{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T14:11:01Z","timestamp":1770819061231,"version":"3.50.1"},"reference-count":76,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH R01 AI102918"},{"name":"Technical University of Mombasa"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Aedes aegypti is the primary vector of arboviral diseases such as dengue, chikungunya, yellow fever, and Zika, posing significant global health and economic challenges. The effective control of this mosquito species requires understanding its seasonality, feeding behavior, and ecological dynamics. Modern molecular techniques, such as amplicon metagenomic sequencing, provide insights into vector\u2013host interactions and feeding patterns. This study investigated the temporal variation of Ae. aegypti abundance and its blood meal sources in coastal and western Kenya over 16 months. A total of 64,360 mosquitoes were collected, with Ae. aegypti comprising 10.9% (7035\/64,360). Coastal sites had a higher proportion (64.7%) of Ae. aegypti than western Kenya. Seasonal variation in abundance was observed, with peaks during the long rainy season and decline during the dry season. Blood meal analysis identified 15 vertebrate hosts, with humans being the primary source (86.6\u201395.9%). Other hosts included domestic animals such as turkey, sheep, cow, goat, and chicken. These findings highlight the role of rainfall in arboviral disease transmission and Ae. aegypti\u2019s strong preference for human hosts. Additionally, this study demonstrates the cost-effectiveness of group testing for identifying blood meal sources, with implications for public health interventions.<\/jats:p>","DOI":"10.3390\/pathogens14050505","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T06:31:27Z","timestamp":1747809087000},"page":"505","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Temporal Variation and Human Host Predominance in Aedes aegypti from Coastal and Western Kenya: Insights from Pooled Blood Meal Metagenomics"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-7687-2005","authenticated-orcid":false,"given":"Kavinya","family":"Mwendwa","sequence":"first","affiliation":[{"name":"Department of Pure and Applied Sciences, Technical University of Mombasa, Mombasa 80100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9000-7008","authenticated-orcid":false,"given":"Francis","family":"Mutuku","sequence":"additional","affiliation":[{"name":"Department of Environment and Health Sciences, Technical University of Mombasa, Mombasa 80100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3041-2714","authenticated-orcid":false,"given":"Sammy","family":"Wambua","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Pwani University, Kilifi 80108, Kenya"},{"name":"Pwani University Biosciences Research Centre (PUBReC), Pwani University, Kilifi 80108, Kenya"},{"name":"Research and Conservation Support Society [RECOURSE], Kilifi 80108, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Makenzi","family":"Nzaro","sequence":"additional","affiliation":[{"name":"Department of Pure and Applied Sciences, Technical University of Mombasa, Mombasa 80100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bryson A.","family":"Ndenga","sequence":"additional","affiliation":[{"name":"Kenya Medical Research Institute, Centre for Global Health Research, Kisumu 40100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kennedy","family":"Agoi","sequence":"additional","affiliation":[{"name":"Department of Pure and Applied Sciences, Technical University of Mombasa, Mombasa 80100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Angelle D.","family":"LaBeaud","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Division of Infectious Diseases, Stanford University School of Medicine, Stanford, CA 94305-5139, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2871-3075","authenticated-orcid":false,"given":"Carren","family":"Bosire","sequence":"additional","affiliation":[{"name":"Department of Pure and Applied Sciences, Technical University of Mombasa, Mombasa 80100, Kenya"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"272","DOI":"10.1016\/j.micinf.2009.12.011","article-title":"The dengue vector Aedes aegypti: What comes next","volume":"12","author":"Jansen","year":"2010","journal-title":"Microbes Infect."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1186\/s13071-017-2271-9","article-title":"Characterization and productivity profiles of Aedes aegypti L., breeding habitats across rural and urban landscapes in western and coastal Kenya","volume":"10","author":"Ngugi","year":"2017","journal-title":"Parasites Vectors"},{"key":"ref_3","unstructured":"(2024, September 25). 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