{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T12:21:15Z","timestamp":1771503675453,"version":"3.50.1"},"reference-count":37,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Although quilombola individuals and their dogs may be exposed to hemoparasites such as A. phagocytophilum, B. microti, and E. chaffeensis, no study to date has been conducted in these populations. The aim of this study was to investigate the presence of antibodies against Anaplasma phagocytophilum, Babesia microti, and Ehrlichia chaffeensis in humans and dogs from quilombola communities in Brazil. Serum samples from humans and dogs were collected from four rural quilombola communities and analyzed using indirect immunofluorescence assays. The results revealed antibody levels of 8% for A. phagocytophilum, 3% for B. microti, and 1% for E. chaffeensis in humans and 60%, 50%, and 65%, respectively, in dogs. Notably, women were significantly more likely to be seropositive for A. phagocytophilum than men (p = 0.0289). Dogs from the Serra do Apon community more commonly had A. phagocytophilum (p = 0.0477) and B. microti (p = 0.0448) than those from the other areas. To the best of our knowledge, this is the first study to report human exposure to A. phagocytophilum and the ocurrence of B. microti in Brazil. The antibody level of vector-borne diseases in humans is a public health concern, particularly in vulnerable populations and rural areas. The dogs were universally hosted Rhipicephalus sanguineus ticks, suggesting their possible role in transmission. Thus, further epidemiological surveillance studies should be conducted in vulnerable populations to mitigate the impact of such zoonotic diseases.<\/jats:p>","DOI":"10.3390\/pathogens14040318","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T04:17:14Z","timestamp":1742962634000},"page":"318","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Serosurvey of Anaplasma phagocytophilum, Babesia microti, and Ehrlichia chaffeensis in Quilombola Communities of Southern Brazil"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1178-5643","authenticated-orcid":false,"given":"Danilo Alves de","family":"Fran\u00e7a","sequence":"first","affiliation":[{"name":"Department of Animal Production and Preventive Veterinary Medicine, School of Veterinary Medicine and Animals Science, S\u00e3o Paulo State University, Botucatu 18618-687, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6579-1278","authenticated-orcid":false,"given":"Louise Bach","family":"Kmetiuk","sequence":"additional","affiliation":[{"name":"Zoonosis Surveillance Unit, City Secretary of Health, Curitiba 81265-320, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filipe Pereira da","family":"Silva","sequence":"additional","affiliation":[{"name":"Service of Virology and Rickettsiosis, Octavio Magalhaes Institute, Belo Horizonte 30510-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni Kalempa","family":"Panazzolo","sequence":"additional","affiliation":[{"name":"Graduate College of Pharmaceutical Sciences, State University of Ponta Grossa, Ponta Grossa 84030-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7554-1520","authenticated-orcid":false,"given":"Leandro Meneguelli","family":"Biondo","sequence":"additional","affiliation":[{"name":"Interdisciplinary Graduate Studies, University of British Columbia, Kelowna, BC V6T 1Z4, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Orlei Jos\u00e9","family":"Domingues","sequence":"additional","affiliation":[{"name":"Graduate College of Pharmaceutical Sciences, State University of Ponta Grossa, Ponta Grossa 84030-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1946-3262","authenticated-orcid":false,"given":"Giovani Marino","family":"F\u00e1vero","sequence":"additional","affiliation":[{"name":"Graduate College of Pharmaceutical Sciences, State University of Ponta Grossa, Ponta Grossa 84030-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2480-9042","authenticated-orcid":false,"given":"Ana \u00cdris de Lima","family":"Dur\u00e9","sequence":"additional","affiliation":[{"name":"Service of Virology and Rickettsiosis, Octavio Magalhaes Institute, Belo Horizonte 30510-010, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4182-5821","authenticated-orcid":false,"given":"Alexander Welker","family":"Biondo","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Federal University of Paran\u00e1, Curitiba 80060-240, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1590\/S1414-49802010000100016","article-title":"Ethnic identity and power: Quilombos in Brazilian public policy","volume":"13","author":"Calheiros","year":"2010","journal-title":"Rev. Kat\u00e1lysis"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Martins, M.F.d.S., Pereira, M.B., Ferreira, J.d.J.G., Fran\u00e7a, A.d.O., Cominetti, M.C., Ferreira, E.d.C., Dorval, M.E.M.C., Rossi, C.L., Mazon, S.d.B., and de Almeida, E.A. (2018). Serological and molecular inquiry of Chagas Disease in an Afro-descendant settlement in Mato Grosso Do Sul State, Brazil. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0189448"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e200173","DOI":"10.1590\/1678-9865202134e200173","article-title":"Social Inequalities and household food insecurity in Quilombola communities in Brazil","volume":"34","author":"Cherol","year":"2021","journal-title":"Rev. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"de la Fuente, J., Estrada-Pe\u00f1a, A., Rafael, M., Almaz\u00e1n, C., Berm\u00fadez, S., Abdelbaset, A.E., Kasaija, P.D., Kabi, F., Akande, F.A., and Ajagbe, D.O. (2023). Perception of Ticks and Tick-Borne Diseases Worldwide. Pathogens, 12.","DOI":"10.3390\/pathogens12101258"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1767","DOI":"10.1001\/jama.2016.2884","article-title":"Diagnosis, treatment, and prevention of Lyme disease, human granulocytic anaplasmosis, and babesiosis: A Review","volume":"315","author":"Sanchez","year":"2016","journal-title":"JAMA"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.actatropica.2010.09.006","article-title":"Genetic analysis of ticks belonging to the Rhipicephalus Sanguineus group in Latin America","volume":"117","author":"Marcili","year":"2011","journal-title":"Acta Trop."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.ttbdis.2016.09.019","article-title":"Multiple Resistance to acaricides in field populations of Rhipicephalus Microplus from Rio Grande Do Sul State, Southern Brazil","volume":"8","author":"Klafke","year":"2017","journal-title":"Ticks Tick. Borne Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/S0304-4017(01)00649-5","article-title":"Seasonal Dynamics of Ticks (Acari: Ixodidae) on Horses in the State of S\u00e3o Paulo, Brazil","volume":"105","author":"Labruna","year":"2002","journal-title":"Vet. Parasitol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"101967","DOI":"10.1016\/j.ttbdis.2022.101967","article-title":"Prevalence of Bovine babesia spp., Anaplasma Marginale, and Their Co-Infections in Latin America: Systematic Review-Meta-Analysis","volume":"13","author":"Ferreira","year":"2022","journal-title":"Ticks Tick. Borne Dis."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"540","DOI":"10.3201\/eid2903.220320","article-title":"Associations of Anaplasma phagocytophilum bacteria variants in Ixodes scapularis ticks and humans, New York, USA","volume":"29","author":"Prusinski","year":"2023","journal-title":"Emerg. Infect. Dis."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1128\/CMR.16.1.37-64.2003","article-title":"Ehrlichia chaffeensis: A prototypical emerging pathogen","volume":"16","author":"Paddock","year":"2003","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/j.idc.2022.02.009","article-title":"Human Babesiosis","volume":"36","author":"Waked","year":"2022","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.4103\/0972-9062.364756","article-title":"Global status of Anaplasma phagocytophilum infections in human population: A 50-year (1970\u20132020) Meta-Analysis","volume":"60","author":"Karshima","year":"2023","journal-title":"J. Vector Borne Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1186\/1756-3305-6-348","article-title":"Molecular epidemiology of the emerging zoonosis agent Anaplasma phagocytophilum (Foggie, 1949) in dogs and ixodid ticks in Brazil","volume":"6","author":"Santos","year":"2013","journal-title":"Parasit Vectors"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"100055","DOI":"10.1016\/j.vas.2019.100055","article-title":"Molecular evidence of Anaplasma phagocytophilum and Theileria equi coinfection in horses from Rio de Janeiro, Brazil","volume":"7","author":"Roier","year":"2019","journal-title":"Vet. Anim. Sci."},{"key":"ref_16","first-page":"227","article-title":"A case of imported human babesiosis in Poland","volume":"43","author":"Humiczewska","year":"1997","journal-title":"Wiad. Parazytol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1590\/S1984-29612011000100002","article-title":"Ehrlichiosis in Brazil","volume":"20","author":"Vieira","year":"2011","journal-title":"Rev. Bras. Parasitol. Vet."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.vetpar.2006.02.038","article-title":"Detection of Ehrlichia chaffeensis in Brazilian marsh deer (Blastocerus dichotomus)","volume":"139","author":"Machado","year":"2006","journal-title":"Vet. Parasitol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1590\/S1413-86702006000100002","article-title":"More about Human Monocytotropic Ehrlichiosis in Brazil: Serological evidence of nine new cases","volume":"10","author":"Valle","year":"2006","journal-title":"Braz. J. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"131","DOI":"10.26895\/geosaberes.v8i15.576","article-title":"Perman\u00eancia e resist\u00eancia das comunidades remanescentes de Quilombos no Paran\u00e1","volume":"8","author":"Campos","year":"2017","journal-title":"GEOSABERES Rev. Estud. Geoeducacionais"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2000","DOI":"10.1093\/ije\/dyac123","article-title":"Sample Size Calculators for Planning Stepped-Wedge Cluster Randomized Trials: A Review and Comparison","volume":"51","author":"Ouyang","year":"2022","journal-title":"Int. J. Epidemiol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.vetpar.2018.03.001","article-title":"Antiprotozoal treatment of canine babesiosis","volume":"254","author":"Baneth","year":"2018","journal-title":"Vet. Parasitol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/j.tvjl.2019.01.015","article-title":"An Update on the treatment of canine monocytic ehrlichiosis (Ehrlichia canis)","volume":"246","author":"Mylonakis","year":"2019","journal-title":"Vet. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1016\/j.cvsm.2022.06.011","article-title":"Hepatozoonosis of dogs and cats","volume":"52","author":"Baneth","year":"2022","journal-title":"Vet. Clin. N. Am. Small Anim. Pract."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"993","DOI":"10.1016\/j.pt.2022.08.011","article-title":"Rhipicephalus sanguineus (brown dog tick)","volume":"38","author":"Otranto","year":"2022","journal-title":"Trends Parasitol."},{"key":"ref_26","first-page":"137","article-title":"Canine ehrlichiosis","volume":"10","author":"Skotarczak","year":"2003","journal-title":"Ann. Agric. Environ. Med."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Aziz, M.U., Hussain, S., Song, B., Ghauri, H.N., Zeb, J., and Sparagano, O.A. (2022). Ehrlichiosis in Dogs: A Comprehensive Review about the Pathogen and Its Vectors with Emphasis on South and East Asian Countries. Vet. Sci., 10.","DOI":"10.3390\/vetsci10010021"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Choi, H.-W., Kwon, Y.J., Park, J.-H., Lee, S.-Y., Chun, S., Won, E.J., Lee, J.H., Choi, H.-J., Kim, S.H., and Shin, M.-G. (2020). Evaluation of a fully automated Antinuclear Antibody Indirect Immunofluorescence assay in routine use. Front. Immunol., 11.","DOI":"10.3389\/fimmu.2020.607541"},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Stuen, S., Granquist, E.G., and Silaghi, C. (2013). Anaplasma phagocytophilum\u2014A widespread multi-host pathogen with highly adaptive strategies. Front. Cell. Infect. Microbiol., 3.","DOI":"10.3389\/fcimb.2013.00031"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1016\/j.emc.2024.01.004","article-title":"Tick-Borne Diseases","volume":"42","author":"Eilbert","year":"2024","journal-title":"Emerg. Med. Clin. N. Am."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"e00083-18","DOI":"10.1128\/CMR.00083-18","article-title":"Emerging tick-borne diseases","volume":"33","author":"Kramer","year":"2020","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1186\/s13071-023-06010-w","article-title":"One Health approach to toxocariasis in Quilombola communities of Southern Brazil","volume":"16","author":"Panazzolo","year":"2023","journal-title":"Parasit. Vectors"},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Panazzolo, G.K., Kmetiuk, L.B., Domingues, O.J., Farinhas, J.H., Doline, F.R., de Fran\u00e7a, D.A., Rodrigues, N.J.L., Biondo, L.M., Giuffrida, R., and Langoni, H. (2023). One Health approach in serosurvey of Toxoplasma gondii in former black slave (Quilombola) communities in Southern Brazil and among their dogs. Trop. Med. Infect. Dis., 8.","DOI":"10.3390\/tropicalmed8070377"},{"key":"ref_34","doi-asserted-by":"crossref","unstructured":"de Fran\u00e7a, D.A., Kmetiuk, L.B., Panazzolo, G.A.K., Domingues, O.J., da Silva, F.P., Biondo, L.M., de Souza Ribeiro Mioni, M., Possebon, F.S., de Lima Dur\u00e9, A.\u00cd., and Silva, M.V.F. (2024). Serosurvey of Coxiella burnetii in Descendants of Former Black Slaves (Quilombola Communities) of Southern Brazil. Microorganisms, 12.","DOI":"10.3390\/microorganisms12010092"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Pettan-Brewer, C., Figueroa, D.P., Cediel-Becerra, N., Kahn, L.H., Martins, A.F., and Biondo, A.W. (2022). Editorial: Challenges and successes of One Health in the context of Planetary Health in Latin America and the Caribbean. Front. Public Health, 10.","DOI":"10.3389\/fpubh.2022.1081067"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e00206-2024","DOI":"10.1590\/0037-8682-0334-2024","article-title":"Dengue mimickers: Which clinical conditions can resemble Dengue Fever?","volume":"57","author":"Farias","year":"2024","journal-title":"Rev. Soc. Bras. Med. Trop."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.pt.2015.09.004","article-title":"Best Practices for preventing vector-borne diseases in dogs and humans","volume":"32","author":"Otranto","year":"2016","journal-title":"Trends Parasitol."}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/4\/318\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:00:20Z","timestamp":1760029220000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/4\/318"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,26]]},"references-count":37,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["pathogens14040318"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14040318","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,26]]}}}