{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T23:52:03Z","timestamp":1770421923297,"version":"3.49.0"},"reference-count":44,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T00:00:00Z","timestamp":1741651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Bulgarian National Science Fund","award":["KP-06-H-43\/2\/27.11.2020"],"award-info":[{"award-number":["KP-06-H-43\/2\/27.11.2020"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The specificity of the life cycle of T. gondii, the causative agent of toxoplasmosis, determines its relevance in some patient groups; especially in women of childbearing age. The objective of this study was to ascertain the prevalence of this parasitic infection in Bulgaria between 2014 and 2023, focusing on pregnant women and those applying for an in vitro procedure or after an abortion. During the period, 115,053 individuals were tested for toxoplasmosis in the country, with an average seropositivity rate of 16.96%. At the NRL for the Diagnosis of Parasitic Diseases, ELISA tests were utilized to ascertain specific Toxoplasma IgG, IgM, and IgA antibodies, IgG avidity, and PCR to substantiate the DNA of the parasite. Between 2014 and 2023, the laboratory conducted tests on 631 pregnant women, and 161 women applying for in vitro fertilization or after an abortion. In 24.7% of pregnant women, data demonstrated the presence of IgG antibodies only, and Toxoplasma IgG and IgM or IgG, IgM, and IgA antibodies were found in 16.6% and 8% of women, respectively. In the subset of individuals examined after an abortion or for an in vitro procedure, IgG was detected in 28%, IgG and IgM in 13%, and IgG, IgM, and IgA antibodies in 3.7%. Seroprevalence rises with increasing age, but does not show any dynamic compared to our previous studies. Our results show a higher seropositivity for toxoplasmosis in pregnant women in Bulgaria than in neighboring countries.<\/jats:p>","DOI":"10.3390\/pathogens14030270","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T10:53:07Z","timestamp":1741690387000},"page":"270","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["The Prevalence of Toxoplasmosis in Bulgaria for the Period 2014\u20132023, with a Focus on Pregnant Women"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7530-9131","authenticated-orcid":false,"given":"Iskra","family":"Rainova","sequence":"first","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, National Centre of Infectious and Parasitic Diseases (NCIPD), 26 Yanko Sakazov Blvd., 1504 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2745-3344","authenticated-orcid":false,"given":"Rumen","family":"Harizanov","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, National Centre of Infectious and Parasitic Diseases (NCIPD), 26 Yanko Sakazov Blvd., 1504 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mihaela","family":"Videnova","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, National Centre of Infectious and Parasitic Diseases (NCIPD), 26 Yanko Sakazov Blvd., 1504 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4638-9379","authenticated-orcid":false,"given":"Nina","family":"Tsvetkova","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, National Centre of Infectious and Parasitic Diseases (NCIPD), 26 Yanko Sakazov Blvd., 1504 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raina","family":"Borisova","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, National Centre of Infectious and Parasitic Diseases (NCIPD), 26 Yanko Sakazov Blvd., 1504 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eleonora","family":"Kaneva","sequence":"additional","affiliation":[{"name":"Department of Parasitology and Tropical Medicine, National Centre of Infectious and Parasitic Diseases (NCIPD), 26 Yanko Sakazov Blvd., 1504 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yana","family":"Todorova","sequence":"additional","affiliation":[{"name":"Department of Immunology, National Centre of Infectious and Parasitic Diseases (NCIPD), 26 Yanko Sakazov Blvd., 1504 Sofia, Bulgaria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,11]]},"reference":[{"key":"ref_1","unstructured":"Kurdova, R., and Petrov, P. 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