{"key":"#cr-split#-ref_35.2","unstructured":"(EC) No 2074\/2005 of 5 December 2005. Off. J. Eur. Union, L338, 27-59."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1016\/S0091-6749(98)70364-1","article-title":"Allergenic cross-reactivity between third stage larvae of Hysterothylacium aduncum and Anisakis simplex","volume":"101","author":"Quirce","year":"1998","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1141","DOI":"10.4315\/0362-028X-63.8.1141","article-title":"Anisakid larvae in the musculature of the Argentinean hake, Merluccius hubbsi","volume":"63","author":"Herreras","year":"2000","journal-title":"J. Food Prot."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Ahuir-Baraja, A.E., Llobat, L., and Garijo, M.M. (2021). Effectiveness of gutting blue whiting (Micromesistius poutassou, Risso, 1827), in Spanish supermarkets as an anisakidosis safety measure. Foods, 10.","DOI":"10.3390\/foods10040862"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"100937","DOI":"10.1016\/j.ijppaw.2024.100937","article-title":"Location and elimination of Anisakis simplex third stage larvae in Atlantic herring Clupea harengus L","volume":"24","author":"Kumas","year":"2024","journal-title":"Int. J. Parasitol. Parasites Wildl."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1942","DOI":"10.1017\/S0031182022001305","article-title":"Anisakid parasites (Nematoda: Anisakidae) in 3 commercially important gadid fish species from the southern Barents Sea, with emphasis on key infection drivers and spatial distribution within the hosts","volume":"149","author":"Levsen","year":"2022","journal-title":"Parasitology"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"EFSA-BIOHAZ Panel, Koutsoumanis, K., Allende, A., \u00c1lvarez-Ord\u00f3\u00f1ez, A., Bover-Cid, S., Chemaly, M., De Cesare, A., Herman, L., Hilbert, F., and Lindqvist, R. (2024). Re-evaluation of certain aspects of the EFSA Scientific Opinion of April 2010 on risk assessment of parasites in fishery products, based on new scientific data. Part 1: ToRs1\u20133. EFSA J., 22, e8719.","DOI":"10.2903\/j.efsa.2024.8719"},{"key":"ref_42","unstructured":"Pascual, S., Gonz\u00e1lez, \u00c1.F., Vello, C., and Outeiri\u00f1o, L. (2010, January 25). Higienizaci\u00f3n de par\u00e1sitos en subproductos de la pesca extractiva. Proceedings of the Simposio Internacional Estrategias de Gesti\u00f3n de Parasitosis en Productos Pesqueros, Vigo, Spain."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1007\/s00436-019-06339-1","article-title":"How effective is freezing at killing Anisakis simplex, Pseudoterranova krabbei, and P. decipiens larvae? An experimental evaluation of time-temperature conditions","volume":"118","author":"Podolska","year":"2019","journal-title":"Parasitol. Res."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/3\/252\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:46:50Z","timestamp":1760028410000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/3\/252"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,4]]},"references-count":44,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["pathogens14030252"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14030252","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,4]]}}}