{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T14:30:18Z","timestamp":1789223418752,"version":"build-2803163510"},"reference-count":65,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2025,3,16]],"date-time":"2025-03-16T00:00:00Z","timestamp":1742083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Institute of General Medical Sciences of the National Institutes of Health","award":["5P20GM103427"],"award-info":[{"award-number":["5P20GM103427"]}]},{"name":"University of Nebraska at Omaha FUSE and GRACA programs","award":["5P20GM103427"],"award-info":[{"award-number":["5P20GM103427"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>The rise of bacterial infections is a global health issue that calls for the development and availability of additional antimicrobial agents. Known for its in vitro effects on Gram-positive organisms, the drug-like small molecule marinopyrrole A was re-examined for the potential of broader efficacy against a wider array of microbes. We uncovered selective efficacy against an important subset of Gram-negative bacteria from three genera: Neisseria, Moraxella, and Campylobacter. This susceptibility is correlated with the absence of canonical LPS in these specific Gram-negative species, a phenomenon observed with other hydrophobic anti-microbial compounds. Further, when exposed to molecules which inhibit the LpxC enzyme of the LPS synthesis pathway, previously resistant LPS-producing Gram-negative bacteria showed increased susceptibility to marinopyrrole A. These results demonstrate marinopyrrole A\u2019s efficacy against a broader range of Gram-negative bacteria than previously known, including N. gonorrhea, a species identified as a priority pathogen by the WHO.<\/jats:p>","DOI":"10.3390\/pathogens14030290","type":"journal-article","created":{"date-parts":[[2025,3,17]],"date-time":"2025-03-17T07:49:57Z","timestamp":1742197797000},"page":"290","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Expanded Gram-Negative Activity of Marinopyrrole A"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-8193-9732","authenticated-orcid":false,"given":"Clare F.","family":"Euteneuer","sequence":"first","affiliation":[{"name":"Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3230-4378","authenticated-orcid":false,"given":"Brianna N.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7938-9252","authenticated-orcid":false,"given":"LeeAnna M.","family":"Lui","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6237-9640","authenticated-orcid":false,"given":"Andrew J.","family":"Neville","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0526-3841","authenticated-orcid":false,"given":"Paul H.","family":"Davis","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Nebraska at Omaha, Omaha, NE 68182, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,16]]},"reference":[{"key":"ref_1","unstructured":"(2022). Global mortality associated with 33 bacterial pathogens in 2019: A systematic analysis for the Global Burden of Disease Study 2019. Lancet, 400, 2221\u20132248."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"231","DOI":"10.1080\/10408410701647560","article-title":"Ecological origins of novel human pathogens","volume":"33","author":"Woolhouse","year":"2007","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1016\/j.bcp.2017.01.003","article-title":"What is an \u201cideal\u201d antibiotic? Discovery challenges and path forward","volume":"133","author":"Singh","year":"2017","journal-title":"Biochem. Pharmacol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"45","DOI":"10.18773\/austprescr.2020.008","article-title":"Oral or intravenous antibiotics?","volume":"43","author":"McCarthy","year":"2020","journal-title":"Aust. Prescr."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1021\/ol702952n","article-title":"The marinopyrroles, antibiotics of an unprecedented structure class from a marine Streptomyces sp.","volume":"10","author":"Hughes","year":"2008","journal-title":"Org. Lett."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5195","DOI":"10.1074\/jbc.M201988200","article-title":"Tankyrase 1 interacts with Mcl-1 proteins and inhibits their regulation of apoptosis","volume":"278","author":"Bae","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Pandey, M.K., Gowda, K., Doi, K., Sharma, A.K., Wang, H.-G., and Amin, S. (2013). Proteasomal Degradation of Mcl-1 by Maritoclax Induces Apoptosis and Enhances the Efficacy of ABT-737 in Melanoma Cells. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0078570"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"10224","DOI":"10.1074\/jbc.M111.334532","article-title":"Discovery of Marinopyrrole A (Maritoclax) as a Selective Mcl-1 Antagonist that Overcomes ABT-737 Resistance by Binding to and Targeting Mcl-1 for Proteasomal Degradation","volume":"287","author":"Doi","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1335","DOI":"10.3390\/md12031335","article-title":"Cyclic marinopyrrole derivatives as disruptors of Mcl-1 and Bcl-x(L) binding to Bim","volume":"12","author":"Cheng","year":"2014","journal-title":"Mar. Drugs"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e880","DOI":"10.1038\/cddis.2013.411","article-title":"Purported Mcl-1 inhibitor marinopyrrole A fails to show selective cytotoxicity for Mcl-1-dependent cell lines","volume":"4","author":"Eichhorn","year":"2013","journal-title":"Cell Death Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2041","DOI":"10.1016\/j.tetlet.2010.09.059","article-title":"Total synthesis and biological evaluation of marinopyrrole A and analogues","volume":"52","author":"Nicolaou","year":"2011","journal-title":"Tetrahedron Lett."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1077","DOI":"10.4161\/cbt.29186","article-title":"Maritoclax induces apoptosis in acute myeloid leukemia cells with elevated Mcl-1 expression","volume":"15","author":"Doi","year":"2014","journal-title":"Cancer Biol. Ther."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"3305","DOI":"10.1128\/AAC.01211-10","article-title":"Pharmacological Properties of the Marine Natural Product Marinopyrrole A against Methicillin-Resistant Staphylococcus aureus \u25bf","volume":"55","author":"Haste","year":"2011","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2927","DOI":"10.3390\/md11082927","article-title":"Marinopyrrole Derivatives as Potential Antibiotic Agents against Methicillin-Resistant Staphylococcus aureus (II)","volume":"11","author":"Cheng","year":"2013","journal-title":"Mar. Drugs"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Breijyeh, Z., Jubeh, B., and Karaman, R. (2020). Resistance of Gram-Negative Bacteria to Current Antibacterial Agents and Approaches to Resolve It. Molecules, 25.","DOI":"10.3390\/molecules25061340"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Mai-Prochnow, A., Clauson, M., Hong, J., and Murphy, A.B. (2016). Gram positive and Gram negative bacteria differ in their sensitivity to cold plasma. Sci. Rep., 6.","DOI":"10.1038\/srep38610"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5158","DOI":"10.1021\/acs.chemrev.0c01213","article-title":"How to Enter a Bacterium: Bacterial Porins and the Permeation of Antibiotics","volume":"121","author":"Prajapati","year":"2021","journal-title":"Chem. Rev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/ecosalplus.esp-0001-2018","article-title":"Function and biogenesis of lipopolysaccharides","volume":"8","author":"Bertani","year":"2018","journal-title":"EcoSal Plus"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Benthien, H., Fresenborg, B., P\u00e4tzold, L., Elhawy, M.I., Huc-Brandt, S., Beisswenger, C., Krasteva-Christ, G., Becker, S.L., Molle, V., and Knobloch, J.K. (2022). The Transcription Factor SpoVG Is of Major Importance for Biofilm Formation of Staphylococcus epidermidis under In Vitro Conditions, but Dispensable for In Vivo Biofilm Formation. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms23063255"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1111\/j.1469-0691.1998.tb00393.x","article-title":"Psychotropic drugs inhibit swarming in Proteus spp. and related genera","volume":"4","year":"1998","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Rice, J., Gibson, J., Young, E., Souder, K., Cunningham, K., and Schmitt, D.M. (2024). Low Oxygen Concentration Reduces Neisseria gonorrhoeae Susceptibility to Resazurin. Antibiotics, 13.","DOI":"10.3390\/antibiotics13050395"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/978-1-61779-346-2_1","article-title":"Neisseria meningitidis: Biology, Microbiology, and Epidemiology","volume":"799","author":"Rouphael","year":"2012","journal-title":"Methods Mol. Biol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1016\/S0168-1605(03)00160-0","article-title":"Attachment of Escherichia coli O157:H7 grown in tryptic soy broth and nutrient broth to apple and lettuce surfaces as related to cell hydrophobicity, surface charge, and capsule production","volume":"96","author":"Hassan","year":"2004","journal-title":"Int. J. Food Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Li, B., Zhan, M., Evivie, S.E., Jin, D., Zhao, L., Chowdhury, S., Sarker, S.K., Huo, G., and Liu, F. (2018). Evaluating the Safety of Potential Probiotic Enterococcus durans KLDS6.0930 Using Whole Genome Sequencing and Oral Toxicity Study. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01943"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"326","DOI":"10.1111\/j.1745-4565.2007.00083.x","article-title":"In vitro survival at low PH and acid adaptation response of Campylobacter jejuni and Campylobacter coli","volume":"27","author":"Shaheen","year":"2007","journal-title":"J. Food Saf."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Tan, A., Blakeway, L.V., Yang, Y., Zhou, Y., Atack, J.M., Peak, I.R., and Seib, K.L. (2020). Moraxella catarrhalis phase-variable loci show differences in expression during conditions relevant to disease. PLoS ONE, 15.","DOI":"10.1371\/journal.pone.0234306"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1385\/1-59259-321-6:51","article-title":"General Methods for Culturing Haemophilus influenzae","volume":"Volume 71","author":"Poje","year":"2002","journal-title":"Hemophilus Influenzae Protocols"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1007\/978-1-4939-9929-3_23","article-title":"Determination of ADC Cytotoxicity in Immortalized Human Cell Lines","volume":"2078","author":"Wu","year":"2020","journal-title":"Methods Mol. Biol."},{"key":"ref_29","unstructured":"(2025, March 06). Calculate ECanything from EC50. Available online: https:\/\/www.graphpad.com\/quickcalcs\/ecanything1\/."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3183","DOI":"10.1093\/jac\/dkab328","article-title":"Rapid high-resolution detection of colistin resistance in Gram-negative bacteria using flow cytometry: A comparison with broth microdilution, a commercial screening test and WGS","volume":"76","author":"Ekelund","year":"2021","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1021\/jm2014748","article-title":"Potent inhibitors of LpxC for the treatment of Gram-negative infections","volume":"55","author":"Brown","year":"2012","journal-title":"J. Med. Chem."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2064","DOI":"10.1093\/jac\/dky151","article-title":"Treatment of human challenge and MDR strains of Neisseria gonorrhoeae with LpxC inhibitors","volume":"73","author":"John","year":"2018","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1021\/acschembio.3c00773","article-title":"Antibacterial Marinopyrroles and Pseudilins Act as Protonophores","volume":"19","author":"Straetener","year":"2024","journal-title":"ACS Chem. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e00794-21","DOI":"10.1128\/AAC.00794-21","article-title":"Analogs of Marinopyrrole A Show Enhancement to Observed In Vitro Potency against Acute Toxoplasma gondii Infection","volume":"66","author":"Martens","year":"2022","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1016\/j.ejpb.2022.10.006","article-title":"Lipopolysaccharide Structure and the Phenomenon of Low Endotoxin Recovery","volume":"180","author":"Gorman","year":"2022","journal-title":"Eur. J. Pharm. Biopharm."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"139","DOI":"10.3109\/10408419609106458","article-title":"The lipooligosaccharides of pathogenic gram-negative bacteria","volume":"22","author":"Preston","year":"1996","journal-title":"Crit. Rev. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"7569","DOI":"10.1128\/IAI.73.11.7569-7577.2005","article-title":"Moraxella catarrhalis Bacterium without Endotoxin, a Potential Vaccine Candidate","volume":"73","author":"Peng","year":"2005","journal-title":"Infect. Immun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1093\/femsre\/fuw007","article-title":"Lipopolysaccharide modification in Gram-negative bacteria during chronic infection","volume":"40","author":"Maldonado","year":"2016","journal-title":"FEMS Microbiol. Rev."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"ofad378","DOI":"10.1093\/ofid\/ofad378","article-title":"Epidemiology and Antimicrobial Resistance of Campylobacter Infections in the United States, 2005\u20132018","volume":"10","author":"Ford","year":"2023","journal-title":"Open Forum Infect Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1128\/CMR.00055-07","article-title":"Host-pathogen interactions in Campylobacter infections: The host perspective","volume":"21","author":"Janssen","year":"2008","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Finsterer, J. (2022). Triggers of Guillain-Barr\u00e9 Syndrome: Campylobacter jejuni Predominates. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms232214222"},{"key":"ref_42","first-page":"mgen000875","article-title":"Emerging patterns of fluoroquinolone resistance in Campylobacter jejuni in the UK [1998\u20132018]","volume":"8","author":"Veltcheva","year":"2022","journal-title":"Microb. Genom."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Othman, D., Elhosseiny, N.M., Eltayeb, W.N., and Attia, A.S. (2024). The Moraxella catarrhalis AdhC-FghA system is important for formaldehyde detoxification and protection against pulmonary clearance. Med. Microbiol. Immunol., 213.","DOI":"10.1007\/s00430-024-00785-0"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Alexandrova, A.S., Boyanov, V.S., Mihova, K.Y., and Gergova, R.T. (2024). Phylogenetic Lineages and Diseases Associated with Moraxella catarrhalis Isolates Recovered from Bulgarian Patients. Int. J. Mol. Sci., 25.","DOI":"10.3390\/ijms25189769"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1084\/jem.191.6.949","article-title":"Role of Lipooligosaccharide in Opa-Independent Invasion of Neisseria gonorrhoeae into Human Epithelial Cells","volume":"191","author":"Song","year":"2000","journal-title":"J. Exp. Med."},{"key":"ref_46","unstructured":"(2024, August 15). WHO Bacterial Priority Pathogens List, 2024: Bacterial Pathogens of Public Health Importance to Guide Research, Development and Strategies to Prevent and Control Antimicrobial Resistance. Available online: https:\/\/www.who.int\/publications\/i\/item\/9789240093461."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Melendez, J.H., Edwards, V.L., Muniz Tirado, A., Hardick, J., Mehta, A., Aluvathingal, J., D\u2019Mello, A., Gaydos, C.A., Manabe, Y.C., and Tettelin, H. (2024). Local emergence and global evolution of Neisseria gonorrhoeae with high-level resistance to azithromycin. Antimicrob. Agents Chemother., e0092724.","DOI":"10.1128\/aac.00927-24"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Walker, E., van Niekerk, S., Hanning, K., Kelton, W., and Hicks, J. (2023). Mechanisms of host manipulation by Neisseria gonorrhoeae. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1119834"},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Hooshiar, M.H., Sholeh, M., Beig, M., Azizian, K., and Kouhsari, E. (2024). Global trends of antimicrobial resistance rates in Neisseria gonorrhoeae: A systematic review and meta-analysis. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1284665"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Unemo, M., Del Rio, C., and Shafer, W.M. (2016). Antimicrobial Resistance Expressed by Neisseria gonorrhoeae: A Major Global Public Health Problem in the 21st Century. Microbiol. Spectr., 4.","DOI":"10.1128\/microbiolspec.EI10-0009-2015"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Willerton, L., Lucidarme, J., Walker, A., Lekshmi, A., Clark, S.A., Walsh, L., Bai, X., Lee-Jones, L., and Borrow, R. (2021). Antibiotic resistance among invasive Neisseria meningitidis isolates in England, Wales and Northern Ireland (2010\/11 to 2018\/19). PLoS ONE, 16.","DOI":"10.1371\/journal.pone.0260677"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Mikucki, A., McCluskey, N.R., and Kahler, C.M. (2022). The Host-Pathogen Interactions and Epicellular Lifestyle of Neisseria meningitidis. Front. Cell Infect. Microbiol., 12.","DOI":"10.3389\/fcimb.2022.862935"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Mikucki, A., and Kahler, C.M. (2023). Microevolution and Its Impact on Hypervirulence, Antimicrobial Resistance, and Vaccine Escape in Neisseria meningitidis. Microorganisms, 11.","DOI":"10.3390\/microorganisms11123005"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"a025304","DOI":"10.1101\/cshperspect.a025304","article-title":"Antibacterial Drug Discovery Targeting the Lipopolysaccharide Biosynthetic Enzyme LpxC","volume":"6","author":"Erwin","year":"2016","journal-title":"Cold Spring Harb. Perspect. Med."},{"key":"ref_55","doi-asserted-by":"crossref","unstructured":"M\u00f6ller, A.-M., V\u00e1zquez-Hern\u00e1ndez, M., Kutscher, B., Brysch, R., Br\u00fcckner, S., Marino, E.C., Kleetz, J., Senges, C.H.R., Sch\u00e4kermann, S., and Bandow, J.E. (2024). Common and varied molecular responses of Escherichia coli to five different inhibitors of the lipopolysaccharide biosynthetic enzyme LpxC. J. Biol. Chem., 300.","DOI":"10.1016\/j.jbc.2024.107143"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"3485","DOI":"10.1021\/acs.langmuir.6b00240","article-title":"The Effect of Lipopolysaccharide Core Oligosaccharide Size on the Electrostatic Binding of Antimicrobial Proteins to Models of the Gram Negative Bacterial Outer Membrane","volume":"32","author":"Clifton","year":"2016","journal-title":"Langmuir"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"e01205","DOI":"10.1128\/mbio.01205-23","article-title":"The Gram-negative permeability barrier: Tipping the balance of the in and the out","volume":"14","author":"Maher","year":"2023","journal-title":"mBio"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"S412","DOI":"10.1093\/clinids\/5.Supplement_3.S412","article-title":"Rifampin: Spectrum of antibacterial activity","volume":"5","author":"Thornsberry","year":"1983","journal-title":"Rev. Infect. Dis."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1007\/s101560050034","article-title":"Diffusion of macrolide antibiotics through the outer membrane of Moraxella catarrhalis","volume":"5","author":"Tsujimoto","year":"1999","journal-title":"J. Infect. Chemother."},{"key":"ref_60","unstructured":"HMDB (2024, June 26). Showing Metabocard for (-)-Marinopyrrole A (HMDB0242215). Available online: https:\/\/hmdb.ca\/metabolites\/HMDB0242215."},{"key":"ref_61","doi-asserted-by":"crossref","unstructured":"Schaeffer, J., Lippert, K., Pleininger, S., St\u00f6ger, A., Hasenberger, P., Stadlbauer, S., Heger, F., Eigentler, A., Geusau, A., and Indra, A. (2022). Rifampicin Resistance Associated with rpoB Mutations in Neisseria gonorrhoeae Clinical Strains Isolated in Austria, 2016 to 2020. Microbiol Spectr, 10.","DOI":"10.1128\/spectrum.02757-21"},{"key":"ref_62","unstructured":"(2025, February 23). PubChem Rifampin, Available online: https:\/\/pubchem.ncbi.nlm.nih.gov\/compound\/135398735."},{"key":"ref_63","doi-asserted-by":"crossref","unstructured":"Qu, X., Zhou, J., Huang, H., Wang, W., Xiao, Y., Tang, B., Liu, H., Xu, C., and Xiao, X. (2022). Genomic Investigation of Proteus mirabilis Isolates Recovered from Pig Farms in Zhejiang Province, China. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.952982"},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"e01243-18","DOI":"10.1128\/AAC.01243-18","article-title":"Evolution of Rifampin Resistance in Escherichia coli and Mycobacterium smegmatis Due to Substandard Drugs","volume":"63","author":"Weinstein","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e01450-19","DOI":"10.1128\/AAC.01450-19","article-title":"Pyrrolomycins Are Potent Natural Protonophores","volume":"63","author":"Valderrama","year":"2019","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/3\/290\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T16:54:32Z","timestamp":1760028872000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/3\/290"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,16]]},"references-count":65,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3]]}},"alternative-id":["pathogens14030290"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14030290","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,16]]}}}