{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T06:07:35Z","timestamp":1791871655169,"version":"4.3.4"},"reference-count":50,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T00:00:00Z","timestamp":1744588800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Newcastle disease viruses (NDVs) circulating among wild birds and poultry may differ in virulence. Some NDVs cause devastating outbreaks in chickens. The NDV\/duck\/Moscow\/3639\/2008 (d3639) strain was isolated from a wild duck. Its genome was sequenced (PP795281, GenBank) and the biological properties, specifically for infection in chicken and mice, were studied. Strain d3639 of genotype I.2 has an F protein cleavage site (112-GKQGRL-117) and a HN protein length (616 a.a.) of the lentogenic pathotype. It was tested, in comparison with the genotype II LaSota vaccine strain, for its immunogenicity and protective efficacy against a challenge with the velogenic NDV strain NDV\/chicken\/Moscow\/6081\/2022 (ch6081) of sub-genotype VII.1.1, the complete genome of which was also sequenced in this study (PP766718, GenBank). Both the d3639 and LaSota viruses did not induce clinical signs in chickens or mice. Single immunization was performed by inoculation through drinking water with the live virus. Inoculation protected the chickens during a subsequent challenge with velogenic ch6081 and significantly reduced shedding in feces. Double immunization was sufficient to achieve prolonged immunity and prevented the shedding of the velogenic virus after the challenge. Thus, this natural lentogenic d3639 virus possesses properties similar to the LaSota vaccine strain and can protect against sub-genotype VII.1.1 NDV.<\/jats:p>","DOI":"10.3390\/pathogens14040380","type":"journal-article","created":{"date-parts":[[2025,4,15]],"date-time":"2025-04-15T03:59:06Z","timestamp":1744689546000},"page":"380","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Genotype I Newcastle Disease Virus, Isolated from Wild Duck, Can Protect Chickens Against Newcastle Disease Caused by Genotype VII"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8491-4640","authenticated-orcid":false,"given":"Elizaveta","family":"Boravleva","sequence":"first","affiliation":[{"name":"Chumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement \u201cMoskovskiy\u201d, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3801-2413","authenticated-orcid":false,"given":"Anastasia","family":"Treshchalina","sequence":"additional","affiliation":[{"name":"Chumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement \u201cMoskovskiy\u201d, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria","family":"Gordeeva","sequence":"additional","affiliation":[{"name":"Chumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement \u201cMoskovskiy\u201d, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1892-0548","authenticated-orcid":false,"given":"Alexandra","family":"Gambaryan","sequence":"additional","affiliation":[{"name":"Chumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement \u201cMoskovskiy\u201d, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alla","family":"Belyakova","sequence":"additional","affiliation":[{"name":"Chumakov Federal Scientific Center for the Research and Development of Immune-and-Biological Products, Village of Institute of Poliomyelitis, Settlement \u201cMoskovskiy\u201d, 108819 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Gafarova","sequence":"additional","affiliation":[{"name":"The Scriabin and Kovalenko Federal Scientific Center for All-Russian Scientific Research Institute of Experimental Veterinary, The Russian Academy of Sciences, 109428 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexey","family":"Prilipov","sequence":"additional","affiliation":[{"name":"The Gamaleya National Center of Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Galina","family":"Sadykova","sequence":"additional","affiliation":[{"name":"The Gamaleya National Center of Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Adams","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Lausanne University Hospital, 1011 Lausanne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana","family":"Timofeeva","sequence":"additional","affiliation":[{"name":"The Gamaleya National Center of Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2638-4244","authenticated-orcid":false,"given":"Natalia","family":"Lomakina","sequence":"additional","affiliation":[{"name":"The Gamaleya National Center of Epidemiology and Microbiology of the Russian Ministry of Health, 123098 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,14]]},"reference":[{"key":"ref_1","unstructured":"ICTV (2025, April 11). Virus Taxonomy: Release. Available online: https:\/\/ictv.global\/taxonomy."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Miller, P.J., Dimitrov, K.M., Williams-Coplin, D., Peterson, M.P., Pantin-Jackwood, M.J., Swayne, D.E., Suarez, D.L., and Afonso, C.L. (2015). International Biological Engagement Programs Facilitate Newcastle Disease Epidemiological Studies. Front. Public. Health, 3.","DOI":"10.3389\/fpubh.2015.00235"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Ross, C.S., Mahmood, S., Skinner, P., Mayers, J., Reid, S.M., Hansen, R.D.E., and Banyard, A.C. (2022). JMM Profile: Avian paramyxovirus type-1 and Newcastle disease: A highly infectious vaccine-preventable viral disease of poultry with low zoonotic potential. J. Med. Microbiol., 71.","DOI":"10.1099\/jmm.0.001489"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1080\/03079457.2012.697991","article-title":"The long view: A selective review of 40 years of Newcastle disease research","volume":"41","author":"Alexander","year":"2012","journal-title":"Avian Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1637\/7381-051605R.1","article-title":"Molecular characterization and phylogenetic analysis of Newcastle disease virus isolates from healthy wild birds","volume":"49","author":"Zanetti","year":"2005","journal-title":"Avian Dis."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.virusres.2014.02.016","article-title":"Newcastle disease virus: Current status and our understanding","volume":"184","author":"Ganar","year":"2014","journal-title":"Virus Res."},{"key":"ref_7","unstructured":"(2025, April 11). Newcastle Disease (Infection with Newcastle Disease Virus) (Version Adopted in May 2021). Available online: https:\/\/www.woah.org\/fileadmin\/Home\/fr\/Health_standards\/tahm\/3.03.14_NEWCASTLE_DIS.pdf."},{"key":"ref_8","unstructured":"World Organization for Animal Health (WOAH) (2025, January 28). Newcastle Disease. Available online: https:\/\/www.woah.org\/en\/disease\/newcastle-disease\/."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Ul-Rahman, A., Shabbir, M.A.B., and Raza, M.A. (2021). Newcastle disease in mammalian species: The propensity of crossspecies transmission towards public health perspective. Veterinary Pathobiology And Public Health, Unique Scientific Publishers. Available online: https:\/\/uniquescientificpublishers.com\/wp-content\/uploads\/2021\/10\/451-461.pdf.","DOI":"10.47278\/book.vpph\/2021.038"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"103917","DOI":"10.1016\/j.meegid.2019.103917","article-title":"Updated unified phylogenetic classification system and revised nomenclature for Newcastle disease virus","volume":"74","author":"Dimitrov","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.vetmic.2016.12.019","article-title":"Newcastle disease vaccines\u2014A solved problem or a continuous challenge?","volume":"206","author":"Dimitrov","year":"2017","journal-title":"Vet. Microbiol."},{"key":"ref_12","unstructured":"(2025, April 11). Instructions for Newcastle Disease Control: Approved by the Chief Veterinary Department of the Ministry of Agriculture of the USSR on 09.06.1976 (Amended on 28.08.1978). (In Russian)."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"297","DOI":"10.2307\/1589696","article-title":"Historical note on the origin of the LaSota strain of Newcastle disease virus","volume":"24","author":"Goldhaft","year":"1980","journal-title":"Avian Dis."},{"key":"ref_14","first-page":"328","article-title":"Evaluating the efficacy of LaSota vaccination induced protection in chickens upon challenge with a genotype IV strain of Newcastle disease virus","volume":"28","author":"Bhuvaneswari","year":"2017","journal-title":"Virus Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1016\/j.vaccine.2016.11.048","article-title":"Antigenic variation of LaSota and genotype VII Newcastle disease virus (NDV) and their efficacy against challenge with velogenic NDV","volume":"35","author":"Yang","year":"2017","journal-title":"Vaccine"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Elbestawy, A., Ellakany, H., Sedeik, M., Gado, A., Abdel-Latif, M., Noreldin, A., Orabi, A., Radwan, I., and El-Ghany, W.A. (2023). Superior Efficacy of Apathogenic Genotype I (V4) over Lentogenic Genotype II (LaSota) Live Vaccines against Newcastle Disease Virus Genotype VII.1.1 in Pathogen-Associated Molecular Pattern-H9N2 Vaccinated Broiler Chickens. Vaccines, 11.","DOI":"10.20944\/preprints202309.0718.v1"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Chang, Z., Dong, X., Guan, Z., Lu, K., Chen, X., Wei, X., Guo, H., Dang, R., Wang, J., and Wang, X. (2023). Antigenic variation in hemagglutinin-neuraminidase of Newcastle disease virus isolated from Tibet, China. Vet. Microbiol., 285.","DOI":"10.1016\/j.vetmic.2023.109872"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Wang, N., Huang, M., Fung, T.S., Luo, Q., Ye, J.X., Du, Q.R., Wen, L.H., Liu, D.X., and Chen, R.A. (2020). Rapid Development of an Effective Newcastle Disease Virus Vaccine Candidate by Attenuation of a Genotype VII Velogenic Isolate Using a Simple Infectious Cloning System. Front. Vet. Sci., 7.","DOI":"10.3389\/fvets.2020.00648"},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Rtishchev, A., Treshchalina, A., Shustova, E., Boravleva, E., and Gambaryan, A. (2023). An Outbreak of Newcastle Disease Virus in the Moscow Region in the Summer of 2022. Vet. Sci., 10.","DOI":"10.20944\/preprints202306.0279.v1"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Amoia, C.F., Hakizimana, J.N., Chengula, A.A., Munir, M., Misinzo, G., and Weger-Lucarelli, J. (2024). Genomic Diversity and Geographic Distribution of Newcastle Disease Virus Genotypes in Africa: Implications for Diagnosis, Vaccination, and Regional Collaboration. Viruses, 16.","DOI":"10.3390\/v16050795"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"e3","DOI":"10.4142\/jvs.23022","article-title":"Newcastle disease virus: The past and current situation in Indonesia","volume":"25","author":"Dharmayanti","year":"2024","journal-title":"J. Vet. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2086","DOI":"10.14202\/vetworld.2023.2086-2095","article-title":"Detection and molecular characterization of virulent Newcastle disease virus (subgenotype VII.2) in broiler chickens in Northern Vietnam","volume":"16","author":"Tran","year":"2023","journal-title":"Vet. World"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"44","DOI":"10.29326\/2304-196X-2021-1-36-44-51","article-title":"Effectiveness of vaccines produced by the Federal State-Financed Institution \u201cARRIAH\u201d against topical genotype VII Newcastle disease viruses","volume":"1","author":"Frolov","year":"2021","journal-title":"Vet. Sci. Today"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"76","DOI":"10.29326\/2304-196X-2020-2-33-76-82","article-title":"Serological monitoring of avian in\u00a3uenza and Newcastle disease in the Russian Federation in_2019","volume":"2","author":"Volkova","year":"2020","journal-title":"Vet. Sci. Today"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Murashkina, T., Sharshov, K., Gadzhiev, A., Petherbridge, G., Derko, A., Sobolev, I., Dubovitskiy, N., Loginova, A., Kurskaya, O., and Kasianov, N. (2024). Avian Influenza Virus and Avian Paramyxoviruses in Wild Waterfowl of the Western Coast of the Caspian Sea (2017\u20132020). Viruses, 16.","DOI":"10.3390\/v16040598"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Treshchalina, A., Postnikova, Y., Gambaryan, A., Ishmukhametov, A., Prilipov, A., Sadykova, G., Lomakina, N., and Boravleva, E. (2022). Monitoring of Avian Influenza Viruses and Paramyxoviruses in Ponds of Moscow and the Moscow Region. Viruses, 14.","DOI":"10.3390\/v14122624"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nprot.2006.83","article-title":"The single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction: Twenty-something years on","volume":"1","author":"Chomczynski","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1126\/science.122.3160.156.b","article-title":"Identification of vaccine strains of Newcastle disease virus","volume":"122","author":"Hanson","year":"1955","journal-title":"Science"},{"key":"ref_29","first-page":"58","article-title":"Development of an inexpensive and simple test system for the differential detection of avian influenza viruses and avian paramyxoviruses in environmental monitoring","volume":"4","author":"Boravleva","year":"2024","journal-title":"J. Res. Vet. Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1006\/viro.2001.1123","article-title":"Mutational analysis of the membrane proximal heptad repeat of the newcastle disease virus fusion protein","volume":"289","author":"McGinnes","year":"2001","journal-title":"Virology"},{"key":"ref_31","first-page":"310","article-title":"Prediction of glycosylation across the human proteome and the correlation to protein function","volume":"7","author":"Gupta","year":"2002","journal-title":"Pac. Symp. Biocomput."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1006\/viro.2001.1172","article-title":"Cloning, expression, and crystallization of the fusion protein of Newcastle disease virus","volume":"290","author":"Chen","year":"2001","journal-title":"Virology"},{"key":"ref_33","first-page":"157","article-title":"In silico prediction of B and T cell epitopes based on NDV fusion protein for vaccine development against Newcastle disease virus","volume":"12","author":"Hosseini","year":"2021","journal-title":"Vet. Res. Forum."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3105","DOI":"10.1099\/0022-1317-70-11-3105","article-title":"Location of neutralizing epitopes on the fusion protein of Newcastle disease virus strain Beaudette C","volume":"70","author":"Yusoff","year":"1989","journal-title":"J. Gen. Virol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1007\/s11262-013-0903-5","article-title":"HN gene C-terminal extension of Newcastle disease virus is not the determinant of the enteric tropism","volume":"47","author":"Zhao","year":"2013","journal-title":"Virus Genes"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1007\/s00705-023-05832-4","article-title":"C-terminal truncation of the hemagglutinin-neuraminidase (HN) protein enhances the virulence and immunogenicity of Newcastle disease virus (NDV) vaccine strain V4","volume":"168","author":"Ren","year":"2023","journal-title":"Arch. Virol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2115","DOI":"10.1099\/0022-1317-69-8-2115","article-title":"Location of a neutralizing epitope for the haemagglutinin-neuraminidase glycoprotein of Newcastle disease virus","volume":"69 Pt. 8","author":"Chambers","year":"1988","journal-title":"J. Gen. Virol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"4999","DOI":"10.1128\/jvi.65.9.4999-5006.1991","article-title":"Neutralization map of the hemagglutinin-neuraminidase glycoprotein of Newcastle disease virus: Domains recognized by monoclonal antibodies that prevent receptor recognition","volume":"65","author":"Iorio","year":"1991","journal-title":"J. Virol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1016\/j.vetmic.2009.07.029","article-title":"Identification of a variable epitope on the Newcastle disease virus hemagglutinin-neuraminidase protein","volume":"140","author":"Hu","year":"2010","journal-title":"Vet. Microbiol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1186\/s12985-020-01483-y","article-title":"Identification of a potential neutralizing linear epitope of hemagglutinin-neuraminidase in Newcastle disease virus","volume":"18","author":"Jin","year":"2021","journal-title":"Virol. J."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"6469","DOI":"10.1074\/jbc.275.9.6469","article-title":"Determination of the disulfide bond arrangement of Newcastle disease virus hemagglutinin neuraminidase. Correlation with a beta-sheet propeller structural fold predicted for paramyxoviridae attachment proteins","volume":"275","author":"Pitt","year":"2000","journal-title":"J. Biol. Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/s13567-018-0587-x","article-title":"Pathobiology of Avian avulavirus 1: Special focus on waterfowl","volume":"49","author":"Rehman","year":"2018","journal-title":"Vet. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1186\/s13567-022-01118-w","article-title":"Current situation and future direction of Newcastle disease vaccines","volume":"53","author":"Hu","year":"2022","journal-title":"Vet. Res."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"110591","DOI":"10.1016\/j.vetimm.2023.110591","article-title":"Efficacy of Newcastle disease LaSota vaccine-induced hemagglutination inhibition antibodies against challenges with heterologous virulent strains of genotypes VII and IX","volume":"259","author":"Liu","year":"2023","journal-title":"Vet. Immunol. Immunopathol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.vetmic.2012.05.003","article-title":"Newcastle disease virus outbreaks: Vaccine mismatch or inadequate application?","volume":"160","author":"Dortmans","year":"2012","journal-title":"Vet. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1637\/9633-122410-Reg.1","article-title":"Generation of a genotype VII Newcastle disease virus vaccine candidate with high yield in embryonated chicken eggs","volume":"55","author":"Hu","year":"2011","journal-title":"Avian. Dis."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s13337-019-00537-2","article-title":"Genotypes II and VIId-based inactivated Newcastle disease vaccine reduces virus shedding","volume":"30","author":"Mahmoud","year":"2019","journal-title":"Virusdisease"},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Bello, M.B., Mahamud, S.N.A., Yusoff, K., Ideris, A., Hair-Bejo, M., Peeters, B.P.H., and Omar, A.R. (2020). Development of an Effective and Stable Genotype-Matched Live Attenuated Newcastle Disease Virus Vaccine Based on a Novel Naturally Recombinant Malaysian Isolate Using Reverse Genetics. Vaccines, 8.","DOI":"10.3390\/vaccines8020270"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1007\/s11259-024-10590-y","article-title":"Development of a genotype-matched Newcastle disease DNA vaccine candidate adjuvanted with IL-28b for the control of targeted velogenic strains of Newcastle disease virus in Africa","volume":"49","author":"Amoia","year":"2024","journal-title":"Vet. Res. Commun."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Li, J., Ding, J., Guo, C., Xu, X., Shan, C., Qian, J., and Ding, Z. (2024). Development of a novel chimeric ND-GP cVLPs vaccine for the prevention of goose-derived Newcastle disease and gosling plague. Microorganisms, 12.","DOI":"10.3390\/microorganisms12112266"}],"container-title":["Pathogens"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/4\/380\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:14:33Z","timestamp":1760030073000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2076-0817\/14\/4\/380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,14]]},"references-count":50,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,4]]}},"alternative-id":["pathogens14040380"],"URL":"https:\/\/doi.org\/10.3390\/pathogens14040380","relation":{},"ISSN":["2076-0817"],"issn-type":[{"value":"2076-0817","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,14]]}}}