{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T01:23:20Z","timestamp":1760059400720,"version":"build-2065373602"},"reference-count":78,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T00:00:00Z","timestamp":1749600000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jagiellonian University Medical College","award":["N42\/DBS\/000293","POIR.04.02.00-00-D023\/20"],"award-info":[{"award-number":["N42\/DBS\/000293","POIR.04.02.00-00-D023\/20"]}]},{"name":"Priority Research Area qLIFE under the Strategic Programme Excellence Initiative at Jagiellonian University","award":["N42\/DBS\/000293","POIR.04.02.00-00-D023\/20"],"award-info":[{"award-number":["N42\/DBS\/000293","POIR.04.02.00-00-D023\/20"]}]},{"name":"Smart Growth Operational Programme POIR 4.2","award":["N42\/DBS\/000293","POIR.04.02.00-00-D023\/20"],"award-info":[{"award-number":["N42\/DBS\/000293","POIR.04.02.00-00-D023\/20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathogens"],"abstract":"<jats:p>Mycobacterium abscessus complex (MABc) poses a major therapeutic challenge due to its intrinsic multidrug resistance and ability to form biofilms. This study evaluated the in vitro activity of three antimycobacterial agents\u2014bedaquiline, delamanid, and clofazimine\u2014on 20 clinical MABc isolates, including M. abscessus subsp. abscessus, massiliense, and bolletii, with a focus on biofilm-forming phenotypes. Biofilm analysis showed that the rough colony morphotypes were mostly weak biofilm formers, while the smooth and mixed morphotypes were predominantly moderate or strong biofilm formers. A statistically significant association was observed between the mixed colony morphology and strong biofilm formation (p = 0.032). Importantly, bedaquiline exhibited potent and consistent activity across all isolates, regardless of the biofilm-forming ability, with MIC values ranging from 0.125 to 1 \u00b5g\/mL. In contrast, delamanid and clofazimine showed limited efficacy, with MIC values exceeding 16 \u00b5g\/mL and 8 \u00b5g\/mL, respectively. These findings strongly support the role of bedaquiline as a promising core agent for future combination therapies targeting drug-resistant MABc infections, including biofilm-associated infections. Our results, among the first from Poland, highlight the critical need for incorporating novel agents such as bedaquiline into therapeutic strategies against this difficult-to-treat pathogen.<\/jats:p>","DOI":"10.3390\/pathogens14060582","type":"journal-article","created":{"date-parts":[[2025,6,11]],"date-time":"2025-06-11T11:18:40Z","timestamp":1749640720000},"page":"582","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the In Vitro Activity of Bedaquiline, Delamanid, and Clofazimine Against Mycobacterium abscessus Complex and Their Antibiofilm Potential"],"prefix":"10.3390","volume":"14","author":[{"given":"Katarzyna","family":"Kania","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Medical College, St. Medyczna 9, 30-688 Krakow, Poland"},{"name":"Laboratory of Microbiology, The St. John Paul II Specialist Hospital, St. Pradnicka 80, 31-202 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katarzyna","family":"W\u00f3jcik","sequence":"additional","affiliation":[{"name":"Malopolska Central Laboratory of Tuberculosis Diagnostics, The St. John Paul II Specialist Hospital, St. Ulanow 29, 31-455 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alicja","family":"Sk\u00f3rkowska","sequence":"additional","affiliation":[{"name":"BioImaging Laboratory, Center for the Development of Therapies for Civilization and Age-Related Diseases, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2756-3340","authenticated-orcid":false,"given":"Karolina","family":"Klesiewicz","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Jagiellonian University Medical College, St. Medyczna 9, 30-688 Krakow, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Victoria, L., Gupta, A., G\u00f3mez, J.L., and Robledo, J. 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