{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T06:31:17Z","timestamp":1790836277172,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>E-cigarettes, a form of electronic nicotine delivery system (ENDS), have gained significant popularity, particularly among adolescents who often view vaping as a \u201ccool\u201d lifestyle choice. This growing trend has spurred extensive research on the effects of ENDS on both oral and systemic health. By synthesizing data from systematic reviews and meta-analyses, this umbrella review offers a comprehensive evaluation of the impact of e-cigarettes on oral biofilm accumulation and microbiota composition. A systematic search was conducted up to 12 March 2025, across PubMed\/MEDLINE, Google Scholar, Cochrane Library, and Scopus for studies published between 2015 and 2025. Ten studies met the eligibility criteria. The quality of the selected papers, as assessed using the AMSTAR 2 tool, ranged from moderate to high. The findings of this review suggest that e-cigarette use may contribute to dysbiosis in the oral microbiota and foster biofilm accumulation, thereby increasing the risk of oral diseases such as periodontitis, peri-implantitis, oral candidiasis, and caries. The findings also highlight the need for further research into the long-term effects of e-cigarette use on oral health. This review is registered with PROSPERO (CRD420251025639).<\/jats:p>","DOI":"10.3390\/pathogens14060578","type":"journal-article","created":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T08:18:43Z","timestamp":1749543523000},"page":"578","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["An Umbrella Review of E-Cigarettes\u2019 Impact on Oral Microbiota and Biofilm Buildup"],"prefix":"10.3390","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2138-1223","authenticated-orcid":false,"given":"Beatriz","family":"Panariello","sequence":"first","affiliation":[{"name":"School of Dental Medicine, Lake Erie College of Osteopathic Medicine, Bradenton, FL 34211, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-1441-8430","authenticated-orcid":false,"given":"Fabr\u00edzio","family":"Dias Panariello","sequence":"additional","affiliation":[{"name":"School of Medical Sciences, S\u00e3o Leopoldo Mandic, Campinas 13045-755, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8214-3351","authenticated-orcid":false,"given":"Ashminie","family":"Misir","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, Lake Erie College of Osteopathic Medicine, Bradenton, FL 34211, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliane Porto","family":"Barboza","sequence":"additional","affiliation":[{"name":"School of Dental Medicine, Lake Erie College of Osteopathic Medicine, Bradenton, FL 34211, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1177\/0022034519872475","article-title":"Novel Nicotine Delivery Systems","volume":"30","author":"Kumar","year":"2019","journal-title":"Adv. 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