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Previous studies on blood parasites such as Anaplasmataceae, hemoplasmas, piroplasmids, and Hepatozoon spp. in South American bats have revealed high genetic diversity. This study investigated the molecular occurrence of these agents in 278 bats of 32 species captured in the state of Acre in the Western Brazilian Amazon. Spleen DNA samples were screened by PCR for different pathogens and tested negative for Anaplasma spp., Ehrlichia spp., Neorickettsia spp., piroplasmids, and Hepatozoon spp. However, 84 of the 208 samples (40.4%) were positive for hemoplasmas based on the 16S rRNA gene, and 15 out of the 84 (17.85%) were positive for the 23S rRNA gene. Seventeen 16S rRNA sequences, corresponding to 12 genotypes, were grouped with hemotropic Mycoplasma sp. previously detected in bats from Brazil, Peru, and Belize. Three 23S rRNA sequences represent three distinct genotypes clustered with hemotropic Mycoplasma sp., previously detected in Desmodus rotundus. This is the first molecular report of hemoplasmas in six bat species, namely Dermanura cinereus, Lophostoma silviculum, Phyllostomus elongatus, Phyllostomus hastatus, Rhinophylla fischerae, and Sturnira tildae.<\/jats:p>","DOI":"10.3390\/pathogens14060527","type":"journal-article","created":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T20:26:50Z","timestamp":1748204810000},"page":"527","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Molecular Survey of Hemopathogens in Bats from the Western Brazilian Amazon"],"prefix":"10.3390","volume":"14","author":[{"given":"Abra\u00e3o Isaque da","family":"Silva","sequence":"first","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal CEP 14884-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliz Oliveira","family":"Franco","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal CEP 14884-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Cl\u00e1udia","family":"Calchi","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal CEP 14884-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco Chagas Bezerra dos","family":"Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Trypanosomatid Biology, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro CEP 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rair de Sousa","family":"Verde","sequence":"additional","affiliation":[{"name":"Graduate Program in Health and Sustainable Animal Production in the Amazon, Federal University of Acre, Rio Branco CEP 69920-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0375-2588","authenticated-orcid":false,"given":"Vict\u00f3ria Valente Califre","family":"de Mello","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal CEP 14884-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel Ant\u00f4nio Braga","family":"Lee","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal CEP 14884-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline Tostes","family":"Secato","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal CEP 14884-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9348-4814","authenticated-orcid":false,"given":"Clara Morato","family":"Dias","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal CEP 14884-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosangela Zacarias","family":"Machado","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal CEP 14884-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8740-052X","authenticated-orcid":false,"given":"Andr\u00e9 Luiz Rodrigues","family":"Roque","sequence":"additional","affiliation":[{"name":"Laboratory of Trypanosomatid Biology, Instituto Oswaldo Cruz (FIOCRUZ), Rio de Janeiro CEP 21040-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1713-5222","authenticated-orcid":false,"given":"Marcos Rog\u00e9rio","family":"Andr\u00e9","sequence":"additional","affiliation":[{"name":"Vector-Borne Bioagents Laboratory (VBBL), Department of Pathology, Reproduction and One Health, School of Agrarian and Veterinary Sciences, S\u00e3o Paulo State University, Jaboticabal CEP 14884-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e20191004","DOI":"10.1590\/0001-3765202020191004","article-title":"Updated and annotated checklist of recent mammals from Brazil","volume":"92","author":"Quintela","year":"2020","journal-title":"Ann. 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