{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:41:29Z","timestamp":1791618089423,"version":"4.3.4"},"reference-count":47,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T00:00:00Z","timestamp":1744243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001503","name":"Indian Council of Agricultural Research","doi-asserted-by":"publisher","award":["AS\/1\/1\/12017-ASR-IV"],"award-info":[{"award-number":["AS\/1\/1\/12017-ASR-IV"]}],"id":[{"id":"10.13039\/501100001503","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Brucellosis, a contagious reproductive disease of livestock, has a significant economic impact in terms of abortions and stillbirths and has zoonotic importance. A study was conducted to estimate the seroprevalence of brucellosis in a bovine population in Haryana state, India. This study was carried out on 4325 bovine serum samples (Cattle: 2151, Buffalo: 2174) using the Rose Bengal Plate Test (RBPT) and indirect enzyme-linked immunosorbent assay (i-ELISA). The seroprevalence, estimated individually by the RBPT and i-ELISA, was found to be 6.86% (95% CI: 6.11\u20137.62) and 6.05% (95% CI: 5.34\u20136.76), respectively. In total, 258 out of 4325 (5.96%; 95% CI: 5.25\u20136.67) samples were found to be positive by both assays. The prevalence was found to be significantly higher in the cattle population (7.58%) as compared to buffalo (4.37%) (Chi-square = 19, p &lt; 0.001). Seroprevalence was highest in the agroclimatic zone I (8.73%), followed by zone II (7.33%) and zone III (1.45%) (Chi-square = 76.27, p &lt; 0.001).<\/jats:p>","DOI":"10.3390\/pathogens14040373","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T05:28:07Z","timestamp":1744262887000},"page":"373","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Seroprevalence of Brucellosis in Haryana, India: A Study Using Rose Bengal Plate Test and Enzyme-Linked Immunosorbent Assay"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9510-247X","authenticated-orcid":false,"given":"Dinesh","family":"Mittal","sequence":"first","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0792-3564","authenticated-orcid":false,"given":"Kushal","family":"Grakh","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Punit","family":"Jhandai","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0991-1372","authenticated-orcid":false,"given":"Swati","family":"Dahiya","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renu","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0911-4517","authenticated-orcid":false,"given":"Ramesh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anand","family":"Prakash","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pankaj","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pallavi","family":"Moudgil","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh","family":"Khurana","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naresh","family":"Jindal","sequence":"additional","affiliation":[{"name":"Department of Veterinary Public Health and Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar 125004, Haryana, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/S1286-4579(03)00091-1","article-title":"Classification of Brucella strains isolated from marine mammals by infrequent restriction site PCR and development of specific PCR identification tests","volume":"5","author":"Cloeckaert","year":"2003","journal-title":"Microb. Infect."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/j.ijid.2015.06.027","article-title":"Brucellosis in pregnancy: Clinical aspects and obstetric outcomes","volume":"38","author":"Vilchez","year":"2015","journal-title":"Int. J. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Makita, K., F\u00e8vre, E.M., Waiswa, C., Eisler, M.C., Thrusfield, M., and Welburn, S.C. (2011). Herd prevalence of bovine brucellosis and analysis of risk factors in cattle in urban and peri-urban areas of the Kampala economic zone, Uganda. BMC Public Health., 7.","DOI":"10.1186\/1746-6148-7-60"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Mathew, C., Stokstad, M., Johansen, T.B., Klevar, S., Mdegela, R.H., Mwamengele, G., Michel, P., Escobar, L., Fretin, D., and Godfroid, J. (2015). First isolation, identification, phenotypic and genotypic characterization of Brucella abortus biovar 3 from dairy cattle in Tanzania. BMC Vet. Res., 11.","DOI":"10.1186\/s12917-015-0476-8"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1590\/S0100-736X2013000600012","article-title":"Economic losses due to bovine brucellosis in Brazil","volume":"33","author":"Santos","year":"2013","journal-title":"Pesqui. Vet. Bras."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.prevetmed.2015.03.013","article-title":"Economic losses occurring due to brucellosis in Indian livestock populations","volume":"119","author":"Singh","year":"2015","journal-title":"Prev. Vet. Med."},{"key":"ref_7","first-page":"1556548","article-title":"Bovine brucellosis: Prevalence, risk factors, economic cost and control options with particular reference to India: A review","volume":"8","author":"Deka","year":"2018","journal-title":"Infect. Ecol. Epidemiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"100367","DOI":"10.1016\/j.onehlt.2021.100367","article-title":"Brucellosis in India: Comparing exposure amongst veterinarians","volume":"14","author":"Tiwari","year":"2022","journal-title":"One Health"},{"key":"ref_9","unstructured":"NADCP (2025, February 03). National Animal Disease Control Programme (Nadcp), Available online: https:\/\/www.dahd.gov.in\/sites\/default\/files\/2024-11\/NADCPOperationalGuidelines31-12-2019.pdf."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"222","DOI":"10.14202\/vetworld.2020.222-230","article-title":"Perceptions and preparedness of veterinarians to combat brucellosis through brucellosis control Programme in India","volume":"13","author":"Shome","year":"2020","journal-title":"Vet. World"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1128\/CMR.00073-19","article-title":"Laboratory diagnosis of human brucellosis","volume":"33","author":"Yagupsky","year":"2019","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_12","first-page":"39","article-title":"Performance of agriculture and allied sectors in Haryana","volume":"5","author":"Sharma","year":"2014","journal-title":"J. Econ. Sustain. Dev."},{"key":"ref_13","first-page":"92","article-title":"Laboratory techniques in brucellosis","volume":"55","author":"Alton","year":"1967","journal-title":"Monogr. Ser. World Health Organ."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1007\/s11250-013-0362-y","article-title":"Herd and individual animal prevalence of bovine brucellosis with associated risk factors on dairy farms in Haryana and Punjab in India","volume":"45","author":"Chand","year":"2013","journal-title":"Trop. Anim. Health Prod."},{"key":"ref_15","first-page":"1","article-title":"Prevalence of bovine brucellosis in India: A meta-analysis","volume":"43","author":"Jaismon","year":"2023","journal-title":"Vet. Q."},{"key":"ref_16","first-page":"448","article-title":"Seroprevalence of bovine brucellosis in Haryana by avidin-biotin serum ELISA and its comparison with RBPT and SAT","volume":"82","author":"Khurana","year":"2012","journal-title":"Indian J. Anim. Sci."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1977","DOI":"10.20546\/ijcmas.2020.907.226","article-title":"Brucellosis and associated risk factors analysis in serum sample of bovines","volume":"9","author":"Mishra","year":"2020","journal-title":"Int. J. Curr. Microbiol. App. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Meena, D.S., Sharma, L., Bishnoi, J., Soni, M., Jeph, N.K., Galav, V., and Sharma, S.K. (2023). Serological and molecular prevalence of Brucella spp. among livestock species in Rajasthan, India. Front. Vet. Sci., 10.","DOI":"10.3389\/fvets.2023.1157211"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"559","DOI":"10.20546\/ijcmas.2019.808.067","article-title":"Serosurveillance of bovine brucellosis using RBPT and c-ELISA and comparative evaluation of test performance","volume":"8","author":"Yuguda","year":"2019","journal-title":"Int. J. Curr. Microbiol. App. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Shakuntala, I., Milton, A.A.P., Sanjukta, R.K., Kakoty, K., Karam, A., Dutta, A., Puro, K., Sen, A., Das, S., and Ghatak, S. (2021). Isolation and Sero-Genomo-epidemiological studies on Brucella infection in dairy cattle in Meghalaya, India. Comp. Immunol. Microbiol. Infect. Dis., 78.","DOI":"10.1016\/j.cimid.2021.101694"},{"key":"ref_21","first-page":"61","article-title":"Comparison of Rose Bengal plate agglutination, standard tube agglutination and indirect ELISA tests for detection of Brucella antibodies in cows and buffaloes","volume":"3","author":"Ghodasara","year":"2010","journal-title":"Vet. World"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"379","DOI":"10.17221\/1555-VETMED","article-title":"Prevalence of brucellosis in ruminants in Bangladesh","volume":"56","author":"Rahman","year":"2011","journal-title":"Vet. Med."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"175","DOI":"10.36062\/ijah.57.2.2018.175-182","article-title":"Seroprevalence patterns of bovine brucellosis in organised and unorganised farms of coastal Andhra Pradesh, India","volume":"57","author":"Deepthi","year":"2018","journal-title":"Indian J. Anim. Hlth."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Holt, H.R., Bedi, J.S., Kaur, P., Mangtani, P., Sharma, N.S., Gill, J.P.S., Singh, Y., Kumar, R., Kaur, M., and McGiven, J. (2021). Epidemiology of brucellosis in cattle and dairy farmers of rural Ludhiana, Punjab. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009102"},{"key":"ref_25","first-page":"454","article-title":"Sero-prevalence of brucellosis in food animals in the Punjab, Pakistan","volume":"34","author":"Gul","year":"2014","journal-title":"Pak. Vet. J."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"577","DOI":"10.3923\/ajava.2015.577.583","article-title":"Seroprevalence of bovine brucellosis in different agro-climatic regions of Punjab","volume":"10","author":"Zadon","year":"2015","journal-title":"Asian J. Anim. Vet. Adv."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.20546\/ijcmas.2019.811.201","article-title":"Seroprevalence of brucellosis in bovine","volume":"8","author":"Shrimali","year":"2019","journal-title":"Int. J. Curr. Microbiol. App. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"963","DOI":"10.56093\/ijans.v90i7.106638","article-title":"Serological evidence of Brucellosis in selected gaushalas of Haryana","volume":"90","author":"Saidu","year":"2020","journal-title":"Indian J. Anim. Sci."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lukambagire, A.S., Mendes, A.J., Bodenham, R.F., McGiven, J.A., Mkenda, N.A., Mathew, C., Rubach, M.P., Sakasaka, P., Shayo, D.D., and Maro, V.P. (2021). Performance characteristics and costs of serological tests for brucellosis in a pastoralist Community of Northern Tanzania. Sci. Rep., 11.","DOI":"10.1038\/s41598-021-82906-w"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"272","DOI":"10.7883\/yoken.64.272","article-title":"Evaluation of serological tests for diagnosis of brucellosis","volume":"64","author":"Pabuccuoglu","year":"2011","journal-title":"Jpn. J. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/S0167-5877(98)00080-4","article-title":"Evaluation of an indirect ELISA for the diagnosis of bovine brucellosis in Milk and serum samples in dairy cattle in Argentina","volume":"36","author":"Vanzini","year":"1998","journal-title":"Prev. Vet. Med."},{"key":"ref_32","first-page":"913","article-title":"Specificity dependence between serological tests for diagnosing bovine brucellosis in Brucella-free farms showing false positive serological reactions due to Yersinia enterocolitica O:9","volume":"46","author":"Munoz","year":"2005","journal-title":"Can. Vet. J."},{"key":"ref_33","unstructured":"World Organization for Animal Health (2018). Q Fever. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals, World Organization for Animal Health."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"296","DOI":"10.3325\/cmj.2010.51.296","article-title":"Diagnosis of brucellosis in livestock and wildlife","volume":"51","author":"Godfroid","year":"2010","journal-title":"Croatian Med. J."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Legesse, A., Mekuriaw, A., Gelaye, E., Abayneh, T., Getachew, B., Weldemedhin, W., and Birhanu, K. (2023). Comparative evaluation of RBPT, I-ELISA, and CFT for the diagnosis of brucellosis and PCR detection of Brucella species from Ethiopian sheep, goats, and cattle sera. BMC Microbiol., 23.","DOI":"10.1186\/s12866-023-02962-2"},{"key":"ref_36","first-page":"11","article-title":"Situation of brucellosis in bovines at organized cattle farms belonging to three different states","volume":"6","author":"Chand","year":"2004","journal-title":"J. Immunol. Immunopathol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1111\/j.1348-0421.2004.tb03553.x","article-title":"Epidemiological and serological survey of brucellosis in Mongolia by ELISA using sarcosine extracts","volume":"48","author":"Erdenebaatar","year":"2004","journal-title":"Microbiol. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"46","DOI":"10.2174\/1874318801004010046","article-title":"Diagnosis of brucellosis","volume":"4","author":"Poester","year":"2010","journal-title":"Open Vet. Sci. J."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Muthiah, D., Singh, M., Chhabra, R., Gupta, R., Dahiya, S., and Manoj, J. (2024). Seroprevalance of Brucellosis and Q-fever in water buffaloes (Bubalus bubalis) in Haryana state, India. PLoS ONE, 19.","DOI":"10.1371\/journal.pone.0314726"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"244","DOI":"10.5455\/ijlr.20170915054130","article-title":"Seroprevalence of brucellosis in buffaloes by indirect enzyme linked immune-sorbent assay in Punjab, India","volume":"8","author":"Islam","year":"2018","journal-title":"Int. J. Livestock Res."},{"key":"ref_41","first-page":"43","article-title":"Serological survey of brucellosis in cattle and buffaloes of Jodhpur Region","volume":"6","author":"Subash","year":"2005","journal-title":"India Vet. Pract."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1093\/trstmh\/trz133","article-title":"The prevalence and risk factors for human Brucella species infection in a cross-sectional survey of a rural population in Punjab, India","volume":"114","author":"Mangtani","year":"2020","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"393","DOI":"10.2754\/avb200877030393","article-title":"A study on the epidemiology of bovine brucellosis in Punjab (India) using milk-ELISA","volume":"77","author":"Aulakh","year":"2008","journal-title":"Acta Vet. Brno."},{"key":"ref_44","first-page":"358950","article-title":"Prevalence of bovine brucellosis in organized dairy farms, using milk ELISA, in Quetta city, Balochistan, Pakistan","volume":"1","author":"Shafee","year":"2011","journal-title":"Vete. Med. Int."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"550","DOI":"10.5455\/vetworld.2013.550-553","article-title":"Seroprevalence studies of bovine brucellosis using indirect enzyme-linked immunosorbent assay (i-ELISA) at organized and unorganized farms in three different states of India","volume":"6","author":"Jagapur","year":"2013","journal-title":"Vet. World"},{"key":"ref_46","unstructured":"Radostits, O.M., Gay, C.C., Hinchliff, K.W., and Constable, P.D. (2025, February 04). Veterinary Medicine: A Text Book of Diseases of Cattle, Sheep, Pigs, Goats and Horses, Available online: https:\/\/pmc.ncbi.nlm.nih.gov\/articles\/PMC2857440\/."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Khan, M.Z., and Zahoor, M. (2018). An overview of brucellosis in cattle and humans, and its serological and molecular diagnosis in control strategies. Trop. Med. Infect. Dis., 3.","DOI":"10.3390\/tropicalmed3020065"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/4\/373\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:12:00Z","timestamp":1760029920000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/4\/373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,10]]},"references-count":47,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["pathogens14040373"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14040373","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,10]]}}}